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Citations for SouthernBiotech :
1 - 50 of 89 citations for Cow Protein L Isoaspartate PCMT1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: To detect mouse Ig Nunc MaxiSorp ELISA plates were coated for 1h at room temperature with goat anti-mouse Ig (H+L) (4 μg/ml; Southern Biotech, Cat#1010-01; RRID: AB_2794121). Citrobacter rodentium-specific Ig was quantified by coating Nunc MaxiSorp ELISA plates with 108 bacteria/well heat-inactivated at 60°C for 1 h ...
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bioRxiv - Immunology 2021Quote: ... the same ELISA was performed but using the secondary antibodies from SBA Clonotyping System-HRP kit (SouthernBiotech, 5300-05, RRID:AB_2796080) diluted accordingly to manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... ELISAs were performed using alternate secondary antibodies (Sigma-Aldrich; Jackson ImmunoResearch; Southern Biotech). End point titers were extrapolated from sigmoidal 4PL (where X is log concentration ...
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bioRxiv - Immunology 2023Quote: ... and ELISAs were detected with HRP-conjugated goat-anti-mouse IgG1-HRP (Southern Biotech). To develop the enzymatic reaction TMB was used as described above ...
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bioRxiv - Immunology 2023Quote: ... and IgA concentrations were measured by ELISA using the SBA Clono-typing System (Southern Biotech).
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bioRxiv - Microbiology 2024Quote: ... ELISA plates were coated with 2 µg/ml of goat anti-human IgG (Southern Biotech) at 4°C overnight ...
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bioRxiv - Immunology 2019Quote: ... Anti-mouse Ig(H+L) (Southern Biotech, Birmingham, AL) and serial dilutions of mouse IgM and IgG2c (Southern Biotech ...
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bioRxiv - Immunology 2020Quote: ... at the indicated timepoints serum was collected and analyzed by ELISA for anti-OVA antibodies (Southern Biotech).
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bioRxiv - Immunology 2024Quote: ... we used the following secondary antibodies during ELISA: goat anti-mouse IgG1 HRP antibody (#1071-05, SouthernBiotech), goat anti-mouse IgG2a HRP antibody (#1081-05 ...
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bioRxiv - Developmental Biology 2023Quote: ... Donkey anti-Rat IgG(H+L) AF555 (Southern Biotech #643032) and Donkey anti-Rabbit IgG(H+L ...
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bioRxiv - Genetics 2022Quote: ... Plates were washed using ELISA wash buffer and incubated with HRP-conjugated anti-Ig secondary antibodies (Southern Biotech) for 2 hours in humid storage at 4°C ...
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bioRxiv - Immunology 2023Quote: ... ELISA antibodies for measuring total and HDM-specific Igs in serum were purchased from Southern Biotech (Birmingham, AL). ELISA data were analyzed using SoftMax Pro software (Molecular Devices).
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bioRxiv - Microbiology 2021Quote: ... ELISA plates were washed as described above and 100μl of secondary goat anti-rat IgG (SouthernBiotech Cat. 3030-04), goat anti-rat IgM (SouhternBiotech Cat ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit Anti-Sheep IgG(H+L)-AP (Southern biotech #6150.04, 1:2000) was used along with NBT (#S380C ...
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bioRxiv - Immunology 2019Quote: ... the same ELISA procedure as described above was performed except using anti-human IgA and anti-human IgA-HRP antibodies (SouthernBiotech, AL). Corresponding immunoglobulin isotypes were used as standards after serial dilutions.
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bioRxiv - Immunology 2023Quote: ... The concentration of Ig in serum supernatants was measured using a standard sandwich ELISA with anti-human Ig primary (Cat#: 2048-01, Southern Biotech) and secondary antibodies (Cat# ...
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bioRxiv - Immunology 2021Quote: ... plates were coated with goat anti-mouse IgM + IgG + IgA (H+L) (SouthernBiotech) diluted in 1X PBS ...
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bioRxiv - Cell Biology 2023Quote: ... and goat α-rabbit IgG(H+L)-HRP (no. 4030-05, Southern Biotech). Cycloheximide was from Enzo Life Sciences.
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bioRxiv - Cell Biology 2024Quote: ... Goat Anti-Mouse IgG(H+L) Human ads-HRP (Southern Biotech, 1:5,000), Goat Anti-Rabbit IgG(H+L ...
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bioRxiv - Cell Biology 2024Quote: ... Goat Anti-Rabbit IgG(H+L) Human ads-HRP (Southern Biotech, 1:5000), Strep-Tag II Antibody HRP Conjugate (Sigma Aldrich-71591-3 ...
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bioRxiv - Immunology 2022Quote: ... plates were washed 4× with ELISA wash buffer and a 1:4000 dilution of goat anti-mouse IgG (SouthernBiotech, catalog #1030-05) in ELISA blocking buffer was added to each well and incubated at 37°C for 1 hour followed by 4 washes with ELISA wash buffer ...
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bioRxiv - Immunology 2020Quote: ... all serum samples were diluted to 1 total IgG ELISA unit and then detected with anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam). The results of the IgG subclass ELISA are presented using OD values instead of the ELISA units used for the total IgG ELISA ...
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bioRxiv - Biochemistry 2020Quote: ... the culture media were tested for their ACE2-Fc contents using a custom ELISA (anti-ACE2 antibody [Abcam, Cambridge, MA] for capturing and anti-human IgG-Fc-HRP [SouthernBiotech, Birmingham, AL] for detection). Clones with the highest expression of recombinant ACE2-Fc variants were selected ...
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FOXO dictate initiation of B cell development and myeloid restriction in common lymphoid progenitorsbioRxiv - Immunology 2022Quote: ... were coated with 10-20µg/ml of anti-mouse immunoglobulin (Ig) (H+L) (Southern Biotech) to capture total Ig in the serum ...
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bioRxiv - Immunology 2023Quote: ... One hundred μl horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (H+L) (Southern Biotech) at 1:4000 dilution in 0.1% BSA/TBST was added and incubated for 1 h ...
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bioRxiv - Immunology 2024Quote: ... were pre-coated with 2μg/mL of goat-α-mouse Ig(H+L) (Southern Biotech) diluted in PBS ...
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bioRxiv - Immunology 2023Quote: ... + Human IgM-L (cat# 0158L-01, southern biotech) + Human IgA-k (cat# 0155K-01, Southern Biotech). Goat anti-human IgM-AP (cat# 2020-04 ...
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bioRxiv - Cell Biology 2023Quote: ... The coverslips were mounted on poly-L-lysine-coated slides using DAPI-Fluoromount G (Southern Biotech). Imaging was as described for fluorescence microscopy above ...
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bioRxiv - Cell Biology 2021Quote: ... Goat Anti-Rabbit IgG(H+L) Mouse/Human ads-HRP (4050-05, dilution 1:10000) (Southern Biotech); Alexa 488- ...
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bioRxiv - Immunology 2022Quote: ... Cells were further incubated with biotinylated goat anti-rabbit IgG (H+L) and HRP-streptavidin (Southern Biotech). The reactions were developed with o-Phenylenediamine di-hydrochloridec (OPD ...
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bioRxiv - Biochemistry 2022Quote: ... Secondary incubations included horseradish-peroxidase-conju-gated rabbit anti-goat IgG (H+L) (Southern Biotech 6164-05), Streptavidin-HRP (Cell Signaling Tech #3999) ...
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bioRxiv - Microbiology 2023Quote: ... and 50 µL of HRP-conjugated anti- hamster IgG(H+L) antibody (Southern Biotech Cat. #6061-05) diluted 1:1000 in blocking solution ...
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bioRxiv - Cancer Biology 2024Quote: ... Na2CO3 1.59g/L pH 9.6) and 1μg/mL of unlabeled total anti-human total Ig (Southern Biotech) overnight at 4C or at 37C for one hour ...
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bioRxiv - Developmental Biology 2022Quote: ... legs expressing fluorescent proteins were mounted in Fluoromount 50 (SouthernBiotech). For immunohistochemistry ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies used include the following: Goat Anti-Mouse IgG (H+L; Southern Biotech, 1031-05, 1:2,000) and Goat Anti-Rabbit IgG (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... were coated with either αGal-BSA (Dextra) or goat anti-mouse Ig(H+L) (Southern Biotech #1010-01) (5 µg/mL in Carbonate-Bicarbonate buffer ...
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bioRxiv - Microbiology 2021Quote: ... for C5b6 with a 1:5.000 of HRP-conjugated donkey antisera against goat IgG (H+L) (Southern Biotech) and for sMAC with 1:5,000 HRP-conjugated streptavidin (Southern Biotech) ...
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bioRxiv - Immunology 2023Quote: ... Membranes were then incubated with HRP-conjugated goat anti-rabbit IgG (H+L) (1:4000 dilution; Southern Biotech) at RT for 1 h ...
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bioRxiv - Immunology 2021Quote: ... High affinity binding plates were coated with purified Ab (0.5 μg/well) directed against Ig H+L (Southern Biotech) for IgG classes or IgE (BD Pharmingen ...
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bioRxiv - Cell Biology 2021Quote: ... dilution 1:10,000) and Goat Anti-Mouse IgG (H + L) Human ads-HRP (1031-05, dilution 1:10,000) (Southern Biotech); sheep anti-TGN38 (AHP499G ...
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bioRxiv - Immunology 2021Quote: ... and permeabilized prior to staining with a FITC-conjugated goat anti-rhesus IgG (H+L) polyclonal antibody (Southern Biotech). Data represent the frequency of cells positive for IgG-binding to Env for post-vaccination samples compared to the pre-vaccination sample ...
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bioRxiv - Microbiology 2022Quote: ... or 1:5,000 mouse anti-His-Tag (primary) then 1:5000 goat anti-mouse IgG(H+L)-HRP (secondary) (SouthernBiotech). Blots were developed with ECL Western Blotting Substrate (Pierce ...
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bioRxiv - Immunology 2021Quote: ... followed by the addition of horseradish peroxidase (HRP)-conjugated goat anti-Hamster IgG (H+L) (1:2000 dilution) (SouthernBiotech) for an hour at RT ...
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bioRxiv - Microbiology 2022Quote: ... a GAM IgG (H + L) conjugated to horseradish peroxidase was used as a secondary Ab (1:1000; Southern Biotech) and incubated with TBST (5% BSA ...
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bioRxiv - Developmental Biology 2022Quote: ... The tissues were washed with PBT and rinsed with PBS and then the wing imaginal discs were mounted on poly-L-lysine-coated coverslips using Fluoromount-G (SouthernBiotech). Tissues were imaged using either a Leica LSM 510 or a Nikon C1 upright confocal microscope and processed using ImageJ (version 2.0 ...
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bioRxiv - Microbiology 2021Quote: ... Secondary staining was performed with a 1:10,000 dilution of HRP-conjugated pooled donkey antisera against goat IgG (H+L) (Southern Biotech) in PBS-T supplemented with 1% ELK for 60 minutes at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... and incubated with either rabbit anti-mouse IgG-HRP (Jackson Immuno) or goat anti-hamster IgG(H+L)-HRP (SouthernBiotech) diluted in casein for mouse or hamster samples ...
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bioRxiv - Microbiology 2020Quote: ... followed by addition of 50 μL of 1:1000 anti-hamster-IgG(H+L)-HRP (Southern Biotech Cat. #6061-05) in PBST or PBSTF or 1:1000 anti-hamster-IgG2/IgG3-HRP in PBST or PBSTF (Southern Biotech Cat ...
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Bacterial killing by complement requires direct anchoring of Membrane Attack Complex precursor C5b-7bioRxiv - Immunology 2019Quote: ... Secondary staining was performed with a 1:10.000 of HRP-conjugated pooled donkey antisera against goat IgG (H+L) (Southern Biotech) in PBS-T supplemented with 1% ELK for 60 minutes at 37 °C ...
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Bacterial killing by complement requires direct anchoring of Membrane Attack Complex precursor C5b-7bioRxiv - Immunology 2019Quote: ... Secondary staining was performed with a 1:5.000 of HRP-conjugated pooled donkey antisera against goat IgG (H+L) (Southern Biotech) in PBS-T supplemented with 1% BSA for 60 minutes at 37 °C ...