Labshake search
Citations for SouthernBiotech :
101 - 150 of 544 citations for 6 Bromo 1 2 cyanoethyl 2 4 diethylamino phenyl benz cd indolium chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... cells were resuspended in calcium-containing HBSS and incubated at room temperature for 5 minutes before activation by anti-IgM F(ab′)2 (Southern Biotech) or VLPs ...
-
bioRxiv - Developmental Biology 2022Quote: ... They were washed in blocking buffer 2X for 2 hours and imaged in situ or mounted on microscope slides using Fluoromount-G (Southern Biotech). Immunostaining Analysis ...
-
bioRxiv - Immunology 2022Quote: ... IgG bound to the rSARS-CoV-2 S protein was detected using a horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody (Southern Biotech) incubated for 1 hour ± 10 minutes at 24°C ± 2°C ...
-
bioRxiv - Immunology 2020Quote: ... plates were coated with 2 µg/mL of goat anti-human kappa or goat anti-human lambda antibodies cross-absorbed against mouse IgG (Southern Biotech) at 4°C overnight ...
-
bioRxiv - Microbiology 2022Quote: ... bacteria were incubated with a Goat F(ab’)2 anti-human Kappa-Alexa Fluor 647 (pre-diluted in PBS 0.1%BSA; 2.5 μg/mL, Southern Biotech #2062-31) to detect bound Fab fragments ...
-
bioRxiv - Immunology 2020Quote: ... or IgG purified from Ggta1+/+ or Ggta1-/- mouse serum (60 µg/mL, PBS 2% BSA, wt/vol, 30 min.) followed by anti-mouse IgG-PE (Southern Biotech #1030-09 ...
-
bioRxiv - Immunology 2020Quote: ... 500 ng of F(ab’)2 fragments of α-μ (clone JDC-15; Dako [α-human]; OB1022; Southern Biotech [α-mouse]) and α-CD19 (clone HD37 ...
-
bioRxiv - Immunology 2020Quote: ... for detection of αGal and IgG purified from Ggta1+/+ or Ggta1-/- mouse serum (60 µg/mL, PBS 2% BSA, wt/vol, 30 min.) followed by anti-mouse IgG-PE (Southern Biotech #1030-09 ...
-
bioRxiv - Immunology 2021Quote: ... B cells were then cultured for a further 2 days in RPMI (as described above) or supplemented with 500ng/ml Igκ (Southern Biotech). Where indicated the following reagents were added into in vitro cultures ...
-
bioRxiv - Bioengineering 2021Quote: ... arrays were incubated for an additional 2 h at RT and 60 rpm with goat anti-mouse IgG conjugated to HRP (Southern Biotech) diluted 1:5000 in blocking solution ...
-
bioRxiv - Cell Biology 2020Quote: ... localization of antibody labeling was detected after a 1 hr incubation with secondary antibody diluted in PBS containing 2% normal goat serum and mounting under a coverslip with DAPI Fluoromount-G (Southern Biotech). PPCs were visualized by fluorescent microscopy and their numbers were assessed qualitatively by visual examination ...
-
bioRxiv - Biochemistry 2020Quote: ... The immunoreactivity was detected by incubation with Rabbit F(ab’)2 Anti-Goat IgG(H+L)-HRP (6020-05, Southern Biotech) diluted 3000 × in PBS-T.
-
bioRxiv - Immunology 2024Quote: ... membranes were incubated with primary antibodies overnight at 4 °C and during 2 hours at room temperature with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit IgG (SouthernBiotech, USA). Membranes were then revealed using G:BOX Chemi XL1.4 Fluorescent & Chemiluminescent Imaging System (Syngene ...
-
bioRxiv - Cancer Biology 2024Quote: ... permeabilized with 0.1% Triton X-100 (Fisher Biotech, Hampton, NH, USA), and blocked with PBS supplemented with goat serum (2%, v/v) (Southern biotech) and 0.1% Tween-20 (Thermo Fisher) ...
-
bioRxiv - Molecular Biology 2020Quote: ... and then 4 × 1 hour prior to counterstaining with the nuclear marker (DAPI) (1 ng/ml) and mounting in Fluoromount G (Southern Biotech, Birmingham, AL). The primary antibody used was anti-Schistosoma japonicum Ago2 (clone 650-1-1-KM864-3-11E8 ...
-
bioRxiv - Cell Biology 2022Quote: ... Coverslips with stained cells were washed 2 x 5 min in PBS and then mounted with Fluoromount-G (Southern Biotech 0100-01) for image acquisition ...
-
bioRxiv - Developmental Biology 2019Quote: ... A 1 μg/μl 4’6-diamidino-2-phenylindole (DAPI) solution was applied to the slides for nuclear staining prior to mounting in Fluoromount-G (Southern Biotech, 0100-01) with 34×60 mm coverslips (VWR ...
-
bioRxiv - Immunology 2022Quote: ... ELISA plates were coated with 1 μg/ml recombinant SARS-CoV-2 S protein (43) and serially diluted serum samples were added and detected via anti-monkey IgG-HRP (Southern Biotech, Birmingham, AL). Plates were developed using a TMB substrate (source ...
-
bioRxiv - Immunology 2020Quote: ... washed 6 times and then incubated with 1: 6000 dilution of horseradish peroxidase (HRP) conjugated anti-mouse IgG secondary antibody (Southern Biotech; Birmingham, AL, USA) for 1 h at RT ...
-
bioRxiv - Immunology 2022Quote: ... plates were washed 4× with ELISA wash buffer and a 1:4000 dilution of goat anti-mouse IgG (SouthernBiotech, catalog #1030-05) in ELISA blocking buffer was added to each well and incubated at 37°C for 1 hour followed by 4 washes with ELISA wash buffer ...
-
bioRxiv - Microbiology 2023Quote: ... 1×109 bacteria were washed with 1 ml ice-cold PBS and stained for 30 min at 4°C with 4 µg of FITC-conjugated anti-mouse IgA antibody (Southern Biotech) in 100 µl of staining buffer ...
-
bioRxiv - Molecular Biology 2021Quote: ... Coverslips were then mounted upside-down onto slides with 6 μL Fluoromount G (Southern Biotech) and sealed with nail polish ...
-
bioRxiv - Immunology 2019Quote: ... 96-well Immulon 4 HBX plates were coated overnight at 4°C with 5 mg/ml polyclonal goat-anti mouse κ (Southern Biotech; 1050-01). Nonspecific binding was blocked with 1% bovine serum albumin in PBS and samples were incubated at 37°C ...
-
bioRxiv - Genomics 2024Quote: ... incubating overnight at 4°C in DAPI fluoromount-G (Southern Biotech) and then mounting and imaging on a confocal laser microscope (Leica Sp8).
-
bioRxiv - Immunology 2019Quote: ... were coated overnight at 4°C with goat anti-mouse Ig (SouthernBiotech). After blocking with 2% BSA ...
-
bioRxiv - Cell Biology 2020Quote: ... coverslips were mounted with 4–6μL of Fluoromount G (SouthernBiotech, 0100-01) on slides (KNITTEL Starfrost ...
-
bioRxiv - Neuroscience 2020Quote: ... slides were washed with two exchanges of PBS (6-8 min each) and cover slipped with fluoromount-G (Southern Biotech), or Vectashield (Vector labs) ...
-
bioRxiv - Developmental Biology 2023Quote: ... post-fixed in 4% paraformaldehyde and coverslipped with DAPI-Fluoromount® G (SouthernBiotech). Third-generation mRNA ISH by chain reaction (HCR ...
-
bioRxiv - Developmental Biology 2023Quote: ... post-fixed in 4% paraformaldehyde and coverslipped with DAPI-Fluoromount® G (SouthernBiotech), while wholemounts were rinsed ...
-
bioRxiv - Immunology 2021Quote: ... The reaction was visualized by subsequent addition of 2,2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) substrate (Southern Biotech, #0202-01).
-
bioRxiv - Immunology 2021Quote: ... goat anti-mouse IgM (4 μg/ml; Southern Biotech, Cat#1020-05; RRID: AB_2794201) or goat anti-mouse IgA (4 μg/ml ...
-
bioRxiv - Immunology 2021Quote: ... or goat anti-mouse IgA (4 μg/ml; Southern Biotech, Cat#1040-05; RRID: AB_2714213). The reaction was visualized by subsequent addition of 2,2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were washed and incubated at 4°C with goat anti-mouse Ig-PE (SouthernBiotech). Cells were washed and mean fluorescence intensity (MFI ...
-
bioRxiv - Pathology 2024Quote: ... or anti-type I collagen at 4 µg/ml (Southern Biotech 1310-01, Birmingham, AL) using heat-induced epitope retrieval at basic pH ...
-
bioRxiv - Developmental Biology 2021Quote: ... Sections were washed again 4 times for 20 min before mounting in Fluoromount-G (Southern Biotech).
-
bioRxiv - Microbiology 2023Quote: ... were coated overnight at 4°C with polyclonal goat anti-human IgA (Southern Biotech, #C5213-R466) (2 μg/ml ...
-
bioRxiv - Neuroscience 2019Quote: ... we incubated them with DAPI Fluoromount G overnight at 4°C (Cat# 0100-20; SouthernBiotech, Birmingham, AL). Slices containing biocytin filled astrocytes were fixed ...
-
bioRxiv - Neuroscience 2021Quote: ... we incubated the slice with DAPI Fluoromount G overnight at 4°C (Cat# 0100-20; SouthernBiotech, Birmingham, AL) to ensure the whole slice could be visualized under fluorescence microscopy to localize the biocytin-filled ...
-
bioRxiv - Pathology 2022Quote: ... anti-collagen 1 (1:200, Southern Biotech, 1310-01), anti-collagen 4 (1:5000 ...
-
bioRxiv - Cell Biology 2021Quote: ... Afterward cells were fixed with 4% FA and mounted on glass slides using Fluoromount-G mounting medium (Southern Biotech). Images of the cells were acquired using confocal microscopy and analyzed with ZEN software (Carl Zeiss).
-
bioRxiv - Cell Biology 2021Quote: ... Afterward cells were fixed with 4% FA and mounted on glass slides using Fluoromount-G mounting medium (Southern Biotech). Images of the cells were acquired using confocal microscopy and analyzed with ZEN software (Carl Zeiss).
-
bioRxiv - Molecular Biology 2023Quote: ... cells were fixed with 4% paraformaldehyde for 30 min at room temperature and stained with DAPI Fluoromount-G (Southern Biotech) to observe intracellular localization using an Olympus BX63 fluorescence microscope ...
-
bioRxiv - Cancer Biology 2023Quote: ... the coverslips were fixed in 4% paraformaldehyde for 20 min at RT and mounted in DAPI Fluoromount-G (SouthernBiotech, USA). Slides were subjected to confocal imaging in a Zeiss LSM 700 microscope (Zeiss ...
-
bioRxiv - Neuroscience 2021Quote: ... or a 1:1 mix of anti-rabbit (Southern Biotech, OB405005) and antirabbit (Cell Signaling ...
-
bioRxiv - Molecular Biology 2020Quote: ... or a 1:1 mix of anti-rabbit (Southern Biotech, OB405005) and anti-rabbit (Cell Signaling ...
-
bioRxiv - Immunology 2023Quote: ... Goat Monoclonal anti-mouse Collagen Type 1 (1:200, Southern Biotech), Rat Monoclonal anti-mouse CD326 (G8.8 ...
-
bioRxiv - Neuroscience 2022Quote: ... Slices were stored at 4°C until mounting on non-gelatin-coated slides followed by cover of DAPI fluoromount (Southern Biotech) and cover-slipped ...
-
bioRxiv - Immunology 2020Quote: ... plates were washed 4 times with PBS-T and an anti-monkey IgG conjugated to horseradish peroxidase (Southern Biotech 4700-5) 1 hour at 37°C ...
-
bioRxiv - Immunology 2023Quote: ... Sections were washed in PBS before incubation with secondary antibodies or labelled streptavidin (Supplementary Table 4) for 1hr at room temperature followed by mounting with DAPI containing fluoromount (Southern Biotech). Small to medium sized airways in lung sections were captured by an investigator blind to sample identity with an EVOS FL imaging system (Thermo) ...
-
bioRxiv - Microbiology 2023Quote: ... 4°C) and incubated with goat anti-human IgG-Alexa Fluor 647 and goat anti-human IgM-PE (both Southern Biotech) for 30 minutes at 4°C at 600 rpm ...