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Citations for SouthernBiotech :
701 - 739 of 739 citations for Beta Tubulin Rabbit Recombinant mAb HRP conjugated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Following additional washings and incubation at 37°C for 1 h with AP-conjugated anti-goat IgG secondary antibody (Southern Biotech), absorbance was read by addition of PNPP ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed and incubated with a peroxidase-conjugated goat anti-mouse anti-IgM or anti-IgG antibody (Southern Biotech). After washing ...
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bioRxiv - Immunology 2023Quote: ... Bound IL-21 was subsequently detected with mouse anti-human IL-21-biotin (clone 2B2-G20, eBioscience) followed by alkaline phosphatase-conjugated streptavidin (Southern Biotech). The signals were compared to a standard curve generated with recombinant human IL-21 (R&D Systems).
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bioRxiv - Immunology 2024Quote: ... In other experiments, we used as secondary antibodies isotype-specific (IgA, IgG1, IgG2a, IgG2b, IgG3) alkaline-phosphatase-conjugated antibodies (Southern Biotech). Bound antibodies were detected using p-nitrophenyl phosphate (Sigma ...
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bioRxiv - Genetics 2020Quote: ... followed by incubation with Alexa-488 anti-rabbit secondary antibody (1:1000, #6441-30 SouthernBiotech) for 2 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... the tissues were washed with PBS and incubated with rabbit-anti-mouse antibody (Southern Biotech) for 30 minutes at room temperature followed by poly-HRP-anti-rabbit IgG (DPVR110HRP ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibody binding was detected with rabbit anti-mouse IgG1-APC (Southern Biotech, Birmingham, USA). To assess binding of recombinant CXCL12γ ...
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bioRxiv - Immunology 2019Quote: ... Murine antibodies were detected using alkaline phosphatase conjugated goat anti-mouse IgG or IgA (Southern Biotech, Cat #1030-04 and 1040-04). Rabbit antibodies were detected using alkaline phosphatase conjugated goat anti-rabbit IgG (Southern Biotech ...
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bioRxiv - Microbiology 2022Quote: ... the beads were incubated with 1 µg/mL Alexa Fluor647-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech, 2062-31) in PBS-TH for 30 min at 4 °C with shaking ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed twice in FACS buffer and stained with 50 μl/well of 1:200 dilution of R-phycoerythrin (PE)-conjugated mouse anti-human IgG Fc antibody (SouthernBiotech #9040-09) and 1:1000 dilution of Zombie-NIR viability dye (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... the cells were stained with 50μl/well of 1:200 diluted R-phycoerythrin (PE)-conjugated mouse anti-human IgG Fc antibody (SouthernBiotech cat.# 9040-09) and 1:1000 dilution of Zombie-NIR viability dye (BioLegend cat.# 423105 ...
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bioRxiv - Neuroscience 2022Quote: ... The next day slides were washed and incubated for 1 hr in a secondary biotin-conjugated anti-mouse antibody (1:200, goat anti mouse, SouthernBiotech, Birmingham, AL) mixed in blocking solution ...
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bioRxiv - Immunology 2023Quote: ... IgG3 was measured following 6 washes and a 1-h incubation with 100 μL/well 1:5,000 mouse anti-human biotin-conjugated IgG3 hinge (SouthernBiotech, Birmingham, Alabama, USA) then 6 washes and 1-h incubation with 100 μL/well 1:40,000 HRP-conjugated streptavidin (Jackson ImmunoResearch Labs) ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were then incubated with AlexaFluor™-conjugated secondary antibodies for two hours at room temperature and briefly rinsed with PBSTr and mounted with DAPI (Southern Biotech).
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bioRxiv - Microbiology 2021Quote: ... samples were further statically incubated for 1 h at 4°C with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech, 1:300) and 6 μM Syto9 (Live/Dead BacLight Bacterial Viability Kit ...
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bioRxiv - Bioengineering 2021Quote: ... Antibodies bound to the thymocytes were detected by Cy5-conjugated goat anti-mouse IgG (1:250 dilution, 1030-15, Southern Biotech, Birmingham, AL) and Alexa Fluor 488-conjugated goat anti-mouse IgM (1:250 dilution ...
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bioRxiv - Immunology 2020Quote: ... with excess of PBS-T before being incubated for one hour at RT with an alkaline phosphatase-conjugated goat anti-human IgG (Southern Biotech, Birmingham, AL), diluted 3000 × in PBS-T ...
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bioRxiv - Immunology 2021Quote: ... For detection of IgG subclasses all serum samples were diluted to 1 total IgG EU and incubated at 37 °C for 1 h prior to detection with Alkaline Phosphatase conjugated anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam) incubated for 1 h at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... followed by two rounds of fluorescence-activated cell sorting (FACS) of cells stained with FITC-Conjugated rat anti-mouse IgG1 (Southern Biotech 1144-02), and rounds of soft-agar cloning in SeaPlaque agarose ...
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bioRxiv - Immunology 2022Quote: ... and stained with a 1:1,000 solution of goat anti-human IgG antibodies conjugated to phycoerythrin (PE) (Southern Biotech, Cat# 2040-09; RRID:AB_2795648) at 4°C for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... cells were washed twice with FACS buffer and incubated for 30 min at 4°C with 1:1000 diluted PE-conjugated goat F(ab)’2 anti-human IgG (Southern Biotech 2042-09) in the dark ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed and incubated with either AP-conjugated goat anti-mouse IgG or IgM antibody (SouthernBiotech; cat # 1030-04, 1020-04) (Supplementary Table 8) ...
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bioRxiv - Neuroscience 2020Quote: ... or Alexa fluor™ 488 anti-rabbit mounted on slides and embedded in fluoromount (SouthernBiotech, AL).
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bioRxiv - Neuroscience 2023Quote: ... anti-sheep (Novus NBP1-73267) or a 1:1 mix of anti-rabbit (Southern Biotech; OB405005) and anti-rabbit (Cell Signaling ...
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bioRxiv - Genetics 2021Quote: ... Wells were washed as described and biotin conjugated goat anti-human IgG (Jackson AffiniPure #109-065-098; 1:30,000 or Southern Biotech 2049-08; 1:1,000) in PBS/1.0% BSA detection antibody was added to the wells at 100ul/well and incubated for 2 hours at room temperature with shaking ...
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bioRxiv - Immunology 2020Quote: ... Horse spleen ferritin-specific IgA levels in the supernatant of the faecal homogenates was determined by enzyme-linked immunosorbent assay in plates coated with horse spleen ferritin followed by detection with horseradish peroxidase-conjugated goat anti-mouse IgA (Southern Biotech, Birmingham, AL, USA) using optEIA (BD Biosciences ...
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bioRxiv - Physiology 2023Quote: ... The membranes were then washed 3x in TBST before incubation for 1 hour at room temperature with peroxidase-conjugated secondary antibodies in TBST at 1:10,000 (Southern Biotech, #1031-05, #4050-05). Antibody binding was detected using an enhanced chemiluminescence HRP substrate detection kit (Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... Secondary incubations included horseradish-peroxidase-conju-gated rabbit anti-goat IgG (H+L) (Southern Biotech 6164-05), Streptavidin-HRP (Cell Signaling Tech #3999) ...
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bioRxiv - Immunology 2022Quote: ... all sourced from BEI Resources) and an Alexa Fluor 647 labeled goat anti-rabbit IgG (Southern Biotech) secondary in addition to Aqua (BD Biosciences ...
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bioRxiv - Plant Biology 2024Quote: ... Washed membranes were incubated in a 1:6000 dilution of goat anti-rabbit secondary antibody (Southern Biotech) conjugated to alkaline phosphatase in 3% (w/v ...
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bioRxiv - Bioengineering 2021Quote: ... for CD4 and goat anti-rabbit (1:50 dilution for 30 minutes, 4010-05, Southern Biotech, Birmingham, AL) for CD8 ...
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bioRxiv - Genetics 2020Quote: ... washed with PBT and then incubated with Alexa-488 anti-Rabbit secondary antibody (#6441-30 SouthernBiotech, 1:250) for 2h ...
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bioRxiv - Genomics 2021Quote: ... probed again by a goat anti-rabbit secondary antibody (Southern Biotech, catalog # 4030-05, and 1:200 dilution), and developed using a DAB kit (Abcam ...
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bioRxiv - Plant Biology 2023Quote: ... and the secondary antibody used against other primary antibodies was a goat anti-rabbit IgG (4052-05, SouthernBiotech).
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed with 1% BSA in PBS twice before incubating with secondary anti-rat IgG antibody conjugated with APC (1:500; Southern Biotech, AL, USA, Catalog no. 3010-11L). Cells were analyzed in BD FACSVerse™ flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... membranes were incubated with secondary antibodies conjugated to alkaline phosphatase anti-IgM or anti-IgG diluted 1:2000 (rabbit anti-mouse IgM, Jackson ImmunoResearch, goat anti-mouse IgG, SouthernBiotech). Substrate NBT/BCIP (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies including anti-mouse immunoglobulin G (IgG; 1010-05) and anti-rabbit IgG (catalog no. 4010-05) were obtained from Southern biotech. Primary antibodies were used at a 1:1000 dilution (unless otherwise stated) ...
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bioRxiv - Cell Biology 2020Quote: ... Then samples were washed three times with 0.1% Triton X-100 in PBS for 5 minutes and were incubated 30 minutes at RT with the following secondary antibody diluted 1:200: goat anti-rabbit Alexa Fluor 488 (Southern Biotech 4050-30), goat anti-mouse Alexa Fluor 488 (Southern Biotech 1030-30 ...
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bioRxiv - Bioengineering 2021Quote: ... The slides were subsequently incubated at room temperature with secondary antibodies: goat anti-rabbit Ig (1:100 dilution for 15 minutes, 4010-05, Southern Biotech, Birmingham, AL) for CD4 and goat anti-rabbit (1:50 dilution for 30 minutes ...