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Citations for SouthernBiotech :
1 - 50 of 268 citations for Estrone 3 Glucuronide E1G ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... ELISA plates were coated with 2 µg/ml of goat anti-human IgG (Southern Biotech) at 4°C overnight ...
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bioRxiv - Genetics 2022Quote: ... Plates were washed using ELISA wash buffer and incubated with HRP-conjugated anti-Ig secondary antibodies (Southern Biotech) for 2 hours in humid storage at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Nunc Immuno Plate MaxiSorp plates were coated with 5 μg/ml anti–Ig capture antibody (Southern biotech) and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... ELISA plates were washed as described above and 100μl of secondary goat anti-rat IgG (SouthernBiotech Cat. 3030-04), goat anti-rat IgM (SouhternBiotech Cat ...
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bioRxiv - Immunology 2022Quote: ... plates were washed 4× with ELISA wash buffer and a 1:4000 dilution of goat anti-mouse IgG (SouthernBiotech, catalog #1030-05) in ELISA blocking buffer was added to each well and incubated at 37°C for 1 hour followed by 4 washes with ELISA wash buffer ...
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bioRxiv - Neuroscience 2021Quote: ... further washed 3×5 min in PBS and mounted in Fluoromount-G (SouthernBiotech, 0100-01). Images were acquired with a Zeiss LSM700 confocal laser scanning microscope and assembly was performed with ImageJ/Fiji ...
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bioRxiv - Neuroscience 2023Quote: ... The slides were washed 3 × 5 mins and mounted with Fluoromount-G (SouthernBiotech, Cat # 0100-01) on microscope slides with Secure-Seal spacer (Invitrogen ...
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bioRxiv - Immunology 2021Quote: To detect mouse Ig Nunc MaxiSorp ELISA plates were coated for 1h at room temperature with goat anti-mouse Ig (H+L) (4 μg/ml; Southern Biotech, Cat#1010-01; RRID: AB_2794121). Citrobacter rodentium-specific Ig was quantified by coating Nunc MaxiSorp ELISA plates with 108 bacteria/well heat-inactivated at 60°C for 1 h ...
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bioRxiv - Immunology 2021Quote: ... the same ELISA was performed but using the secondary antibodies from SBA Clonotyping System-HRP kit (SouthernBiotech, 5300-05, RRID:AB_2796080) diluted accordingly to manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Plates were then washed 5 times and incubated with goat anti-mouse Ig conjugated to HRP (SouthernBiotech) for 1 hour at room temp ...
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bioRxiv - Developmental Biology 2023Quote: ... slides were washed 2 x for 5 minutes in PBS-tx and 3 x for 5 minutes in PBS and cover slipped with DAPI Fluoromount-G (SouthernBiotech).
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bioRxiv - Immunology 2020Quote: ... plates were washed 4 times with PBS-T and an anti-monkey IgG conjugated to horseradish peroxidase (Southern Biotech 4700-5) 1 hour at 37°C ...
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bioRxiv - Immunology 2022Quote: ... NP-OVA specific IgG1 was detected as previously described48 using plates were coated with TNP(5)-BSA and detected using goat anti-mouse IgG1-HRP (Southern Biotech).
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bioRxiv - Evolutionary Biology 2019Quote: ... Slides were then washed in PBS + 0.1% Triton 3 × 10 min and PBS 1 × 5 min then coverslipped with Fluoromount G (Southern biotech Cat OB10001) under glass cover slips ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed with PBS (3 x 5 minutes) and mounted on standard microscope glass slides using DAPI-Fluoromount G (SouthernBiotech, 0100-20). Antibodies and dilutions used were as follows ...
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bioRxiv - Neuroscience 2022Quote: ... A final wash of 3 × 5 minutes was performed prior to mounting using DAPI Fluoromount-G® (Southern Biotech, Cat No. 0100-20). Slices were imaged on a Zeiss LSM 800 laser confocal scan head mounted on a Zeiss Z1Axio Observer inverted microsope frame (Carl Zeiss ...
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bioRxiv - Microbiology 2023Quote: ... Coverslips were then incubated in secondary antibody in blocking buffer for 1 h at RT followed by 3×5 min washes in PBS and then mounted in Fluoromount G with DAPI (Southern Biotech, Birmingham, AL).
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bioRxiv - Immunology 2020Quote: ... ELISAs were performed using alternate secondary antibodies (Sigma-Aldrich; Jackson ImmunoResearch; Southern Biotech). End point titers were extrapolated from sigmoidal 4PL (where X is log concentration ...
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bioRxiv - Immunology 2023Quote: ... and ELISAs were detected with HRP-conjugated goat-anti-mouse IgG1-HRP (Southern Biotech). To develop the enzymatic reaction TMB was used as described above ...
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bioRxiv - Immunology 2019Quote: ... 96-well Immulon 4 HBX plates were coated overnight at 4°C with 5 mg/ml polyclonal goat-anti mouse κ (Southern Biotech; 1050-01). Nonspecific binding was blocked with 1% bovine serum albumin in PBS and samples were incubated at 37°C ...
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bioRxiv - Immunology 2023Quote: ... and IgA concentrations were measured by ELISA using the SBA Clono-typing System (Southern Biotech).
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bioRxiv - Immunology 2020Quote: ... at the indicated timepoints serum was collected and analyzed by ELISA for anti-OVA antibodies (Southern Biotech).
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bioRxiv - Immunology 2024Quote: ... we used the following secondary antibodies during ELISA: goat anti-mouse IgG1 HRP antibody (#1071-05, SouthernBiotech), goat anti-mouse IgG2a HRP antibody (#1081-05 ...
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bioRxiv - Microbiology 2019Quote: ... IgG1,2A,2B,3 or IgM (Southern Biotech) were added to 96 well plates at a dilution of 1:4,000 in 3% BSA and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... ∼ 3 ul of DAPI-Fluoromount-G (SouthernBiotech) was added to each well and a coverslip placed over the slide ...
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bioRxiv - Immunology 2023Quote: ... ELISA antibodies for measuring total and HDM-specific Igs in serum were purchased from Southern Biotech (Birmingham, AL). ELISA data were analyzed using SoftMax Pro software (Molecular Devices).
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bioRxiv - Immunology 2019Quote: ... the same ELISA procedure as described above was performed except using anti-human IgA and anti-human IgA-HRP antibodies (SouthernBiotech, AL). Corresponding immunoglobulin isotypes were used as standards after serial dilutions.
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bioRxiv - Immunology 2023Quote: ... The concentration of Ig in serum supernatants was measured using a standard sandwich ELISA with anti-human Ig primary (Cat#: 2048-01, Southern Biotech) and secondary antibodies (Cat# ...
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bioRxiv - Molecular Biology 2020Quote: ... Worms were rinsed in PBS (3×10mins, 3 × 1hr) and equilibriated in mounting media with 4’,6-diamidino-2-phenylindole DAPI (Fluoromount G, Southern Biotech, Birmingham, AL) overnight before mounting and imaging.
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bioRxiv - Immunology 2020Quote: ... all serum samples were diluted to 1 total IgG ELISA unit and then detected with anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam). The results of the IgG subclass ELISA are presented using OD values instead of the ELISA units used for the total IgG ELISA ...
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bioRxiv - Immunology 2022Quote: ... Plates were coated with either goat anti-mouse IgG (SouthernBiotech), NP30-BSA and NP7-BSA (Biosearch Technologies) ...
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bioRxiv - Biochemistry 2020Quote: ... the culture media were tested for their ACE2-Fc contents using a custom ELISA (anti-ACE2 antibody [Abcam, Cambridge, MA] for capturing and anti-human IgG-Fc-HRP [SouthernBiotech, Birmingham, AL] for detection). Clones with the highest expression of recombinant ACE2-Fc variants were selected ...
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bioRxiv - Immunology 2022Quote: ... plates were pre-coated with goat anti-mouse IgM (Southern Biotech) or with NP(15)-BSA (Biosearch Technologies) ...
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bioRxiv - Immunology 2021Quote: ... plates were developed using 3,3′,5,5′-Tetramethylbenzidine substrate (SouthernBiotech, 0410-01) and the reaction was stopped using TMB stop solution (SouthernBiotech ...
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bioRxiv - Immunology 2020Quote: ... plates were washed and incubated with ABTS peroxide substrate solution (SouthernBiotech) for 15 minutes or until color change occurred ...
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bioRxiv - Immunology 2020Quote: ... plates were washed and incubated with streptavidin-alkaline phosphatase (Southern Biotech) for 2 hours at room temperature ...
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bioRxiv - Immunology 2022Quote: ... plates were washed and 100μl of anti-mouse IgA-HRP (SouthernBiotech) added followed by incubation at 37°C for 2 hours ...
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bioRxiv - Immunology 2023Quote: ... plates were coated with anti-mouse Ig (ref: 0107-01, SouthernBiotech) on 96-well high-binding EIA/RIA plates overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... rinsed 3×10’ with PBST and mounted using Fluoromount-G (Southern Biotech). All steps performed at room temperature unless otherwise noted ...
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bioRxiv - Immunology 2019Quote: ... were detected with bromo-4-chloro-3-indolyl phosphate substrate (Southern Biotech).
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bioRxiv - Bioengineering 2022Quote: ... 5 µL of blocking antibody (anti-IgG, Southern Biotech) was added to the 50 µL cell solution to yield a final concentration of 25 µg/mL ...
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bioRxiv - Bioengineering 2023Quote: ... (2) block sections in 5% normal goat serum (SouthernBiotech) and 0.3% Triton-X solution in PBS for 1h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... bound to the plate with goat anti-mouse Ig (1010-01, SouthernBiotech). Serum assays were performed in duplicate for each mouse.
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bioRxiv - Microbiology 2020Quote: ... plates were incubated with biotinylated anti-IgG or anti-IgA (Southern Biotech) followed by incubation with streptavidin conjugated horseradish peroxidase (Jackson ImmunoResearch) ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed and a goat anti-mouse IgG (Southern Biotech) coupled to alkaline phosphatase (Jackson Immunoresearch ...
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bioRxiv - Immunology 2024Quote: ... The plates were developed using α-mouse IgA-AP Abs (Southern Biotech) followed by 4-Nitropenyl phosphate disodium salt hexahydrate substrate tablets (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... 50 µm mouse brain sections were rinsed in PBS for 5 minutes and blocked with PBS/5% normal goat serum (Southern Biotech, 0060-01) for 2 hours at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... 3) anti dog IgG-HRP as linker (Southern BioTech goat anti-canine IgG) and 4 ...
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bioRxiv - Cell Biology 2021Quote: ... washed 3 times in PBS and mounted in Fluoromount-G® (Southern Biotech). Images were acquired using a TCS SP8 (Leica ...
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bioRxiv - Immunology 2021Quote: ... washed 3 times in PBS and mounted in Fluoromount-G® (Southern Biotech). Images were acquired using a TCS SP8 (Leica ...