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Citations for SouthernBiotech :
401 - 450 of 499 citations for 1 Bromo 3 difluoromethoxy benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed 3x with DPBS and coverslips mounted on 1 mm glass slides using Fluoromount-G (SouthernBiotech, #0100-01). Cells were imaged using a Zeiss Axio Observer 7 fitted with a 40x objective using DAPI and Alexa Fluor 488 filters ...
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bioRxiv - Biochemistry 2024Quote: ... PVDF membranes containing blotted proteins were washed in excess PBS-Tween 20 for 1h at room temperature before application of a 1:2000 dilution of primary mouse Anti-Human IgA1 antibodies conjugated to biotin (SouthernBiotech). Blots were washed 10 min 3 x in PBS followed by treatment with a 1:2000 dilution of ExtrAvidin®−Peroxidase conjugate (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... Coverslips were washed in 1× PBS and mounted on glass slides with Fluoromount G (Southern Biotech, with or without DAPI).
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bioRxiv - Immunology 2024Quote: ... Membranes were incubated in TBST containing 5% (w/v) skimmed milk with goat anti-mouse IgG conjugated to horseradish peroxidase (HRP) (1:2000, 1010-05, SouthernBiotech) for 3h at 4°C in a rotary platform and washed thrice with TBST for 10 min each time ...
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bioRxiv - Immunology 2024Quote: ... wells were washed six times with PBS supplemented with 0.1 % Tween20 and incubated for 2 h with 1 μg/mL biotinylated goat α-mouse IgM or IgG1 (SouthernBiotech) at 37 °C ...
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bioRxiv - Bioengineering 2023Quote: ... The staining procedure involved the following steps: (1) The sections were blocked in a solution of 10% normal goat serum (SouthernBiotech) and 0.3% Triton-X in PBS for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... 0,1 % BSA and washed three times with 1× PBS before mounting on microscope slides using Fluoromount-G (Cat# 0100-01, SouthernBiotech,). Hoechst 33342 dye (Cat# B2261 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 × 105 cells in 100 μL per well were plated in a 96-well plate pre-coated with anti-mouse CD3ε (1 μg/mL, SouthernBiotech). Tumors were harvested from mice 10 days post inoculation and dissociated using gentleMACS (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... A volume of 25 μL of a 1:1,000 dilution of Mouse Anti-Biotin-AP (Southern Biotech, cat. #6404-04) in blocking buffer was added to each of the wells of the HN plates and incubated for 1 hr at RT ...
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bioRxiv - Immunology 2024Quote: ... 25 μl of undiluted supernatant was added to wells coated with 1 μg/ml goat anti-mouse Ig (Southern Biotech) or HAPR8 and for serum samples 3-fold serial dilutions of serum samples starting at 1/100 were added to wells coated with NP4-BSA (10 μg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... the tissue was allowed to dry on the slides at 4 °C and then briefly re-wet with 1 M PBS before being covered with DAPI Fluoromount-G (SouthernBiotech) and coverslipped ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then stained with iamidino-2-phenylindole (DAPI; 1:10000) to label nuclei and mounted in Fluoromount-G Mounting Medium (SouthernBiotech). Images of whole sections were acquired with a Zeiss Axio ScanZ1 slide scanner using a 20x objective lens and Zen Blue 3.1 software (Carl Zeiss ...
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bioRxiv - Immunology 2024Quote: ... PE-labeled detector antibody (mouse anti- human IgG-Fc; Southern Biotech, Cat#9040–09, clone JDC-10; 1 μg/ml) was added and beads were incubated at room temperature for 1h on a shaker ...
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bioRxiv - Microbiology 2020Quote: ... IgG-bound DNP-beads were next incubated with 1 µg/mL Alexa Fluor647-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech) in PBS-TH.
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bioRxiv - Biochemistry 2021Quote: ... A baseline of 30 s was recorded before stimulation with 1 µg/ml anti-λ antibody (Southern Biotech, Birmingham, AL, USA). Flow cytometry standard files were analyzed using FlowjoTM software for Windows ...
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bioRxiv - Immunology 2021Quote: ... were coated with 2.5 µg/ml of mouse anti-human c-Myc 9E10 mAb (produced in house) or goat anti-human IgG (1:2000, Southern Biotech) for the standard ...
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bioRxiv - Immunology 2021Quote: ... This will be followed by a 1 h incubation with biotinylated HRP conjugated goat anti-mouse IgG subtype antibodies (Southern Biotech). 3,3’,5,5’ tetramethylbenzidine (TMB ...
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bioRxiv - Biochemistry 2020Quote: ... and then incubation for 30 mins at room temperature with anti-human IgG HRP at 1:4000 (Southern Biotech #2040-05). 5 washes of TBST were done followed by development using 1-Step Ultra TMB-ELISA Substrate Solution (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were washed in 1 x PBS three times and mounted and then cover-slipped with Fluoromount-G with DAPI (Southern Biotech).
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bioRxiv - Neuroscience 2020Quote: ... slides were incubated at room temperature for 2 hours in secondary antibodies diluted in the blocking solution (at 1:500 for Alexa-coupled antibodies (Life Sciences)) and then mounted using Hoechst-containing Fluoromount-G (Southern Biotech).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... before adding 50 μL/well of a 1:5000 dilution of HRP-conjugated goat anti-mouse IgG (Southern Biotech, #1030-05) in TBSF+MnCl2 ...
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bioRxiv - Microbiology 2022Quote: ... the beads were incubated with 1 µg/mL Alexa Fluor647-conjugated goat F(ab’)₂ anti-human kappa (Southern Biotech, 2062-31) in PBS-TH for 30 min at 4 °C with shaking ...
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bioRxiv - Immunology 2022Quote: ... a standard curve for mouse isotype was established for each plate by coating 2 columns with 50 μL goat anti-mouse Ig (1 μg/mL in PBS; Southern Biotech) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... 3 columns on each plate were coated with 1:1000 dilution each of goat anti-mouse Kappa and Lambda light chains (Southern Biotech). After overnight incubation at 4ºC ...
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bioRxiv - Microbiology 2020Quote: ... followed by the addition of horseradish peroxidase-conjugated goat anti-mouse-IgG Fc secondary antibody (1:2000) (Southern Biotech, 1033-05). After incubation for 2 hours at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... in PBST or PBSTF or 1:1000 anti-hamster-IgG2/IgG3-HRP in PBST or PBSTF (Southern Biotech Cat. #1935-05). Plates were incubated at room temperature for 1-2 h ...
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bioRxiv - Immunology 2022Quote: ... The plates were then washed with PBS containing 0.1% Tween-20 (PBS-T), and Alkaline Phosphatase-conjugated Goat Anti-Human IgG (dilution 1:500, catalog no. 2040-04) from Southern Biotech was added and incubated for 45 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Antibody binding to the spike proteins was determined using a 1:2000 diluted HRP conjugated goat anti-human IgG (ITK Southern Biotech) for 1 h at RT and tetramethylbenzidine substrate (BioFX) ...
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bioRxiv - Microbiology 2020Quote: ... the peptide arrays were incubated with a 1/1000 dilution of an appropriate antibody peroxidase conjugate (goat anti-human HRP conjugate, Southern Biotech, cat ...
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bioRxiv - Microbiology 2021Quote: ... Fab fragments were detected with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech, 1:500). After washing ...
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bioRxiv - Immunology 2021Quote: ... membranes were incubated with secondary antibodies conjugated to alkaline phosphatase anti-IgM or anti-IgG diluted 1:2000 (rabbit anti-mouse IgM, Jackson ImmunoResearch, goat anti-mouse IgG, SouthernBiotech). Substrate NBT/BCIP (Promega ...
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bioRxiv - Immunology 2021Quote: ... incubated for 1 hour at room temperature with mouse anti-human IgG conjugated to horse radish peroxidase (Southern Biotech, Birmingham, USA) diluted 1:5000 in blocking solution ...
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bioRxiv - Microbiology 2023Quote: ... Alexa Fluor® Plus 488 secondary antibodies were diluted 1:1,000 in the respective blocking solutions before mounting with 5 µL DAPI Fluoromount-G® (Southern Biotech) and imaging ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed in PBS and incubated in secondary antibody solution for 1 hour before being mounted on Superfrost Plus slides and coverslipped with DAPI-containing Fluoromount-G (0100-20, Southern Biotech).
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bioRxiv - Microbiology 2023Quote: ... anti-IgG2 Fc-Alexa Fluor 488 and mouse anti-IgG3 hinge-Alexa Fluor 647 (1 µg/ml, all from Southern biotech) for an additional 20 minutes at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Precomplexation of the anti-sialyl-LewisX antibody was performed with a 1:50 dilution of goat anti-mouse IgM-HRP (#1021-05, Southern Biotech) and 0.65 µg donkey anti-goat Alexa Fluor 555 (A21432 ...
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bioRxiv - Molecular Biology 2023Quote: ... and primary antibodies were added in 1 : 10000 dilution (anti-IgG Fc cat. 31789, Thermo Fisher; anti-His-Tag cat. 4603-08, SouthernBiotech) followed by 1 h incubation at RT ...
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bioRxiv - Neuroscience 2023Quote: ... diluted 1:1000 in PBST in order to detect the primary antibody for 1 h at room temperature before being mounted on glass slides and coverslipped with Fluoromount-G (0100-01, Southern Biotech). Images of cresyl violet stained sections were obtained using an Axio Observer Carl Zeiss light microscope whereas fluorescent images from immunolabelled sections were collected using an Axio Observer Carl Zeiss fluorescent microscope ...
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bioRxiv - Microbiology 2024Quote: ... Following additional washings and incubation at 37°C for 1 h with AP-conjugated anti-goat IgG secondary antibody (Southern Biotech), absorbance was read by addition of PNPP ...
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bioRxiv - Immunology 2022Quote: ... at 37 °C for 1 hr followed by the multi-species SP ads-HRP-conjugated goat anti-mouse IgG (H+L) antiserum (Southern Biotech) diluted at 1:20.000 and incubated at 37 °C for 1 hr ...
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bioRxiv - Physiology 2023Quote: ... Non-specific antibody binding was blocked for 1 hour at room temperature with a blocking solution containing: 5% normal goat serum (006001, SouthernBiotech™), 0.3% Triton™ X-100 (X100 ...
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bioRxiv - Biochemistry 2023Quote: ... then washed and secondary antibody added at 1:2000 or 1:4000 dilution in blocking buffer (100□μl/well) using either anti-mouse IgG-HRP (Catalog #1030-05, Southern Biotech), or anti-mouse IgA-biotin (Catalog #1040-80 ...
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bioRxiv - Cell Biology 2023Quote: ... Labeling of primary antibodies was visualized with anti-mouse PE and anti-rat PE (both Southern Biotech, USA, 1:1000 diluted). Actin in living cells was visualized with SiR actin (Spirochrome ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies conjugated with HRP used in the study are: Goat Anti-mouse IgG1-HRP (1:10000, Southern Biotech, 1071-05), Goat Anti-rabbit IgG-HRP (1:10000 ...
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bioRxiv - Immunology 2024Quote: ... diluted to 1 μg/ml in T-PBS followed by incubation for 1 hour with goat-anti mouse IgG conjugated with HRP (Southern Biotech) diluted 2000 × in T-PBS.
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bioRxiv - Immunology 2024Quote: ... diluted to 1 μg/ml in T-PBS followed by incubation for 1 hour with goat-anti mouse IgG conjugated with HRP (Southern Biotech) diluted 2000 × in T-PBS ...
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bioRxiv - Immunology 2024Quote: ... Specific IgG was detected for 1 hour at room temperature with biotin rat anti-mouse IgG (Southern Biotech, Birmingham, AL, USA) biotin rat anti-mouse IgG1 (Southern Biotech) ...
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bioRxiv - Immunology 2024Quote: ... samples were incubated with 100 μl of a 1:500 diluted phycoerythrin (PE)-tagged goat-anti mouse IgG-Fc (Southern Biotech) in the dark at RT with shaking at 600 rpm for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... Wells then were incubated for 1 hour with horseradish peroxidase (HRP)-conjugated goat anti-human IgG (monkey pre-adsorbed) (Southern Biotech) at a 1:4,000 dilution ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed with ELISA wash buffer then incubated for 1 h at RT with an HRP conjugated goat anti-mouse (Southern Biotech). Plates were washed with ELISA wash buffer and developed using 1-Step Ultra TMB-ELISA substrate solution (Thermo Fisher) ...