Labshake search
Citations for SouthernBiotech :
451 - 500 of 1259 citations for Rabbit Anti Human IgG+IgM+IgA Alexa Fluor 750 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... The following secondary antibodies were used: rabbit-anti mouse IgG-horseradish peroxidase (HRP; SouthernBiotech; dilution 1:10‘000) and goat-anti rabbit IgG-HRP (SouthernBiotech ...
-
bioRxiv - Cell Biology 2020Quote: ... and probed using the indicated primary antibodies (Table SX) and anti-mouse or anti-rabbit IgG-linked HRP secondary antibodies (Southern Biotech) followed by ECL (Luminata Crescendo or Forte ...
-
bioRxiv - Immunology 2024Quote: ... membranes were incubated with primary antibodies overnight at 4 °C and during 2 hours at room temperature with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit IgG (SouthernBiotech, USA). Membranes were then revealed using G:BOX Chemi XL1.4 Fluorescent & Chemiluminescent Imaging System (Syngene ...
-
bioRxiv - Biochemistry 2020Quote: ... the culture media were tested for their ACE2-Fc contents using a custom ELISA (anti-ACE2 antibody [Abcam, Cambridge, MA] for capturing and anti-human IgG-Fc-HRP [SouthernBiotech, Birmingham, AL] for detection). Clones with the highest expression of recombinant ACE2-Fc variants were selected ...
-
bioRxiv - Microbiology 2022Quote: ... Normal rabbit IgG (SouthernBiotech, Cat# 0111-01, 1:100) was used as negative controls ...
-
bioRxiv - Microbiology 2022Quote: ... Normal rabbit IgG (SouthernBiotech, Cat# 0111-01, 1:100) was used as negative controls ...
-
bioRxiv - Immunology 2020Quote: ... Biotinylated rabbit control IgG (Southern Biotech, Birmingham, AL, USA) was used as a negative control ...
-
bioRxiv - Microbiology 2023Quote: ... Normal rabbit IgG (SouthernBiotech, Cat# 0111-01, 1:100) was used as negative control ...
-
bioRxiv - Microbiology 2023Quote: ... formaldehyde fixed parasites were adhered to glass slides and blocked with 50% FCS in PBS for 45 min before staining with primary anti-VSG (1:10,000) and secondary Alexa Fluor (1:1,000) antibodies and mounting with DAPI Fluoromount-G® (Southern Biotech). Imaging was performed with an Axioscope 2 fluorescence microscope (Zeiss ...
-
bioRxiv - Microbiology 2023Quote: ... formaldehyde fixed parasites were adhered to glass slides and blocked with 50% FCS in PBS for 45 min before staining with primary anti-VSG (1:10,000) and secondary Alexa Fluor (1:1,000) antibodies and mounting with DAPI Fluoromount-G® (Southern Biotech). Imaging was performed with an Axioscope 2 widefield fluorescence microscope (Zeiss ...
-
bioRxiv - Immunology 2022Quote: ... Plates were then washed with TBST and incubated with secondary HRP-conjugated goat anti-human IgG (Southern Biotech, Birmingham, AL) at a 1:15,000 dilution for 45 minutes at room temperature ...
-
bioRxiv - Cancer Biology 2022Quote: ... followed by detection with a 1:10,000 dilution of goat anti-human IgG-HRP (Southern Biotech 2040-05, Birmingham, AL). Plates were developed for 5 minutes with TMB substrate (VWR 95059-286) ...
-
bioRxiv - Immunology 2020Quote: ... MagPlex beads were washed with PBST (PBS, 0.05% Tween-20) and incubated with anti-human IgG Fc-PE (Southern Biotech). Finally ...
-
bioRxiv - Immunology 2020Quote: ... washed thrice with PBST and detected with HRP-conjugated mouse anti-human IgG (Fc only) (Southern Biotech, Birmingham, AL, USA) antibody at a 1:2000 dilution ...
-
bioRxiv - Immunology 2022Quote: ... plates were washed with TBS containing 0.05% Tween-20 (TBST) and resuspended in 50 μL of Goat-anti-human IgG-PE (Southern Biotech). After 2 hours of incubation on a rotator at room temperature ...
-
bioRxiv - Immunology 2019Quote: A20 D1.3 cells were stained on ice for 10 min with anti-IgM-biotin (Southern Biotech) or HEL-biotin and washed with PBS ...
-
bioRxiv - Immunology 2021Quote: ... Wells were washed 5 times and secondary goat anti-IgM-HRP (Southern Biotech, cat# 1021-08) at 1:5000 dilution was incubated for 1 hr in the wells ...
-
bioRxiv - Immunology 2023Quote: ... plates were washed in wash buffer (PBS-0.05%Tween 20) and incubated with AP-conjugated secondary antibodies (anti-IgM, anti-IgG1, Southern Biotech). Plates were washed and followed by adding alkaline phosphatase (AP) ...
-
bioRxiv - Immunology 2024Quote: ... IgGs were revealed with goat anti-mouse biotinylated-IgG (Southern Biotech) followed by alkaline-phosphatase–conjugated streptavidin (Jackson) ...
-
bioRxiv - Immunology 2020Quote: ... coated with 100 µL/well of goat anti-mouse IgA (5 µg/mL Bic 0.1M, pH 9.6; Southern Biotech) were incubated with serial 3-fold dilutions of either fecal supernatant (100 µL ...
-
bioRxiv - Microbiology 2023Quote: ... bacteria were stained again for 30 min with 0.5 µg of FITC-conjugated anti-mouse IgA antibody (Southern Biotech) in 100 µl of staining buffer to refresh and harmonize the staining between batches ...
-
bioRxiv - Microbiology 2023Quote: ... 20 µl/well of a Phosphatase Alkaline-conjugated goat anti-mouse IgA (Southern Biotech, Imtec diagnostics, Ref: 1040-04), diluted 1/1000 in Dilution Buffer ...
-
bioRxiv - Biochemistry 2020Quote: ... diluted 1:1,000 in TBST followed by 30 min with rabbit anti-goat HRP IgG (SouthernBiotech, #6164-05) at a dilution of 1:2,000 ...
-
bioRxiv - Plant Biology 2023Quote: ... and the secondary antibody used against other primary antibodies was a goat anti-rabbit IgG (4052-05, SouthernBiotech).
-
bioRxiv - Microbiology 2022Quote: ... antibody was from Santa Cruz Biotechnology (Texas, USA). Goat anti-rabbit IgG-HRP (Cat No. 4030-05) was from Southern Biotech (Alabama, USA). 3-Amino 1 ...
-
bioRxiv - Immunology 2023Quote: ... Membranes were then incubated with HRP-conjugated goat anti-rabbit IgG (H+L) (1:4000 dilution; Southern Biotech) at RT for 1 h ...
-
bioRxiv - Microbiology 2022Quote: ... goat anti-rabbit and rabbit anti-goat antibodies (SouthernBiotech) were used as secondary antibodies with a dilution of 1:2,000.
-
bioRxiv - Immunology 2023Quote: ... or α-IgM (Southern Biotech Cat # 1020-08) were incubated for one hour at room temperature ...
-
bioRxiv - Immunology 2023Quote: ... beads were incubated with anti-IgG::PE or anti-IgG::AF488 (2.5 µg/mL, Southern Biotech) in PBS with 1% BSA for 15 min ...
-
bioRxiv - Biochemistry 2020Quote: ... and then incubation for 30 mins at room temperature with anti-human IgG HRP at 1:4000 (Southern Biotech #2040-05). 5 washes of TBST were done followed by development using 1-Step Ultra TMB-ELISA Substrate Solution (ThermoFisher Scientific ...
-
bioRxiv - Immunology 2022Quote: ... Plates were washed with water three times before applying 25 μl of secondary antibody (goat anti-human IgG Fc-Southern Biotech, 2048-04 ...
-
bioRxiv - Immunology 2022Quote: ... IgG bound to the rSARS-CoV-2 S protein was detected using a horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody (Southern Biotech) incubated for 1 hour ± 10 minutes at 24°C ± 2°C ...
-
bioRxiv - Microbiology 2021Quote: ... Plates were washed three times and incubated with the goat anti-human-IgG Fc secondary antibody conjugated with alkaline phosphatase (AP, Southern Biotech) at a 1:1000 dilution in blocking buffer for 1 h at room temperature ...
-
bioRxiv - Immunology 2022Quote: ... The plates were then washed with PBS containing 0.1% Tween-20 (PBS-T), and Alkaline Phosphatase-conjugated Goat Anti-Human IgG (dilution 1:500, catalog no. 2040-04) from Southern Biotech was added and incubated for 45 min at room temperature ...
-
bioRxiv - Immunology 2019Quote: ... the membrane was re-blocked with 5% BSA in 1x TBST for 2 hours at room temperature before probing with 1:5000 dilution of anti-human IgG-HRP (Southern Biotech) in 5% BSA/1x TBST for 1 hour at room temperature ...
-
bioRxiv - Immunology 2019Quote: ... Cells were washed with PBS–0.1% Tween 20 and incubated with mouse anti-human IgG Fc-HRP (Southern Biotech; 9040-05) for 1 hour ...
-
bioRxiv - Immunology 2019Quote: ... Plates were washed with PBS–0.1% Tween 20 and incubated with mouse anti-human IgG Fc-HRP (Southern Biotech; 9040-05) for 1 hour ...
-
bioRxiv - Microbiology 2020Quote: ... Antibody binding to the spike proteins was determined using a 1:2000 diluted HRP conjugated goat anti-human IgG (ITK Southern Biotech) for 1 h at RT and tetramethylbenzidine substrate (BioFX) ...
-
bioRxiv - Immunology 2021Quote: ... incubated for 1 hour at room temperature with mouse anti-human IgG conjugated to horse radish peroxidase (Southern Biotech, Birmingham, USA) diluted 1:5000 in blocking solution ...
-
bioRxiv - Cell Biology 2020Quote: ... Mouse/Human ads-HRP (4050-05, dilution 1:10000) and Goat Anti-Mouse IgG(H+L) Human ads-HRP (1031-05, dilution 1:10000) (Southern Biotech); sheep anti-TGN38 (AHR499G ...
-
bioRxiv - Immunology 2022Quote: ... The cells were then stained with 50 μL of the secondary mix (goat anti-human IgG-AF488 (SouthernBiotech, cat. #2040-30) and goat anti-mouse Alexa Fluor 488 IgG (H + L ...
-
bioRxiv - Immunology 2022Quote: ... The plates were then washed three times with water and 200 μl of goat anti-human IgG HRP secondary antibody (Southern Biotech) diluted to a ratio of 1:2000 in block buffer was added and incubated for 1 hr at room temperature followed by an hour of incubation ...
-
bioRxiv - Cell Biology 2023Quote: ... Surface-bound sLAP was detected with anti-human PE antibodies that bound directly to the Fc-IgG part of the chimeric sLAP proteins (SouthernBiotech, USA). PE signals were analyzed with FlowJo software (BD Biosciences ...
-
bioRxiv - Immunology 2023Quote: ... Beads were then washed with PBST and reacted for 30 minutes at room temperature with biotinylated goat anti-human IgG (Southern Biotech) or goat whole IgG containing anti-monkey IgA (Novus ...
-
bioRxiv - Immunology 2021Quote: ... goat anti-mouse IgG (Southern Biotech) diluted in blocking solution (1:5000) ...
-
bioRxiv - Immunology 2021Quote: ... goat anti-mouse IgG (Southern Biotech) diluted in blocking solution (1:1000 ...
-
bioRxiv - Immunology 2021Quote: ... and double-distilled water to remove cells before being incubated with alkaline phosphatase-conjugated anti-mouse IgM or anti-mouse IgG1 (Southern Biotech) for 2 h at room temperature ...
-
bioRxiv - Immunology 2020Quote: ... The following antibody cocktails were used for B cell (FITC anti-mouse CD43, Biolegend, 553270; PE Goat anti-mouse IgM, Southern Biotech, 1020-09 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Precomplexation of the anti-sialyl-LewisX antibody was performed with a 1:50 dilution of goat anti-mouse IgM-HRP (#1021-05, Southern Biotech) and 0.65 µg donkey anti-goat Alexa Fluor 555 (A21432 ...
-
bioRxiv - Microbiology 2022Quote: ... α-IgA (mAb 11-44-2, FITC, SouthernBiotech catalog no. 1165-02). Cells were stained with primary antibodies (1:50 dilution ...