Labshake search
Citations for SouthernBiotech :
301 - 350 of 439 citations for Human Protein Patched Homolog 2 PTCH2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... Plates were washed three times and incubated with horseradish peroxidase (HRP)-conjugated goat anti-human secondary antibody (ITK Southern Biotech) diluted 1:2000 in blocking buffer for 1 hour at RT ...
-
bioRxiv - Immunology 2022Quote: ... plates were washed with TBS containing 0.05% Tween-20 (TBST) and resuspended in 50 μL of Goat-anti-human IgG-PE (Southern Biotech). After 2 hours of incubation on a rotator at room temperature ...
-
bioRxiv - Immunology 2022Quote: ... IgG antibodies were detected using horseradish peroxidase (HRP)-conjugated goat anti-human IgG detection antibody (Southern Biotech, Birmingham, AL, USA) at a 1:4,000 dilution and colorimetric development was accomplished using 100 μL of 0.1% 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid ...
-
bioRxiv - Immunology 2023Quote: ... another microarray was developed with the second detection mix: goat anti-human kappa chain Alexa555 (Southern Biotech, Ref.:2060-32) and goat anti-human lambda chain Alexa647 (Southern Biotech ...
-
bioRxiv - Microbiology 2023Quote: ... was used except that plates were coated with anti-human kappa and lambda light chain specific mouse antibodies (Southern Biotech) at 1:1 ratio diluted to 1 μg/ml in PBS overnight ...
-
bioRxiv - Microbiology 2024Quote: ... Antibodies used for flow cytometry analysis performed in this study were: FITC-conjugated anti-human IgM (Southern Biotech #2020-02), goat anti-human IgG-UNLB (Southern Biotech #2040-01) ...
-
bioRxiv - Biochemistry 2024Quote: ... PVDF membranes containing blotted proteins were washed in excess PBS-Tween 20 for 1h at room temperature before application of a 1:2000 dilution of primary mouse Anti-Human IgA1 antibodies conjugated to biotin (SouthernBiotech). Blots were washed 10 min 3 x in PBS followed by treatment with a 1:2000 dilution of ExtrAvidin®−Peroxidase conjugate (Sigma ...
-
bioRxiv - Immunology 2021Quote: ... followed by goat F(ab’)2 anti-mouse IgG1-FITC (Southern Biotech). The percentage of living HUCCT1 cells was estimated by flow cytometry ...
-
bioRxiv - Immunology 2020Quote: ... Cells were stimulated with anti-mouse IgM-F(ab)2 (Southern Biotech), ionomycin (4μM ...
-
bioRxiv - Microbiology 2022Quote: ... or 2 μg/ml goat anti-hu-IgM-FITC (2020-02, SouthernBiotech) for 30 minutes at 4 °C while shaking ...
-
bioRxiv - Cell Biology 2023Quote: ... goat F(ab’)2 anti-mouse IgG-PE (Southern Biotech, Birmingham, AL); and anti-phospho-AKT (Ser473) ...
-
bioRxiv - Immunology 2021Quote: ... Plates were washed 4X in wash buffer and the secondary antibody Goat anti-Human Ig-HRP (Southern Biotech, Cat# 2010-05), was added and incubated at 37°C for 1 hr ...
-
bioRxiv - Biochemistry 2020Quote: ... and then incubation for 30 mins at room temperature with anti-human IgG HRP at 1:4000 (Southern Biotech #2040-05). 5 washes of TBST were done followed by development using 1-Step Ultra TMB-ELISA Substrate Solution (ThermoFisher Scientific ...
-
bioRxiv - Immunology 2022Quote: ... Plates were washed with water three times before applying 25 μl of secondary antibody (goat anti-human IgG Fc-Southern Biotech, 2048-04 ...
-
bioRxiv - Microbiology 2022Quote: ... the beads were incubated with 1 µg/mL Alexa Fluor647-conjugated goat F(ab’)₂ anti-human kappa (Southern Biotech, 2062-31) in PBS-TH for 30 min at 4 °C with shaking ...
-
bioRxiv - Microbiology 2021Quote: ... Plates were washed three times and incubated with the goat anti-human-IgG Fc secondary antibody conjugated with alkaline phosphatase (AP, Southern Biotech) at a 1:1000 dilution in blocking buffer for 1 h at room temperature ...
-
bioRxiv - Systems Biology 2022Quote: ... for capture and biotinylated anti-human insulin antibody (Eli Lilly and Company) with horseradish peroxidase conjugated streptavidin (7100-05, Southern Biotech) for detection ...
-
bioRxiv - Immunology 2022Quote: ... The plates were then washed with PBS containing 0.1% Tween-20 (PBS-T), and Alkaline Phosphatase-conjugated Goat Anti-Human IgG (dilution 1:500, catalog no. 2040-04) from Southern Biotech was added and incubated for 45 min at room temperature ...
-
bioRxiv - Immunology 2019Quote: ... washed 6x 300μl PBST and a 1/5000 cocktail of anti-human kappa-HRP and lambda-HRP antibodies (SouthernBiotech, Birmingham, AL) added for 1 hour in assay diluent ...
-
bioRxiv - Immunology 2019Quote: ... Plates were washed with PBS/0.1% Tween 20 and wells were incubated with alkaline phosphatase-conjugated anti-human Fc (Southern Biotech; P7998) followed by 2 hour incubation ...
-
bioRxiv - Immunology 2020Quote: ... Plates were washed 4X in wash buffer and the secondary antibody Goat anti-Human Ig-HRP (Southern Biotech, Cat# 2010-05), Peroxidase-conjugated AffiniPure Donkey Anti-Human IgG ...
-
bioRxiv - Immunology 2019Quote: ... Cells were washed with PBS–0.1% Tween 20 and incubated with mouse anti-human IgG Fc-HRP (Southern Biotech; 9040-05) for 1 hour ...
-
bioRxiv - Immunology 2019Quote: ... Plates were washed with PBS–0.1% Tween 20 and incubated with mouse anti-human IgG Fc-HRP (Southern Biotech; 9040-05) for 1 hour ...
-
bioRxiv - Microbiology 2020Quote: ... the peptide arrays were incubated with a 1/1000 dilution of an appropriate antibody peroxidase conjugate (goat anti-human HRP conjugate, Southern Biotech, cat ...
-
bioRxiv - Immunology 2021Quote: ... incubated for 1 hour at room temperature with mouse anti-human IgG conjugated to horse radish peroxidase (Southern Biotech, Birmingham, USA) diluted 1:5000 in blocking solution ...
-
bioRxiv - Cell Biology 2020Quote: ... Mouse/Human ads-HRP (4050-05, dilution 1:10000) and Goat Anti-Mouse IgG(H+L) Human ads-HRP (1031-05, dilution 1:10000) (Southern Biotech); sheep anti-TGN38 (AHR499G ...
-
bioRxiv - Immunology 2022Quote: ... The cells were then stained with 50 μL of the secondary mix (goat anti-human IgG-AF488 (SouthernBiotech, cat. #2040-30) and goat anti-mouse Alexa Fluor 488 IgG (H + L ...
-
bioRxiv - Immunology 2022Quote: ... The microtiter plates were developed with horseradish peroxidase (HRP)-labeled goat anti-human IgG or IgM polyclonal antibodies (SouthernBiotech, Birmingham, AL). The plates were developed with 3,3 ...
-
bioRxiv - Immunology 2022Quote: ... The plates were then washed three times with water and 200 μl of goat anti-human IgG HRP secondary antibody (Southern Biotech) diluted to a ratio of 1:2000 in block buffer was added and incubated for 1 hr at room temperature followed by an hour of incubation ...
-
bioRxiv - Cell Biology 2023Quote: ... as well as FITC- and Texas Red-conjugated goat polyclonal anti-human lambda-light chain (cat. 2070-02 and 2070-07) were obtained from Southern Biotech and were used for immunofluorescent microscopy ...
-
bioRxiv - Immunology 2023Quote: ... Cells were washed and stained with AF647-conjugated goat anti-human IgG or IgA as appropriate (2050-31 and 2040-31, Southern Biotech) and analyzed by flow cytometry as described above.
-
bioRxiv - Immunology 2023Quote: ... Bound IL-21 was subsequently detected with mouse anti-human IL-21-biotin (clone 2B2-G20, eBioscience) followed by alkaline phosphatase-conjugated streptavidin (Southern Biotech). The signals were compared to a standard curve generated with recombinant human IL-21 (R&D Systems).
-
bioRxiv - Microbiology 2024Quote: ... Concentrations were evaluated by linear regression analysis built by potting OD405 values against a standard curve generated by titration of a human IgG-unlabeled antibody (Southern Biotech).
-
bioRxiv - Immunology 2023Quote: ... Beads were then washed with PBST and reacted for 30 minutes at room temperature with biotinylated goat anti-human IgG (Southern Biotech) or goat whole IgG containing anti-monkey IgA (Novus ...
-
bioRxiv - Molecular Biology 2020Quote: ... Slides were mounted with DAPI Fluoromount-G (DAPI, 4,6-diamidino-2-phenylindole) (SouthernBiotech). Stained slides were imaged using Leica Application Suite X software on a Leica Sp8 (Leica) ...
-
bioRxiv - Neuroscience 2021Quote: ... The slides were mounted using 4,6-diamidino-2-phenylindole (DAPI) Fluoromount-G (SouthernBiotech) and sealed with a coverslip ...
-
bioRxiv - Neuroscience 2022Quote: ... then 2 times with 1X PBS before mounting with Fluoromount-G (Southern Biotech) on slides with a 0.5mm spacer between the slide and coverslip ...
-
bioRxiv - Bioengineering 2022Quote: ... We washed with PBS three times to remove the unbound fractions and treated the mixtures with anti-human IgG secondary antibody (SouthernBiotech #2016-05). The plate was further incubated at room temperature for 30 min ...
-
bioRxiv - Microbiology 2020Quote: ... washed 3 times in ice-cold PBSM and then bound antibody detected with goat anti-human kappa-A647 (SouthernBiotech, cat# 2060-31) After washing ...
-
bioRxiv - Immunology 2020Quote: ... The plates were washed twice in FACS buffer and stained with 50 μl/well of 1:200 dilution of R-phycoerythrin (PE)-conjugated mouse anti-human IgG Fc antibody (SouthernBiotech #9040-09) and 1:1000 dilution of Zombie-NIR viability dye (BioLegend ...
-
bioRxiv - Immunology 2022Quote: ... the cells were stained with 50μl/well of 1:200 diluted R-phycoerythrin (PE)-conjugated mouse anti-human IgG Fc antibody (SouthernBiotech cat.# 9040-09) and 1:1000 dilution of Zombie-NIR viability dye (BioLegend cat.# 423105 ...
-
bioRxiv - Immunology 2023Quote: ... IgG3 was measured following 6 washes and a 1-h incubation with 100 μL/well 1:5,000 mouse anti-human biotin-conjugated IgG3 hinge (SouthernBiotech, Birmingham, Alabama, USA) then 6 washes and 1-h incubation with 100 μL/well 1:40,000 HRP-conjugated streptavidin (Jackson ImmunoResearch Labs) ...
-
bioRxiv - Immunology 2023Quote: ... MAB273 was detected by adding a 1:5,000 dilution of monkey cross-adsorbed polyclonal goat anti-human IgG HRP antibody (Southern Biotech, 2049-05) and the signal was developed as described above.
-
bioRxiv - Immunology 2021Quote: ... Plates were washed 5 times with PBS/0.05% Tween20 and IgG antibodies were detected using horseradish peroxidase (HRP)-conjugated goat anti-human IgG detection antibody (Southern Biotech, Birmingham, AL, USA) at a 1:4,000 dilution and incubated for 90 min at 37°C ...
-
bioRxiv - Immunology 2021Quote: ... Unbound antibodies were washed away with PBST and bound antibodies detected with 0.5 μg/mL HRP-conjugated goat anti-human constant kappa (2060-05, Southern Biotech, Birmingham, AL, USA) diluted in PBSTB ...
-
bioRxiv - Microbiology 2021Quote: ... incubated with primary antibody (starting concentration 10 µg/ml) in PBS for 120 min and secondary antibody (anti-human IgG-HRP; Southern Biotech 2040-05) diluted 1:2500 in 1% BSA in PBS for 60 min at RT ...
-
bioRxiv - Immunology 2020Quote: ... with excess of PBS-T before being incubated for one hour at RT with an alkaline phosphatase-conjugated goat anti-human IgG (Southern Biotech, Birmingham, AL), diluted 3000 × in PBS-T ...
-
bioRxiv - Immunology 2020Quote: ... the plates were first washed 5 times with PBS-T and then incubated for 1 hour at RT with HRP-conjugated anti-human IgG (Fc-specific, clone JDC-10, Southern Biotech, Birmingham, AL) diluted 3000 × in PBS-T ...
-
bioRxiv - Immunology 2020Quote: ... the plates were washed at least 5 times with PBS-T and then incubated for 1 hour at RT with HRP-conjugated anti-human IgG (Fc-specific, clone JDC-10, Southern Biotech, Birmingham, AL) diluted 3000 × in PBS-T ...
-
bioRxiv - Immunology 2021Quote: ... plates were washed with PBS-T and HRP-conjugated goat anti-mouse IgG or goat anti-human IgG (Southern Biotech, Birmingham, AL, USA) added for 1 hour ...