Labshake search
Citations for SouthernBiotech :
101 - 150 of 210 citations for 6 tert Butyl 4 chlorothieno 2 3 d pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... then 2 times with 1X PBS before mounting with Fluoromount-G (Southern Biotech) on slides with a 0.5mm spacer between the slide and coverslip ...
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bioRxiv - Immunology 2023Quote: Biotinylated Goat F(ab)2 anti-human isotype antibodies (anti-human IgM, SouthernBiotech, Cat # 2022-01 ...
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bioRxiv - Immunology 2023Quote: ... that were coated with 2 µg/ml goat anti-human Ig (Southern Biotech) in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... we incubated them with DAPI Fluoromount G overnight at 4°C (Cat# 0100-20; SouthernBiotech, Birmingham, AL). Slices containing biocytin filled astrocytes were fixed ...
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bioRxiv - Developmental Biology 2020Quote: ... stained samples were washed again 3 times with PBS prior to mounting in Fluoromount-G medium (Southern Biotech, 0100-01) for imaging ...
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bioRxiv - Neuroscience 2022Quote: ... then washed 3 x 10 minutes as above before placing in PBS and mounting onto microscope slides with Fluoromount-G (SouthernBiotech). Another antibody combination was used alongside ChAT labelling (rabbit anti-uOR [Abcam [ab10275 ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were rinsed for 3 × 15 min in PBS and cover-slipped with DAPI Fluoromount-G (Cat. Nr. 0100-20, SouthernBiotech). Tissue slices was imaged using a Confocal Microscope (Olympus FV1000 with Fluoview software) ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were then washed with PBS for 3 × 10 min and mounted onto slides with Fluoromount-G (SouthernBiotech, 0100-01). Fluorescent samples were examined with a NIKON A1 + SIM confocal microscope with a 60 × oil objective (CFI Plan Apochromat Lambda ...
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bioRxiv - Neuroscience 2022Quote: ... for 5min and washed 3 times before mounting on glass slides and covered with Fluoromount-G mounting medium (Southern Biotech). Images were acquired on an Olympus slide scanner VS120 L100 with a 20X objective.
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bioRxiv - Cell Biology 2023Quote: ... All coverslips were then washed 3 times in PBS before being mounted on a drop of mounting medium (DAPI fluoromount – G 1:1000; SouthernBiotech)) on labeled glass coverslips ...
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bioRxiv - Developmental Biology 2023Quote: ... slides were washed 2 x for 5 minutes in PBS-tx and 3 x for 5 minutes in PBS and cover slipped with DAPI Fluoromount-G (SouthernBiotech).
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bioRxiv - Neuroscience 2021Quote: ... we incubated the slice with DAPI Fluoromount G overnight at 4°C (Cat# 0100-20; SouthernBiotech, Birmingham, AL) to ensure the whole slice could be visualized under fluorescence microscopy to localize the biocytin-filled ...
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bioRxiv - Immunology 2020Quote: ... additions of either goat F(ab’)2 anti-human IgM LE/AF (Southern Biotech) at a final concentration of 10 µg mL-1 or media were made and the cells incubated for a further 18h at 37°C with 5% CO2.
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bioRxiv - Immunology 2021Quote: ... or equimolar concentrations of the Fab’2 fragments (Southern Biotech; 0.4-100 ug/mL) for 20 hours after which activation was measured using flow cytometry as described above.
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bioRxiv - Cancer Biology 2022Quote: ... Goat F(ab’)2 anti-human IgM was from Southern Biotech (Birmingham, AL, USA). BD Phosflow Fix Buffer I and Perm Buffer III were from BD Biosciences ...
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bioRxiv - Cell Biology 2020Quote: ... and mounting under a coverslip with DAPI Fluoromount-G (Southern Biotech; 1-2 drops). Nuclear staining was detected with excitation at 405 nm ...
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bioRxiv - Microbiology 2023Quote: ... or goat F(ab’)2 anti-human IgM-PE (5 µg/ml, Southern Biotech) for 30 minutes at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Stained retinas were washed 3 times with PBS for 10 mins at room temperature and mounted using Fluoromount-G slide mounting medium (Southern Biotech). Flat Mount Retina Slides were proceeded to image with confocal microscopy (Zeiss).
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bioRxiv - Immunology 2020Quote: ... were incubated with serial 3-fold dilutions of either fecal supernatant (100 µL) or standard IgA (100 µL - SouthernBiotech, Birmingham, USA) for 1h30 at 37°C ...
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bioRxiv - Immunology 2021Quote: ... followed by washing 3 times with PBST and then a 1:20000 dilution of goat anti-rabbit IgG-HRP (Southern Biotech). After washing 3 times with PBST ...
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bioRxiv - Immunology 2020Quote: ... followed by washing 3 times with PBST and then a 1:20000 dilution of goat anti-rabbit IgG-HRP (Southern Biotech). After washing 3 times with PBST ...
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bioRxiv - Neuroscience 2020Quote: ... After thorough washing fluorescent secondary antibodies were applied at room temperature for 3 hours before counterstaining with DAPI and mounting with FluoroMount G (Southern Biotech). Slides were imaged using a Leica SP8 using 20x (NA 0.75 ...
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bioRxiv - Cell Biology 2021Quote: ... Afterward cells were fixed with 4% FA and mounted on glass slides using Fluoromount-G mounting medium (Southern Biotech). Images of the cells were acquired using confocal microscopy and analyzed with ZEN software (Carl Zeiss).
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bioRxiv - Cell Biology 2021Quote: ... Afterward cells were fixed with 4% FA and mounted on glass slides using Fluoromount-G mounting medium (Southern Biotech). Images of the cells were acquired using confocal microscopy and analyzed with ZEN software (Carl Zeiss).
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bioRxiv - Immunology 2019Quote: ... The sections were incubated with monoclonal antibodies (purchased from Southern Biotech, Cambridge, UK, Table 2) specific for various leukocyte subpopulations ...
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bioRxiv - Immunology 2021Quote: ... then incubated for 2 h at room temperature with Streptavidin-alkaline phosphatase from Southern Biotech at 2 μg/mL ...
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bioRxiv - Developmental Biology 2023Quote: FISH-stained embryos and adults were mounted in 2 μL DAPI fluoromount-G (Southern Biotech) with 37.36 μm glass beads (Whitehouse scientific ...
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bioRxiv - Microbiology 2023Quote: ... cells were labeled with 2 μg/ml PE-conjugated goat anti-human IgG (Southern Biotech) for 30 min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... ELISA plates were coated with 2 µg/ml of goat anti-human IgG (Southern Biotech) at 4°C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... Retinas were again washed 3 times for 30 minutes each with PBS before a cover slip was attached with Fluoromount-G (SouthernBiotech, Birmingham, Alabama). For RGC counting ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Slides were then washed in PBS + 0.1% Triton 3 × 10 min and PBS 1 × 5 min then coverslipped with Fluoromount G (Southern biotech Cat OB10001) under glass cover slips ...
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bioRxiv - Microbiology 2020Quote: ... washed 3 times in ice-cold PBSM and then bound antibody detected with goat anti-human kappa-A647 (SouthernBiotech, cat# 2060-31) After washing ...
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bioRxiv - Immunology 2022Quote: ... was added to each well and incubated at RT for 3 minutes and the reaction was terminated with 50 μL of TMB Stop solution (SouthernBiotech, 0412-01). Absorbance at 450 nm (OD450 ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed with PBS (3 x 5 minutes) and mounted on standard microscope glass slides using DAPI-Fluoromount G (SouthernBiotech, 0100-20). Antibodies and dilutions used were as follows ...
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bioRxiv - Immunology 2023Quote: ... plates were washed 3 times and incubated with 50 μl of either 0.25 μg/ml biotinylated anti-mouse IgG1 (Southern Biotech, 1070-08), or biotinylated anti-mouse IgG (H+L ...
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bioRxiv - Cancer Biology 2022Quote: ... the coverslips were washed 3 times with PBS and mounted on microscopy slides using FluoromountG solution containing DAPI (Southern Biotech; 0100-01). Slides were imaged using a Zeiss 880 LSM with Airyscan microscope.
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bioRxiv - Neuroscience 2024Quote: ... Retinas were again washed 3 times for 30 minutes each with PBS before a cover slip was attached with Fluoromount-G (SouthernBiotech, Birmingham, Alabama). Images of immunostained wholemounts were acquired with a Keyence epifluorescence microscope (BZ-X800 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were fixed with 4% paraformaldehyde for 30 min at room temperature and stained with DAPI Fluoromount-G (Southern Biotech) to observe intracellular localization using an Olympus BX63 fluorescence microscope ...
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bioRxiv - Cancer Biology 2023Quote: ... the coverslips were fixed in 4% paraformaldehyde for 20 min at RT and mounted in DAPI Fluoromount-G (SouthernBiotech, USA). Slides were subjected to confocal imaging in a Zeiss LSM 700 microscope (Zeiss ...
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bioRxiv - Neuroscience 2022Quote: ... were applied for 2 hours then counterstained with Hoechst and mounted with Fluoromount-G (Southern Biotech). Z-stacks of explants were acquired on an Olympus Spinning Disk confocal microscope with Slidebook software 6.
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bioRxiv - Neuroscience 2024Quote: ... coverslips were mounted onto slides (Fisher 12-544-2) using Fluoromount gold (Southern Biotech 011-01) and dried overnight before storage at -20 °C.
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bioRxiv - Neuroscience 2022Quote: ... A final wash of 3 × 5 minutes was performed prior to mounting using DAPI Fluoromount-G® (Southern Biotech, Cat No. 0100-20). Slices were imaged on a Zeiss LSM 800 laser confocal scan head mounted on a Zeiss Z1Axio Observer inverted microsope frame (Carl Zeiss ...
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bioRxiv - Microbiology 2023Quote: ... Coverslips were then incubated in secondary antibody in blocking buffer for 1 h at RT followed by 3×5 min washes in PBS and then mounted in Fluoromount G with DAPI (Southern Biotech, Birmingham, AL).
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bioRxiv - Neuroscience 2022Quote: ... Slices were stored at 4°C until mounting on non-gelatin-coated slides followed by cover of DAPI fluoromount (Southern Biotech) and cover-slipped ...
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bioRxiv - Immunology 2020Quote: ... plates were washed 4 times with PBS-T and an anti-monkey IgG conjugated to horseradish peroxidase (Southern Biotech 4700-5) 1 hour at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... labeled protein was visualized using the AP substrate 5-bromo-4-chloro-indolyl-phosphate/nitro blue tetrazolium (BCIP/NBT; Southern Biotech).
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bioRxiv - Immunology 2023Quote: ... and incubated with pleural lavage fluid overnight at 4°C prior to detection of bound IgM with a goat anti-mouse IgM-HRP (1020-05 Southern Biotech) for 1h at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Sections were washed in PBS before incubation with secondary antibodies or labelled streptavidin (Supplementary Table 4) for 1hr at room temperature followed by mounting with DAPI containing fluoromount (Southern Biotech). Small to medium sized airways in lung sections were captured by an investigator blind to sample identity with an EVOS FL imaging system (Thermo) ...
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bioRxiv - Microbiology 2023Quote: ... 4°C) and incubated with goat anti-human IgG-Alexa Fluor 647 and goat anti-human IgM-PE (both Southern Biotech) for 30 minutes at 4°C at 600 rpm ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the embryos were stained with 10nM DAPI in 70% glycerol in 1X PBS overnight at 4°C protected from light, washed in 1X SSCT (10 min/wash, twice) before mounting with Fluoromount G (Southern Biotech) on glass bottom dishes (Cellvis ...