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Citations for SouthernBiotech :
101 - 150 of 908 citations for Fab ZAP mouse Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Antibody concentration was determined by comparing absorbance values to known concentrations of mouse IgG (0107-01, SouthernBiotech) bound to the plate with goat anti-mouse Ig (1010-01 ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated goat-anti-mouse isotype-specific (IgM, IgG) antibodies (50 μL at 0.1 μg/mL; Southern Biotech) were added and incubated at RT for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... Bound serum antibodies were detected with polyclonal biotinylated goat anti-mouse IgM or anti-IgG1 (Southern Biotech), streptavidin-alkaline phosphatase conjugate (Roche ...
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bioRxiv - Immunology 2020Quote: ... The cells were stained for surface expression of IgA using PE-conjugated anti-mouse IgA antibody (SouthernBiotech) and sorted by a FACS Aria IIIu and influx sorter (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... HRP-conjugated goat anti-mouse IgG second antibody (catalog number 1030-05, Southern Biotech, Birmingham, AL, USA) was added ...
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bioRxiv - Molecular Biology 2021Quote: ... IgM was immunoprecipitated from lysates or media samples with a goat anti-mouse IgM antibody (Southern Biotech). Immunoprecipitates were washed with lysis buffer and eluted from beads with reducing SDS-PAGE sample buffer and analyzed by SDS-PAGE ...
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bioRxiv - Immunology 2023Quote: ... Secondary antibody (Goat Anti-Mouse IgG-HRP or IgA-HRP [SouthernBiotech #1030-05 and #1040-05, respectively]) was added at a 1:1000 dilution in PBS with 0.1% Tween and 5% FBS overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... then incubated with an HRP-conjugated human/bovine/horse cross-adsorbed anti-mouse secondary antibody (Southern Biotech) at a 1:20,000 dilution for 1 hour at RT ...
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bioRxiv - Microbiology 2024Quote: ... The following secondary antibodies were used: rabbit-anti mouse IgG-horseradish peroxidase (HRP; SouthernBiotech; dilution 1:10‘000) and goat-anti rabbit IgG-HRP (SouthernBiotech ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies used include the following: Goat Anti-Mouse IgG (H+L; Southern Biotech, 1031-05, 1:2,000) and Goat Anti-Rabbit IgG (Life Technologies ...
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bioRxiv - Biophysics 2020Quote: The anti-mouse-Igκ antibody for clustering BCRs was purchased from Southern Biotech (Birmingham, AL; catalogue #1050-01). AF647-conjugated anti-mouse-IgM Fab fragments (catalogue #115-607-020 ...
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bioRxiv - Biochemistry 2021Quote: ... 500 μL of a secondary antibody solution (5 μM Alexa Fluor® 488 anti-mouse (SouthernBiotech; 1031-30) and 10 μM Alexa Fluor® 647 anti-human (SouthernBiotech ...
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bioRxiv - Microbiology 2021Quote: ... incubated with a horseradish peroxidase-conjugated goat anti-mouse IgG secondary antibody (SouthernBiotech, 1030-05, 1:2000 dilution) at RT for 1 h ...
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bioRxiv - Immunology 2020Quote: ... Detection was performed using an HRP-linked goat anti-rabbit or goat anti-mouse secondary antibody (Southern Biotech) and chemiluminescence detection (ECL Plus™ ...
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bioRxiv - Molecular Biology 2020Quote: ... the following secondary antibodies were used diluted 1:1000 in 5%-milk: anti-mouse (Southern Biotech, 1031–05) and a 1:1 mix of anti-rabbit (Southern Biotech ...
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bioRxiv - Immunology 2021Quote: ... the bound antibodies were detected with alkaline phosphatase-conjugated isotype-specific goat anti-mouse polyclonal serum (Southern Biotech).
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bioRxiv - Immunology 2022Quote: ... beads were incubated for 1 hr with PE-conjugated mouse anti-human IgG antibodies (JDC-10, Southern Biotech). After three washes ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed 3x with PBS and treated with an AP conjugated anti-mouse IgG antibody (Southern Biotech) for 1.5 hours ...
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bioRxiv - Immunology 2023Quote: ... and incubated for 1 hour with mouse anti-monkey IgG HRP-conjugated secondary antibody (Southern Biotech, clone SB108a) at a 1:5,000 dilution or anti-monkey IgM HRP-conjugated secondary antibody (Rockland ...
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bioRxiv - Biochemistry 2023Quote: ... incubated with an appropriate peroxidase-labeled secondary antibody (anti-mouse IgG1-HRP #1070-05, Southern Biotech, Birmingham, USA) for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... and incubated for 1 h with mouse anti-rhesus IgG HRP-conjugated secondary antibody (Southern Biotech, clone SB108a) at a 1:5,000 dilution or anti-rhesus IgM HRP-conjugated secondary antibody (Rockland ...
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bioRxiv - Immunology 2024Quote: ... IgG3 and IgA levels were detected by using HRP-coupled goat anti-mouse subtype-specific secondary antibodies (SouthernBiotech).
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bioRxiv - Immunology 2021Quote: ... Plates were then washed three times with PBS-T and secondary goat anti-mouse IgG-HRP antibody (Southern Biotech), diluted 1:4000 in ELISA buffer ...
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bioRxiv - Microbiology 2021Quote: ... samples were diluted at 1:50 and antigen-coupled microspheres were incubated with secondary antibody diluted at 1:100 each: mouse anti-human IgG1 hinge (0.1 mg/ mL, clone 4E3, SouthernBiotech); IgG2 Fc (0.1 mg/ mL ...
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bioRxiv - Microbiology 2020Quote: ... Plates were again washed with PBST and coated with 50 μL 1:10,000 diluted secondary antibody goat anti-mouse IgG HRP conjugated (1030-05, SouthernBiotech) and incubated 1 hr at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Plates were then washed three times with PBS-T and secondary goat anti-mouse IgG-HRP antibody (Southern Biotech), diluted 1:4000 in ELISA buffer ...
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bioRxiv - Immunology 2021Quote: ... For the three-step protocol sections were stained using a goat anti-mouse IgG1-HRP-conjugated antibody (Southern Biotech) for CXCL12 and goat anti-mouse IgG2b-HRP-conjugated antibody (Southern Biotech ...
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bioRxiv - Immunology 2020Quote: ... HRP-conjugated secondary antibodies were used as follows: goat anti-mouse IgG-HRP (SouthernBiotech, Cat: 1030-05; 1:10,000) or goat anti-mouse IgA-HRP (SouthernBiotech ...
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bioRxiv - Immunology 2020Quote: ... the plates were incubated with a 1:2,000 dilution of a goat anti-mouse IgG2a-HRP antibody (Southern Biotech) in PBS supplemented with 1% FCS and incubated with gently shaking at RT for one hour ...
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bioRxiv - Biophysics 2023Quote: ... at a 1:250 dilution and protein was detected with a HRP-conjugated goat anti-mouse antibody (Southern Biotech) using a 1:5000 dilution ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were stained again for 30 min with 0.5 µg of FITC-conjugated anti-mouse IgA antibody (Southern Biotech) in 100 µl of staining buffer to refresh and harmonize the staining between batches ...
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bioRxiv - Bioengineering 2024Quote: ... plates were washed with PBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG1 antibodies (Southern Biotech) or HRP-conjugated goat anti-mouse IgG2a-HRP antibodies (Southern Biotech ...
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bioRxiv - Immunology 2023Quote: ... The IgG assay contained a goat anti-mouse IgG Fc antibody (SouthernBiotech, 1033-31, labeled with Alexa Fluor 488) and the IgM assay a donkey anti-mouse IgM F(ab’)2 (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2021Quote: ... Wells were then washed 3 times with 200µL PBST before incubation for 1 hour with secondary antibodies (HRP-labeled Goat anti-mouse IgG1/IgG2b/IgG2c purchased from SouthernBiotech or HRP-labeled Goat anti-mouse IgG Fcγ purchased from Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed 3x and incubated with a 1:50,000 dilution of anti-mouse total IgG-HRP antibody (Southern Biotech) and incubated for 1h at 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... and then for 1 hour at room temperature with a 1:4000 diluted rabbit anti-mouse HRP secondary antibody (SouthernBiotech Cat# 6170-05 ...
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bioRxiv - Immunology 2021Quote: ... at 1×106 cells/well and stimulated for 48 hours with 5 μg/mL of RBD-His or hCD81-His or media alone (RPMI + 5% FCS) in the presence of anti-mouse CD28 antibody [0,5 μg/ml] (Southern Biotech). As positive control cells were stimulated with anti-mouse CD3 [0,05 μg/ml] (Southern Biotech) ...
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bioRxiv - Immunology 2020Quote: ... The membrane was washed in PBS containing Tween-20 (0.05%) and was incubated for 1 h with horseradish peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (Southern Biotech) diluted 1:20,000 accordingly ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 5 times with PBS-T and incubated with goat anti-mouse IgG1 antibody conjugated with horseradish peroxidase (SouthernBiotech) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... was used except that plates were coated with anti-human kappa and lambda light chain specific mouse antibodies (Southern Biotech) at 1:1 ratio diluted to 1 μg/ml in PBS overnight ...
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bioRxiv - Microbiology 2023Quote: ... Bound antibodies were detected by using goat anti-mouse IgG conjugated to horseradish peroxidase (HRP; Southern Biotech, Birmingham, AL, USA) diluted 1:2000 in PBST and developed using 3,3’,5,5’-tetramethylbenzidine substrate (Thermo Scientific Pierce ...
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bioRxiv - Biochemistry 2024Quote: ... PVDF membranes containing blotted proteins were washed in excess PBS-Tween 20 for 1h at room temperature before application of a 1:2000 dilution of primary mouse Anti-Human IgA1 antibodies conjugated to biotin (SouthernBiotech). Blots were washed 10 min 3 x in PBS followed by treatment with a 1:2000 dilution of ExtrAvidin®−Peroxidase conjugate (Sigma ...
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bioRxiv - Immunology 2022Quote: Serum from uninfected mice was analyzed for total IgG1 using a mouse clonotyping kit and standards (Southern Biotech) according to manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... washing with wash buffer (1xPBS+0.05% Tween) and incubation with secondary antibody - Mouse-anti-monkey IgG-HRP (Southern Biotech, 4700-05) for 1h ...
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bioRxiv - Immunology 2021Quote: ... This will be followed by a 1 h incubation with biotinylated HRP conjugated goat anti-mouse IgG subtype antibodies (Southern Biotech). 3,3’,5,5’ tetramethylbenzidine (TMB ...
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bioRxiv - Microbiology 2020Quote: ... followed by the addition of horseradish peroxidase-conjugated goat anti-mouse-IgG Fc secondary antibody (1:2000) (Southern Biotech, 1033-05). After incubation for 2 hours at room temperature ...
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bioRxiv - Immunology 2020Quote: ... plates were coated with 2 µg/mL of goat anti-human kappa or goat anti-human lambda antibodies cross-absorbed against mouse IgG (Southern Biotech) at 4°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... Secondary antibodies used include goat anti-mouse IgG and goat anti rabbit-IgG both conjugated to horseradish peroxidase (HRP) (Southern Biotech). Forte Western HRP substrate (Millipore ...
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bioRxiv - Immunology 2021Quote: ... Bound IgA or IgM antibody was detected by incubation with anti-IgA-alkaline phosphatase (goat anti-mouse IgA (α chain-specific) (SouthernBiotech) or anti-IgM-alkaline phosphatase goat anti-mouse IgM (μ chain-specific ...
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bioRxiv - Immunology 2020Quote: An ELISA to detect anti-CCHFV Ig was performed as previously described (Hawman et al., 2019) with an anti-mouse Ig detection antibody (Southern Biotech) to detect all immunoglobulin isotypes or anti-mouse IgG or IgM (Southern Biotech ...