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Citations for SouthernBiotech :
251 - 300 of 1092 citations for Mouse IgG2b Isotype Control Antibody A 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Plates were washed 5 times with PBS-T and incubated with goat anti-mouse IgG1 antibody conjugated with horseradish peroxidase (SouthernBiotech) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... was used except that plates were coated with anti-human kappa and lambda light chain specific mouse antibodies (Southern Biotech) at 1:1 ratio diluted to 1 μg/ml in PBS overnight ...
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bioRxiv - Microbiology 2023Quote: ... Bound antibodies were detected by using goat anti-mouse IgG conjugated to horseradish peroxidase (HRP; Southern Biotech, Birmingham, AL, USA) diluted 1:2000 in PBST and developed using 3,3’,5,5’-tetramethylbenzidine substrate (Thermo Scientific Pierce ...
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bioRxiv - Immunology 2022Quote: ... 100 µL of goat anti-mouse IgG -HRP (1:4,000, Southern Biotech, Birmingham, AL, USA) or goat anti-hamster IgG-HRP (1:32,000 ...
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bioRxiv - Microbiology 2022Quote: ... or mouse anti-hamster-IgG1-HRP (Southern Biotech, Cat# 1940–05, 1:8000 in PBST) for 2 h at RT ...
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bioRxiv - Microbiology 2021Quote: ... or mouse anti-hamster-IgG1-HRP (Southern Biotech, Cat# 1940-05, 1:8000 in PBST) for 2 h at RT ...
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bioRxiv - Microbiology 2020Quote: ... HRP-conjugated goat anti-mouse IgG (Southern Biotech, cat no:1030-05, 1:5000 dilution), HRP-conjuated goat anti-horse IgG (Bellancom life H15034,1:2000 dilution ...
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bioRxiv - Immunology 2022Quote: ... while biotin-labeled goat anti-mouse IgA Ab (1:5000, Southern Biotech, Cat#1040-08) plus HRP-conjugated streptavidin (1:7000 ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse/Human ads-HRP (4050-05, dilution 1:10000) and Goat Anti-Mouse IgG(H+L) Human ads-HRP (1031-05, dilution 1:10000) (Southern Biotech); sheep anti-TGN38 (AHR499G ...
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bioRxiv - Immunology 2024Quote: ... The secondary antibodies used for western blot analysis were goat anti-rabbit conjugated to horseradish peroxidase-conjugated (HRP) (#4050-05, diluted at 1:1000) and anti-mouse-HRP (#1031-05, diluted at 1:1000) from SouthernBiotech (USA).
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bioRxiv - Immunology 2020Quote: ... Plates were incubated with serially diluted mouse sera (50 µL, PBS 1% BSA, wt/vol, 2 h, RT) and standard mouse IgG (Southern Biotech #0107-10, prepared in duplicates) and washed (5x ...
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bioRxiv - Immunology 2021Quote: ... 1:300 for IgA.] HRP conjugated antibodies were all from Southern BioTech: IgM (#1021-05) ...
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bioRxiv - Neuroscience 2023Quote: ... USA) and Goat anti-collagen IV antibody (1:200, Southern Biotech, US), mouse anti Human Protein Gene Product 9.5 (PGP9.5 ...
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bioRxiv - Immunology 2019Quote: ... The a-ATX antibody was detected with an a-rabbit HRP conjugated antibody 1:2000 (4010-05, Southern Biotech, AL, USA). Colour was developed using TMB (3,3’,5,5’-Tetramethylbenzidine ...
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bioRxiv - Immunology 2023Quote: ... IgG3 was measured following 6 washes and a 1-h incubation with 100 μL/well 1:5,000 mouse anti-human biotin-conjugated IgG3 hinge (SouthernBiotech, Birmingham, Alabama, USA) then 6 washes and 1-h incubation with 100 μL/well 1:40,000 HRP-conjugated streptavidin (Jackson ImmunoResearch Labs) ...
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bioRxiv - Immunology 2021Quote: ... washing with wash buffer (1xPBS+0.05% Tween) and incubation with secondary antibody - Mouse-anti-monkey IgG-HRP (Southern Biotech, 4700-05) for 1h ...
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bioRxiv - Immunology 2020Quote: ... plates were coated with 2 µg/mL of goat anti-human kappa or goat anti-human lambda antibodies cross-absorbed against mouse IgG (Southern Biotech) at 4°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... Secondary antibodies used include goat anti-mouse IgG and goat anti rabbit-IgG both conjugated to horseradish peroxidase (HRP) (Southern Biotech). Forte Western HRP substrate (Millipore ...
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bioRxiv - Immunology 2021Quote: ... Bound IgA or IgM antibody was detected by incubation with anti-IgA-alkaline phosphatase (goat anti-mouse IgA (α chain-specific) (SouthernBiotech) or anti-IgM-alkaline phosphatase goat anti-mouse IgM (μ chain-specific ...
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bioRxiv - Immunology 2020Quote: An ELISA to detect anti-CCHFV Ig was performed as previously described (Hawman et al., 2019) with an anti-mouse Ig detection antibody (Southern Biotech) to detect all immunoglobulin isotypes or anti-mouse IgG or IgM (Southern Biotech ...
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bioRxiv - Immunology 2020Quote: ... followed by addition of HRP-conjugated goat anti-mouse IgG second antibody (catalog number 1030-05, Southern Biotech, Birmingham, AL, USA). TMB substrate was added and the plates read at OD 450 nm with a plate reader ...
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bioRxiv - Immunology 2022Quote: ... Hp-specific IgG1 was detected as previously described47 using plates coated with Hp extract and detected using rat anti-mouse IgG1-HRP antibody (Southern Biotech). NP-OVA specific IgG1 was detected as previously described48 using plates were coated with TNP(5)-BSA and detected using goat anti-mouse IgG1-HRP (Southern Biotech).
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bioRxiv - Immunology 2023Quote: ... was added to the wells for 1h followed by washing and incubation with horseradish peroxidase-linked goat anti-mouse IgG secondary antibody (Southern Biotech). The avidity index was calculated as ...
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bioRxiv - Immunology 2022Quote: ... NP-specific IgM and IgG3 antibodies were detected using biotinylated anti-mouse IgM- or IgG3-specific immunoglobulins followed by streptavidin-HRP (Southern Biotech) and developed with SIGMAFAST OPD tablets (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... 1×109 bacteria were washed with 1 ml ice-cold PBS and stained for 30 min at 4°C with 4 µg of FITC-conjugated anti-mouse IgA antibody (Southern Biotech) in 100 µl of staining buffer ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed and incubated with a peroxidase-conjugated goat anti-mouse anti-IgM or anti-IgG antibody (Southern Biotech). After washing ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were washed with TBS-T 0.1% three times and incubated for one hr at room temperature with goat anti-mouse Ig horseradish peroxidase (HRP)-conjugated antibodies (Southern Biotech, anti-IgM secondary 1:1000 (cat# 1020-05) ...
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bioRxiv - Immunology 2021Quote: ... yoelii recombinant MSP-142 and AMA-1 or 10 μg/ml anti-mouse Kappa (Southern Biotech). Cells were incubated for 16-24 h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... or mouse anti-hamster-IgG2/3-HRP (Southern Biotech, Cat# 1935–05, 1:8000 in PBST) or mouse anti-hamster-IgG1-HRP (Southern Biotech ...
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bioRxiv - Microbiology 2019Quote: ... We used alkaline phosphatase-conjugated goat α-mouse IgG at 1:2000 (Southern Biotech, Birmingham, AL), and visualized using alkaline phosphatase substrate [Tris-base ...
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bioRxiv - Immunology 2021Quote: ... or mouse anti-hamster-IgG2/3-HRP (Southern Biotech, Cat# 1935-05, 1:8000 in PBST). Optical density was measured at 490 nm and 630 nm using an ELX800 plate reader (Biotek Instruments) ...
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bioRxiv - Microbiology 2021Quote: ... or mouse anti-hamster-IgG2/3-HRP (Southern Biotech, Cat# 1935-05, 1:8000 in PBST) or mouse anti-hamster-IgG1-HRP (Southern Biotech ...
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bioRxiv - Biochemistry 2023Quote: ... followed by the addition of 1/10,000 goat anti-mouse IgA-biotin (Southern Biotech, #1040-08) or 1/10,000 goat anti-IgG-biotin (Southern Biotech #1030-08 ...
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bioRxiv - Immunology 2022Quote: ... beads were incubated for 1 hour with PE-conjugated goat anti-mouse IgG Abs (Southern Biotech). After three washes ...
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bioRxiv - Genetics 2019Quote: ... washed and incubated with HRP-conjugated secondary antibody (1:10 000, Southern Biotech). Immunoblots were developed using ECL prime (GE healthcare ...
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bioRxiv - Microbiology 2022Quote: ... then incubated in 0.1% TBST containing 1:4000 goat anti-rabbit antibody conjugated to HRP for 1 h at RT (SouthernBiotech).
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bioRxiv - Immunology 2020Quote: ... anti-mouse IgG1-HRP or anti-mouse IgG2a-HRP (Southern Biotech). After incubation and washes ...
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bioRxiv - Immunology 2024Quote: ... for mouse serum or mouse-anti-monkey IgG (SouthernBiotech; RRID: AB_2796069) for NHP serum) ...
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bioRxiv - Immunology 2023Quote: ... goat anti-mouse IgG2c or goat anti-mouse IgG3 (Southern Biotech). Plates were blocked for 1 hr using PBS 1% BSA ...
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bioRxiv - Immunology 2019Quote: ... Murine antibodies were detected using alkaline phosphatase conjugated goat anti-mouse IgG or IgA (Southern Biotech, Cat #1030-04 and 1040-04). Rabbit antibodies were detected using alkaline phosphatase conjugated goat anti-rabbit IgG (Southern Biotech ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed with 0.05% Tween-20 in PBS and further incubated with HRP-conjugated goat antibodies directed against mouse IgG (Southern Biotech, Birmingham, AL) for 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... After 3 washes in TBST the membrane was incubated with 1:10000 anti-mouse Ig (Southern Biotech) in TBST containing 5% non-fat dry milk for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... Bound IgG was detected using alkaline phosphatase–conjugated goat anti–mouse kappa (Southern Biotech, diluted 1:1000) in 0.5% BSA ...
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bioRxiv - Cell Biology 2021Quote: ... Goat Anti-Rabbit IgG(H+L) Mouse/Human ads-HRP (4050-05, dilution 1:10000) (Southern Biotech); Alexa 488- ...
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bioRxiv - Microbiology 2022Quote: ... Microtiter polystyrene plates were coated with goat anti-mouse IgM at 1 μg/ml (SouthernBiotech, Birmingham, AL) and then blocked with 1% BSA blocking solution ...
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bioRxiv - Immunology 2021Quote: ... these beads were incubated for 1 hour with PE-conjugated goat anti-mouse IgG Abs (Southern Biotech). After three washes ...
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bioRxiv - Immunology 2023Quote: ... followed by probing the bound antibodies with an anti-monkey IgG horseradish peroxidase (HRP)-conjugated secondary antibody at 1:4000 dilution (Southern Biotech, Birmingham, AL) for 1hr at RT ...
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bioRxiv - Microbiology 2020Quote: ... Cell lysates and supernatants were subjected to immunoblot analysis for antibodies against cellular LAMP-1 (1:2000, SouthernBiotech, 9835-01), VSV-G (1:2000 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with FACs buffer (1% BSA, 1% Na+azide) and secondary antibody IgG-PE (Southern Biotech, Alabama, USA) was added in a 1:200 dilution ...
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bioRxiv - Microbiology 2023Quote: ... formaldehyde fixed parasites were adhered to glass slides and blocked with 50% FCS in PBS for 45 min before staining with primary anti-VSG (1:10,000) and secondary Alexa Fluor (1:1,000) antibodies and mounting with DAPI Fluoromount-G® (Southern Biotech). Imaging was performed with an Axioscope 2 fluorescence microscope (Zeiss ...