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Citations for SouthernBiotech :
251 - 300 of 422 citations for Human Complexin 2 CPLX2 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Plates were washed three times and incubated with HRP-conjugated goat anti-human secondary antibody (ITK Southern Biotech) diluted 1:2000 in blocking buffer for 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... and then incubated with either goat anti-human IgG-HRP conjugated antibodies (Southern Biotech, catalog no. 2040-05), goat anti-human IgA-HRP conjugated antibodies (Southern Biotech ...
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bioRxiv - Immunology 2020Quote: ... was coated with a 1:500 dilution of unlabeled goat anti-human IgG (Southern Biotech, Birmingham, AL, USA) and incubated at 37°C for 1 h ...
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bioRxiv - Immunology 2021Quote: ... Total antigen-specific IgG was then detected with a mouse anti-human IgG phycoerythrin-conjugated Ab (Southern Biotech) at 2 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... beads were incubated for 1 hr with PE-conjugated mouse anti-human IgG antibodies (JDC-10, Southern Biotech). After three washes ...
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bioRxiv - Immunology 2023Quote: ... and incubated with 0.75x serial dilutions of purified polyclonal human antibodies (IgG, SouthernBiotech, Cat # 0150-01; IgM, SouthernBiotech, Cat # 0158L-01 ...
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bioRxiv - Immunology 2022Quote: ... and all the positive controls were detected with an HRP-conjugated polyclonal goat anti-human IgG (Southern Biotech) by 1 hour incubation at room temperature ...
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bioRxiv - Immunology 2024Quote: ... 50 μl of Goat anti-Human IgG Fc Multi-species SP ads-PE (Southern Biotech, catalog:2014-09) and Goat anti-Mouse IgG Fc Human ads-PE (Southern Biotech ...
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bioRxiv - Biophysics 2021Quote: ... Both horseradish peroxidase (HRP) conjugated anti-human kappa and anti-rabbit IgG were purchased from SouthernBiotech (Birmingham, AL, USA) and Abcam (Cambridge ...
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bioRxiv - Microbiology 2021Quote: ... samples were diluted at 1:50 and antigen-coupled microspheres were incubated with secondary antibody diluted at 1:100 each: mouse anti-human IgG1 hinge (0.1 mg/ mL, clone 4E3, SouthernBiotech); IgG2 Fc (0.1 mg/ mL ...
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bioRxiv - Microbiology 2021Quote: ... but the secondary antibody used was an anti-human IgM Peroxidase-labelled antibody produced in goats (SouthernBiotech, Birmingham, USA).
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bioRxiv - Cell Biology 2021Quote: ... dilution 1:10,000) and Goat Anti-Mouse IgG (H + L) Human ads-HRP (1031-05, dilution 1:10,000) (Southern Biotech); sheep anti-TGN38 (AHP499G ...
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bioRxiv - Immunology 2019Quote: ... HIV Env-specific IgG was then detected with phycoerythrin (PE)-conjugated mouse anti-human IgG (Southern Biotech, Birmingham, AL) at 2 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... The cells were washed with FACS buffer and incubated with secondary goat anti-human IgG PE antibody (Southern Biotech) for 1 hour at 4°C ...
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bioRxiv - Immunology 2022Quote: ... The secondary antibody solution was prepared by diluting goat anti-human IgG HRP-conjugated antibodies (SouthernBiotech, cat. # 2040-05) at 1:2,000 in blocking buffer and adding 20 to 25 μL/well ...
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bioRxiv - Immunology 2023Quote: ... antibody response to individual peptides was detected with different fluorescently labeled secondary antibodies (Goat anti-human IgM-AF488, SouthernBiotech, Cat # 2020-30 ...
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bioRxiv - Microbiology 2023Quote: ... beads were incubated for 1 hour at room temperature with Phycoerythrin-conjugated goat anti-human IgG antibodies (Southern Biotech). After three washes ...
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bioRxiv - Immunology 2023Quote: ... PE conjugated goat anti-human IgG Fc secondary Ab was added at 6μg/ml (Southern Biotech, catalog: 2014-09) and incubated for 1 hour at 37°C with shaking at 700 rpm ...
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bioRxiv - Microbiology 2024Quote: ... Antibodies used for immunoblot analysis in this study were: anti-Human IgM goat polyclonal antibody (Southern Biotech #2020-01), anti-Human IgG goat polyclonal antibody (Southern Biotech #2040-01) ...
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bioRxiv - Microbiology 2022Quote: ... α-IgA (mAb 11-44-2, FITC, SouthernBiotech catalog no. 1165-02). Cells were stained with primary antibodies (1:50 dilution ...
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bioRxiv - Immunology 2022Quote: ... or anti-IgM F(ab’)2 (5 μg/mL; Southern Biotech). Cells were immediately fixed with pre-warmed paraformaldehyde (4% final concentration) ...
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bioRxiv - Bioengineering 2019Quote: ... for 2 hr and mounted with Fluoromount-G (SouthernBiotech; 0100-20). Imaging was performed on a Nikon A1Rsi confocal microscope.
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bioRxiv - Immunology 2019Quote: ... PE goat anti-mouse IgG or PE mouse anti-human IgG (both from Southern Biotech, the latter for competition assay) was added to the plates and incubated for 1 hour at room temperature with mild agitation ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 × 105 dendritic cells or B cells were incubated with different concentrations of C03 with or without goat anti-human Fc antibody (SouthernBiotech) for 48 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... Jurkat/NF-κB-GFP-hCD40 reporter cells were incubated with different concentrations of CD40 agonist antibodies with or without goat anti-human Fc antibody (SouthernBiotech) for 24 hours ...
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bioRxiv - Immunology 2022Quote: ... Plates were then washed with TBST and incubated with secondary HRP-conjugated goat anti-human IgG (Southern Biotech, Birmingham, AL) at a 1:15,000 dilution for 45 minutes at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by detection with a 1:10,000 dilution of goat anti-human IgG-HRP (Southern Biotech 2040-05, Birmingham, AL). Plates were developed for 5 minutes with TMB substrate (VWR 95059-286) ...
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bioRxiv - Immunology 2020Quote: ... MagPlex beads were washed with PBST (PBS, 0.05% Tween-20) and incubated with anti-human IgG Fc-PE (Southern Biotech). Finally ...
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bioRxiv - Immunology 2021Quote: ... the pellet was resuspended in a 50 μL mixture of PE-anti-human CD11b (Southern Biotech, 10 μL/million cells), Alexa Fluor 647-anti-human CD35 (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... bound IgG was detected by incubating with 1:1000 polyclonal goat anti human IgG-HRP (Southern Biotech, Birmingham, AL, USA) for 1h at 37°C ...
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bioRxiv - Immunology 2020Quote: ... washed thrice with PBST and detected with HRP-conjugated mouse anti-human IgG (Fc only) (Southern Biotech, Birmingham, AL, USA) antibody at a 1:2000 dilution ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed three times and incubated with horseradish peroxidase (HRP)-conjugated goat anti-human secondary antibody (ITK Southern Biotech) diluted 1:2000 in blocking buffer for 1 hour at RT ...
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bioRxiv - Immunology 2022Quote: ... plates were washed with TBS containing 0.05% Tween-20 (TBST) and resuspended in 50 μL of Goat-anti-human IgG-PE (Southern Biotech). After 2 hours of incubation on a rotator at room temperature ...
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bioRxiv - Immunology 2022Quote: ... IgG antibodies were detected using horseradish peroxidase (HRP)-conjugated goat anti-human IgG detection antibody (Southern Biotech, Birmingham, AL, USA) at a 1:4,000 dilution and colorimetric development was accomplished using 100 μL of 0.1% 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid ...
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bioRxiv - Immunology 2023Quote: ... another microarray was developed with the second detection mix: goat anti-human kappa chain Alexa555 (Southern Biotech, Ref.:2060-32) and goat anti-human lambda chain Alexa647 (Southern Biotech ...
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bioRxiv - Microbiology 2023Quote: ... was used except that plates were coated with anti-human kappa and lambda light chain specific mouse antibodies (Southern Biotech) at 1:1 ratio diluted to 1 μg/ml in PBS overnight ...
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bioRxiv - Microbiology 2024Quote: ... Antibodies used for flow cytometry analysis performed in this study were: FITC-conjugated anti-human IgM (Southern Biotech #2020-02), goat anti-human IgG-UNLB (Southern Biotech #2040-01) ...
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bioRxiv - Biochemistry 2024Quote: ... PVDF membranes containing blotted proteins were washed in excess PBS-Tween 20 for 1h at room temperature before application of a 1:2000 dilution of primary mouse Anti-Human IgA1 antibodies conjugated to biotin (SouthernBiotech). Blots were washed 10 min 3 x in PBS followed by treatment with a 1:2000 dilution of ExtrAvidin®−Peroxidase conjugate (Sigma ...
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bioRxiv - Immunology 2021Quote: ... followed by goat F(ab’)2 anti-mouse IgG1-FITC (Southern Biotech). The percentage of living HUCCT1 cells was estimated by flow cytometry ...
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bioRxiv - Immunology 2020Quote: ... Cells were stimulated with anti-mouse IgM-F(ab)2 (Southern Biotech), ionomycin (4μM ...
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bioRxiv - Microbiology 2022Quote: ... or 2 μg/ml goat anti-hu-IgM-FITC (2020-02, SouthernBiotech) for 30 minutes at 4 °C while shaking ...
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bioRxiv - Cell Biology 2023Quote: ... goat F(ab’)2 anti-mouse IgG-PE (Southern Biotech, Birmingham, AL); and anti-phospho-AKT (Ser473) ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 4X in wash buffer and the secondary antibody Goat anti-Human Ig-HRP (Southern Biotech, Cat# 2010-05), was added and incubated at 37°C for 1 hr ...
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bioRxiv - Biochemistry 2020Quote: ... and then incubation for 30 mins at room temperature with anti-human IgG HRP at 1:4000 (Southern Biotech #2040-05). 5 washes of TBST were done followed by development using 1-Step Ultra TMB-ELISA Substrate Solution (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed with water three times before applying 25 μl of secondary antibody (goat anti-human IgG Fc-Southern Biotech, 2048-04 ...
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bioRxiv - Microbiology 2022Quote: ... the beads were incubated with 1 µg/mL Alexa Fluor647-conjugated goat F(ab’)₂ anti-human kappa (Southern Biotech, 2062-31) in PBS-TH for 30 min at 4 °C with shaking ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed three times and incubated with the goat anti-human-IgG Fc secondary antibody conjugated with alkaline phosphatase (AP, Southern Biotech) at a 1:1000 dilution in blocking buffer for 1 h at room temperature ...
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bioRxiv - Systems Biology 2022Quote: ... for capture and biotinylated anti-human insulin antibody (Eli Lilly and Company) with horseradish peroxidase conjugated streptavidin (7100-05, Southern Biotech) for detection ...
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bioRxiv - Immunology 2022Quote: ... The plates were then washed with PBS containing 0.1% Tween-20 (PBS-T), and Alkaline Phosphatase-conjugated Goat Anti-Human IgG (dilution 1:500, catalog no. 2040-04) from Southern Biotech was added and incubated for 45 min at room temperature ...
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bioRxiv - Immunology 2019Quote: ... washed 6x 300μl PBST and a 1/5000 cocktail of anti-human kappa-HRP and lambda-HRP antibodies (SouthernBiotech, Birmingham, AL) added for 1 hour in assay diluent ...