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Citations for SouthernBiotech :
101 - 150 of 172 citations for 7 Diethylamino 3 2 thienyl coumarin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... were applied for 2 hours then counterstained with Hoechst and mounted with Fluoromount-G (Southern Biotech). Z-stacks of explants were acquired on an Olympus Spinning Disk confocal microscope with Slidebook software 6.
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bioRxiv - Neuroscience 2024Quote: ... coverslips were mounted onto slides (Fisher 12-544-2) using Fluoromount gold (Southern Biotech 011-01) and dried overnight before storage at -20 °C.
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bioRxiv - Neuroscience 2022Quote: ... A final wash of 3 × 5 minutes was performed prior to mounting using DAPI Fluoromount-G® (Southern Biotech, Cat No. 0100-20). Slices were imaged on a Zeiss LSM 800 laser confocal scan head mounted on a Zeiss Z1Axio Observer inverted microsope frame (Carl Zeiss ...
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bioRxiv - Microbiology 2023Quote: ... Coverslips were then incubated in secondary antibody in blocking buffer for 1 h at RT followed by 3×5 min washes in PBS and then mounted in Fluoromount G with DAPI (Southern Biotech, Birmingham, AL).
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bioRxiv - Immunology 2021Quote: ... Sections were washed in PBS before incubation with secondary antibodies (Table 3) for 1 hr at room temperature followed by mounting with DAPI containing fluoromount (Southern Biotech, Birmingham, AL, USA). Images were captured with an EVOS FL imaging system (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2019Quote: ... They were then washed in PBST (2 x 10 min) and mounted with Dapi Fluoromount-G (SouthernBiotech). The primary antibodies used were ...
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bioRxiv - Immunology 2022Quote: ... Sections were washed and stained with anti-IgA FITC (clone 11-44-2, Southern Biotech, 1:100) in blocking buffer in the dark for 30min at RT ...
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bioRxiv - Immunology 2023Quote: ... Cells were then activated with 20 µg/mL of F(ab’)2 anti-human IgM (Southern Biotech) or left resting ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were counter-stained with DAPI (4′,6-diamidino-2-phenylindole) and mounted with Fluoromount-G (SouthernBiotech).
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bioRxiv - Microbiology 2020Quote: ... Bound antibodies were detected with horseradish peroxidase (HRP)-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech) in 1% BSA in PBS-T and Tetramethylbenzidine (TMB ...
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bioRxiv - Cell Biology 2020Quote: ... Nuclei were stained with 4,6 - diamidino-2 phenylindole (DAPI) and samples were preserved in Fluoromount-G reagent (SouthernBiotech) and kept at 4°C until analyzed ...
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bioRxiv - Developmental Biology 2022Quote: ... the sections were washed with PBS 2 times and mounted with Fluoromount-G medium (Southern Biotech, 0100-01). The immunofluorescence staining was imaged with a laser scanning confocal microscope LSM880 (Carl Zeiss ...
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bioRxiv - Immunology 2019Quote: ... Captured antibodies were detected with secondary R-phycoerythrin-labeled goat-anti-human IgA F(ab’)2 (Southern Biotech) or IgG F(ab’)2 (Jackson Immunoresearch Laboratories) ...
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bioRxiv - Immunology 2020Quote: Model antigens used in solution or on PMS were goat anti-mouse Igκ F(ab’)2 (Southern Biotech) for mouse splenic B cells and goat F(ab’)2 anti-human Fc5μ (Jackson Immunoresearch ...
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bioRxiv - Cancer Biology 2020Quote: ... with 10 μg/mI F(ab’)2 Anti-human anti-IgM-UNLB (#2022-01, Southern Biotech, Birmingham, AL) resuspended in PBS ...
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bioRxiv - Immunology 2023Quote: ... Cells were either left unstimulated or 20 µg/mL of F(ab’)2 anti-human IgM (Southern Biotech) was added directly to the well ...
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bioRxiv - Immunology 2023Quote: ... cells were either left unstimulated or 20 µg/mL of F(ab’)2 anti-human IgM (Southern Biotech) was added directly to the well ...
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bioRxiv - Immunology 2023Quote: ... Cells were either left unstimulated or 20 µg/mL of F(ab’)2 anti-human IgM (Southern Biotech) was added directly to the well ...
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bioRxiv - Immunology 2024Quote: ... Human B cell stimulation used 10ng/mL Goat F(ab’)2 Anti-human IgM (Southern Biotech #2022-01), 2ug/mL of recombinant human sCD40 (invitrogen PHP0025) ...
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bioRxiv - Immunology 2021Quote: ... IgG capture beads were generated by the direct conjugation of goat anti-Human IgG F(ab)2 (Southern Biotech) to array particles (Spherotech ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclei were labeled with 4’,6-Diamidino-2-Phenylindole (DAPI) and slides were mounted using Fluoromount mounting medium (SouthernBioTech). Z-stack photomicrographs of dorsal and ventral lower lumbar spinal cord were acquired at 10x magnification using a Nikon Eclipse Ti2 inverted confocal microscope ...
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bioRxiv - Immunology 2020Quote: ... CMV glycoprotein–specific antibody binding was detected with phycoerythrin-conjugated goat anti-human IgG (2 μg/mL, Southern Biotech). Beads were washed and acquired on a Bio-Plex 200 instrument (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... followed by staining with goat F(ab’)2 anti-human kappa-Alexa Fluor 647 (5 μg/ml, Southern Biotech) (Supporting figure 1A).
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bioRxiv - Immunology 2021Quote: ... RBC-depleted splenocytes were incubated with 1 μg/mL of goat F(ab’)2 anti-mouse Igκ-biotin (SouthernBiotech) for 30 minutes at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were mounted on glass slides with 4’,6-diamidino-2-306 phenylindole (DAPI) Fluoromount-G (Southern Biotech) or Vectashield (Vector Laboratories ...
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bioRxiv - Immunology 2023Quote: ... At various time points after addition of 20 µg/mL of F(ab’)2 anti-human IgM (Southern Biotech), cells were then labeled by addition of hydrogen peroxide (H2O2 ...
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bioRxiv - Microbiology 2023Quote: ... followed by staining with goat F(ab’)2 anti-human kappa-Alexa Fluor 647 (5 μg/mL, Southern Biotech), fixation in PBS 1% formaldehyde and analysis by flow cytometry (BD FACSVerse).
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bioRxiv - Immunology 2023Quote: ... Samples were either left untreated or treated with 20 µg/mL F(ab’)2 anti-human IgM (Southern Biotech). 48 hours later ...
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bioRxiv - Neuroscience 2023Quote: ... then mounted in 10 μl of 4,6-diamidino-2-phenylindole dihydrochloride (DAPI)-containing Fluoromount G (Southern Biotech, #0100-20). Coverslips were analyzed by microscopy using a Leica confocal microscope (Leica TCS SPE laser at 25-50% power ...
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The antibiotic bedaquiline activates host macrophage innate immune resistance to bacterial infectionbioRxiv - Immunology 2019Quote: ... coverslips were set in Fluoromount G medium containing 1 µg/ml 4′,6-diamidino-2-phenylindole (DAPI) (SouthernBiotech, Birmingham, Alabama) on microscope slides ...
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bioRxiv - Immunology 2021Quote: ... Isolated neutrophils were incubated for 30 minutes at room temperature with 20 μg/ml control F(ab’)2 (Southern Biotech) or mouse-anti-SIRL-1 F(ab’)2 (own production) ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were stimulated with buffer (1% FBS/HBSS) or 10 μg/mL goat F(ab’)2 anti-mouse Igκ (SouthernBiotech) and Indo-1 fluorescence (violet vs blue ...
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bioRxiv - Immunology 2023Quote: Biotinylated Goat F(ab)2 anti-human isotype antibodies (anti-human IgM, SouthernBiotech, Cat # 2022-01; anti-human IgA, SouthernBiotech, Cat # 2052-01 ...
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bioRxiv - Neuroscience 2023Quote: ... Coverslips were washed with PBS and mounted with Fluoromount-G with DAPI (4’,6’-diamidino-2-phenylindole dihydrochloride) (cat. no. 0100-20, SouthernBiotech) for nuclear staining ...
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bioRxiv - Microbiology 2023Quote: ... bound S8V1-157 was detected by incubating the microspheres with 2 μg/ml PE-conjugated rat anti-mouse IgG1 (SB77e, SouthernBiotech) for 1 hr at room temperature ...
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bioRxiv - Immunology 2024Quote: ... wells were washed six times with PBS supplemented with 0.1 % Tween20 and incubated for 2 h with 1 μg/mL biotinylated goat α-mouse IgM or IgG1 (SouthernBiotech) at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... IgG-bound DNP-beads were next incubated with 1 µg/mL Alexa Fluor647-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech) in PBS-TH.
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bioRxiv - Microbiology 2020Quote: ... Antibody binding was detected using directly labeled IgG or Alexa Fluor647-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech). Samples were measured using flow cytometry (BD FACSVerse ...
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bioRxiv - Immunology 2019Quote: ... culture supernatants were incubated 2 h at 37°C on wells pre-coated with 5 μg/mL of goat-anti-mouse IgM (Southern Biotech) and saturated with PBS 2% BSA ...
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in calcium-containing HBSS and incubated at room temperature for 5 minutes before activation by anti-IgM F(ab′)2 (Southern Biotech) or VLPs ...
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bioRxiv - Developmental Biology 2022Quote: ... They were washed in blocking buffer 2X for 2 hours and imaged in situ or mounted on microscope slides using Fluoromount-G (Southern Biotech). Immunostaining Analysis ...
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bioRxiv - Immunology 2022Quote: ... IgG bound to the rSARS-CoV-2 S protein was detected using a horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody (Southern Biotech) incubated for 1 hour ± 10 minutes at 24°C ± 2°C ...
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bioRxiv - Immunology 2022Quote: ... a standard curve for mouse isotype was established for each plate by coating 2 columns with 50 μL goat anti-mouse Ig (1 μg/mL in PBS; Southern Biotech) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... plates were coated with 2 µg/mL of goat anti-human kappa or goat anti-human lambda antibodies cross-absorbed against mouse IgG (Southern Biotech) at 4°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... bacteria were incubated with a Goat F(ab’)2 anti-human Kappa-Alexa Fluor 647 (pre-diluted in PBS 0.1%BSA; 2.5 μg/mL, Southern Biotech #2062-31) to detect bound Fab fragments ...
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bioRxiv - Immunology 2019Quote: ... the membrane was re-blocked with 5% BSA in 1x TBST for 2 hours at room temperature before probing with 1:5000 dilution of anti-human IgG-HRP (Southern Biotech) in 5% BSA/1x TBST for 1 hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... or IgG purified from Ggta1+/+ or Ggta1-/- mouse serum (60 µg/mL, PBS 2% BSA, wt/vol, 30 min.) followed by anti-mouse IgG-PE (Southern Biotech #1030-09 ...
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bioRxiv - Immunology 2020Quote: ... 500 ng of F(ab’)2 fragments of α-μ (clone JDC-15; Dako [α-human]; OB1022; Southern Biotech [α-mouse]) and α-CD19 (clone HD37 ...
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bioRxiv - Immunology 2020Quote: ... for detection of αGal and IgG purified from Ggta1+/+ or Ggta1-/- mouse serum (60 µg/mL, PBS 2% BSA, wt/vol, 30 min.) followed by anti-mouse IgG-PE (Southern Biotech #1030-09 ...
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bioRxiv - Microbiology 2021Quote: ... Fab fragments were detected with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech, 1:500). After washing ...