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Citations for SouthernBiotech :
351 - 400 of 586 citations for Glutamate Decarboxylase 2 GAD2 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... They were washed in blocking buffer 2X for 2 hours and imaged in situ or mounted on microscope slides using Fluoromount-G (Southern Biotech). Immunostaining Analysis ...
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bioRxiv - Immunology 2022Quote: ... a standard curve for mouse isotype was established for each plate by coating 2 columns with 50 μL goat anti-mouse Ig (1 μg/mL in PBS; Southern Biotech) overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... bacteria were incubated with a Goat F(ab’)2 anti-human Kappa-Alexa Fluor 647 (pre-diluted in PBS 0.1%BSA; 2.5 μg/mL, Southern Biotech #2062-31) to detect bound Fab fragments ...
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bioRxiv - Immunology 2019Quote: ... the membrane was re-blocked with 5% BSA in 1x TBST for 2 hours at room temperature before probing with 1:5000 dilution of anti-human IgG-HRP (Southern Biotech) in 5% BSA/1x TBST for 1 hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... or IgG purified from Ggta1+/+ or Ggta1-/- mouse serum (60 µg/mL, PBS 2% BSA, wt/vol, 30 min.) followed by anti-mouse IgG-PE (Southern Biotech #1030-09 ...
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bioRxiv - Immunology 2020Quote: ... 500 ng of F(ab’)2 fragments of α-μ (clone JDC-15; Dako [α-human]; OB1022; Southern Biotech [α-mouse]) and α-CD19 (clone HD37 ...
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bioRxiv - Immunology 2020Quote: ... for detection of αGal and IgG purified from Ggta1+/+ or Ggta1-/- mouse serum (60 µg/mL, PBS 2% BSA, wt/vol, 30 min.) followed by anti-mouse IgG-PE (Southern Biotech #1030-09 ...
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bioRxiv - Neuroscience 2020Quote: ... Nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) with the use of DAPI Fluoromount-G (0100-20; Southern Biotech). Sections were also stained for β-galactosidase (β-gal ...
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bioRxiv - Immunology 2021Quote: ... B cells were then cultured for a further 2 days in RPMI (as described above) or supplemented with 500ng/ml Igκ (Southern Biotech). Where indicated the following reagents were added into in vitro cultures ...
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bioRxiv - Bioengineering 2021Quote: ... arrays were incubated for an additional 2 h at RT and 60 rpm with goat anti-mouse IgG conjugated to HRP (Southern Biotech) diluted 1:5000 in blocking solution ...
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bioRxiv - Microbiology 2020Quote: ... Guts were washed 5 times in PBS-T and then mounted on slides in Flouromount plus 4’,6-diamidino-2-phenylindole nuclear stain (DAPI, Southern Biotech) and sealed with a cover slip ...
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bioRxiv - Biochemistry 2020Quote: ... The immunoreactivity was detected by incubation with Rabbit F(ab’)2 Anti-Goat IgG(H+L)-HRP (6020-05, Southern Biotech) diluted 3000 × in PBS-T.
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bioRxiv - Immunology 2024Quote: ... membranes were incubated with primary antibodies overnight at 4 °C and during 2 hours at room temperature with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit IgG (SouthernBiotech, USA). Membranes were then revealed using G:BOX Chemi XL1.4 Fluorescent & Chemiluminescent Imaging System (Syngene ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed again and the nuclei were stained with DAPI (4’,6-diamidino-2-phenylindole) before mounting with Fluoromount-G (Southern Biotech). Primary antibodies against histone H3 methylation on lysine 4 ...
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bioRxiv - Cancer Biology 2024Quote: ... permeabilized with 0.1% Triton X-100 (Fisher Biotech, Hampton, NH, USA), and blocked with PBS supplemented with goat serum (2%, v/v) (Southern biotech) and 0.1% Tween-20 (Thermo Fisher) ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips with stained cells were washed 2 x 5 min in PBS and then mounted with Fluoromount-G (Southern Biotech 0100-01) for image acquisition ...
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bioRxiv - Microbiology 2022Quote: ... the beads were incubated with 1 µg/mL Alexa Fluor647-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech, 2062-31) in PBS-TH for 30 min at 4 °C with shaking ...
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bioRxiv - Immunology 2022Quote: ... were coated overnight at 4°C with 2 μg/mL of monkey cross-adsorbed goat anti-human IgG (Southern Biotech #2049-01) diluted in phosphate-buffered saline (PBS) ...
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bioRxiv - Biochemistry 2022Quote: ... and then treated with alkaline phosphatase (AP)-conjugated goat anti-rabbit-IgG (Southern Biotech; 1:8000 in TBST with 2% (w/v) bovine serum albumin) ...
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bioRxiv - Physiology 2020Quote: ... The sections were processed for immunostaining of Pan-CK (--) as described above with 4’,6-diamidino-2-phenylindole (DAPI) containing Fluormount-G (SouthernBiotech, Birmingham, AL) to visualize nuclei.
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bioRxiv - Microbiology 2020Quote: ... and then incubated with 1:100000 secondary antibody Anti-Mouse Kappa conjugated to Horseradish Peroxidase (Southern Biotech) for 2 h before washing again ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies for immunoblotting were: horseradish peroxidase (HRP)-conjugated goat anti-rat IgG (Southern Biotech 3010-05), HRP-conjugated goat anti-mouse IgG (Southern Biotech 1010-05) ...
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bioRxiv - Cell Biology 2020Quote: ... secondary antibodies were incubated for 1 hour at room temperature before mounting in Fluoromount-G medium (SouthernBiotech) for immunochemistry ...
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bioRxiv - Neuroscience 2019Quote: ... 10 min) with PBST (0.3%) following each antibody incubation and finally mounted with DAPI Fluoromount-G (SouthernBiotech). Fluorescent images of the retinal sections were taken with a CCD camera connected to a Zeiss inverted fluorescence microscope controlled by AxioVision software.
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bioRxiv - Immunology 2019Quote: ... Rabbit antibodies were detected using alkaline phosphatase conjugated goat anti-rabbit IgG (Southern Biotech, Cat. #4030-04) or IgA (Novus ...
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bioRxiv - Bioengineering 2021Quote: ... and a secondary antibody of goat anti-rabbit IgG-HRP (1:3,000 dilution) (Southern Biotech, Birmingham, AL) for detection of VIN CP-FcAL ...
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bioRxiv - Neuroscience 2021Quote: ... They were then incubated with secondary antibodies (Table 1) and mounted in Fluoromount (SouthernBiotech, Birmingham, AL, USA) for observation.
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bioRxiv - Microbiology 2020Quote: ... Antibody concentration was determined by comparing absorbance values to known concentrations of mouse IgG (0107-01, SouthernBiotech) bound to the plate with goat anti-mouse Ig (1010-01 ...
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bioRxiv - Microbiology 2022Quote: ... Bound antibodies were detected with horseradish peroxidase (HRP)-conjugated goat F(ab’)₂ anti-human kappa (Southern Biotech) in 1% BSA in PBS-T and Tetramethylbenzidine as substrate ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated goat-anti-mouse isotype-specific (IgM, IgG) antibodies (50 μL at 0.1 μg/mL; Southern Biotech) were added and incubated at RT for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... after which the intensity was enhanced using a tertiary rabbit anti-goat HRP-conjugated antibody (Southern Biotech). AEC (3-Amino-9-EthylCarbazole ...
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bioRxiv - Immunology 2020Quote: ... bound antibody was detected using HRP-conjugated goat anti-human Fc multiple species cross-absorbed (Southern Biotech) for 1 h at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Bound serum antibodies were detected with polyclonal biotinylated goat anti-mouse IgM or anti-IgG1 (Southern Biotech), streptavidin-alkaline phosphatase conjugate (Roche ...
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bioRxiv - Bioengineering 2022Quote: ... Secreted IgG was detected by binding to HRP-conjugated goat anti-human IgG secondary antibody (Southern Biotech). Spots were developed with AEC Substrate Kit ...
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bioRxiv - Immunology 2020Quote: ... The cells were stained for surface expression of IgA using PE-conjugated anti-mouse IgA antibody (SouthernBiotech) and sorted by a FACS Aria IIIu and influx sorter (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... at the indicated timepoints serum was collected and analyzed by ELISA for anti-OVA antibodies (Southern Biotech).
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bioRxiv - Cancer Biology 2021Quote: ... HRP tagged secondary antibodies (Anti-rabbit IgG, Santa Cruz, Dallas, TX, Anti-human IgG, SouthernBiotech, Birmingham, AL) were used for the protein detection and membrane images were taken using Amersham Imager 600 (GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2021Quote: ... The bound antibodies were detected using goat anti-human IgG conjugated with horseradish peroxidase (HRP) (Southern Biotech, cat ...
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bioRxiv - Immunology 2020Quote: ... The bound antibodies were detected using goat anti-human IgG conjugated with HRP (horseradish peroxidase) (Southern Biotech) and TMB (3,3µ,5,5µ-tetramethylbenzidine ...
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bioRxiv - Immunology 2020Quote: ... HRP-conjugated goat anti-mouse IgG second antibody (catalog number 1030-05, Southern Biotech, Birmingham, AL, USA) was added ...
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bioRxiv - Molecular Biology 2021Quote: ... IgM was immunoprecipitated from lysates or media samples with a goat anti-mouse IgM antibody (Southern Biotech). Immunoprecipitates were washed with lysis buffer and eluted from beads with reducing SDS-PAGE sample buffer and analyzed by SDS-PAGE ...
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bioRxiv - Cell Biology 2022Quote: ... Antibody binding was visualized with peroxidase-conjugated sheep anti-rabbit immunoglobulin (Southern Biotech; 4010-05; 1:15000), or sheep anti-mouse immunoglobulin (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... Nunc Immuno Plate MaxiSorp plates were coated with 5 μg/ml anti–Ig capture antibody (Southern biotech) and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Slides were exposed to biotin labelled goat Fab anti-human IgG secondary antibody (Southern Biotech, Birmingham, AL) at a 1:2500 dilution for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... and 50 µL of HRP-conjugated anti- hamster IgG(H+L) antibody (Southern Biotech Cat. #6061-05) diluted 1:1000 in blocking solution ...
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bioRxiv - Immunology 2023Quote: ... plates were incubated with horseradish peroxidase (HRP)-conjugated anti-human IgG secondary antibody (Southern Biotech, 2042-05) diluted 1:4000 in PBS containing 0.05% Tween 20 and 1% BSA for 45 min at RT ...
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bioRxiv - Cell Biology 2022Quote: ... Antibody binding was visualized with peroxidase-conjugated sheep anti-rabbit immunoglobulin (Southern Biotech; 4010-05; 1:15000), sheep anti-mouse immunoglobulin (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... Secondary antibody (Goat Anti-Mouse IgG-HRP or IgA-HRP [SouthernBiotech #1030-05 and #1040-05, respectively]) was added at a 1:1000 dilution in PBS with 0.1% Tween and 5% FBS overnight at 4°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... the cells were incubated with an anti-human IgG antibody conjugated to Alexa Fluor-488 (Southern Biotech Catalog# 2015-30 ...
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bioRxiv - Immunology 2023Quote: ... then incubated with an HRP-conjugated human/bovine/horse cross-adsorbed anti-mouse secondary antibody (Southern Biotech) at a 1:20,000 dilution for 1 hour at RT ...