Labshake search
Citations for SouthernBiotech :
301 - 336 of 336 citations for 3 hydroxy 20 oxopregn 5 en 21 yl acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... at 1×106 cells/well and stimulated for 48 hours with 5 μg/mL of RBD-His or hCD81-His or media alone (RPMI + 5% FCS) in the presence of anti-mouse CD28 antibody [0,5 μg/ml] (Southern Biotech). As positive control cells were stimulated with anti-mouse CD3 [0,05 μg/ml] (Southern Biotech) ...
-
bioRxiv - Immunology 2021Quote: ... Plates were washed 5 times with PBS-T and incubated with goat anti-mouse IgG1 antibody conjugated with horseradish peroxidase (SouthernBiotech) for 1 hour at room temperature ...
-
bioRxiv - Immunology 2024Quote: ... Membranes were incubated in TBST containing 5% (w/v) skimmed milk with goat anti-mouse IgG conjugated to horseradish peroxidase (HRP) (1:2000, 1010-05, SouthernBiotech) for 3h at 4°C in a rotary platform and washed thrice with TBST for 10 min each time ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were washed three times in 1X PBS for 5 min before being mounted on slides with Fluoromount-G (SouthernBiotech).
-
bioRxiv - Immunology 2024Quote: ... they were washed (6 × 5 min) with T-PBS 0.1% and incubated with streptavidine coupled with alkaline phosphatase (Southern Biotech) diluted 2000 × ...
-
bioRxiv - Immunology 2024Quote: ... Samples were resuspended in 100 µL binding buffer supplemented with 5 µL of Annexin V-FITC (Cat#: 10040-02, SouthernBiotech) and stained for 20 minutes at room temperature ...
-
bioRxiv - Neuroscience 2024Quote: ... sections were washed three times in 0.4% Triton X-100 in PBS for 5 min and coverslipped with Fluoromount-G (Southern Biotech) containing 0.5µg/mL of DAPI (Roche) ...
-
bioRxiv - Neuroscience 2024Quote: Cochleas were dissected into 4-5 flat turns and mounted on slides using DAPI-Fluoromount-G mounting medium (Southern Biotech) for imaging ...
-
bioRxiv - Immunology 2021Quote: ... cells were resuspended in calcium-containing HBSS and incubated at room temperature for 5 minutes before activation by anti-IgM F(ab′)2 (Southern Biotech) or VLPs ...
-
bioRxiv - Neuroscience 2020Quote: ... the sections were then rinsed again three to four times in PBS for 5-10 min and mounted on Fisherbrand microscope slides and cover slipped using Fluoromount-G (Southern Biotech) mounting medium.
-
bioRxiv - Neuroscience 2020Quote: ... All coverslips were then washed three times (five minutes per wash) in 1xPBS followed by block and permeabilization with 5% (v/v) normal goat serum (NGS, Southern Biotech) and 0.25% (v/v ...
-
bioRxiv - Neuroscience 2020Quote: ... Slices were subsequently washed three times for 5 min each in PBS and mounted on microscope slides (Brain Research Laboratories) using Fluoromount-G mounting medium (Southern Biotech). Images were obtained with an EVO FL Imaging System equipped with 4x objective to visualize entire ACA with the adjacent cortical areas simultaneously ...
-
bioRxiv - Physiology 2020Quote: ... Sections were again washed 3 times 5 minutes with 1x PBS followed by mounting with coverslips using Fluoromount-G mounting media containing DAPI nuclear stain (Southern Biotech).
-
bioRxiv - Immunology 2020Quote: ... plates were washed 4 times with PBS-T and an anti-monkey IgG conjugated to horseradish peroxidase (Southern Biotech 4700-5) 1 hour at 37°C ...
-
bioRxiv - Microbiology 2023Quote: ... Alexa Fluor® Plus 488 secondary antibodies were diluted 1:1,000 in the respective blocking solutions before mounting with 5 µL DAPI Fluoromount-G® (Southern Biotech) and imaging ...
-
bioRxiv - Biochemistry 2022Quote: ... labeled protein was visualized using the AP substrate 5-bromo-4-chloro-indolyl-phosphate/nitro blue tetrazolium (BCIP/NBT; Southern Biotech).
-
bioRxiv - Immunology 2022Quote: ... NP-OVA specific IgG1 was detected as previously described48 using plates were coated with TNP(5)-BSA and detected using goat anti-mouse IgG1-HRP (Southern Biotech).
-
bioRxiv - Physiology 2023Quote: ... Non-specific antibody binding was blocked for 1 hour at room temperature with a blocking solution containing: 5% normal goat serum (006001, SouthernBiotech™), 0.3% Triton™ X-100 (X100 ...
-
bioRxiv - Molecular Biology 2024Quote: ... EPS concentration was established using anti-GXM IgG 18B7 at 5 µg/ml 50 µl/well and goat anti-mouse IgG1 + alkaline phosphatase (Southern Biotech) in the 96-well plate (CORNING) ...
-
bioRxiv - Immunology 2024Quote: ... Sections were washed three times with PBS (5 min each at RT, ∼90rpm) before mounting in Fluoromount G with or without DAPI (Southern Biotech). Any antibodies directly conjugated to fluorophores were incubated with primary antibodies overnight ...
-
bioRxiv - Physiology 2024Quote: ... Sections were again washed 3 times 5 minutes with 1x PBS followed by mounting with coverslips using Fluoromount-G mounting media containing DAPI nuclear stain (Southern Biotech). CD68 staining was performed according to the Cell Signaling protocol for antibody #97778 ...
-
bioRxiv - Bioengineering 2024Quote: ... or a 1:1 mixture of anti-mouse kappa and anti-mouse lambda capture antibodies at 5 µg/mL (for standards; Southern Biotech) in PBS at 4 °C overnight ...
-
bioRxiv - Cell Biology 2022Quote: ... Coverslips with stained cells were washed 2 x 5 min in PBS and then mounted with Fluoromount-G (Southern Biotech 0100-01) for image acquisition ...
-
bioRxiv - Cancer Biology 2020Quote: ... All cells were maintained at 37 ◻C with 5% CO2 and were mycoplasma free as determined a PCR assay (Southern Biotech).
-
bioRxiv - Biochemistry 2020Quote: ... for 1 min, washed with PBS again (3X, 5 min) and slides were mounted with Fluoromount-G mounting medium (SouthernBiotech, Birmingham, AL). Images were acquired on a Leica SP5 confocal microscope and analyzed by Fiji ImageJ.
-
bioRxiv - Microbiology 2023Quote: ... cells were attached to a poly-L-lysine treated slide before 5 μl of Fluoromount G with DAPI (Cambridge Bioscience, Southern Biotech) was added and coverslip applied ...
-
bioRxiv - Cancer Biology 2024Quote: ... brain slices were washed four times with PBST for 5 minutes each and then mounted on a glass slide in mounting medium (SouthernBiotech, 0100-01), covered with glass coverslips ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells were then washed with PBS-BSA and stained with secondary antibodies (5 μM Alexa Fluor® 488 anti-mouse (SouthernBiotech; 1031-30) and 10 μM Alexa Fluor® 647 anti-human (SouthernBiotech ...
-
bioRxiv - Biochemistry 2020Quote: ... the plate was washed extensively (5×) with PBS-T and incubated with a HRP-conjugated mouse anti-human IgG (9040-05, clone JDC-10, Southern Biotech, Birmingham, AL) for 1 h at room temperature ...
-
bioRxiv - Neuroscience 2022Quote: ... The sections were then washed a final time with PBST 5 times for 10 min per wash and then mounted with Fluoromount-G (SouthernBiotech, catlog # OB100-01).
-
bioRxiv - Neuroscience 2024Quote: ... The specimens were subjected to a triple wash with PBS and treated with a 5% normal donkey serum (NDS; Southern Biotech, #0030-01) solution in PBST (PBS with 0.1% Triton X ...
-
bioRxiv - Immunology 2023Quote: ... primary B cells (107/ml) were stained for 5 min on ice with 10 μg/ml of biotinylated anti-IgM (Southern Biotech, 1021-08) and washed with Imaging Buffer ...
-
bioRxiv - Immunology 2023Quote: ... sonicated to disrupt bead:bead aggregates (Bioruptor®: HIGH, 5 cycles, 30” on/off) and coated with different ratios of biotinylated anti-IgM (Southern Biotech, #1021-08) and biotinylated Eα52-68 peptide (Biotin-GSGFAKFASFEAQGALANIAVDKA-COOH ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells were then fixed in 4% paraformaldehyde in Hank’s balanced salt solution (HBSS) for 30 min at room temperature and washed 5 times in HBSS before mounting using Fluoromount with DAPI (Southern Biotech, Birmingham, AL).
-
bioRxiv - Neuroscience 2024Quote: ... Cells were then fixed in 3.7% paraformaldehyde in Hank’s balanced salt solution (HBSS) for 30 min at room temperature and washed 5 times in HBSS before mounting using Fluoromount with DAPI (Southern Biotech, Birmingham, AL). Images were acquired using a Zeiss LSM 880 inverted confocal microscope (Carl Zeiss ...
-
bioRxiv - Immunology 2023Quote: ... Membranes were washed with TBST (1x, 5 min., RT) and incubated with goat anti-rabbit IgG- HRP (Southern Biotech #1021-05, 10 mL, 1:5,000, 1 h, RT). Membranes were washed with TBST (3x ...