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Citations for SouthernBiotech :
101 - 150 of 210 citations for 6 Ethyl 1H indole 2 3 dione 3 O 4 4 4 trifluoro butyl oxime since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... Stained retinas were washed 3 times with PBS for 10 mins at room temperature and mounted using Fluoromount-G slide mounting medium (Southern Biotech). Flat Mount Retina Slides were proceeded to image with confocal microscopy (Zeiss).
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bioRxiv - Immunology 2020Quote: ... were incubated with serial 3-fold dilutions of either fecal supernatant (100 µL) or standard IgA (100 µL - SouthernBiotech, Birmingham, USA) for 1h30 at 37°C ...
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bioRxiv - Immunology 2021Quote: ... followed by washing 3 times with PBST and then a 1:20000 dilution of goat anti-rabbit IgG-HRP (Southern Biotech). After washing 3 times with PBST ...
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bioRxiv - Immunology 2020Quote: ... followed by washing 3 times with PBST and then a 1:20000 dilution of goat anti-rabbit IgG-HRP (Southern Biotech). After washing 3 times with PBST ...
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bioRxiv - Neuroscience 2020Quote: ... After thorough washing fluorescent secondary antibodies were applied at room temperature for 3 hours before counterstaining with DAPI and mounting with FluoroMount G (Southern Biotech). Slides were imaged using a Leica SP8 using 20x (NA 0.75 ...
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bioRxiv - Neuroscience 2022Quote: ... Retinas were again washed 3 times for 30 minutes each with PBS before a cover slip was attached with Fluoromount-G (SouthernBiotech, Birmingham, Alabama). For RGC counting ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Slides were then washed in PBS + 0.1% Triton 3 × 10 min and PBS 1 × 5 min then coverslipped with Fluoromount G (Southern biotech Cat OB10001) under glass cover slips ...
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bioRxiv - Microbiology 2020Quote: ... washed 3 times in ice-cold PBSM and then bound antibody detected with goat anti-human kappa-A647 (SouthernBiotech, cat# 2060-31) After washing ...
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bioRxiv - Immunology 2022Quote: ... was added to each well and incubated at RT for 3 minutes and the reaction was terminated with 50 μL of TMB Stop solution (SouthernBiotech, 0412-01). Absorbance at 450 nm (OD450 ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed with PBS (3 x 5 minutes) and mounted on standard microscope glass slides using DAPI-Fluoromount G (SouthernBiotech, 0100-20). Antibodies and dilutions used were as follows ...
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bioRxiv - Immunology 2023Quote: ... plates were washed 3 times and incubated with 50 μl of either 0.25 μg/ml biotinylated anti-mouse IgG1 (Southern Biotech, 1070-08), or biotinylated anti-mouse IgG (H+L ...
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bioRxiv - Cancer Biology 2022Quote: ... the coverslips were washed 3 times with PBS and mounted on microscopy slides using FluoromountG solution containing DAPI (Southern Biotech; 0100-01). Slides were imaged using a Zeiss 880 LSM with Airyscan microscope.
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bioRxiv - Neuroscience 2024Quote: ... Retinas were again washed 3 times for 30 minutes each with PBS before a cover slip was attached with Fluoromount-G (SouthernBiotech, Birmingham, Alabama). Images of immunostained wholemounts were acquired with a Keyence epifluorescence microscope (BZ-X800 ...
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bioRxiv - Neuroscience 2022Quote: ... A final wash of 3 × 5 minutes was performed prior to mounting using DAPI Fluoromount-G® (Southern Biotech, Cat No. 0100-20). Slices were imaged on a Zeiss LSM 800 laser confocal scan head mounted on a Zeiss Z1Axio Observer inverted microsope frame (Carl Zeiss ...
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bioRxiv - Microbiology 2023Quote: ... Coverslips were then incubated in secondary antibody in blocking buffer for 1 h at RT followed by 3×5 min washes in PBS and then mounted in Fluoromount G with DAPI (Southern Biotech, Birmingham, AL).
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bioRxiv - Immunology 2021Quote: ... Sections were washed in PBS before incubation with secondary antibodies (Table 3) for 1 hr at room temperature followed by mounting with DAPI containing fluoromount (Southern Biotech, Birmingham, AL, USA). Images were captured with an EVOS FL imaging system (ThermoFisher Scientific) ...
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bioRxiv - Bioengineering 2021Quote: ... wells were washed 4x with PBS-T and incubated for 1h at room tepmerature either with HRP-IgG1 (SouthernBiotech 1070-05) (1:4,000 ...
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bioRxiv - Immunology 2023Quote: ... was added to the wells for 1h followed by washing and incubation with horseradish peroxidase-linked goat anti-mouse IgG secondary antibody (Southern Biotech). The avidity index was calculated as ...
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bioRxiv - Molecular Biology 2021Quote: ... Coverslips were then mounted upside-down onto slides with 6 μL Fluoromount G (Southern Biotech) and sealed with nail polish ...
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bioRxiv - Immunology 2021Quote: ... IgA (Clone 11-44-2, Southern Biotech), and IgM (Clone RMM-1 ...
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bioRxiv - Microbiology 2021Quote: ... The plates were washed a second time and incubated for 2 h with Streptavidin-alkaline phosphatase from Southern Biotech (2 µg/mL). The final wash was followed by the addition of Nitro-blue Tetrazolium Chloride/5-bromo-4-chloro 3 ‘indolyphosphate p-toludine salt (NBT/BCIP chromagen ...
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bioRxiv - Immunology 2024Quote: ... goat F(ab′)2 anti-human kappa FITC and goat F(ab′)2 anti-human lambda FITC (SouthernBiotech; to detect antibody expression), and PI (Invitrogen ...
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bioRxiv - Immunology 2021Quote: Goat F(ab’)2 anti-mouse Igκ (Southern Biotech) was biotinylated with 20-fold molar excess of NHS-LC-LC-biotin (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... (2) block sections in 5% normal goat serum (SouthernBiotech) and 0.3% Triton-X solution in PBS for 1h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... goat F(ab’)2 anti-mouse IgM (αIgM, SouthernBiotech) or rat anti-mouse CD40 (αCD40 ...
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bioRxiv - Neuroscience 2020Quote: ... slides were washed with two exchanges of PBS (6-8 min each) and cover slipped with fluoromount-G (Southern Biotech), or Vectashield (Vector labs) ...
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bioRxiv - Cancer Biology 2020Quote: Primary CLL samples were treated with 20 µg/ml anti-IgM F(ab’)2 or control F(ab’)2 (both Southern Biotech, Cambridge Biosciences, UK) for 5 minutes at 37°C/5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... F(ab’)2 fragments or human IgG1 (control) (Southern Biotech) for 1hr at 37°C in a 96-well plate ...
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bioRxiv - Immunology 2022Quote: ... anti-IgG F(ab’)2 (5 μg/mL; Southern Biotech) or anti-IgM F(ab’)2 (5 μg/mL ...
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bioRxiv - Microbiology 2022Quote: ... α-IgA (mAb 11-44-2, FITC, SouthernBiotech catalog no. 1165-02). Cells were stained with primary antibodies (1:50 dilution ...
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bioRxiv - Immunology 2022Quote: ... or anti-IgM F(ab’)2 (5 μg/mL; Southern Biotech). Cells were immediately fixed with pre-warmed paraformaldehyde (4% final concentration) ...
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bioRxiv - Bioengineering 2019Quote: ... for 2 hr and mounted with Fluoromount-G (SouthernBiotech; 0100-20). Imaging was performed on a Nikon A1Rsi confocal microscope.
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bioRxiv - Immunology 2021Quote: ... followed by goat F(ab’)2 anti-mouse IgG1-FITC (Southern Biotech). The percentage of living HUCCT1 cells was estimated by flow cytometry ...
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bioRxiv - Immunology 2020Quote: ... Cells were stimulated with anti-mouse IgM-F(ab)2 (Southern Biotech), ionomycin (4μM ...
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bioRxiv - Microbiology 2022Quote: ... or 2 μg/ml goat anti-hu-IgM-FITC (2020-02, SouthernBiotech) for 30 minutes at 4 °C while shaking ...
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bioRxiv - Immunology 2023Quote: ... 20 µg/mL of F(ab’)2 anti-human IgM (Southern Biotech) was added to the cell medium and incubated for the indicated length of time ...
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bioRxiv - Immunology 2024Quote: ... goat F(ab′)2 anti-human IgG PE (SouthernBiotech, dilution 1:100), and PI (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... goat F(ab’)2 anti-mouse IgG-PE (Southern Biotech, Birmingham, AL); and anti-phospho-AKT (Ser473) ...
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bioRxiv - Immunology 2020Quote: ... washed 6 times and then incubated with 1: 6000 dilution of horseradish peroxidase (HRP) conjugated anti-mouse IgG secondary antibody (Southern Biotech; Birmingham, AL, USA) for 1 h at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... Slides were mounted with DAPI Fluoromount-G (DAPI, 4,6-diamidino-2-phenylindole) (SouthernBiotech). Stained slides were imaged using Leica Application Suite X software on a Leica Sp8 (Leica) ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were mounted using 4,6-diamidino-2-phenylindole (DAPI) Fluoromount-G (SouthernBiotech) and sealed with a coverslip ...
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bioRxiv - Neuroscience 2022Quote: ... then 2 times with 1X PBS before mounting with Fluoromount-G (Southern Biotech) on slides with a 0.5mm spacer between the slide and coverslip ...
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bioRxiv - Immunology 2023Quote: Biotinylated Goat F(ab)2 anti-human isotype antibodies (anti-human IgM, SouthernBiotech, Cat # 2022-01 ...
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bioRxiv - Immunology 2023Quote: ... that were coated with 2 µg/ml goat anti-human Ig (Southern Biotech) in PBS ...
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bioRxiv - Immunology 2020Quote: ... additions of either goat F(ab’)2 anti-human IgM LE/AF (Southern Biotech) at a final concentration of 10 µg mL-1 or media were made and the cells incubated for a further 18h at 37°C with 5% CO2.
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bioRxiv - Immunology 2021Quote: ... or equimolar concentrations of the Fab’2 fragments (Southern Biotech; 0.4-100 ug/mL) for 20 hours after which activation was measured using flow cytometry as described above.
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bioRxiv - Cancer Biology 2022Quote: ... Goat F(ab’)2 anti-human IgM was from Southern Biotech (Birmingham, AL, USA). BD Phosflow Fix Buffer I and Perm Buffer III were from BD Biosciences ...
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bioRxiv - Cell Biology 2020Quote: ... and mounting under a coverslip with DAPI Fluoromount-G (Southern Biotech; 1-2 drops). Nuclear staining was detected with excitation at 405 nm ...
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bioRxiv - Microbiology 2023Quote: ... or goat F(ab’)2 anti-human IgM-PE (5 µg/ml, Southern Biotech) for 30 minutes at 4°C ...
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bioRxiv - Immunology 2019Quote: ... The sections were incubated with monoclonal antibodies (purchased from Southern Biotech, Cambridge, UK, Table 2) specific for various leukocyte subpopulations ...