Labshake search
Citations for SouthernBiotech :
251 - 300 of 371 citations for PRG2 Protein Human Recombinant His Tag HPLC verified since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... Cells were washed with 1x PBS and incubated with 1:4000 anti-human IgG-HRP (Southern Biotech) for 1h at 37°C ...
-
bioRxiv - Synthetic Biology 2024Quote: ... the cells were incubated with an anti-human IgG antibody conjugated to Alexa Fluor-488 (Southern Biotech Catalog# 2015-30 ...
-
bioRxiv - Microbiology 2020Quote: ... Bound antibodies were detected with horseradish peroxidase (HRP)-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech) in 1% BSA in PBS-T and Tetramethylbenzidine (TMB ...
-
bioRxiv - Immunology 2021Quote: ... 25 μl of 1:5000 diluted horse radish peroxidase (HRP) conjugated anti-Human IgG secondary antibody (Southern Biotech) was added and incubated for 1h at RT ...
-
bioRxiv - Microbiology 2020Quote: ... Antibody binding to the peptides was determined using a goat anti-human IgG HRP conjugate (ITK Southern Biotech) for 1 h at RT and tetramethylbenzidine substrate (BioFX) ...
-
bioRxiv - Biochemistry 2021Quote: ... and the secondary antibody solution was a 1:1200 dilution of goat-anti-human IgG Fc-HRP (SouthernBiotech). ELISA curves were fit to a 4-parameter logistic curve (Fig ...
-
bioRxiv - Microbiology 2020Quote: ... Plates were washed three times and incubated with HRP-conjugated goat anti-human secondary antibody (ITK Southern Biotech) diluted 1:2000 in blocking buffer for 1 hour at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... and then incubated with either goat anti-human IgG-HRP conjugated antibodies (Southern Biotech, catalog no. 2040-05), goat anti-human IgA-HRP conjugated antibodies (Southern Biotech ...
-
bioRxiv - Immunology 2020Quote: ... was coated with a 1:500 dilution of unlabeled goat anti-human IgG (Southern Biotech, Birmingham, AL, USA) and incubated at 37°C for 1 h ...
-
bioRxiv - Immunology 2021Quote: ... Total antigen-specific IgG was then detected with a mouse anti-human IgG phycoerythrin-conjugated Ab (Southern Biotech) at 2 μg/mL ...
-
bioRxiv - Cancer Biology 2020Quote: ... with 10 μg/mI F(ab’)2 Anti-human anti-IgM-UNLB (#2022-01, Southern Biotech, Birmingham, AL) resuspended in PBS ...
-
bioRxiv - Immunology 2023Quote: ... Cells were either left unstimulated or 20 µg/mL of F(ab’)2 anti-human IgM (Southern Biotech) was added directly to the well ...
-
bioRxiv - Immunology 2023Quote: ... cells were either left unstimulated or 20 µg/mL of F(ab’)2 anti-human IgM (Southern Biotech) was added directly to the well ...
-
bioRxiv - Immunology 2023Quote: ... and incubated with 0.75x serial dilutions of purified polyclonal human antibodies (IgG, SouthernBiotech, Cat # 0150-01; IgM, SouthernBiotech, Cat # 0158L-01 ...
-
bioRxiv - Immunology 2022Quote: ... beads were incubated for 1 hr with PE-conjugated mouse anti-human IgG antibodies (JDC-10, Southern Biotech). After three washes ...
-
bioRxiv - Immunology 2023Quote: ... Cells were either left unstimulated or 20 µg/mL of F(ab’)2 anti-human IgM (Southern Biotech) was added directly to the well ...
-
bioRxiv - Immunology 2022Quote: ... and all the positive controls were detected with an HRP-conjugated polyclonal goat anti-human IgG (Southern Biotech) by 1 hour incubation at room temperature ...
-
bioRxiv - Biophysics 2021Quote: ... Both horseradish peroxidase (HRP) conjugated anti-human kappa and anti-rabbit IgG were purchased from SouthernBiotech (Birmingham, AL, USA) and Abcam (Cambridge ...
-
bioRxiv - Microbiology 2021Quote: ... samples were diluted at 1:50 and antigen-coupled microspheres were incubated with secondary antibody diluted at 1:100 each: mouse anti-human IgG1 hinge (0.1 mg/ mL, clone 4E3, SouthernBiotech); IgG2 Fc (0.1 mg/ mL ...
-
bioRxiv - Microbiology 2021Quote: ... but the secondary antibody used was an anti-human IgM Peroxidase-labelled antibody produced in goats (SouthernBiotech, Birmingham, USA).
-
bioRxiv - Immunology 2021Quote: ... IgG capture beads were generated by the direct conjugation of goat anti-Human IgG F(ab)2 (Southern Biotech) to array particles (Spherotech ...
-
bioRxiv - Cell Biology 2021Quote: ... dilution 1:10,000) and Goat Anti-Mouse IgG (H + L) Human ads-HRP (1031-05, dilution 1:10,000) (Southern Biotech); sheep anti-TGN38 (AHP499G ...
-
bioRxiv - Immunology 2020Quote: ... CMV glycoprotein–specific antibody binding was detected with phycoerythrin-conjugated goat anti-human IgG (2 μg/mL, Southern Biotech). Beads were washed and acquired on a Bio-Plex 200 instrument (Bio-Rad) ...
-
bioRxiv - Microbiology 2020Quote: ... followed by staining with goat F(ab’)2 anti-human kappa-Alexa Fluor 647 (5 μg/ml, Southern Biotech) (Supporting figure 1A).
-
bioRxiv - Immunology 2021Quote: ... The cells were washed with FACS buffer and incubated with secondary goat anti-human IgG PE antibody (Southern Biotech) for 1 hour at 4°C ...
-
bioRxiv - Immunology 2023Quote: ... At various time points after addition of 20 µg/mL of F(ab’)2 anti-human IgM (Southern Biotech), cells were then labeled by addition of hydrogen peroxide (H2O2 ...
-
bioRxiv - Immunology 2023Quote: ... antibody response to individual peptides was detected with different fluorescently labeled secondary antibodies (Goat anti-human IgM-AF488, SouthernBiotech, Cat # 2020-30 ...
-
bioRxiv - Microbiology 2023Quote: ... followed by staining with goat F(ab’)2 anti-human kappa-Alexa Fluor 647 (5 μg/mL, Southern Biotech), fixation in PBS 1% formaldehyde and analysis by flow cytometry (BD FACSVerse).
-
bioRxiv - Microbiology 2024Quote: ... Antibodies used for immunoblot analysis in this study were: anti-Human IgM goat polyclonal antibody (Southern Biotech #2020-01), anti-Human IgG goat polyclonal antibody (Southern Biotech #2040-01) ...
-
bioRxiv - Immunology 2023Quote: ... PE conjugated goat anti-human IgG Fc secondary Ab was added at 6μg/ml (Southern Biotech, catalog: 2014-09) and incubated for 1 hour at 37°C with shaking at 700 rpm ...
-
bioRxiv - Immunology 2022Quote: ... The secondary antibody solution was prepared by diluting goat anti-human IgG HRP-conjugated antibodies (SouthernBiotech, cat. # 2040-05) at 1:2,000 in blocking buffer and adding 20 to 25 μL/well ...
-
bioRxiv - Immunology 2023Quote: ... Samples were either left untreated or treated with 20 µg/mL F(ab’)2 anti-human IgM (Southern Biotech). 48 hours later ...
-
bioRxiv - Microbiology 2024Quote: ... beads were incubated for 1 hour at room temperature with Phycoerythrin-conjugated goat anti-human IgG antibodies (Southern Biotech). After three washes ...
-
bioRxiv - Immunology 2024Quote: ... PE conjugated goat anti-human IgG Fc secondary Ab was added at 6μg/mL (Southern Biotech, catalog: 2014-09) and incubated for 1h at 37°C with shaking at 700 rpm ...
-
bioRxiv - Synthetic Biology 2024Quote: ... The blot was then incubated for 1 h at room temperature with 0.5 µg/mL of goat anti-human kappa antibody conjugated to HRP (31460, SouthernBiotech) if hSC44.20 ...
-
bioRxiv - Cancer Biology 2020Quote: ... 1 × 105 dendritic cells or B cells were incubated with different concentrations of C03 with or without goat anti-human Fc antibody (SouthernBiotech) for 48 hours ...
-
bioRxiv - Cancer Biology 2020Quote: ... Jurkat/NF-κB-GFP-hCD40 reporter cells were incubated with different concentrations of CD40 agonist antibodies with or without goat anti-human Fc antibody (SouthernBiotech) for 24 hours ...
-
bioRxiv - Immunology 2022Quote: ... Plates were then washed with TBST and incubated with secondary HRP-conjugated goat anti-human IgG (Southern Biotech, Birmingham, AL) at a 1:15,000 dilution for 45 minutes at room temperature ...
-
bioRxiv - Cancer Biology 2022Quote: ... followed by detection with a 1:10,000 dilution of goat anti-human IgG-HRP (Southern Biotech 2040-05, Birmingham, AL). Plates were developed for 5 minutes with TMB substrate (VWR 95059-286) ...
-
bioRxiv - Immunology 2020Quote: ... MagPlex beads were washed with PBST (PBS, 0.05% Tween-20) and incubated with anti-human IgG Fc-PE (Southern Biotech). Finally ...
-
bioRxiv - Immunology 2021Quote: ... the pellet was resuspended in a 50 μL mixture of PE-anti-human CD11b (Southern Biotech, 10 μL/million cells), Alexa Fluor 647-anti-human CD35 (BD Biosciences ...
-
bioRxiv - Immunology 2020Quote: ... bound IgG was detected by incubating with 1:1000 polyclonal goat anti human IgG-HRP (Southern Biotech, Birmingham, AL, USA) for 1h at 37°C ...
-
bioRxiv - Immunology 2020Quote: ... washed thrice with PBST and detected with HRP-conjugated mouse anti-human IgG (Fc only) (Southern Biotech, Birmingham, AL, USA) antibody at a 1:2000 dilution ...
-
bioRxiv - Immunology 2020Quote: ... Plates were washed three times and incubated with horseradish peroxidase (HRP)-conjugated goat anti-human secondary antibody (ITK Southern Biotech) diluted 1:2000 in blocking buffer for 1 hour at RT ...
-
bioRxiv - Microbiology 2024Quote: ... Antibodies used for flow cytometry analysis performed in this study were: FITC-conjugated anti-human IgM (Southern Biotech #2020-02), goat anti-human IgG-UNLB (Southern Biotech #2040-01) ...
-
bioRxiv - Biochemistry 2024Quote: ... PVDF membranes containing blotted proteins were washed in excess PBS-Tween 20 for 1h at room temperature before application of a 1:2000 dilution of primary mouse Anti-Human IgA1 antibodies conjugated to biotin (SouthernBiotech). Blots were washed 10 min 3 x in PBS followed by treatment with a 1:2000 dilution of ExtrAvidin®−Peroxidase conjugate (Sigma ...
-
bioRxiv - Microbiology 2023Quote: ... was used except that plates were coated with anti-human kappa and lambda light chain specific mouse antibodies (Southern Biotech) at 1:1 ratio diluted to 1 μg/ml in PBS overnight ...
-
bioRxiv - Immunology 2022Quote: ... plates were washed with TBS containing 0.05% Tween-20 (TBST) and resuspended in 50 μL of Goat-anti-human IgG-PE (Southern Biotech). After 2 hours of incubation on a rotator at room temperature ...
-
bioRxiv - Immunology 2022Quote: ... IgG antibodies were detected using horseradish peroxidase (HRP)-conjugated goat anti-human IgG detection antibody (Southern Biotech, Birmingham, AL, USA) at a 1:4,000 dilution and colorimetric development was accomplished using 100 μL of 0.1% 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid ...
-
bioRxiv - Immunology 2024Quote: ... bound antibodies were detected by incubation for 1 hour at room temperature with HRP-conjugated goat anti-human IgG (diluted 1:3000 in PBS-T; SouthernBiotech), IgM (1:1500 ...