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Citations for SouthernBiotech :
101 - 150 of 832 citations for Son of sevenless homolog 1 SOS1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... followed by the addition of horseradish peroxidase-conjugated goat anti-mouse-IgG Fc secondary antibody (1:2000) (Southern Biotech, 1033-05). After incubation for 2 hours at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... the peptide arrays were incubated with a 1/1000 dilution of an appropriate antibody peroxidase conjugate (goat anti-human HRP conjugate, Southern Biotech, cat ...
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bioRxiv - Microbiology 2021Quote: ... Fab fragments were detected with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech, 1:500). After washing ...
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bioRxiv - Microbiology 2024Quote: ... Following additional washings and incubation at 37°C for 1 h with AP-conjugated anti-goat IgG secondary antibody (Southern Biotech), absorbance was read by addition of PNPP ...
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bioRxiv - Microbiology 2023Quote: ... Alexa Fluor® Plus 488 secondary antibodies were diluted 1:1,000 in the respective blocking solutions before mounting with 5 µL DAPI Fluoromount-G® (Southern Biotech) and imaging ...
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bioRxiv - Cell Biology 2023Quote: ... Labeling of primary antibodies was visualized with anti-mouse PE and anti-rat PE (both Southern Biotech, USA, 1:1000 diluted). Actin in living cells was visualized with SiR actin (Spirochrome ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies conjugated with HRP used in the study are: Goat Anti-mouse IgG1-HRP (1:10000, Southern Biotech, 1071-05), Goat Anti-rabbit IgG-HRP (1:10000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Precomplexation of the anti-sialyl-LewisX antibody was performed with a 1:50 dilution of goat anti-mouse IgM-HRP (#1021-05, Southern Biotech) and 0.65 µg donkey anti-goat Alexa Fluor 555 (A21432 ...
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bioRxiv - Molecular Biology 2023Quote: ... and primary antibodies were added in 1 : 10000 dilution (anti-IgG Fc cat. 31789, Thermo Fisher; anti-His-Tag cat. 4603-08, SouthernBiotech) followed by 1 h incubation at RT ...
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bioRxiv - Immunology 2022Quote: ... IgG was detected using phycoerythrin-conjugated mouse antibodies against human antibody subclasses (Southern Biotech). Secondary incubations were performed over 2 hours ...
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bioRxiv - Immunology 2022Quote: ... IgG2b or IgG3 antibody (SouthernBiotech) (to determine antibody isotype ...
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bioRxiv - Immunology 2022Quote: ... and IgA antibodies (Southern Biotech) used at a concentration of 10 μg/ml in PBS ...
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A balance between pro-inflammatory and pro-reparative macrophages is observed in regenerative D-MAPSbioRxiv - Bioengineering 2023Quote: ... antibodies were utilized (Southern Biotech) in place of the total IgG detection antibody while all other steps were similar ...
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Exploring the Role of Spatial Confinement in Immune Cell Recruitment and Regeneration of Skin WoundsbioRxiv - Bioengineering 2023Quote: ... antibodies were utilized (Southern Biotech) in place of the total IgG detection antibody while all other steps were similar ...
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bioRxiv - Immunology 2024Quote: ... and IgE antibodies (Southern Biotech) with a TMB solution (Biolegend) ...
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bioRxiv - Biochemistry 2021Quote: ... 1×107 cells were starved for 30 min at 37°C in 1 ml FCS-free RPMI and then treated with 10 µg/ml anti-λ antibody (Southern Biotech) under shaking at 750 rpm for the indicated time points ...
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bioRxiv - Cell Biology 2020Quote: ... samples were incubated for 30 minutes with the following secondary antibodies diluted in PBS 2.5% BSA: donkey anti-goat Alexa Fluor 488 1:200 (Southern Biotech 3425-30) and donkey anti-rabbit Alexa Fluor 555 1:200 (Thermo fisher scientific A32794) ...
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bioRxiv - Microbiology 2020Quote: ... the wells were incubated for 2 h at 37°C with HRP-mouse anti-human IgG antibody (dilution 1:2,000) (Southern Biotech, Birmingham, AL) followed by the peroxidase substrate ...
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bioRxiv - Developmental Biology 2022Quote: ... then incubated in fresh dilution solution with secondary antibody (goat anti-mouse IgG2a AlexaFluor 488, 1:250, Southern Biotech, cat# 1080-30) overnight at 4°C with gentle shaking ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed twice in FACS buffer and stained with 50 μl/well of 1:200 dilution of R-phycoerythrin (PE)-conjugated mouse anti-human IgG Fc antibody (SouthernBiotech #9040-09) and 1:1000 dilution of Zombie-NIR viability dye (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... Plates were washed with PBS + 0.05% Tween20 and binding was detected with anti-kappa HRP antibody (1:5000, Southern Biotech #2060-05) and developed with the TMB substrate (KPL (Mandel ...
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bioRxiv - Immunology 2022Quote: ... the cells were stained with 50μl/well of 1:200 diluted R-phycoerythrin (PE)-conjugated mouse anti-human IgG Fc antibody (SouthernBiotech cat.# 9040-09) and 1:1000 dilution of Zombie-NIR viability dye (BioLegend cat.# 423105 ...
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bioRxiv - Immunology 2024Quote: ... plates were washed four times and 25 µl of a 1:500 dilution of the respective detection antibody (goat anti-mouse IgG(H+L)-alkaline phosphatase (AP) conjugate (Southern Biotech/Biozol) or goat anti-mouse IgG1-AP (Southern Biotech/Biozol ...
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bioRxiv - Immunology 2023Quote: ... MAB273 was detected by adding a 1:5,000 dilution of monkey cross-adsorbed polyclonal goat anti-human IgG HRP antibody (Southern Biotech, 2049-05) and the signal was developed as described above.
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bioRxiv - Bioengineering 2023Quote: ... followed by 1 hr incubation with horseradish peroxidase-labeled goat anti-mouse secondary antibody (GAM-HRP, SouthernBiotech Cat#1010-05, RRID: AB_2728714). After washing ...
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bioRxiv - Immunology 2020Quote: ... Detection antibodies were subclass specific goat anti-mouse polyclonal R-PE-conjugated antibodies (Southern Biotech) used at a 1:200 dilution ...
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bioRxiv - Molecular Biology 2021Quote: ... and immunoblotted with indicated primary antibodies and appropriate horseradish peroxidase-conjugated secondary antibodies (Southern Biotech). Primary antibodies to IRE-1 (Cell Signaling Technology) ...
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bioRxiv - Immunology 2023Quote: ... Anti-Vi antibody was detected by AP-conjugated goat anti-mouse secondary antibodies (Southern Biotech) at 1:4000 for IgM ...
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bioRxiv - Cell Biology 2020Quote: ... Then samples were washed three times with 0.1% Triton X-100 in PBS for 5 minutes and were incubated 30 minutes at RT with the following secondary antibody diluted 1:200: goat anti-rabbit Alexa Fluor 488 (Southern Biotech 4050-30), goat anti-mouse Alexa Fluor 488 (Southern Biotech 1030-30 ...
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bioRxiv - Immunology 2021Quote: ... Plates were again washed and then incubated with 1:4000 dilution of horse radish peroxidase (HRP) conjugated antimouse IgG secondary antibody (SouthernBiotech, cat. 1030-05) and incubated for 1h at RT ...
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bioRxiv - Bioengineering 2021Quote: ... The slides were subsequently incubated at room temperature with secondary antibodies: goat anti-rabbit Ig (1:100 dilution for 15 minutes, 4010-05, Southern Biotech, Birmingham, AL) for CD4 and goat anti-rabbit (1:50 dilution for 30 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... Antibodies bound to the thymocytes were detected by Cy5-conjugated goat anti-mouse IgG (1:250 dilution, 1030-15, Southern Biotech, Birmingham, AL) and Alexa Fluor 488-conjugated goat anti-mouse IgM (1:250 dilution ...
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bioRxiv - Immunology 2021Quote: ... For detection of IgG subclasses all serum samples were diluted to 1 total IgG EU and incubated at 37 °C for 1 h prior to detection with Alkaline Phosphatase conjugated anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam) incubated for 1 h at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... all serum samples were diluted to 1 total IgG ELISA unit and then detected with anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam). The results of the IgG subclass ELISA are presented using OD values instead of the ELISA units used for the total IgG ELISA ...
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bioRxiv - Immunology 2022Quote: ... and stained with a 1:1,000 solution of goat anti-human IgG antibodies conjugated to phycoerythrin (PE) (Southern Biotech, Cat# 2040-09; RRID:AB_2795648) at 4°C for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... They were then washed with PBS and incubated for 1 h at room temperature with the appropriate secondary antibody: goat anti-Rabbit IgG-HRP (Southern Biotech, #4030-05) or goat anti-Mouse IgG-HRP (Southern Biotech ...
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bioRxiv - Immunology 2023Quote: ... and the development of ADA in plasma samples were detected using a goat anti-human lambda IgG HRP-conjugated secondary antibody at 1:4000 dilution (Southern Biotech, Birmingham, AL). The plasma ADA levels were reported as endpoint titer ...
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bioRxiv - Immunology 2023Quote: ... The SOSIP-specific IgG response in the serially diluted RM plasma was measured using an anti-monkey IgG horseradish peroxidase (HRP)-conjugated secondary antibody at 1:4000 dilution (Southern Biotech, Birmingham, AL) and concentration of the CH505-SOSIP-specific IgG response was estimated using a standard curve of CH505 T/F SOSIP IgG detection by Rh HIV-specific monoclonal antibody ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated for 1 hour at room temperature in TBSX with 1:1000 donkey fluorescent secondary antibody and mounted on slides using Fluoromount-G (Southern Biotech 0100-01) before coverslipping ...
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bioRxiv - Immunology 2024Quote: ... Blocking and primary sample incubations were one hour at 37°C and secondary antibody incubations with a 1:6,000 dilution of mouse anti-monkey HRP conjugate (clone SB108a, Southern Biotech, cat. 4700-05) were 45 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... the membrane was washed five times in PBS-T before it was incubated with the secondary antibody goat α-rat IgG (H&L)-HRP (1:10,000, Southern Biotech #3050-05) or goat α-mouse IgG (H&L)-HRP (1:10,000 ...
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bioRxiv - Immunology 2024Quote: ... 100 μl per well of 1 μg/l alkaline phosphatase (AP)-conjugated goat anti-human antibodies (Goat Anti-Human kappa-AP, SouthernBiotech, ref 2060-04) was incubated in PBS with 0.05% Tween® 20 for 2 h at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... The secondary antibodies (1:10,000 working concentration) for immunoblot were horseradish peroxidase (HRP)-conjugated goat anti-rat immunoglobulin (Ig) (Southern BioTech Cat#3010-05), HRP-conjugated goat anti-rabbit Ig (Southern BioTech Cat#4010-05) ...
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bioRxiv - Immunology 2022Quote: ... Anti-commensal Ig antibodies were detected with horseradish peroxidase-conjugated antibodies against mouse Ig (Southern Biotech). Plates were developed in the dark with 100 ul of tetramethylenbenzidine per well (KPL) ...
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bioRxiv - Immunology 2022Quote: ... and anti- λ antibodies (Southern biotech) followed by an HRP-conjugated donkey anti-goat IgG antibody (Santa Cruz Biotechnology) ...
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bioRxiv - Immunology 2021Quote: ... or IgG3 unlabeled antibody (Southern Biotech) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... IgM or IgA antibodies (Southern Biotech) were used as detection antibodies ...
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bioRxiv - Immunology 2020Quote: ... washed 6 times and then incubated with 1: 6000 dilution of horseradish peroxidase (HRP) conjugated anti-mouse IgG secondary antibody (Southern Biotech; Birmingham, AL, USA) for 1 h at RT ...
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bioRxiv - Immunology 2021Quote: ... Sections were washed in PBS before incubation with secondary antibodies (Table 3) for 1 hr at room temperature followed by mounting with DAPI containing fluoromount (Southern Biotech, Birmingham, AL, USA). Images were captured with an EVOS FL imaging system (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... Binding of primary antibodies was then measured via HRP-tagged antibodies specific for the relevant species (SouthernBiotech) and visualized by chemiluminescent substrate (Immobilon Classico/ Forte western HRP substrate or Thermo Fisher SuperSignal West Dura Extended Duration Substrate).