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Citations for SouthernBiotech :
351 - 400 of 1212 citations for Mouse Anti Human IgG Fab Alexa Fluor 594 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The following secondary antibodies were used: rabbit-anti mouse IgG-horseradish peroxidase (HRP; SouthernBiotech; dilution 1:10‘000) and goat-anti rabbit IgG-HRP (SouthernBiotech ...
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bioRxiv - Biochemistry 2020Quote: ... the culture media were tested for their ACE2-Fc contents using a custom ELISA (anti-ACE2 antibody [Abcam, Cambridge, MA] for capturing and anti-human IgG-Fc-HRP [SouthernBiotech, Birmingham, AL] for detection). Clones with the highest expression of recombinant ACE2-Fc variants were selected ...
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bioRxiv - Cell Biology 2020Quote: ... and probed using the indicated primary antibodies (Table SX) and anti-mouse or anti-rabbit IgG-linked HRP secondary antibodies (Southern Biotech) followed by ECL (Luminata Crescendo or Forte ...
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bioRxiv - Immunology 2024Quote: ... membranes were incubated with primary antibodies overnight at 4 °C and during 2 hours at room temperature with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit IgG (SouthernBiotech, USA). Membranes were then revealed using G:BOX Chemi XL1.4 Fluorescent & Chemiluminescent Imaging System (Syngene ...
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bioRxiv - Microbiology 2023Quote: ... formaldehyde fixed parasites were adhered to glass slides and blocked with 50% FCS in PBS for 45 min before staining with primary anti-VSG (1:10,000) and secondary Alexa Fluor (1:1,000) antibodies and mounting with DAPI Fluoromount-G® (Southern Biotech). Imaging was performed with an Axioscope 2 fluorescence microscope (Zeiss ...
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bioRxiv - Microbiology 2023Quote: ... formaldehyde fixed parasites were adhered to glass slides and blocked with 50% FCS in PBS for 45 min before staining with primary anti-VSG (1:10,000) and secondary Alexa Fluor (1:1,000) antibodies and mounting with DAPI Fluoromount-G® (Southern Biotech). Imaging was performed with an Axioscope 2 widefield fluorescence microscope (Zeiss ...
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bioRxiv - Immunology 2022Quote: ... Plates were then washed with TBST and incubated with secondary HRP-conjugated goat anti-human IgG (Southern Biotech, Birmingham, AL) at a 1:15,000 dilution for 45 minutes at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by detection with a 1:10,000 dilution of goat anti-human IgG-HRP (Southern Biotech 2040-05, Birmingham, AL). Plates were developed for 5 minutes with TMB substrate (VWR 95059-286) ...
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bioRxiv - Immunology 2020Quote: ... MagPlex beads were washed with PBST (PBS, 0.05% Tween-20) and incubated with anti-human IgG Fc-PE (Southern Biotech). Finally ...
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bioRxiv - Immunology 2022Quote: ... plates were washed with TBS containing 0.05% Tween-20 (TBST) and resuspended in 50 μL of Goat-anti-human IgG-PE (Southern Biotech). After 2 hours of incubation on a rotator at room temperature ...
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bioRxiv - Immunology 2019Quote: ... Anti-IgM or anti-IgG-HRP (Southern Biotech) were added (1:2000 ...
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bioRxiv - Immunology 2023Quote: ... then incubated with an HRP-conjugated human/bovine/horse cross-adsorbed anti-mouse secondary antibody (Southern Biotech) at a 1:20,000 dilution for 1 hour at RT ...
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bioRxiv - Immunology 2023Quote: ... beads were incubated with anti-IgG::PE or anti-IgG::AF488 (2.5 µg/mL, Southern Biotech) in PBS with 1% BSA for 15 min ...
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bioRxiv - Immunology 2020Quote: ... Plates were then incubated with a 1:1000 dilution of mouse serum raised against ZE3-6H followed by goat anti-mouse IgG HRP conjugate (Southern Biotech, Birmingham, AL, USA) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies used include the following: Goat Anti-Mouse IgG (H+L; Southern Biotech, 1031-05, 1:2,000) and Goat Anti-Rabbit IgG (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... incubated with a horseradish peroxidase-conjugated goat anti-mouse IgG secondary antibody (SouthernBiotech, 1030-05, 1:2000 dilution) at RT for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... followed by addition of 50 µL of 1:1,000 anti-mouse IgG-HRP (Southern Biotech Cat. #1030-05) in PBST or 1:1000 of anti-mouse IgA-HRP in PBSTBA (SouthernBiotech) ...
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bioRxiv - Immunology 2021Quote: ... HRP-conjugated mouse anti-histidine-tag IgG (1:4000) (catalog number 4603-05, Southern Biotech, Birmingham, AL, USA) was added for 1 hour followed by addition of TMB substrate ...
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bioRxiv - Immunology 2020Quote: ... plates were incubated with anti-mouse IgG secondary Ab (Southern Biotech, cat no 1170-05, dilution 1:1000) 1hr at RT ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed three times followed by addition of goat anti-mouse IgG horseradish peroxidase conjugated (Southern Biotech) diluted in blocking solution (1:5000) ...
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bioRxiv - Microbiology 2020Quote: ... followed by addition of 50 µL of 1:2000 anti-mouse IgG-HRP (Southern Biotech Cat. #1030-05) in PBST or 1:10000 of biotinylated anti-mouse IgG ...
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bioRxiv - Immunology 2019Quote: ... we pre-coated 96-well multi-screen plates with 1 μg/ml goat anti-mouse IgG (Southern Biotech) or 20 μg/ml poly-L-lysine (Sigma ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed three times followed by addition of goat anti-mouse horseradish peroxidase-conjugated IgG (Southern Biotech) diluted in blocking solution (1:5000) ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed 3x with PBS and treated with an AP conjugated anti-mouse IgG antibody (Southern Biotech) for 1.5 hours ...
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bioRxiv - Microbiology 2023Quote: ... followed by addition of 50 μL of 1:2000 anti-mouse IgG-HRP (Southern Biotech, Cat. #1030-05), or anti-mouse IgA- biotin (Southern Biotech ...
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bioRxiv - Immunology 2023Quote: ... and incubated for 1 hour with mouse anti-monkey IgG HRP-conjugated secondary antibody (Southern Biotech, clone SB108a) at a 1:5,000 dilution or anti-monkey IgM HRP-conjugated secondary antibody (Rockland ...
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bioRxiv - Immunology 2023Quote: ... the plate was incubated with HRP-conjugated goat anti-mouse IgG (1:4000) (Southern Biotech, Birmingham, AL, USA) and color reaction was developed by adding TMB (Invitrogen™ ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed three times followed by addition of goat anti-mouse IgG horseradish peroxidase conjugated (Southern Biotech) diluted in blocking solution (1:5000) ...
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bioRxiv - Microbiology 2024Quote: ... followed by addition of 50 μL of 1:2000 anti-mouse IgG-HRP (Southern Biotech Cat. #1030-05) in PBST ...
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bioRxiv - Microbiology 2024Quote: ... and incubated for 1 h with mouse anti-rhesus IgG HRP-conjugated secondary antibody (Southern Biotech, clone SB108a) at a 1:5,000 dilution or anti-rhesus IgM HRP-conjugated secondary antibody (Rockland ...
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bioRxiv - Immunology 2023Quote: ... and incubated for 1 hour at room temperature with horseradish peroxidase–conjugated goat anti–mouse IgG (Southern Biotech) diluted 1:4000 with assay diluent ...
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bioRxiv - Biochemistry 2020Quote: ... and then incubation for 30 mins at room temperature with anti-human IgG HRP at 1:4000 (Southern Biotech #2040-05). 5 washes of TBST were done followed by development using 1-Step Ultra TMB-ELISA Substrate Solution (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed with water three times before applying 25 μl of secondary antibody (goat anti-human IgG Fc-Southern Biotech, 2048-04 ...
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bioRxiv - Immunology 2022Quote: ... IgG bound to the rSARS-CoV-2 S protein was detected using a horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody (Southern Biotech) incubated for 1 hour ± 10 minutes at 24°C ± 2°C ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed three times and incubated with the goat anti-human-IgG Fc secondary antibody conjugated with alkaline phosphatase (AP, Southern Biotech) at a 1:1000 dilution in blocking buffer for 1 h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... The plates were then washed with PBS containing 0.1% Tween-20 (PBS-T), and Alkaline Phosphatase-conjugated Goat Anti-Human IgG (dilution 1:500, catalog no. 2040-04) from Southern Biotech was added and incubated for 45 min at room temperature ...
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bioRxiv - Immunology 2019Quote: ... the membrane was re-blocked with 5% BSA in 1x TBST for 2 hours at room temperature before probing with 1:5000 dilution of anti-human IgG-HRP (Southern Biotech) in 5% BSA/1x TBST for 1 hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Antibody binding to the spike proteins was determined using a 1:2000 diluted HRP conjugated goat anti-human IgG (ITK Southern Biotech) for 1 h at RT and tetramethylbenzidine substrate (BioFX) ...
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bioRxiv - Immunology 2022Quote: ... The cells were then stained with 50 μL of the secondary mix (goat anti-human IgG-AF488 (SouthernBiotech, cat. #2040-30) and goat anti-mouse Alexa Fluor 488 IgG (H + L ...
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bioRxiv - Immunology 2022Quote: ... The microtiter plates were developed with horseradish peroxidase (HRP)-labeled goat anti-human IgG or IgM polyclonal antibodies (SouthernBiotech, Birmingham, AL). The plates were developed with 3,3 ...
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bioRxiv - Immunology 2022Quote: ... The plates were then washed three times with water and 200 μl of goat anti-human IgG HRP secondary antibody (Southern Biotech) diluted to a ratio of 1:2000 in block buffer was added and incubated for 1 hr at room temperature followed by an hour of incubation ...
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bioRxiv - Cell Biology 2023Quote: ... Surface-bound sLAP was detected with anti-human PE antibodies that bound directly to the Fc-IgG part of the chimeric sLAP proteins (SouthernBiotech, USA). PE signals were analyzed with FlowJo software (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and stained with AF647-conjugated goat anti-human IgG or IgA as appropriate (2050-31 and 2040-31, Southern Biotech) and analyzed by flow cytometry as described above.
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bioRxiv - Immunology 2023Quote: ... Beads were then washed with PBST and reacted for 30 minutes at room temperature with biotinylated goat anti-human IgG (Southern Biotech) or goat whole IgG containing anti-monkey IgA (Novus ...
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bioRxiv - Immunology 2021Quote: ... IgG antibodies were detected with peroxidase-labeled anti-monkey IgG (Southern Biotech), followed by tetramethylbenzidine (TMB ...
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bioRxiv - Cancer Biology 2023Quote: ... was coated with hPD-L1-His protein and anti-PD-L1 antibody and anti-mouse IgG specific HRP conjugated secondary antibodies (SouthernBiotech, Birmingham, AL, USA) were added ...
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bioRxiv - Immunology 2023Quote: ... and incubated with 0.75x serial dilutions of purified polyclonal human antibodies (IgG, SouthernBiotech, Cat # 0150-01 ...
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bioRxiv - Microbiology 2020Quote: ... IgG-bound DNP-beads were next incubated with 1 µg/mL Alexa Fluor647-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech) in PBS-TH.
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bioRxiv - Microbiology 2022Quote: ... the beads were incubated with 1 µg/mL Alexa Fluor647-conjugated goat F(ab’)₂ anti-human kappa (Southern Biotech, 2062-31) in PBS-TH for 30 min at 4 °C with shaking ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed three times with PBS-T and secondary goat anti-mouse IgG-HRP antibody (Southern Biotech), diluted 1:4000 in ELISA buffer ...