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Citations for SouthernBiotech :
101 - 150 of 535 citations for 6 1 BROMO ETHYL 4 CHLORO 5 FLUOROPYRIMIDINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... coated with 100 µL/well of goat anti-mouse IgA (5 µg/mL Bic 0.1M, pH 9.6; Southern Biotech) were incubated with serial 3-fold dilutions of either fecal supernatant (100 µL ...
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bioRxiv - Microbiology 2020Quote: ... followed by staining with goat F(ab’)2 anti-human kappa-Alexa Fluor 647 (5 μg/ml, Southern Biotech) (Supporting figure 1A).
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bioRxiv - Microbiology 2023Quote: ... followed by staining with goat F(ab’)2 anti-human kappa-Alexa Fluor 647 (5 μg/mL, Southern Biotech), fixation in PBS 1% formaldehyde and analysis by flow cytometry (BD FACSVerse).
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bioRxiv - Molecular Biology 2020Quote: ... and a 1:1 mix of anti-rabbit (Southern Biotech, 4050– 05) and anti-rabbit (Cell Signaling ...
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bioRxiv - Molecular Biology 2022Quote: ... The following antibodies were used: collagen 1 (1:1000, Southern Biotech, 1310), collagen 3 (1:500 ...
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bioRxiv - Immunology 2022Quote: ... were coated overnight at 4°C with 2 μg/mL of monkey cross-adsorbed goat anti-human IgG (Southern Biotech #2049-01) diluted in phosphate-buffered saline (PBS) ...
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bioRxiv - Developmental Biology 2021Quote: ... The sections were then washed three times for 5 min with PBST and slides were mounted with Fluoromount-G containing DAPI and photobleaching inhibitors (SouthernBiotech). All steps were performed in a dark humid chamber to prevent evaporation and photobleaching ...
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bioRxiv - Cell Biology 2021Quote: ... coverslips were washed in PEM supplemented with 0.01% Triton-X-100 for 5 minutes before mounting on clean slides using DAPI Fluoromount G (Southern Biotech) or Prolong Gold.
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bioRxiv - Immunology 2021Quote: ... at 1×106 cells/well and stimulated for 48 hours with 5 μg/mL of RBD-His or hCD81-His or media alone (RPMI + 5% FCS) in the presence of anti-mouse CD28 antibody [0,5 μg/ml] (Southern Biotech). As positive control cells were stimulated with anti-mouse CD3 [0,05 μg/ml] (Southern Biotech) ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 5 times with PBS-T and incubated with goat anti-mouse IgG1 antibody conjugated with horseradish peroxidase (SouthernBiotech) for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... slides were washed 2 x for 5 minutes in PBS-tx and 3 x for 5 minutes in PBS and cover slipped with DAPI Fluoromount-G (SouthernBiotech).
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed three times in 1X PBS for 5 min before being mounted on slides with Fluoromount-G (SouthernBiotech).
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed with PBS-T for 5 mins at room temperature and then mounted with DAPI-Fluoromount-G (SouthernBiotech) to adhere coverslips ...
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bioRxiv - Genetics 2021Quote: ... Primary antibodies (1:250 Anti-Fibronectin-1, F3648, Sigma-Aldrich; 1:100 Goat Anti-Type I Collagen, 1310-01, Southern Biotech; 1:100 Goat Anti-Type V Collagen ...
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bioRxiv - Bioengineering 2023Quote: ... Sections were washed with 0.1% v/v Tween 20 in 1×PBS twice and 1×PBS before mounting glass coverslips with Fluoromount G (Southern Biotech). Images were acquired with a Nikon A1 RMP confocal using a 10× objective and analysed using ImageJ.
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bioRxiv - Immunology 2021Quote: ... IgM or IgA were detected by adding HRP-conjugated goat anti-mouse IgG (4 μg/ml; Southern Biotech, Cat#1030-05; RRID: AB_2619742), goat anti-mouse IgM (4 μg/ml ...
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bioRxiv - Immunology 2019Quote: ... Sera were incubated 2 h at 37°C on wells pre-coated with 5 μg/mL of NP15-BSA or goat-anti-mouse IgM (Southern Biotech) and saturated with PBS 2% BSA ...
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bioRxiv - Immunology 2019Quote: ... culture supernatants were incubated 2 h at 37°C on wells pre-coated with 5 μg/mL of goat-anti-mouse IgM (Southern Biotech) and saturated with PBS 2% BSA ...
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in calcium-containing HBSS and incubated at room temperature for 5 minutes before activation by anti-IgM F(ab′)2 (Southern Biotech) or VLPs ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were then rinsed again three to four times in PBS for 5-10 min and mounted on Fisherbrand microscope slides and cover slipped using Fluoromount-G (Southern Biotech) mounting medium.
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bioRxiv - Neuroscience 2020Quote: ... All coverslips were then washed three times (five minutes per wash) in 1xPBS followed by block and permeabilization with 5% (v/v) normal goat serum (NGS, Southern Biotech) and 0.25% (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were subsequently washed three times for 5 min each in PBS and mounted on microscope slides (Brain Research Laboratories) using Fluoromount-G mounting medium (Southern Biotech). Images were obtained with an EVO FL Imaging System equipped with 4x objective to visualize entire ACA with the adjacent cortical areas simultaneously ...
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bioRxiv - Physiology 2020Quote: ... Sections were again washed 3 times 5 minutes with 1x PBS followed by mounting with coverslips using Fluoromount-G mounting media containing DAPI nuclear stain (Southern Biotech).
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bioRxiv - Immunology 2022Quote: ... NP-OVA specific IgG1 was detected as previously described48 using plates were coated with TNP(5)-BSA and detected using goat anti-mouse IgG1-HRP (Southern Biotech).
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bioRxiv - Genomics 2022Quote: ... and incubated overnight with 1) goat anti-collagen I (1:200; SouthernBiotech; #1310-01) and rabbit anti-MFAP4 (1:100 ...
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bioRxiv - Immunology 2024Quote: ... IgG1 (1:5000, Southern Biotech), IgG2c (1:5000 ...
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bioRxiv - Immunology 2024Quote: ... IgG2c (1:5000, Southern Biotech) Ab for 1 hour at 30C ...
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bioRxiv - Microbiology 2023Quote: ... Pellet was then resuspended in 100 µL ice-cold PBS containing 4 µg of FITC-coupled goat anti-mouse IgA antibody (SouthernBiotech, Imtec Diagnostic, #1040-02). The ratio of 4 µg of the respective antibody to stain 109 bacteria was previously evaluated to be the maximum amount of antibody that can be used without resulting in unspecific staining of non-IgA coated bacteria by using fecal samples from Rag1−/− mice as non IgA-coated negative controls ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips with stained cells were washed 2 x 5 min in PBS and then mounted with Fluoromount-G (Southern Biotech 0100-01) for image acquisition ...
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bioRxiv - Cancer Biology 2020Quote: ... All cells were maintained at 37 ◻C with 5% CO2 and were mycoplasma free as determined a PCR assay (Southern Biotech).
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bioRxiv - Microbiology 2023Quote: ... cells were attached to a poly-L-lysine treated slide before 5 μl of Fluoromount G with DAPI (Cambridge Bioscience, Southern Biotech) was added and coverslip applied ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... at the level of injection (L3-5) as well as slices from corresponding DRGs (20 µm thickness) were directly mounted with DAPI Fluoromount-G® (SouthernBiotech™) and imaged using Axio Scan Z1 ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed with PBS (3 x 5 minutes) and mounted on standard microscope glass slides using DAPI-Fluoromount G (SouthernBiotech, 0100-20). Antibodies and dilutions used were as follows ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were then washed with PBS-BSA and stained with secondary antibodies (5 μM Alexa Fluor® 488 anti-mouse (SouthernBiotech; 1031-30) and 10 μM Alexa Fluor® 647 anti-human (SouthernBiotech ...
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bioRxiv - Neuroscience 2021Quote: ... sections were washed three times with PBS containing 0.3% Tween-20 (PBST) for 10 min and treated with 5% normal donkey serum (NDS; Southern Biotech, cat#0030-01) in PBST for 1 h at room temperature for blocking ...
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bioRxiv - Neuroscience 2022Quote: ... A final wash of 3 × 5 minutes was performed prior to mounting using DAPI Fluoromount-G® (Southern Biotech, Cat No. 0100-20). Slices were imaged on a Zeiss LSM 800 laser confocal scan head mounted on a Zeiss Z1Axio Observer inverted microsope frame (Carl Zeiss ...
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bioRxiv - Biochemistry 2020Quote: ... the plate was washed extensively (5×) with PBS-T and incubated with a HRP-conjugated mouse anti-human IgG (9040-05, clone JDC-10, Southern Biotech, Birmingham, AL) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were then washed a final time with PBST 5 times for 10 min per wash and then mounted with Fluoromount-G (SouthernBiotech, catlog # OB100-01).
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bioRxiv - Neuroscience 2023Quote: ... Cells were then fixed in 4% paraformaldehyde in Hank’s balanced salt solution (HBSS) for 30 min at room temperature and washed 5 times in HBSS before mounting using Fluoromount with DAPI (Southern Biotech, Birmingham, AL).
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bioRxiv - Immunology 2023Quote: ... primary B cells (107/ml) were stained for 5 min on ice with 10 μg/ml of biotinylated anti-IgM (Southern Biotech, 1021-08) and washed with Imaging Buffer ...
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bioRxiv - Immunology 2023Quote: ... sonicated to disrupt bead:bead aggregates (Bioruptor®: HIGH, 5 cycles, 30” on/off) and coated with different ratios of biotinylated anti-IgM (Southern Biotech, #1021-08) and biotinylated Eα52-68 peptide (Biotin-GSGFAKFASFEAQGALANIAVDKA-COOH ...
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bioRxiv - Neuroscience 2023Quote: ... Tissues were washed three more times for 5 minutes each in PBST and then mounted using DAPI Fluoromount-G (0100-20, Southern Biotech, Birmingham, AL). Slides were imaged using a 20x objective on a Zeiss laser scanning confocal microscope (LSM710 ...
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bioRxiv - Microbiology 2023Quote: ... Coverslips were then incubated in secondary antibody in blocking buffer for 1 h at RT followed by 3×5 min washes in PBS and then mounted in Fluoromount G with DAPI (Southern Biotech, Birmingham, AL).
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bioRxiv - Neuroscience 2024Quote: ... The specimens were subjected to a triple wash with PBS and treated with a 5% normal donkey serum (NDS; Southern Biotech, #0030-01) solution in PBST (PBS with 0.1% Triton X ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then fixed in 3.7% paraformaldehyde in Hank’s balanced salt solution (HBSS) for 30 min at room temperature and washed 5 times in HBSS before mounting using Fluoromount with DAPI (Southern Biotech, Birmingham, AL). Images were acquired using a Zeiss LSM 880 inverted confocal microscope (Carl Zeiss ...
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bioRxiv - Neuroscience 2023Quote: ... anti-sheep (Novus NBP1-73267) or a 1:1 mix of anti-rabbit (Southern Biotech; OB405005) and anti-rabbit (Cell Signaling ...
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bioRxiv - Neuroscience 2021Quote: ... Collagen IV (SouthernBiotech, Goat 1:100), Thbs1 (Invitrogen ...
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bioRxiv - Immunology 2021Quote: To detect mouse Ig Nunc MaxiSorp ELISA plates were coated for 1h at room temperature with goat anti-mouse Ig (H+L) (4 μg/ml; Southern Biotech, Cat#1010-01; RRID: AB_2794121). Citrobacter rodentium-specific Ig was quantified by coating Nunc MaxiSorp ELISA plates with 108 bacteria/well heat-inactivated at 60°C for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... and 1/5,000 HRP-Steptavidin (100 μL/well) was added for 1 h (Southern Biotech, #7105-05). Plates were washed 4 times again with PBS-Tween 20 0.05 % ...
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bioRxiv - Cell Biology 2024Quote: ... or HRP-conjugated α-mouse IgM (Southern Biotech, 1020-05, 1:5000 in 1 % BSA in PBS) for 4 h at room temperature ...