Labshake search
Citations for SouthernBiotech :
1 - 50 of 868 citations for Integrin beta 1 binding protein 1 ITGB1BP1 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... biotin-conjugated secondary antibodies (1:200, SouthernBiotech) and avidin-biotin-peroxidase complex (Vectastain Elite ...
-
bioRxiv - Cell Biology 2022Quote: ... Antibody binding was visualized with peroxidase-conjugated sheep anti-rabbit immunoglobulin (Southern Biotech; 4010-05; 1:15000), or sheep anti-mouse immunoglobulin (GE Healthcare ...
-
bioRxiv - Cell Biology 2022Quote: ... Antibody binding was visualized with peroxidase-conjugated sheep anti-rabbit immunoglobulin (Southern Biotech; 4010-05; 1:15000), sheep anti-mouse immunoglobulin (GE Healthcare ...
-
bioRxiv - Neuroscience 2022Quote: ... The next day slides were washed and incubated for 1 hr in a secondary biotin-conjugated anti-mouse antibody (1:200, goat anti mouse, SouthernBiotech, Birmingham, AL) mixed in blocking solution ...
-
bioRxiv - Microbiology 2020Quote: ... Antibody binding to the spike proteins was determined using a 1:2000 diluted HRP conjugated goat anti-human IgG (ITK Southern Biotech) for 1 h at RT and tetramethylbenzidine substrate (BioFX) ...
-
bioRxiv - Immunology 2022Quote: ... Goat anti-mouse IgA biotin (Southern Biotech, 1: 5,000; AL, USA). Streptavidin-HRP (Vector Laboratories ...
-
bioRxiv - Biochemistry 2023Quote: ... or 1/10,000 goat anti-IgG-biotin (Southern Biotech #1030-08) diluted in PBS-BSA 1 % (100 μL/well) ...
-
bioRxiv - Biochemistry 2024Quote: ... PVDF membranes containing blotted proteins were washed in excess PBS-Tween 20 for 1h at room temperature before application of a 1:2000 dilution of primary mouse Anti-Human IgA1 antibodies conjugated to biotin (SouthernBiotech). Blots were washed 10 min 3 x in PBS followed by treatment with a 1:2000 dilution of ExtrAvidin®−Peroxidase conjugate (Sigma ...
-
bioRxiv - Cancer Biology 2022Quote: ... Plates were washed with PBS + 0.05% Tween20 and binding was detected with anti-kappa HRP antibody (1:5000, Southern Biotech #2060-05) and developed with the TMB substrate (KPL (Mandel ...
-
bioRxiv - Physiology 2021Quote: ... A goat anti-mouse IgG- biotin antibody (Southern Biotech) was diluted 1:5000 in reagent diluent and added to all wells ...
-
bioRxiv - Immunology 2022Quote: ... while biotin-labeled goat anti-mouse IgA Ab (1:5000, Southern Biotech, Cat#1040-08) plus HRP-conjugated streptavidin (1:7000 ...
-
bioRxiv - Immunology 2023Quote: ... IgG3 was measured following 6 washes and a 1-h incubation with 100 μL/well 1:5,000 mouse anti-human biotin-conjugated IgG3 hinge (SouthernBiotech, Birmingham, Alabama, USA) then 6 washes and 1-h incubation with 100 μL/well 1:40,000 HRP-conjugated streptavidin (Jackson ImmunoResearch Labs) ...
-
bioRxiv - Biochemistry 2023Quote: ... followed by the addition of 1/10,000 goat anti-mouse IgA-biotin (Southern Biotech, #1040-08) or 1/10,000 goat anti-IgG-biotin (Southern Biotech #1030-08 ...
-
bioRxiv - Immunology 2021Quote: ... Binding of primary antibodies was then measured via HRP-tagged antibodies specific for the relevant species (SouthernBiotech) and visualized by chemiluminescent substrate (Immobilon Classico/ Forte western HRP substrate or Thermo Fisher SuperSignal West Dura Extended Duration Substrate).
-
bioRxiv - Genetics 2021Quote: ... Wells were washed as described and biotin conjugated goat anti-human IgG (Jackson AffiniPure #109-065-098; 1:30,000 or Southern Biotech 2049-08; 1:1,000) in PBS/1.0% BSA detection antibody was added to the wells at 100ul/well and incubated for 2 hours at room temperature with shaking ...
-
bioRxiv - Immunology 2024Quote: ... Antibody binding was detected using PE-conjugated goat-anti-mouse IgG secondary antibody (2 μg/mL, Southern Biotech). Results were acquired on a Bio-Plex 200 system (Bio-Rad ...
-
bioRxiv - Biophysics 2023Quote: ... at a 1:250 dilution and protein was detected with a HRP-conjugated goat anti-mouse antibody (Southern Biotech) using a 1:5000 dilution ...
-
bioRxiv - Immunology 2020Quote: ... anti-NIP antibody binding was developed with the SBA Clonotyping System-HRP (Southern Biotech). Absorbance at 405 nm was determined in a FilterMax F5 microplate reader (Molecular Devices) ...
-
bioRxiv - Molecular Biology 2022Quote: ... The following antibodies were used: collagen 1 (1:1000, Southern Biotech, 1310), collagen 3 (1:500 ...
-
bioRxiv - Neuroscience 2021Quote: ... samples were labelled with biotin-coupled antibodies (all antibodies were purchased from Southern Biotech: goat anti mouse (1012-08), goat anti rat (3052-08) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Primary antibody binding was detected with rabbit anti-mouse IgG1-APC (Southern Biotech, Birmingham, USA). To assess binding of recombinant CXCL12γ ...
-
bioRxiv - Genetics 2022Quote: ... 96-well flat-bottom high-binding plates were coated with anti-Ig antibodies (Southern Biotech) diluted in carbonate buffer for each antibody subtype measured ...
-
bioRxiv - Immunology 2021Quote: ... RBC-depleted splenocytes were incubated with 1 μg/mL of goat F(ab’)2 anti-mouse Igκ-biotin (SouthernBiotech) for 30 minutes at 4°C ...
-
bioRxiv - Immunology 2022Quote: ... A volume of 25 μL of a 1:1,000 dilution of Mouse Anti-Biotin-AP (Southern Biotech, cat. #6404-04) in blocking buffer was added to each of the wells of the HN plates and incubated for 1 hr at RT ...
-
bioRxiv - Physiology 2023Quote: ... Non-specific antibody binding was blocked for 1 hour at room temperature with a blocking solution containing: 5% normal goat serum (006001, SouthernBiotech™), 0.3% Triton™ X-100 (X100 ...
-
bioRxiv - Immunology 2024Quote: ... Specific IgG was detected for 1 hour at room temperature with biotin rat anti-mouse IgG (Southern Biotech, Birmingham, AL, USA) biotin rat anti-mouse IgG1 (Southern Biotech) ...
-
bioRxiv - Bioengineering 2022Quote: ... Secreted IgG was detected by binding to HRP-conjugated goat anti-human IgG secondary antibody (Southern Biotech). Spots were developed with AEC Substrate Kit ...
-
bioRxiv - Immunology 2024Quote: ... IgA ELISAs: high-binding 96-well ELISA plates were coated using unlabelled IgA coating antibodies (Southern Biotech). Following wash and blocking steps ...
-
bioRxiv - Cell Biology 2021Quote: ... Secondary antibodies conjugated to HRP (Southern Biotech; 1:10,000) were used for the chemiluminescent detection.
-
bioRxiv - Genetics 2021Quote: ... Primary antibodies (1:250 Anti-Fibronectin-1, F3648, Sigma-Aldrich; 1:100 Goat Anti-Type I Collagen, 1310-01, Southern Biotech; 1:100 Goat Anti-Type V Collagen ...
-
bioRxiv - Immunology 2020Quote: ... anti-IgG1-biotin (Southern Biotech) and anti-IgG2a-PE ...
-
bioRxiv - Microbiology 2022Quote: ... Slides were exposed to biotin labelled goat Fab anti-human IgG secondary antibody (Southern Biotech, Birmingham, AL) at a 1:2500 dilution for 1 h at room temperature ...
-
bioRxiv - Microbiology 2020Quote: ... Antibody binding to the peptides was determined using a goat anti-human IgG HRP conjugate (ITK Southern Biotech) for 1 h at RT and tetramethylbenzidine substrate (BioFX) ...
-
bioRxiv - Cell Biology 2021Quote: ... HRP-conjugated secondary antibodies (1/5000) were from Southern Biotech. Alexa-conjugated secondary antibodies were from Life Technologies.
-
bioRxiv - Immunology 2022Quote: ... the plates were incubated with serially diluted serum samples and then with biotin-conjugated detection antibodies (Southern Biotech) followed by streptavidin-conjugated HRP (Jackson Immunoresearch) ...
-
bioRxiv - Immunology 2020Quote: ... CMV glycoprotein–specific antibody binding was detected with phycoerythrin-conjugated goat anti-human IgG (2 μg/mL, Southern Biotech). Beads were washed and acquired on a Bio-Plex 200 instrument (Bio-Rad) ...
-
bioRxiv - Neuroscience 2023Quote: ... and goat anti-collagen IV antibody (1:200, Southern Biotech, US) for 24 hours at 4°C ...
-
bioRxiv - Microbiology 2022Quote: ... then incubated in 0.1% TBST containing 1:4000 goat anti-rabbit antibody conjugated to HRP for 1 h at RT (SouthernBiotech).
-
bioRxiv - Immunology 2024Quote: ... biotin rat anti-mouse IgG1 (Southern Biotech), or biotin rat anti-mouse IgG2b (Southern Biotech) ...
-
bioRxiv - Microbiology 2020Quote: ... Cell lysates and supernatants were subjected to immunoblot analysis for antibodies against cellular LAMP-1 (1:2000, SouthernBiotech, 9835-01), VSV-G (1:2000 ...
-
bioRxiv - Bioengineering 2021Quote: ... and then for 1 hour at room temperature with a 1:4000 diluted rabbit anti-mouse HRP secondary antibody (SouthernBiotech Cat# 6170-05 ...
-
bioRxiv - Immunology 2021Quote: ... Cells were washed with FACs buffer (1% BSA, 1% Na+azide) and secondary antibody IgG-PE (Southern Biotech, Alabama, USA) was added in a 1:200 dilution ...
-
bioRxiv - Microbiology 2023Quote: ... formaldehyde fixed parasites were adhered to glass slides and blocked with 50% FCS in PBS for 45 min before staining with primary anti-VSG (1:10,000) and secondary Alexa Fluor (1:1,000) antibodies and mounting with DAPI Fluoromount-G® (Southern Biotech). Imaging was performed with an Axioscope 2 fluorescence microscope (Zeiss ...
-
bioRxiv - Microbiology 2023Quote: ... formaldehyde fixed parasites were adhered to glass slides and blocked with 50% FCS in PBS for 45 min before staining with primary anti-VSG (1:10,000) and secondary Alexa Fluor (1:1,000) antibodies and mounting with DAPI Fluoromount-G® (Southern Biotech). Imaging was performed with an Axioscope 2 widefield fluorescence microscope (Zeiss ...
-
bioRxiv - Immunology 2021Quote: ... 1:300 for IgA.] HRP conjugated antibodies were all from Southern BioTech: IgM (#1021-05) ...
-
bioRxiv - Neuroscience 2023Quote: ... USA) and Goat anti-collagen IV antibody (1:200, Southern Biotech, US), mouse anti Human Protein Gene Product 9.5 (PGP9.5 ...
-
bioRxiv - Microbiology 2020Quote: ... Antibody binding was detected using directly labeled IgG or Alexa Fluor647-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech). Samples were measured using flow cytometry (BD FACSVerse ...
-
bioRxiv - Immunology 2024Quote: ... AP-conjugated goat anti-mouse immunoglobulin antibodies were added to detect IgM (1 in 2000) or IgG (1 in 1000) (Southern Biotech). Signal was detected with Sigma FAST P-nitrophenyl phosphate (pNPP ...
-
bioRxiv - Immunology 2024Quote: ... Horseradish peroxidase-labelled goat anti-human IgG (IgG-HRP, diluted 1:5000) or IgA (IgA-HRP, diluted 1:2000) secondary antibodies (Southern Biotech) were added at 50 µL/well for one hour at 37°C ...
-
bioRxiv - Bioengineering 2024Quote: ... or a 1:1 mixture of anti-mouse kappa and anti-mouse lambda capture antibodies at 5 µg/mL (for standards; Southern Biotech) in PBS at 4 °C overnight ...