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Citations for SouthernBiotech :
351 - 400 of 1266 citations for Anti human IgE Conjugated to 40 nm Gold since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... cells were incubated with goat anti-human IgG Fc-HRP (Southern Biotech, 2014-05) diluted at 1:1000 in Perm Wash for 1.5 h at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Goat F(ab’)2 anti-human IgM was from Southern Biotech (Birmingham, AL, USA). BD Phosflow Fix Buffer I and Perm Buffer III were from BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... Secondary detection antibody solutions of Mouse Anti-Human IgG Fc-HRP (SouthernBiotech, Birmingham, AL) diluted 1:10,000 in 1% milk PBST and Mouse Anti-Human IgA1-HRP/Mouse Anti-Human IgA2-HRP (SouthernBiotech ...
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bioRxiv - Microbiology 2021Quote: ... anti-human IgA1 Alexa Fluor 647 (clone: B3506B4, Southern Biotech Cat. No. 9130-31), anti-human IgA2 Alexa Fluor 488 (clone ...
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bioRxiv - Microbiology 2021Quote: ... anti-human IgA2 Alexa Fluor 488 (clone: A9604D2, Southern Biotech Cat. No. 9140-30). The samples were incubated for 30 minutes at 4 ° C and directly topped up with 1 mL of a 5 μM Hoechst 33342 solution (Thermo Fischer Scientific Cat ...
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bioRxiv - Microbiology 2020Quote: ... followed by goat anti-human kappa-Alexa Fluor 647 (5 μg/ml, Southern biotech) for another 20 minutes at 4°C to allow discrimination between cell adherent (FITC+/Alexa fluor 647+ ...
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bioRxiv - Immunology 2021Quote: Goat anti-human kappa cross-absorbed against mouse IgG (Southern Biotech catalog # 2061-01) and goat anti-human lambda cross-absorbed against mouse IgG (Southern Biotech catalog # 2071-01 ...
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bioRxiv - Microbiology 2023Quote: ... or goat F(ab’)2 anti-human IgM-PE (5 µg/ml, Southern Biotech) for 30 minutes at 4°C ...
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bioRxiv - Immunology 2023Quote: ... and 100 µL of anti-human IgG-Fc HRP (clone JDC-10, Southern Biotech), diluted 1:2000 in blocking buffer ...
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bioRxiv - Immunology 2024Quote: ... Western blots were probed with Goat Anti-Human lambda-UNLB (SouthernBiotech, ref 2070-01) or Goat Anti-Human kappa-UNLB (SouthernBiotech ...
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bioRxiv - Immunology 2024Quote: ... Slides were washed and stained with Alexa-488 labeled anti-human IgG (Southern Biotech) at rt using supplied buffers ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated at RT for 1 h with the following HRP-conjugated secondary antibodies: goat anti-rabbit IgG conjugated to HRP (Southern Biotech, 4010-05; 1:5000), and goat anti-mouse IgG conjugated to HRP (Southern Biotech ...
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bioRxiv - Immunology 2020Quote: ... The membrane was washed in PBS containing Tween-20 (0.05%) and was incubated for 1 h with horseradish peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (Southern Biotech) diluted 1:20,000 accordingly ...
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bioRxiv - Immunology 2023Quote: ... plates were washed in wash buffer (PBS-0.05%Tween 20) and incubated with AP-conjugated secondary antibodies (anti-IgM, anti-IgG1, Southern Biotech). Plates were washed and followed by adding alkaline phosphatase (AP) ...
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bioRxiv - Microbiology 2024Quote: ... plates were incubated with HRP-conjugated mouse anti-monkey IgG or goat anti-mouse IgG (Southern Biotech, Birmingham, AL, USA) for 1 h ...
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bioRxiv - Immunology 2022Quote: ... Anti-BMP6 and isotype control were injected at a dose of 10mg/kg body weight diluted in sterile low endotoxin PBS and injected intraperitoneally into mice.G-CSF complexes were prepared as described in (Rubinstein et al., 2013) using human G-CSF (Filgrastim) and anti-human G-CSF (Clone BVD11-37G10, Southern biotech, Cat. No. 10128-0). 7.5μg of anti-G-CSF and 1.5μg of G-CSF were complexed ...
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bioRxiv - Immunology 2021Quote: ... washed and detected with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (SouthernBiotech, Birmingham, AL). The ELISA plates were developed using SureBlue 3,3′,5,5′-tetramethylbenzidine (TMB ...
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bioRxiv - Immunology 2020Quote: ... at 1:20,000 dilution or HRP-conjugated polyclonal goat anti-chicken IgA (SouthernBiotech, Birmingham, AL) at 1:10,000 was used as a secondary antibody and plates were incubated for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by incubation with secondary antibody (anti-rat IgG HRP-conjugated, Southern Biotech, 1:10,000) for 1 h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Excess biotinylated anti-IgA antibody was removed by washing and streptavidin-conjugated HRP (Southern Biotech) was added ...
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bioRxiv - Immunology 2021Quote: ... Bound antibodies were detected using peroxidase-conjugated goat anti-mouse IgM heavy chain (Southern Biotech) or IgG (H+L ...
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bioRxiv - Systems Biology 2020Quote: ... HRP-conjugated antimouse-IgG (ICN Pharmaceuticals; 55564) or anti-rabbit-IgG (Southern Biotech; 4050-05) were used as secondary antibodies ...
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bioRxiv - Immunology 2021Quote: ... then incubation with HRP-conjugated goat anti-mouse IgG (catalog number 1030-05, Southern Biotech). Plates were then developed with 3,3’,5,5’-tetramethylbenzidine (TMB ...
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bioRxiv - Microbiology 2020Quote: ... HRP-conjugated goat anti-mouse IgG (Southern Biotech, cat no:1030-05, 1:5000 dilution), HRP-conjuated goat anti-horse IgG (Bellancom life H15034,1:2000 dilution ...
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bioRxiv - Immunology 2020Quote: ... Detection antibodies were subclass specific goat anti-mouse polyclonal R-PE-conjugated antibodies (Southern Biotech) used at a 1:200 dilution ...
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bioRxiv - Immunology 2020Quote: ... at 1:20,000 dilution or HRP-conjugated polyclonal goat anti-chicken IgA (SouthernBiotech, Birmingham, AL) at 1:10,000 was used as a secondary antibody ...
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bioRxiv - Immunology 2023Quote: ... One hundred μl horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (H+L) (Southern Biotech) at 1:4000 dilution in 0.1% BSA/TBST was added and incubated for 1 h ...
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bioRxiv - Immunology 2023Quote: ... then incubated with anti-rhesus IgG conjugated to HRP (Southern Biotech Cat. No.: 4700-05) diluted in blocking buffer for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... then incubated with anti-rhesus IgG conjugated to HRP (Southern Biotech Cat. No.: 4700-05) diluted in blocking buffer for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... then incubated with anti-rhesus IgG conjugated to HRP (Southern Biotech Cat. No.: 4700-05) diluted in blocking buffer for 1 hour ...
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bioRxiv - Immunology 2024Quote: ... followed by HRP-conjugated goat anti-rabbit secondary antibody (SouthernBiotech; 1:2,000 in Wash Buffer). Plates were washed and 100 μL TMB was added for 5-10 min followed by 100 µL Stop Solution ...
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bioRxiv - Biochemistry 2024Quote: ... and an HRP-conjugated rabbit anti-goat antibody (Southern Biotech, 6160-05, 1:5000 dilution). Membranes were washed three times for 5-minutes each using PBST between all the described steps.
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bioRxiv - Immunology 2021Quote: ... Plates were then washed before the adding goat anti-human IgG-HRP (SouthernBiotech, 2045-05) diluted 1:10,000 to all wells and incubating for 1 h at 37°C ...
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bioRxiv - Immunology 2021Quote: ... and goat anti-human lambda cross-absorbed against mouse IgG (Southern Biotech catalog # 2071-01) were coated onto 96-well Nunc Maxisorp flat-bottomed plates at 2 µg/mL in coating buffer (0.1 M sodium carbonate ...
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bioRxiv - Immunology 2023Quote: ... IgG2c-HRP (polyclonal) and anti-human IgG1-HRP were purchased from SouthernBiotech (Birmingham, AL, USA). Cytofix/Cytoperm buffer kit and Fc-blocking reagent were purchased from BD Biosciences (Franklin Lakes ...
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bioRxiv - Microbiology 2024Quote: ... ELISA plates were coated with 2 µg/ml of goat anti-human IgG (Southern Biotech) at 4°C overnight ...
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bioRxiv - Immunology 2022Quote: ... and binding was revealed using secondary goat anti-human IgG-AP (Southern Biotech, 2040-04). After washing ...
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bioRxiv - Immunology 2023Quote: ... and binding was revealed using secondary goat anti-human IgG-AP (Southern Biotech, 2040-04). After washing ...
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bioRxiv - Immunology 2024Quote: ... Secreted antibodies were detected using biotinylated goat anti-human IgG (Southern Biotech Cat# 2045-08), HRP Avidin D (Vector laboratories Cat# A-2004 ...
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bioRxiv - Immunology 2020Quote: ... 4 single cell clones of DT40 WT or Fam72a−/− were stained with anti-IgM-PE (1:40, clone M-1, SouthernBiotech) and 12 populations of 5×104 IgM− cells were sorted for each clone ...
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bioRxiv - Immunology 2021Quote: ... and double-distilled water to remove cells before being incubated with alkaline phosphatase-conjugated anti-mouse IgM or anti-mouse IgG1 (Southern Biotech) for 2 h at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Secondary antibodies used include goat anti-mouse IgG and goat anti rabbit-IgG both conjugated to horseradish peroxidase (HRP) (Southern Biotech). Forte Western HRP substrate (Millipore ...
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bioRxiv - Immunology 2021Quote: ... plates were washed with PBS-T and incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG1 or goat anti-mouse IgG2c (Southern Biotech) for 1 h ...
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bioRxiv - Immunology 2020Quote: ... Serum was subsequently added and antibodies were detected using goat anti-mouse IgG1 or goat anti-mouse IgG2c conjugated to HRP (Southern Biotech) and 1xTMB (Thermofisher ...
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bioRxiv - Immunology 2024Quote: ... membranes were incubated with primary antibodies overnight at 4 °C and during 2 hours at room temperature with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit IgG (SouthernBiotech, USA). Membranes were then revealed using G:BOX Chemi XL1.4 Fluorescent & Chemiluminescent Imaging System (Syngene ...
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bioRxiv - Immunology 2022Quote: ... After overnight incubation at 37°C and 5% CO2 plates were washed extensively and incubated with HRP-conjugated goat-anti-mouse IgA or goat-anti-mouse IgG (Southern Biotech). Subsequently ...
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bioRxiv - Immunology 2024Quote: ... The ELISPOT plate was washed five times with PBS and anti-mouse IgG1 or anti-mouse IgG2c antibodies conjugated with alkaline phosphatase (Southern Biotech) at 1:1000 in PBS were added and incubated at room temperature for 2 hours ...
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bioRxiv - Microbiology 2021Quote: ... The primary antibodies were detected by secondary antibody anti-mouse or anti-rabbit conjugated to horseradish peroxidase (Southern Biotech, 1:10,000 dilution) for 1 hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... plates were washed with PBS-T and HRP-conjugated goat anti-mouse IgG or anti-rat IgG (Southern Biotech, Birmingham, AL, USA) added for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... The next day slides were washed and incubated for 1 hr in a secondary biotin-conjugated anti-mouse antibody (1:200, goat anti mouse, SouthernBiotech, Birmingham, AL) mixed in blocking solution ...