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Citations for SouthernBiotech :
551 - 600 of 719 citations for Goat Interleukin 6 IL6 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... plates were washed with PBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG1 antibodies (Southern Biotech) or HRP-conjugated goat anti-mouse IgG2a-HRP antibodies (Southern Biotech ...
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bioRxiv - Immunology 2024Quote: ... with serum diluted 1:10 followed by an incubation with goat anti-mouse IgG-FITC (1:100; Southern Biotech). Images were acquired on an inverted fluorescence microscope (Olympus) ...
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bioRxiv - Microbiology 2024Quote: ... Antibodies used for immunoblot analysis in this study were: anti-Human IgM goat polyclonal antibody (Southern Biotech #2020-01), anti-Human IgG goat polyclonal antibody (Southern Biotech #2040-01) ...
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bioRxiv - Microbiology 2024Quote: ... Immunoblot analysis was performed and human IgG was detected by Goat Anti-Human IgM-UNLB (Southern Biotech #2020-01) antibody.
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bioRxiv - Immunology 2023Quote: ... The IgG assay contained a goat anti-mouse IgG Fc antibody (SouthernBiotech, 1033-31, labeled with Alexa Fluor 488) and the IgM assay a donkey anti-mouse IgM F(ab’)2 (Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2021Quote: ... secondary antibody at RT for 2h and stained with 4’,6-diamidino-2-phenylindole (DAPI) before mounting onto slides with Fluoromount-G (Southern Biotech, Birmingham, AL).
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bioRxiv - Molecular Biology 2020Quote: ... Worms were rinsed in PBS (3×10mins, 3 × 1hr) and equilibriated in mounting media with 4’,6-diamidino-2-phenylindole DAPI (Fluoromount G, Southern Biotech, Birmingham, AL) overnight before mounting and imaging.
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bioRxiv - Immunology 2019Quote: ... PE goat anti-mouse IgG or PE mouse anti-human IgG (both from Southern Biotech, the latter for competition assay) was added to the plates and incubated for 1 hour at room temperature with mild agitation ...
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bioRxiv - Immunology 2020Quote: ... The assay was performed as described above except for the secondary antibodies (1:2,000. goat Anti-Mouse IgG2a cross absorbed HRP antibody, Southern biotech, 1083-05 ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 × 105 dendritic cells or B cells were incubated with different concentrations of C03 with or without goat anti-human Fc antibody (SouthernBiotech) for 48 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... Jurkat/NF-κB-GFP-hCD40 reporter cells were incubated with different concentrations of CD40 agonist antibodies with or without goat anti-human Fc antibody (SouthernBiotech) for 24 hours ...
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bioRxiv - Immunology 2021Quote: ... plates were washed with PBS-T and horseradish peroxidase (HRP)-conjugated goat anti-hamster IgG (Southern Biotech, Birmingham, AL, USA) or goat anti-ferret IgG (Abcam ...
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bioRxiv - Immunology 2021Quote: ... Wells were then washed 3 times with 200µL PBST before incubation for 1 hour with secondary antibodies (HRP-labeled Goat anti-mouse IgG1/IgG2b/IgG2c purchased from SouthernBiotech or HRP-labeled Goat anti-mouse IgG Fcγ purchased from Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2021Quote: ... Secondary staining was performed with a 1:10,000 dilution of HRP-conjugated pooled donkey antisera against goat IgG (H+L) (Southern Biotech) in PBS-T supplemented with 1% ELK for 60 minutes at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... Plates were then washed with TBST and incubated with secondary HRP-conjugated goat anti-human IgG (Southern Biotech, Birmingham, AL) at a 1:15,000 dilution for 45 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... and incubated with either rabbit anti-mouse IgG-HRP (Jackson Immuno) or goat anti-hamster IgG(H+L)-HRP (SouthernBiotech) diluted in casein for mouse or hamster samples ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 3x and incubated with goat anti-rhesus IgG-HRP antibody (Southern Biotech, 1:10,000 in blocking buffer) for 1 hr at RT ...
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Bacterial killing by complement requires direct anchoring of Membrane Attack Complex precursor C5b-7bioRxiv - Immunology 2019Quote: ... Secondary staining was performed with a 1:10.000 of HRP-conjugated pooled donkey antisera against goat IgG (H+L) (Southern Biotech) in PBS-T supplemented with 1% ELK for 60 minutes at 37 °C ...
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Bacterial killing by complement requires direct anchoring of Membrane Attack Complex precursor C5b-7bioRxiv - Immunology 2019Quote: ... Secondary staining was performed with a 1:5.000 of HRP-conjugated pooled donkey antisera against goat IgG (H+L) (Southern Biotech) in PBS-T supplemented with 1% BSA for 60 minutes at 37 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by detection with a 1:10,000 dilution of goat anti-human IgG-HRP (Southern Biotech 2040-05, Birmingham, AL). Plates were developed for 5 minutes with TMB substrate (VWR 95059-286) ...
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bioRxiv - Microbiology 2020Quote: ... membranes were washed with TBST and imaged (for Strep-Tag II HRP) or incubated with goat anti-rabbit-HRP (1:5,000; Southern Biotech).
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bioRxiv - Immunology 2020Quote: ... Antibody was detected using an HRP-conjugated Goat anti-Mouse IgG kappa secondary antibody (Southern Biotech, PA1-86015, 1:1000) and developed with 1-Step Turbo TMB ELISA Substrate (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were stimulated with buffer (1% FBS/HBSS) or 10 μg/mL goat F(ab’)2 anti-mouse Igκ (SouthernBiotech) and Indo-1 fluorescence (violet vs blue ...
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bioRxiv - Immunology 2020Quote: ... bound IgG was detected by incubating with 1:1000 polyclonal goat anti human IgG-HRP (Southern Biotech, Birmingham, AL, USA) for 1h at 37°C ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed three times and incubated with horseradish peroxidase (HRP)-conjugated goat anti-human secondary antibody (ITK Southern Biotech) diluted 1:2000 in blocking buffer for 1 hour at RT ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 5 times with PBS-T and incubated with goat anti-mouse IgG1 antibody conjugated with horseradish peroxidase (SouthernBiotech) for 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Bound IgM monoclonal antibody was detected with PE-labelled goat anti-mouse IgM secondary antibody (Southern Biotech, Birmingham, AL, US) (Halpin et al. ...
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bioRxiv - Immunology 2022Quote: ... plates were washed with TBS containing 0.05% Tween-20 (TBST) and resuspended in 50 μL of Goat-anti-human IgG-PE (Southern Biotech). After 2 hours of incubation on a rotator at room temperature ...
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bioRxiv - Immunology 2022Quote: ... IgG antibodies were detected using horseradish peroxidase (HRP)-conjugated goat anti-human IgG detection antibody (Southern Biotech, Birmingham, AL, USA) at a 1:4,000 dilution and colorimetric development was accomplished using 100 μL of 0.1% 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid ...
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bioRxiv - Microbiology 2022Quote: ... the plate was incubated in goat anti-mouse horseradish peroxidase (GAM-HRP) at a 1:5000 ratio (SouthernBiotech, Birmingham, AL). Lastly ...
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bioRxiv - Immunology 2023Quote: ... another microarray was developed with the second detection mix: goat anti-human kappa chain Alexa555 (Southern Biotech, Ref.:2060-32) and goat anti-human lambda chain Alexa647 (Southern Biotech ...
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bioRxiv - Immunology 2023Quote: Biotinylated Goat F(ab)2 anti-human isotype antibodies (anti-human IgM, SouthernBiotech, Cat # 2022-01; anti-human IgA, SouthernBiotech, Cat # 2052-01 ...
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bioRxiv - Molecular Biology 2023Quote: ... the arrays were incubated with a mixture of goat anti-mouse IgM HRP (10 µg/mL; #1021-05 Southern Biotech) and donkey anti-goat antibody labeled with AlexaFluor555 (5 µg/mL ...
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bioRxiv - Microbiology 2023Quote: ... Bound antibodies were detected by using goat anti-mouse IgG conjugated to horseradish peroxidase (HRP; Southern Biotech, Birmingham, AL, USA) diluted 1:2000 in PBST and developed using 3,3’,5,5’-tetramethylbenzidine substrate (Thermo Scientific Pierce ...
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bioRxiv - Immunology 2023Quote: ... 25 μl of undiluted supernatant was added to wells coated with 1 μg/ml goat anti-mouse Ig (Southern Biotech) or HAPR8 and for serum samples 3-fold serial dilutions of serum samples starting at 1/100 were added to wells coated with NP4-BSA (10 μg/ml ...
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bioRxiv - Bioengineering 2023Quote: ... The staining procedure involved the following steps: (1) The sections were blocked in a solution of 10% normal goat serum (SouthernBiotech) and 0.3% Triton-X in PBS for 1 hour at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Membranes were incubated in TBST containing 5% (w/v) skimmed milk with goat anti-mouse IgG conjugated to horseradish peroxidase (HRP) (1:2000, 1010-05, SouthernBiotech) for 3h at 4°C in a rotary platform and washed thrice with TBST for 10 min each time ...
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bioRxiv - Immunology 2024Quote: ... wells were washed six times with PBS supplemented with 0.1 % Tween20 and incubated for 2 h with 1 μg/mL biotinylated goat α-mouse IgM or IgG1 (SouthernBiotech) at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... washed 6 times and then incubated with 1: 6000 dilution of horseradish peroxidase (HRP) conjugated anti-mouse IgG secondary antibody (Southern Biotech; Birmingham, AL, USA) for 1 h at RT ...
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bioRxiv - Microbiology 2020Quote: ... IgG-bound DNP-beads were next incubated with 1 µg/mL Alexa Fluor647-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech) in PBS-TH.
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bioRxiv - Microbiology 2020Quote: ... Antibody binding was detected using directly labeled IgG or Alexa Fluor647-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech). Samples were measured using flow cytometry (BD FACSVerse ...
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bioRxiv - Immunology 2021Quote: ... were coated with 2.5 µg/ml of mouse anti-human c-Myc 9E10 mAb (produced in house) or goat anti-human IgG (1:2000, Southern Biotech) for the standard ...
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bioRxiv - Genetics 2021Quote: ... Primary antibodies (1:250 Anti-Fibronectin-1, F3648, Sigma-Aldrich; 1:100 Goat Anti-Type I Collagen, 1310-01, Southern Biotech; 1:100 Goat Anti-Type V Collagen ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 4X in wash buffer and the secondary antibody Goat anti-Human Ig-HRP (Southern Biotech, Cat# 2010-05), was added and incubated at 37°C for 1 hr ...
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bioRxiv - Immunology 2021Quote: ... This will be followed by a 1 h incubation with biotinylated HRP conjugated goat anti-mouse IgG subtype antibodies (Southern Biotech). 3,3’,5,5’ tetramethylbenzidine (TMB ...
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bioRxiv - Immunology 2019Quote: ... Sera were incubated 2 h at 37°C on wells pre-coated with 5 μg/mL of NP15-BSA or goat-anti-mouse IgM (Southern Biotech) and saturated with PBS 2% BSA ...
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bioRxiv - Immunology 2019Quote: ... The arrays were washed 3x 300 μL/well on the plate washer before adding 100 μL/well of a 1/5000 cocktail of biotinylated secondary polyclonal goat antibodies (Southern Biotech) directed to the human kappa and lambda light chains in assay diluent ...
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bioRxiv - Immunology 2019Quote: ... culture supernatants were incubated 2 h at 37°C on wells pre-coated with 5 μg/mL of goat-anti-mouse IgM (Southern Biotech) and saturated with PBS 2% BSA ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... before adding 50 μL/well of a 1:5000 dilution of HRP-conjugated goat anti-mouse IgG (Southern Biotech, #1030-05) in TBSF+MnCl2 ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed with water three times before applying 25 μl of secondary antibody (goat anti-human IgG Fc-Southern Biotech, 2048-04 ...