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Citations for SouthernBiotech :
401 - 450 of 618 citations for IL 4 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... the sections were mounted on glass slides with 4’,6-diamidino-2-306 phenylindole (DAPI) Fluoromount-G (Southern Biotech) or Vectashield (Vector Laboratories ...
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The antibiotic bedaquiline activates host macrophage innate immune resistance to bacterial infectionbioRxiv - Immunology 2019Quote: ... coverslips were set in Fluoromount G medium containing 1 µg/ml 4′,6-diamidino-2-phenylindole (DAPI) (SouthernBiotech, Birmingham, Alabama) on microscope slides ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were fixed with 4% paraformaldehyde for 30 min at room temperature and stained with DAPI Fluoromount-G (Southern Biotech) to observe intracellular localization using an Olympus BX63 fluorescence microscope ...
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bioRxiv - Cancer Biology 2023Quote: ... the coverslips were fixed in 4% paraformaldehyde for 20 min at RT and mounted in DAPI Fluoromount-G (SouthernBiotech, USA). Slides were subjected to confocal imaging in a Zeiss LSM 700 microscope (Zeiss ...
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bioRxiv - Neuroscience 2023Quote: ... Coverslips were washed with PBS and mounted with Fluoromount-G with DAPI (4’,6’-diamidino-2-phenylindole dihydrochloride) (cat. no. 0100-20, SouthernBiotech) for nuclear staining ...
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bioRxiv - Microbiology 2020Quote: ... and then incubated with 1:100000 secondary antibody Anti-Mouse Kappa conjugated to Horseradish Peroxidase (Southern Biotech) for 2 h before washing again ...
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bioRxiv - Immunology 2021Quote: ... After 3 washes in TBST the membrane was incubated with 1:10000 anti-mouse Ig (Southern Biotech) in TBST containing 5% non-fat dry milk for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... Bound IgG was detected using alkaline phosphatase–conjugated goat anti–mouse kappa (Southern Biotech, diluted 1:1000) in 0.5% BSA ...
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bioRxiv - Microbiology 2020Quote: ... Antibody concentration was determined by comparing absorbance values to known concentrations of mouse IgG (0107-01, SouthernBiotech) bound to the plate with goat anti-mouse Ig (1010-01 ...
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bioRxiv - Cell Biology 2021Quote: ... Goat Anti-Rabbit IgG(H+L) Mouse/Human ads-HRP (4050-05, dilution 1:10000) (Southern Biotech); Alexa 488- ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated goat-anti-mouse isotype-specific (IgM, IgG) antibodies (50 μL at 0.1 μg/mL; Southern Biotech) were added and incubated at RT for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... Bound serum antibodies were detected with polyclonal biotinylated goat anti-mouse IgM or anti-IgG1 (Southern Biotech), streptavidin-alkaline phosphatase conjugate (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Microtiter polystyrene plates were coated with goat anti-mouse IgM at 1 μg/ml (SouthernBiotech, Birmingham, AL) and then blocked with 1% BSA blocking solution ...
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bioRxiv - Immunology 2019Quote: ... Blots were washed and bands were detected with anti-mouse IgG Fc-HRP (Southern Biotech; 1030-05) at 1:10,000 for 1 hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... The cells were stained for surface expression of IgA using PE-conjugated anti-mouse IgA antibody (SouthernBiotech) and sorted by a FACS Aria IIIu and influx sorter (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... these beads were incubated for 1 hour with PE-conjugated goat anti-mouse IgG Abs (Southern Biotech). After three washes ...
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bioRxiv - Immunology 2021Quote: ... For PBS suspensions of feces and mouse IgA and IgG (Southern Biotech, Cat#0106-01; RRID: AB_2714214), 5 × 105 ler/lux-expressing C ...
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bioRxiv - Immunology 2020Quote: ... HRP-conjugated goat anti-mouse IgG second antibody (catalog number 1030-05, Southern Biotech, Birmingham, AL, USA) was added ...
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bioRxiv - Microbiology 2021Quote: ... Plates were then washed 5 times and incubated with goat anti-mouse Ig conjugated to HRP (SouthernBiotech) for 1 hour at room temp ...
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bioRxiv - Molecular Biology 2021Quote: ... IgM was immunoprecipitated from lysates or media samples with a goat anti-mouse IgM antibody (Southern Biotech). Immunoprecipitates were washed with lysis buffer and eluted from beads with reducing SDS-PAGE sample buffer and analyzed by SDS-PAGE ...
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bioRxiv - Immunology 2023Quote: ... alkaline phosphatase-conjugated goat anti-mouse IgG1 or IgM (Southern Biotech, cat: 1071-04, cat: 1021-04) detection antibody was added (1:2000 ...
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bioRxiv - Immunology 2023Quote: ... Secondary antibody (Goat Anti-Mouse IgG-HRP or IgA-HRP [SouthernBiotech #1030-05 and #1040-05, respectively]) was added at a 1:1000 dilution in PBS with 0.1% Tween and 5% FBS overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... and biotin-labeled goat anti-mouse IgA (Cat# 1040-08) were obtained from Southern Biotech (Birmingham, AL). HRP-conjugated anti-mouse IgG Fab (Cat# A9917 ...
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bioRxiv - Microbiology 2023Quote: ... Sample homogeneous solution and standards (mouse IgA Isotype Control UNLB, Southern Biotech, Imtec Diagnostics, Ref: 0106-01) were diluted in Dilution Buffer (15 mM Trizma-Acetate ...
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bioRxiv - Immunology 2023Quote: ... then incubated with an HRP-conjugated human/bovine/horse cross-adsorbed anti-mouse secondary antibody (Southern Biotech) at a 1:20,000 dilution for 1 hour at RT ...
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bioRxiv - Microbiology 2024Quote: ... The following secondary antibodies were used: rabbit-anti mouse IgG-horseradish peroxidase (HRP; SouthernBiotech; dilution 1:10‘000) and goat-anti rabbit IgG-HRP (SouthernBiotech ...
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bioRxiv - Cell Biology 2024Quote: ... or HRP-conjugated α-mouse IgM (Southern Biotech, 1020-05, 1:5000 in 1 % BSA in PBS) for 4 h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... we used the following secondary antibodies during ELISA: goat anti-mouse IgG1 HRP antibody (#1071-05, SouthernBiotech), goat anti-mouse IgG2a HRP antibody (#1081-05 ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were stored at 4°C until mounting on non-gelatin-coated slides followed by cover of DAPI fluoromount (Southern Biotech) and cover-slipped ...
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bioRxiv - Immunology 2020Quote: ... plates were washed 4 times with PBS-T and an anti-monkey IgG conjugated to horseradish peroxidase (Southern Biotech 4700-5) 1 hour at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) with the use of DAPI Fluoromount-G (0100-20; Southern Biotech). Sections were also stained for β-galactosidase (β-gal ...
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bioRxiv - Microbiology 2020Quote: ... Guts were washed 5 times in PBS-T and then mounted on slides in Flouromount plus 4’,6-diamidino-2-phenylindole nuclear stain (DAPI, Southern Biotech) and sealed with a cover slip ...
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bioRxiv - Biochemistry 2022Quote: ... labeled protein was visualized using the AP substrate 5-bromo-4-chloro-indolyl-phosphate/nitro blue tetrazolium (BCIP/NBT; Southern Biotech).
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bioRxiv - Immunology 2023Quote: ... Sections were washed in PBS before incubation with secondary antibodies or labelled streptavidin (Supplementary Table 4) for 1hr at room temperature followed by mounting with DAPI containing fluoromount (Southern Biotech). Small to medium sized airways in lung sections were captured by an investigator blind to sample identity with an EVOS FL imaging system (Thermo) ...
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bioRxiv - Microbiology 2023Quote: ... 4°C) and incubated with goat anti-human IgG-Alexa Fluor 647 and goat anti-human IgM-PE (both Southern Biotech) for 30 minutes at 4°C at 600 rpm ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed again and the nuclei were stained with DAPI (4’,6-diamidino-2-phenylindole) before mounting with Fluoromount-G (Southern Biotech). Primary antibodies against histone H3 methylation on lysine 4 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the embryos were stained with 10nM DAPI in 70% glycerol in 1X PBS overnight at 4°C protected from light, washed in 1X SSCT (10 min/wash, twice) before mounting with Fluoromount G (Southern Biotech) on glass bottom dishes (Cellvis ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies used include the following: Goat Anti-Mouse IgG (H+L; Southern Biotech, 1031-05, 1:2,000) and Goat Anti-Rabbit IgG (Life Technologies ...
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bioRxiv - Immunology 2019Quote: ... The plates were then washed and incubated with peroxidase-conjugated goat anti-mouse IgM Fc-specific (Southern Biotech). In differentiation assays ...
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bioRxiv - Biophysics 2020Quote: The anti-mouse-Igκ antibody for clustering BCRs was purchased from Southern Biotech (Birmingham, AL; catalogue #1050-01). AF647-conjugated anti-mouse-IgM Fab fragments (catalogue #115-607-020 ...
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bioRxiv - Biochemistry 2021Quote: ... 500 μL of a secondary antibody solution (5 μM Alexa Fluor® 488 anti-mouse (SouthernBiotech; 1031-30) and 10 μM Alexa Fluor® 647 anti-human (SouthernBiotech ...
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bioRxiv - Microbiology 2021Quote: ... incubated with a horseradish peroxidase-conjugated goat anti-mouse IgG secondary antibody (SouthernBiotech, 1030-05, 1:2000 dilution) at RT for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... followed by addition of 50 µL of 1:1,000 anti-mouse IgG-HRP (Southern Biotech Cat. #1030-05) in PBST or 1:1000 of anti-mouse IgA-HRP in PBSTBA (SouthernBiotech) ...
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bioRxiv - Immunology 2021Quote: ... HRP-conjugated mouse anti-histidine-tag IgG (1:4000) (catalog number 4603-05, Southern Biotech, Birmingham, AL, USA) was added for 1 hour followed by addition of TMB substrate ...
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bioRxiv - Immunology 2020Quote: ... Detection was performed using an HRP-linked goat anti-rabbit or goat anti-mouse secondary antibody (Southern Biotech) and chemiluminescence detection (ECL Plus™ ...
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bioRxiv - Immunology 2020Quote: ... plates were incubated with anti-mouse IgG secondary Ab (Southern Biotech, cat no 1170-05, dilution 1:1000) 1hr at RT ...
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bioRxiv - Bioengineering 2021Quote: ... and Alexa Fluor 488-conjugated goat anti-mouse IgM (1:250 dilution, 1020-30, Southern Biotech, Birmingham, AL) for 30 minutes at 4°C and analyzed in a BD FACSCanto II (BD Biosciences ...
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bioRxiv - Molecular Biology 2020Quote: ... the following secondary antibodies were used diluted 1:1000 in 5%-milk: anti-mouse (Southern Biotech, 1031–05) and a 1:1 mix of anti-rabbit (Southern Biotech ...
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bioRxiv - Immunology 2020Quote: ... or with goat anti-mouse IgM-HRP (Southern Biotech #1021-05, 10 mL, 1:5,000, 1 h, RT) for detection of M86 mAb ...
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bioRxiv - Immunology 2020Quote: ... were coated with either αGal-BSA (Dextra) or goat anti-mouse Ig(H+L) (Southern Biotech #1010-01) (5 µg/mL in Carbonate-Bicarbonate buffer ...