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Citations for SouthernBiotech :
451 - 500 of 603 citations for Mouse Alpha ketoglutarate dependent dioxygenase FTO FTO ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... samples were diluted at 1:50 and antigen-coupled microspheres were incubated with secondary antibody diluted at 1:100 each: mouse anti-human IgG1 hinge (0.1 mg/ mL, clone 4E3, SouthernBiotech); IgG2 Fc (0.1 mg/ mL ...
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bioRxiv - Microbiology 2020Quote: ... Plates were again washed with PBST and coated with 50 μL 1:10,000 diluted secondary antibody goat anti-mouse IgG HRP conjugated (1030-05, SouthernBiotech) and incubated 1 hr at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... dilution 1:10,000) and Goat Anti-Mouse IgG (H + L) Human ads-HRP (1031-05, dilution 1:10,000) (Southern Biotech); sheep anti-TGN38 (AHP499G ...
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bioRxiv - Immunology 2022Quote: ... Plates were then washed three times with PBS-T and secondary goat anti-mouse IgG-HRP antibody (Southern Biotech), diluted 1:4000 in ELISA buffer ...
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bioRxiv - Immunology 2021Quote: ... For the three-step protocol sections were stained using a goat anti-mouse IgG1-HRP-conjugated antibody (Southern Biotech) for CXCL12 and goat anti-mouse IgG2b-HRP-conjugated antibody (Southern Biotech ...
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bioRxiv - Immunology 2020Quote: ... HRP-conjugated secondary antibodies were used as follows: goat anti-mouse IgG-HRP (SouthernBiotech, Cat: 1030-05; 1:10,000) or goat anti-mouse IgA-HRP (SouthernBiotech ...
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bioRxiv - Immunology 2019Quote: ... HIV Env-specific IgG was then detected with phycoerythrin (PE)-conjugated mouse anti-human IgG (Southern Biotech, Birmingham, AL) at 2 μg/ml ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then surface stained with mouse anti-chicken Bu-1-FITC (Clone AV20; Southern Biotech, Birmingham, AL, USA) and mouse anti-chicken monocyte/macrophage-PE (Clone KUL01 ...
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bioRxiv - Immunology 2020Quote: ... coated with 100 µL/well of goat anti-mouse IgA (5 µg/mL Bic 0.1M, pH 9.6; Southern Biotech) were incubated with serial 3-fold dilutions of either fecal supernatant (100 µL ...
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bioRxiv - Immunology 2021Quote: ... RBC-depleted splenocytes were incubated with 1 μg/mL of goat F(ab’)2 anti-mouse Igκ-biotin (SouthernBiotech) for 30 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... the plates were incubated with a 1:2,000 dilution of a goat anti-mouse IgG2a-HRP antibody (Southern Biotech) in PBS supplemented with 1% FCS and incubated with gently shaking at RT for one hour ...
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bioRxiv - Immunology 2021Quote: ... Bound Abs were then reacted with HRP-conjugated goat anti-mouse IgG or IgM (Southern Biotech Cat.#1030-05) at 37lJ for 20 min ...
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bioRxiv - Immunology 2022Quote: ... 2M7 mAb isolated from rabbit PBMCs was detected with an HRP-conjugated polyclonal mouse anti-rabbit IgG (Southern Biotech) and all the positive controls were detected with an HRP-conjugated polyclonal goat anti-human IgG (Southern Biotech ...
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bioRxiv - Microbiology 2023Quote: ... the plates were washed with washing buffer twice before adding the HRP-conjugated mouse anti-monkey IgG (Southern Biotech) and incubating at room temperature for 1 hour ...
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bioRxiv - Biophysics 2023Quote: ... at a 1:250 dilution and protein was detected with a HRP-conjugated goat anti-mouse antibody (Southern Biotech) using a 1:5000 dilution ...
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bioRxiv - Immunology 2022Quote: ... cells secreting anti-NP antibody (ASC) were visualized with HRP-conjugated anti-mouse IgM or IgG3 antibodies (Southern Biotech) followed by AEC staining Kit (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were stained again for 30 min with 0.5 µg of FITC-conjugated anti-mouse IgA antibody (Southern Biotech) in 100 µl of staining buffer to refresh and harmonize the staining between batches ...
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bioRxiv - Microbiology 2023Quote: ... 20 µl/well of a Phosphatase Alkaline-conjugated goat anti-mouse IgA (Southern Biotech, Imtec diagnostics, Ref: 1040-04), diluted 1/1000 in Dilution Buffer ...
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bioRxiv - Bioengineering 2024Quote: ... plates were washed with PBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG1 antibodies (Southern Biotech) or HRP-conjugated goat anti-mouse IgG2a-HRP antibodies (Southern Biotech ...
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bioRxiv - Bioengineering 2024Quote: ... Secondary antibody was horseradish peroxidase (HRP) conjugated anti-mouse IgG antibody and was procured from Southern Biotech (Birmingham AL). KPL TMB Stop Solution was purchased from Sera Care (Milford ...
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bioRxiv - Immunology 2024Quote: ... with serum diluted 1:10 followed by an incubation with goat anti-mouse IgG-FITC (1:100; Southern Biotech). Images were acquired on an inverted fluorescence microscope (Olympus) ...
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bioRxiv - Immunology 2023Quote: ... The IgG assay contained a goat anti-mouse IgG Fc antibody (SouthernBiotech, 1033-31, labeled with Alexa Fluor 488) and the IgM assay a donkey anti-mouse IgM F(ab’)2 (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2020Quote: ... The assay was performed as described above except for the secondary antibodies (1:2,000. goat Anti-Mouse IgG2a cross absorbed HRP antibody, Southern biotech, 1083-05 ...
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bioRxiv - Immunology 2021Quote: ... Wells were then washed 3 times with 200µL PBST before incubation for 1 hour with secondary antibodies (HRP-labeled Goat anti-mouse IgG1/IgG2b/IgG2c purchased from SouthernBiotech or HRP-labeled Goat anti-mouse IgG Fcγ purchased from Jackson ImmunoResearch ...
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bioRxiv - Immunology 2021Quote: ... then incubated with horseradish peroxidase (HRP)-conjugated mouse anti-monkey IgG (catalog number 4700-05, Southern Biotech, Birmingham, AL, USA) for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... and incubated with either rabbit anti-mouse IgG-HRP (Jackson Immuno) or goat anti-hamster IgG(H+L)-HRP (SouthernBiotech) diluted in casein for mouse or hamster samples ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed 3X with PBS and 3X with PBS-T and then anti-mouse IgG/IgA-biotin (Southern Biotech) diluted 1:1000 in PBS-T was added and incubated overnight at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed 3x and incubated with a 1:50,000 dilution of anti-mouse total IgG-HRP antibody (Southern Biotech) and incubated for 1h at 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... and then for 1 hour at room temperature with a 1:4000 diluted rabbit anti-mouse HRP secondary antibody (SouthernBiotech Cat# 6170-05 ...
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bioRxiv - Immunology 2021Quote: ... at 1×106 cells/well and stimulated for 48 hours with 5 μg/mL of RBD-His or hCD81-His or media alone (RPMI + 5% FCS) in the presence of anti-mouse CD28 antibody [0,5 μg/ml] (Southern Biotech). As positive control cells were stimulated with anti-mouse CD3 [0,05 μg/ml] (Southern Biotech) ...
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bioRxiv - Immunology 2020Quote: ... The membrane was washed in PBS containing Tween-20 (0.05%) and was incubated for 1 h with horseradish peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (Southern Biotech) diluted 1:20,000 accordingly ...
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bioRxiv - Immunology 2020Quote: ... Antibody was detected using an HRP-conjugated Goat anti-Mouse IgG kappa secondary antibody (Southern Biotech, PA1-86015, 1:1000) and developed with 1-Step Turbo TMB ELISA Substrate (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were stimulated with buffer (1% FBS/HBSS) or 10 μg/mL goat F(ab’)2 anti-mouse Igκ (SouthernBiotech) and Indo-1 fluorescence (violet vs blue ...
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bioRxiv - Immunology 2020Quote: ... washed thrice with PBST and detected with HRP-conjugated mouse anti-human IgG (Fc only) (Southern Biotech, Birmingham, AL, USA) antibody at a 1:2000 dilution ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 5 times with PBS-T and incubated with goat anti-mouse IgG1 antibody conjugated with horseradish peroxidase (SouthernBiotech) for 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Bound IgM monoclonal antibody was detected with PE-labelled goat anti-mouse IgM secondary antibody (Southern Biotech, Birmingham, AL, US) (Halpin et al. ...
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bioRxiv - Immunology 2022Quote: ... A volume of 25 μL of a 1:1,000 dilution of Mouse Anti-Biotin-AP (Southern Biotech, cat. #6404-04) in blocking buffer was added to each of the wells of the HN plates and incubated for 1 hr at RT ...
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bioRxiv - Microbiology 2022Quote: ... the plate was incubated in goat anti-mouse horseradish peroxidase (GAM-HRP) at a 1:5000 ratio (SouthernBiotech, Birmingham, AL). Lastly ...
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bioRxiv - Immunology 2022Quote: ... The bound rabbit plasma IgG was detected with a horseradish peroxidase (HRP)-conjugated polyclonal mouse anti-rabbit IgG (Southern Biotech) and the CytoGam was detected with an HRP-conjugated polyclonal goat anti-human IgG (Southern Biotech ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed and the samples were incubated with 50 μl of streptavidin-HRP diluted in blocking solution and standards with 50 μl of 0.175 μg/ml rat anti-mouse IgE-HRP (1130-05, Southern Biotech) diluted in blocking solution for 1 hour at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... the arrays were incubated with a mixture of goat anti-mouse IgM HRP (10 µg/mL; #1021-05 Southern Biotech) and donkey anti-goat antibody labeled with AlexaFluor555 (5 µg/mL ...
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bioRxiv - Microbiology 2023Quote: ... Bound antibodies were detected by using goat anti-mouse IgG conjugated to horseradish peroxidase (HRP; Southern Biotech, Birmingham, AL, USA) diluted 1:2000 in PBST and developed using 3,3’,5,5’-tetramethylbenzidine substrate (Thermo Scientific Pierce ...
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bioRxiv - Immunology 2023Quote: ... 25 μl of undiluted supernatant was added to wells coated with 1 μg/ml goat anti-mouse Ig (Southern Biotech) or HAPR8 and for serum samples 3-fold serial dilutions of serum samples starting at 1/100 were added to wells coated with NP4-BSA (10 μg/ml ...
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bioRxiv - Microbiology 2023Quote: ... was used except that plates were coated with anti-human kappa and lambda light chain specific mouse antibodies (Southern Biotech) at 1:1 ratio diluted to 1 μg/ml in PBS overnight ...
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bioRxiv - Microbiology 2023Quote: ... bound S8V1-157 was detected by incubating the microspheres with 2 μg/ml PE-conjugated rat anti-mouse IgG1 (SB77e, SouthernBiotech) for 1 hr at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 × 105 cells in 100 μL per well were plated in a 96-well plate pre-coated with anti-mouse CD3ε (1 μg/mL, SouthernBiotech). Tumors were harvested from mice 10 days post inoculation and dissociated using gentleMACS (Miltenyi Biotec) ...
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bioRxiv - Biochemistry 2024Quote: ... PVDF membranes containing blotted proteins were washed in excess PBS-Tween 20 for 1h at room temperature before application of a 1:2000 dilution of primary mouse Anti-Human IgA1 antibodies conjugated to biotin (SouthernBiotech). Blots were washed 10 min 3 x in PBS followed by treatment with a 1:2000 dilution of ExtrAvidin®−Peroxidase conjugate (Sigma ...
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bioRxiv - Immunology 2024Quote: ... Membranes were incubated in TBST containing 5% (w/v) skimmed milk with goat anti-mouse IgG conjugated to horseradish peroxidase (HRP) (1:2000, 1010-05, SouthernBiotech) for 3h at 4°C in a rotary platform and washed thrice with TBST for 10 min each time ...
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bioRxiv - Immunology 2024Quote: ... wells were washed six times with PBS supplemented with 0.1 % Tween20 and incubated for 2 h with 1 μg/mL biotinylated goat α-mouse IgM or IgG1 (SouthernBiotech) at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... and probed using the indicated primary antibodies (Table SX) and anti-mouse or anti-rabbit IgG-linked HRP secondary antibodies (Southern Biotech) followed by ECL (Luminata Crescendo or Forte ...