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Citations for SouthernBiotech :
551 - 600 of 689 citations for Flap endonuclease 1 FEN 1 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... at a 1:250 dilution and protein was detected with a HRP-conjugated goat anti-mouse antibody (Southern Biotech) using a 1:5000 dilution ...
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bioRxiv - Immunology 2019Quote: ... PE goat anti-mouse IgG or PE mouse anti-human IgG (both from Southern Biotech, the latter for competition assay) was added to the plates and incubated for 1 hour at room temperature with mild agitation ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 × 105 dendritic cells or B cells were incubated with different concentrations of C03 with or without goat anti-human Fc antibody (SouthernBiotech) for 48 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... Jurkat/NF-κB-GFP-hCD40 reporter cells were incubated with different concentrations of CD40 agonist antibodies with or without goat anti-human Fc antibody (SouthernBiotech) for 24 hours ...
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bioRxiv - Immunology 2022Quote: ... Plates were then washed with TBST and incubated with secondary HRP-conjugated goat anti-human IgG (Southern Biotech, Birmingham, AL) at a 1:15,000 dilution for 45 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... MagPlex beads were washed with PBST (PBS, 0.05% Tween-20) and incubated with anti-human IgG Fc-PE (Southern Biotech). Finally ...
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bioRxiv - Immunology 2021Quote: ... the pellet was resuspended in a 50 μL mixture of PE-anti-human CD11b (Southern Biotech, 10 μL/million cells), Alexa Fluor 647-anti-human CD35 (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... washed thrice with PBST and detected with HRP-conjugated mouse anti-human IgG (Fc only) (Southern Biotech, Birmingham, AL, USA) antibody at a 1:2000 dilution ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed three times and incubated with horseradish peroxidase (HRP)-conjugated goat anti-human secondary antibody (ITK Southern Biotech) diluted 1:2000 in blocking buffer for 1 hour at RT ...
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bioRxiv - Immunology 2022Quote: ... plates were washed with TBS containing 0.05% Tween-20 (TBST) and resuspended in 50 μL of Goat-anti-human IgG-PE (Southern Biotech). After 2 hours of incubation on a rotator at room temperature ...
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bioRxiv - Immunology 2022Quote: ... IgG antibodies were detected using horseradish peroxidase (HRP)-conjugated goat anti-human IgG detection antibody (Southern Biotech, Birmingham, AL, USA) at a 1:4,000 dilution and colorimetric development was accomplished using 100 μL of 0.1% 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid ...
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bioRxiv - Immunology 2023Quote: ... another microarray was developed with the second detection mix: goat anti-human kappa chain Alexa555 (Southern Biotech, Ref.:2060-32) and goat anti-human lambda chain Alexa647 (Southern Biotech ...
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bioRxiv - Microbiology 2023Quote: ... was used except that plates were coated with anti-human kappa and lambda light chain specific mouse antibodies (Southern Biotech) at 1:1 ratio diluted to 1 μg/ml in PBS overnight ...
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bioRxiv - Microbiology 2024Quote: ... Antibodies used for flow cytometry analysis performed in this study were: FITC-conjugated anti-human IgM (Southern Biotech #2020-02), goat anti-human IgG-UNLB (Southern Biotech #2040-01) ...
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bioRxiv - Immunology 2021Quote: ... plates were washed and probed at room temperature for 1 hour with 1μg/ml of biotinylated anti-IgG (Southern Biotech) in PBS 2.5%FCS ...
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bioRxiv - Immunology 2020Quote: ... The assay was performed as described above except for the secondary antibodies (1:2,000. goat Anti-Mouse IgG2a cross absorbed HRP antibody, Southern biotech, 1083-05 ...
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bioRxiv - Neuroscience 2019Quote: ... Reactions were quenched with water and then washed twice in 1× TBE prior to coverslipping with fluoromount G (Southern Biotech).
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bioRxiv - Immunology 2021Quote: ... Wells were then washed 3 times with 200µL PBST before incubation for 1 hour with secondary antibodies (HRP-labeled Goat anti-mouse IgG1/IgG2b/IgG2c purchased from SouthernBiotech or HRP-labeled Goat anti-mouse IgG Fcγ purchased from Jackson ImmunoResearch ...
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bioRxiv - Genetics 2020Quote: ... the strips were incubated with horse radish peroxidase (HRP)-conjugated anti-rhesus secondary antibodies (Southern biotech, 6200-50, 1:2000) for 2 additional hours at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Secondary staining was performed with a 1:10,000 dilution of HRP-conjugated pooled donkey antisera against goat IgG (H+L) (Southern Biotech) in PBS-T supplemented with 1% ELK for 60 minutes at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 3x and incubated with goat anti-rhesus IgG-HRP antibody (Southern Biotech, 1:10,000 in blocking buffer) for 1 hr at RT ...
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bioRxiv - Microbiology 2020Quote: ... followed by addition of 50 μL of 1:1000 anti-hamster-IgG(H+L)-HRP (Southern Biotech Cat. #6061-05) in PBST or PBSTF or 1:1000 anti-hamster-IgG2/IgG3-HRP in PBST or PBSTF (Southern Biotech Cat ...
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Bacterial killing by complement requires direct anchoring of Membrane Attack Complex precursor C5b-7bioRxiv - Immunology 2019Quote: ... Secondary staining was performed with a 1:10.000 of HRP-conjugated pooled donkey antisera against goat IgG (H+L) (Southern Biotech) in PBS-T supplemented with 1% ELK for 60 minutes at 37 °C ...
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Bacterial killing by complement requires direct anchoring of Membrane Attack Complex precursor C5b-7bioRxiv - Immunology 2019Quote: ... Secondary staining was performed with a 1:5.000 of HRP-conjugated pooled donkey antisera against goat IgG (H+L) (Southern Biotech) in PBS-T supplemented with 1% BSA for 60 minutes at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... fixed blood cells were washed in bicarbonate buffer and stained using antibodies against CD71 (Southern Biotech, #1720-02, 1:200) and Ter119 (eBioscience ...
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The antibiotic bedaquiline activates host macrophage innate immune resistance to bacterial infectionbioRxiv - Immunology 2019Quote: ... coverslips were set in Fluoromount G medium containing 1 µg/ml 4′,6-diamidino-2-phenylindole (DAPI) (SouthernBiotech, Birmingham, Alabama) on microscope slides ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed 3x and incubated with a 1:50,000 dilution of anti-mouse total IgG-HRP antibody (Southern Biotech) and incubated for 1h at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... membranes were washed with TBST and imaged (for Strep-Tag II HRP) or incubated with goat anti-rabbit-HRP (1:5,000; Southern Biotech).
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bioRxiv - Immunology 2020Quote: ... The membrane was washed in PBS containing Tween-20 (0.05%) and was incubated for 1 h with horseradish peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (Southern Biotech) diluted 1:20,000 accordingly ...
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bioRxiv - Immunology 2020Quote: ... Antibody was detected using an HRP-conjugated Goat anti-Mouse IgG kappa secondary antibody (Southern Biotech, PA1-86015, 1:1000) and developed with 1-Step Turbo TMB ELISA Substrate (ThermoFisher ...
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bioRxiv - Biochemistry 2020Quote: ... each well was incubated with 50 μL diluted 1:5,000 in BSA5-PBS-T of anti-rabbit-HRP (SouthernBiotech, USA) or anti-mouse-HRP (Rockland Immunochemicals ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were stimulated with buffer (1% FBS/HBSS) or 10 μg/mL goat F(ab’)2 anti-mouse Igκ (SouthernBiotech) and Indo-1 fluorescence (violet vs blue ...
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bioRxiv - Immunology 2022Quote: ... A volume of 25 μL of a 1:1,000 dilution of Mouse Anti-Biotin-AP (Southern Biotech, cat. #6404-04) in blocking buffer was added to each of the wells of the HN plates and incubated for 1 hr at RT ...
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bioRxiv - Microbiology 2022Quote: ... the plate was incubated in goat anti-mouse horseradish peroxidase (GAM-HRP) at a 1:5000 ratio (SouthernBiotech, Birmingham, AL). Lastly ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed 3x with DPBS and coverslips mounted on 1 mm glass slides using Fluoromount-G (SouthernBiotech, #0100-01). Cells were imaged using a Zeiss Axio Observer 7 fitted with a 40x objective using DAPI and Alexa Fluor 488 filters ...
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bioRxiv - Cell Biology 2023Quote: ... All coverslips were then washed 3 times in PBS before being mounted on a drop of mounting medium (DAPI fluoromount – G 1:1000; SouthernBiotech)) on labeled glass coverslips ...
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bioRxiv - Immunology 2023Quote: ... 25 μl of undiluted supernatant was added to wells coated with 1 μg/ml goat anti-mouse Ig (Southern Biotech) or HAPR8 and for serum samples 3-fold serial dilutions of serum samples starting at 1/100 were added to wells coated with NP4-BSA (10 μg/ml ...
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bioRxiv - Bioengineering 2023Quote: ... The staining procedure involved the following steps: (1) The sections were blocked in a solution of 10% normal goat serum (SouthernBiotech) and 0.3% Triton-X in PBS for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... 0,1 % BSA and washed three times with 1× PBS before mounting on microscope slides using Fluoromount-G (Cat# 0100-01, SouthernBiotech,). Hoechst 33342 dye (Cat# B2261 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 × 105 cells in 100 μL per well were plated in a 96-well plate pre-coated with anti-mouse CD3ε (1 μg/mL, SouthernBiotech). Tumors were harvested from mice 10 days post inoculation and dissociated using gentleMACS (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2024Quote: ... Coverslips were washed in 1× PBS and mounted on glass slides with Fluoromount G (Southern Biotech, with or without DAPI).
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bioRxiv - Immunology 2024Quote: ... Membranes were incubated in TBST containing 5% (w/v) skimmed milk with goat anti-mouse IgG conjugated to horseradish peroxidase (HRP) (1:2000, 1010-05, SouthernBiotech) for 3h at 4°C in a rotary platform and washed thrice with TBST for 10 min each time ...
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bioRxiv - Immunology 2024Quote: ... wells were washed six times with PBS supplemented with 0.1 % Tween20 and incubated for 2 h with 1 μg/mL biotinylated goat α-mouse IgM or IgG1 (SouthernBiotech) at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... Antibody binding was detected using directly labeled IgG or Alexa Fluor647-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech). Samples were measured using flow cytometry (BD FACSVerse ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 4X in wash buffer and the secondary antibody Goat anti-Human Ig-HRP (Southern Biotech, Cat# 2010-05), was added and incubated at 37°C for 1 hr ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed with water three times before applying 25 μl of secondary antibody (goat anti-human IgG Fc-Southern Biotech, 2048-04 ...
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bioRxiv - Immunology 2022Quote: ... IgG bound to the rSARS-CoV-2 S protein was detected using a horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody (Southern Biotech) incubated for 1 hour ± 10 minutes at 24°C ± 2°C ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed three times and incubated with the goat anti-human-IgG Fc secondary antibody conjugated with alkaline phosphatase (AP, Southern Biotech) at a 1:1000 dilution in blocking buffer for 1 h at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... bacteria were incubated with a Goat F(ab’)2 anti-human Kappa-Alexa Fluor 647 (pre-diluted in PBS 0.1%BSA; 2.5 μg/mL, Southern Biotech #2062-31) to detect bound Fab fragments ...
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bioRxiv - Systems Biology 2022Quote: ... for capture and biotinylated anti-human insulin antibody (Eli Lilly and Company) with horseradish peroxidase conjugated streptavidin (7100-05, Southern Biotech) for detection ...