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Citations for SouthernBiotech :
101 - 150 of 194 citations for 6 Chloro 5 fluoro 1H indole 2 carboxylic acid ethyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... were applied for 2 hours then counterstained with Hoechst and mounted with Fluoromount-G (Southern Biotech). Z-stacks of explants were acquired on an Olympus Spinning Disk confocal microscope with Slidebook software 6.
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bioRxiv - Immunology 2023Quote: ... 3 µg/ml recombinant human TG2 or 2 µg/ml goat anti-human Ig (Southern Biotech) was coated in PBS followed by incubation with culture supernatants diluted in PBS supplemented with 0.1% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... coverslips were mounted onto slides (Fisher 12-544-2) using Fluoromount gold (Southern Biotech 011-01) and dried overnight before storage at -20 °C.
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bioRxiv - Immunology 2020Quote: ... coated with 100 µL/well of goat anti-mouse IgA (5 µg/mL Bic 0.1M, pH 9.6; Southern Biotech) were incubated with serial 3-fold dilutions of either fecal supernatant (100 µL ...
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bioRxiv - Neuroscience 2019Quote: ... They were then washed in PBST (2 x 10 min) and mounted with Dapi Fluoromount-G (SouthernBiotech). The primary antibodies used were ...
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bioRxiv - Immunology 2022Quote: ... Sections were washed and stained with anti-IgA FITC (clone 11-44-2, Southern Biotech, 1:100) in blocking buffer in the dark for 30min at RT ...
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bioRxiv - Immunology 2023Quote: ... Cells were then activated with 20 µg/mL of F(ab’)2 anti-human IgM (Southern Biotech) or left resting ...
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bioRxiv - Developmental Biology 2021Quote: ... The sections were then washed three times for 5 min with PBST and slides were mounted with Fluoromount-G containing DAPI and photobleaching inhibitors (SouthernBiotech). All steps were performed in a dark humid chamber to prevent evaporation and photobleaching ...
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bioRxiv - Cell Biology 2021Quote: ... coverslips were washed in PEM supplemented with 0.01% Triton-X-100 for 5 minutes before mounting on clean slides using DAPI Fluoromount G (Southern Biotech) or Prolong Gold.
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bioRxiv - Immunology 2021Quote: ... at 1×106 cells/well and stimulated for 48 hours with 5 μg/mL of RBD-His or hCD81-His or media alone (RPMI + 5% FCS) in the presence of anti-mouse CD28 antibody [0,5 μg/ml] (Southern Biotech). As positive control cells were stimulated with anti-mouse CD3 [0,05 μg/ml] (Southern Biotech) ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 5 times with PBS-T and incubated with goat anti-mouse IgG1 antibody conjugated with horseradish peroxidase (SouthernBiotech) for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... slides were washed 2 x for 5 minutes in PBS-tx and 3 x for 5 minutes in PBS and cover slipped with DAPI Fluoromount-G (SouthernBiotech).
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed three times in 1X PBS for 5 min before being mounted on slides with Fluoromount-G (SouthernBiotech).
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed with PBS-T for 5 mins at room temperature and then mounted with DAPI-Fluoromount-G (SouthernBiotech) to adhere coverslips ...
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bioRxiv - Immunology 2024Quote: ... Membranes were incubated in TBST containing 5% (w/v) skimmed milk with goat anti-mouse IgG conjugated to horseradish peroxidase (HRP) (1:2000, 1010-05, SouthernBiotech) for 3h at 4°C in a rotary platform and washed thrice with TBST for 10 min each time ...
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bioRxiv - Microbiology 2020Quote: ... Bound antibodies were detected with horseradish peroxidase (HRP)-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech) in 1% BSA in PBS-T and Tetramethylbenzidine (TMB ...
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bioRxiv - Cell Biology 2020Quote: ... Nuclei were stained with 4,6 - diamidino-2 phenylindole (DAPI) and samples were preserved in Fluoromount-G reagent (SouthernBiotech) and kept at 4°C until analyzed ...
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bioRxiv - Developmental Biology 2022Quote: ... the sections were washed with PBS 2 times and mounted with Fluoromount-G medium (Southern Biotech, 0100-01). The immunofluorescence staining was imaged with a laser scanning confocal microscope LSM880 (Carl Zeiss ...
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bioRxiv - Immunology 2019Quote: ... Captured antibodies were detected with secondary R-phycoerythrin-labeled goat-anti-human IgA F(ab’)2 (Southern Biotech) or IgG F(ab’)2 (Jackson Immunoresearch Laboratories) ...
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bioRxiv - Immunology 2020Quote: Model antigens used in solution or on PMS were goat anti-mouse Igκ F(ab’)2 (Southern Biotech) for mouse splenic B cells and goat F(ab’)2 anti-human Fc5μ (Jackson Immunoresearch ...
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bioRxiv - Cancer Biology 2020Quote: ... with 10 μg/mI F(ab’)2 Anti-human anti-IgM-UNLB (#2022-01, Southern Biotech, Birmingham, AL) resuspended in PBS ...
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bioRxiv - Immunology 2023Quote: ... Cells were either left unstimulated or 20 µg/mL of F(ab’)2 anti-human IgM (Southern Biotech) was added directly to the well ...
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bioRxiv - Immunology 2023Quote: ... cells were either left unstimulated or 20 µg/mL of F(ab’)2 anti-human IgM (Southern Biotech) was added directly to the well ...
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bioRxiv - Immunology 2023Quote: ... Cells were either left unstimulated or 20 µg/mL of F(ab’)2 anti-human IgM (Southern Biotech) was added directly to the well ...
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bioRxiv - Immunology 2024Quote: ... Human B cell stimulation used 10ng/mL Goat F(ab’)2 Anti-human IgM (Southern Biotech #2022-01), 2ug/mL of recombinant human sCD40 (invitrogen PHP0025) ...
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bioRxiv - Immunology 2019Quote: ... Sera were incubated 2 h at 37°C on wells pre-coated with 5 μg/mL of NP15-BSA or goat-anti-mouse IgM (Southern Biotech) and saturated with PBS 2% BSA ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were then rinsed again three to four times in PBS for 5-10 min and mounted on Fisherbrand microscope slides and cover slipped using Fluoromount-G (Southern Biotech) mounting medium.
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bioRxiv - Neuroscience 2020Quote: ... All coverslips were then washed three times (five minutes per wash) in 1xPBS followed by block and permeabilization with 5% (v/v) normal goat serum (NGS, Southern Biotech) and 0.25% (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were subsequently washed three times for 5 min each in PBS and mounted on microscope slides (Brain Research Laboratories) using Fluoromount-G mounting medium (Southern Biotech). Images were obtained with an EVO FL Imaging System equipped with 4x objective to visualize entire ACA with the adjacent cortical areas simultaneously ...
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bioRxiv - Physiology 2020Quote: ... Sections were again washed 3 times 5 minutes with 1x PBS followed by mounting with coverslips using Fluoromount-G mounting media containing DAPI nuclear stain (Southern Biotech).
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bioRxiv - Immunology 2020Quote: ... plates were washed 4 times with PBS-T and an anti-monkey IgG conjugated to horseradish peroxidase (Southern Biotech 4700-5) 1 hour at 37°C ...
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bioRxiv - Immunology 2022Quote: ... NP-OVA specific IgG1 was detected as previously described48 using plates were coated with TNP(5)-BSA and detected using goat anti-mouse IgG1-HRP (Southern Biotech).
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bioRxiv - Physiology 2023Quote: ... Non-specific antibody binding was blocked for 1 hour at room temperature with a blocking solution containing: 5% normal goat serum (006001, SouthernBiotech™), 0.3% Triton™ X-100 (X100 ...
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bioRxiv - Microbiology 2023Quote: ... Alexa Fluor® Plus 488 secondary antibodies were diluted 1:1,000 in the respective blocking solutions before mounting with 5 µL DAPI Fluoromount-G® (Southern Biotech) and imaging ...
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bioRxiv - Immunology 2021Quote: ... IgG capture beads were generated by the direct conjugation of goat anti-Human IgG F(ab)2 (Southern Biotech) to array particles (Spherotech ...
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bioRxiv - Immunology 2020Quote: ... CMV glycoprotein–specific antibody binding was detected with phycoerythrin-conjugated goat anti-human IgG (2 μg/mL, Southern Biotech). Beads were washed and acquired on a Bio-Plex 200 instrument (Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: ... RBC-depleted splenocytes were incubated with 1 μg/mL of goat F(ab’)2 anti-mouse Igκ-biotin (SouthernBiotech) for 30 minutes at 4°C ...
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bioRxiv - Immunology 2023Quote: ... At various time points after addition of 20 µg/mL of F(ab’)2 anti-human IgM (Southern Biotech), cells were then labeled by addition of hydrogen peroxide (H2O2 ...
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bioRxiv - Immunology 2023Quote: ... Samples were either left untreated or treated with 20 µg/mL F(ab’)2 anti-human IgM (Southern Biotech). 48 hours later ...
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bioRxiv - Neuroscience 2023Quote: ... then mounted in 10 μl of 4,6-diamidino-2-phenylindole dihydrochloride (DAPI)-containing Fluoromount G (Southern Biotech, #0100-20). Coverslips were analyzed by microscopy using a Leica confocal microscope (Leica TCS SPE laser at 25-50% power ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Slides were then washed in PBS + 0.1% Triton 3 × 10 min and PBS 1 × 5 min then coverslipped with Fluoromount G (Southern biotech Cat OB10001) under glass cover slips ...
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bioRxiv - Cancer Biology 2020Quote: ... All cells were maintained at 37 ◻C with 5% CO2 and were mycoplasma free as determined a PCR assay (Southern Biotech).
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bioRxiv - Biochemistry 2020Quote: ... for 1 min, washed with PBS again (3X, 5 min) and slides were mounted with Fluoromount-G mounting medium (SouthernBiotech, Birmingham, AL). Images were acquired on a Leica SP5 confocal microscope and analyzed by Fiji ImageJ.
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bioRxiv - Microbiology 2023Quote: ... cells were attached to a poly-L-lysine treated slide before 5 μl of Fluoromount G with DAPI (Cambridge Bioscience, Southern Biotech) was added and coverslip applied ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... at the level of injection (L3-5) as well as slices from corresponding DRGs (20 µm thickness) were directly mounted with DAPI Fluoromount-G® (SouthernBiotech™) and imaged using Axio Scan Z1 ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed with PBS (3 x 5 minutes) and mounted on standard microscope glass slides using DAPI-Fluoromount G (SouthernBiotech, 0100-20). Antibodies and dilutions used were as follows ...
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bioRxiv - Immunology 2021Quote: ... Isolated neutrophils were incubated for 30 minutes at room temperature with 20 μg/ml control F(ab’)2 (Southern Biotech) or mouse-anti-SIRL-1 F(ab’)2 (own production) ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were stimulated with buffer (1% FBS/HBSS) or 10 μg/mL goat F(ab’)2 anti-mouse Igκ (SouthernBiotech) and Indo-1 fluorescence (violet vs blue ...
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bioRxiv - Immunology 2023Quote: Biotinylated Goat F(ab)2 anti-human isotype antibodies (anti-human IgM, SouthernBiotech, Cat # 2022-01; anti-human IgA, SouthernBiotech, Cat # 2052-01 ...
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bioRxiv - Microbiology 2023Quote: ... bound S8V1-157 was detected by incubating the microspheres with 2 μg/ml PE-conjugated rat anti-mouse IgG1 (SB77e, SouthernBiotech) for 1 hr at room temperature ...