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Citations for SouthernBiotech :
351 - 400 of 432 citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Bound IL-21 was subsequently detected with mouse anti-human IL-21-biotin (clone 2B2-G20, eBioscience) followed by alkaline phosphatase-conjugated streptavidin (Southern Biotech). The signals were compared to a standard curve generated with recombinant human IL-21 (R&D Systems).
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bioRxiv - Immunology 2023Quote: ... The concentration of Ig in serum supernatants was measured using a standard sandwich ELISA with anti-human Ig primary (Cat#: 2048-01, Southern Biotech) and secondary antibodies (Cat# ...
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bioRxiv - Microbiology 2024Quote: ... Concentrations were evaluated by linear regression analysis built by potting OD405 values against a standard curve generated by titration of a human IgG-unlabeled antibody (Southern Biotech).
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bioRxiv - Immunology 2023Quote: ... Beads were then washed with PBST and reacted for 30 minutes at room temperature with biotinylated goat anti-human IgG (Southern Biotech) or goat whole IgG containing anti-monkey IgA (Novus ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed again 3x 5 minutes in 1xPBS and mounted with Fluoromount-G (SouthernBiotech). Tissue or cells lacking the SOG-Y transgene were used as a negative control ...
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bioRxiv - Neuroscience 2021Quote: ... further washed 3×5 min in PBS and mounted in Fluoromount-G (SouthernBiotech, 0100-01). Images were acquired with a Zeiss LSM700 confocal laser scanning microscope and assembly was performed with ImageJ/Fiji ...
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bioRxiv - Microbiology 2022Quote: ... the beads were incubated with 1 µg/mL Alexa Fluor647-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech, 2062-31) in PBS-TH for 30 min at 4 °C with shaking ...
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bioRxiv - Bioengineering 2022Quote: ... We washed with PBS three times to remove the unbound fractions and treated the mixtures with anti-human IgG secondary antibody (SouthernBiotech #2016-05). The plate was further incubated at room temperature for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... washed 3 times in ice-cold PBSM and then bound antibody detected with goat anti-human kappa-A647 (SouthernBiotech, cat# 2060-31) After washing ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed twice in FACS buffer and stained with 50 μl/well of 1:200 dilution of R-phycoerythrin (PE)-conjugated mouse anti-human IgG Fc antibody (SouthernBiotech #9040-09) and 1:1000 dilution of Zombie-NIR viability dye (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... the cells were stained with 50μl/well of 1:200 diluted R-phycoerythrin (PE)-conjugated mouse anti-human IgG Fc antibody (SouthernBiotech cat.# 9040-09) and 1:1000 dilution of Zombie-NIR viability dye (BioLegend cat.# 423105 ...
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bioRxiv - Immunology 2023Quote: ... IgG3 was measured following 6 washes and a 1-h incubation with 100 μL/well 1:5,000 mouse anti-human biotin-conjugated IgG3 hinge (SouthernBiotech, Birmingham, Alabama, USA) then 6 washes and 1-h incubation with 100 μL/well 1:40,000 HRP-conjugated streptavidin (Jackson ImmunoResearch Labs) ...
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bioRxiv - Immunology 2023Quote: ... MAB273 was detected by adding a 1:5,000 dilution of monkey cross-adsorbed polyclonal goat anti-human IgG HRP antibody (Southern Biotech, 2049-05) and the signal was developed as described above.
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bioRxiv - Immunology 2021Quote: ... Cells were washed and incubated for 5 min in ice with streptavidin (60 μg/ml, SouthernBiotech) for cross-linking and incubated at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were rinsed three times (5 minutes each) and coverslipped using mounting medium containing DAPI (SouthernBiotech). Images were taken using a Leica TCS SP8 confocal microscope.
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bioRxiv - Cell Biology 2020Quote: ... for 5 min for nuclei staining and coverslipped using Fluoromount G (Southern Biotech, Birmingham, AL, USA). For hyaluronic acid staining ...
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bioRxiv - Immunology 2021Quote: ... Wells were washed 5 times and secondary goat anti-IgM-HRP (Southern Biotech, cat# 1021-08) at 1:5000 dilution was incubated for 1 hr in the wells ...
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bioRxiv - Neuroscience 2023Quote: ... The slides were washed 3 × 5 mins and mounted with Fluoromount-G (SouthernBiotech, Cat # 0100-01) on microscope slides with Secure-Seal spacer (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Slides were washed 1X PBS before mounting with 5 µL DAPI Fluoromount-G® (Southern Biotech) and imaging ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 5 times with PBS/0.05% Tween20 and IgG antibodies were detected using horseradish peroxidase (HRP)-conjugated goat anti-human IgG detection antibody (Southern Biotech, Birmingham, AL, USA) at a 1:4,000 dilution and incubated for 90 min at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Unbound antibodies were washed away with PBST and bound antibodies detected with 0.5 μg/mL HRP-conjugated goat anti-human constant kappa (2060-05, Southern Biotech, Birmingham, AL, USA) diluted in PBSTB ...
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bioRxiv - Microbiology 2021Quote: ... incubated with primary antibody (starting concentration 10 µg/ml) in PBS for 120 min and secondary antibody (anti-human IgG-HRP; Southern Biotech 2040-05) diluted 1:2500 in 1% BSA in PBS for 60 min at RT ...
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bioRxiv - Immunology 2020Quote: ... with excess of PBS-T before being incubated for one hour at RT with an alkaline phosphatase-conjugated goat anti-human IgG (Southern Biotech, Birmingham, AL), diluted 3000 × in PBS-T ...
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bioRxiv - Immunology 2020Quote: ... the plates were first washed 5 times with PBS-T and then incubated for 1 hour at RT with HRP-conjugated anti-human IgG (Fc-specific, clone JDC-10, Southern Biotech, Birmingham, AL) diluted 3000 × in PBS-T ...
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bioRxiv - Immunology 2020Quote: ... the plates were washed at least 5 times with PBS-T and then incubated for 1 hour at RT with HRP-conjugated anti-human IgG (Fc-specific, clone JDC-10, Southern Biotech, Birmingham, AL) diluted 3000 × in PBS-T ...
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bioRxiv - Immunology 2021Quote: ... plates were washed with PBS-T and HRP-conjugated goat anti-mouse IgG or goat anti-human IgG (Southern Biotech, Birmingham, AL, USA) added for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... plates were washed with PBS-T and HRP-conjugated goat anti-mouse IgG or goat anti-human IgG (Southern Biotech, Birmingham, AL, USA) added for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... and the cells were stained with 50 μL/well of a 1:1,000 dilution of goat anti-human IgG-PE (Southern Biotech, cat. # 2040-09) secondary antibodies for 30 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... and stained with a 1:1,000 solution of goat anti-human IgG antibodies conjugated to phycoerythrin (PE) (Southern Biotech, Cat# 2040-09; RRID:AB_2795648) at 4°C for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... the plates were incubated with HRP-conjugated goat anti-human IgG and IgA secondary Abs (Southern Biotech, IgG: 2044-05, IgA: 2053-05) and developed by the TMB substrate (ThermoFisher) ...
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bioRxiv - Immunology 2023Quote: ... and incubated with 0.75x serial dilutions of purified polyclonal human antibodies (IgG, SouthernBiotech, Cat # 0150-01; IgM, SouthernBiotech, Cat # 0158L-01; IgA, SouthernBiotech, Cat # 0155K-01) at known concentrations ...
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bioRxiv - Immunology 2023Quote: ... and the development of ADA in plasma samples were detected using a goat anti-human lambda IgG HRP-conjugated secondary antibody at 1:4000 dilution (Southern Biotech, Birmingham, AL). The plasma ADA levels were reported as endpoint titer ...
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bioRxiv - Genetics 2021Quote: ... Wells were washed as described and biotin conjugated goat anti-human IgG (Jackson AffiniPure #109-065-098; 1:30,000 or Southern Biotech 2049-08; 1:1,000) in PBS/1.0% BSA detection antibody was added to the wells at 100ul/well and incubated for 2 hours at room temperature with shaking ...
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bioRxiv - Biochemistry 2020Quote: ... the culture media were tested for their ACE2-Fc contents using a custom ELISA (anti-ACE2 antibody [Abcam, Cambridge, MA] for capturing and anti-human IgG-Fc-HRP [SouthernBiotech, Birmingham, AL] for detection). Clones with the highest expression of recombinant ACE2-Fc variants were selected ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed with water three times before applying 25 μl of secondary antibody (goat anti-human IgG Fc-Southern Biotech, 2048-04; goat anti-mouse IgG Fc-Southern Biotech, 1033-04) at a dilution of 1:4,000 in blocking buffer ...
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... slides were counterstained with DAPI for 5 min and enclosed in Fluoromount-G (0100-01; Southern Biotech).
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bioRxiv - Microbiology 2021Quote: ... Plates were then washed 5 times and incubated with goat anti-mouse Ig conjugated to HRP (SouthernBiotech) for 1 hour at room temp ...
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bioRxiv - Cell Biology 2022Quote: ... incubated in Hoechst for 5 min at RT and mounted with coverslips using Fluoromount-G (Southern Biotech). For wax preservation ...
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bioRxiv - Immunology 2022Quote: ... Nunc Immuno Plate MaxiSorp plates were coated with 5 μg/ml anti–Ig capture antibody (Southern biotech) and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: For the detection of antibody titers in sera and fecal supernatants 96-well plates were coated with goat anti-human Ig (H+L chain) antibody (Southern Biotech, Cat. No. 2010-01) or goat anti-human IgA Fab (Southern Biotech ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed twice with Fc stain buffer and stained with Alexa Fluor 647 goat anti-human IgG (Southern Biotech [Birmingham, AL], cat. 2016-31) in FACS buffer for 30 minutes at 4°C followed by 2 wash steps with FC stain buffer and resuspended in Fluorofix (BioLegend ...
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bioRxiv - Biochemistry 2021Quote: ... 500 μL of a secondary antibody solution (5 μM Alexa Fluor® 488 anti-mouse (SouthernBiotech; 1031-30) and 10 μM Alexa Fluor® 647 anti-human (SouthernBiotech ...
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bioRxiv - Cell Biology 2021Quote: ... FB lobes of approximately 5 larvae were mounted in 20 µl DAPI fluoromount-G (Southern Biotech 0100-20) per slide and imaged on an ApoTome.2 system using an Axiovert 200 equipped with a 63x 1.4 NA oil lens (Carl-Zeiss ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were washed four times for 5 min in PBS and embedded in DAPI Fluoromount-G (Southern Biotech).
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bioRxiv - Molecular Biology 2020Quote: ... the following secondary antibodies were used diluted 1:1000 in 5%-milk: anti-mouse (Southern Biotech, 1031–05) and a 1:1 mix of anti-rabbit (Southern Biotech ...
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bioRxiv - Biochemistry 2023Quote: ... or a 5-fold dilution series starting at 200ng/ml of purified IgG (Catalog #0107-01, Southern Biotech) or IgA (Catalog #0106-01 ...
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bioRxiv - Immunology 2020Quote: ... coated with 100 µL/well of goat anti-mouse IgA (5 µg/mL Bic 0.1M, pH 9.6; Southern Biotech) were incubated with serial 3-fold dilutions of either fecal supernatant (100 µL ...
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bioRxiv - Biophysics 2023Quote: ... at a 1:250 dilution and protein was detected with a HRP-conjugated goat anti-mouse antibody (Southern Biotech) using a 1:5000 dilution ...
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bioRxiv - Developmental Biology 2021Quote: ... The sections were then washed three times for 5 min with PBST and slides were mounted with Fluoromount-G containing DAPI and photobleaching inhibitors (SouthernBiotech). All steps were performed in a dark humid chamber to prevent evaporation and photobleaching ...
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bioRxiv - Immunology 2021Quote: ... at 1×106 cells/well and stimulated for 48 hours with 5 μg/mL of RBD-His or hCD81-His or media alone (RPMI + 5% FCS) in the presence of anti-mouse CD28 antibody [0,5 μg/ml] (Southern Biotech). As positive control cells were stimulated with anti-mouse CD3 [0,05 μg/ml] (Southern Biotech) ...