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Citations for SouthernBiotech :
101 - 150 of 560 citations for Estradiol Serum ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... All coverslips were then washed three times (five minutes per wash) in 1xPBS followed by block and permeabilization with 5% (v/v) normal goat serum (NGS, Southern Biotech) and 0.25% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... Plates were coated with 1.25µg/mL soluble RBD (produced previously13) and mouse serum Ig was detected using a goat anti mouse Ig coupled to HRP (Southern Biotech) diluted at 1/6000.
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bioRxiv - Cancer Biology 2022Quote: ... Bound serum antibodies were then detected with a goat anti-mouse IgG-HRP secondary antibody (Southern Biotech 1030-05, Birmingham, AL) at 1:10,000 for 1 hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... or IgG purified from Ggta1+/+ or Ggta1-/- mouse serum (60 µg/mL, PBS 2% BSA, wt/vol, 30 min.) followed by anti-mouse IgG-PE (Southern Biotech #1030-09 ...
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bioRxiv - Immunology 2020Quote: ... for detection of αGal and IgG purified from Ggta1+/+ or Ggta1-/- mouse serum (60 µg/mL, PBS 2% BSA, wt/vol, 30 min.) followed by anti-mouse IgG-PE (Southern Biotech #1030-09 ...
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bioRxiv - Cell Biology 2020Quote: ... localization of antibody labeling was detected after a 1 hr incubation with secondary antibody diluted in PBS containing 2% normal goat serum and mounting under a coverslip with DAPI Fluoromount-G (Southern Biotech). PPCs were visualized by fluorescent microscopy and their numbers were assessed qualitatively by visual examination ...
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bioRxiv - Cancer Biology 2024Quote: ... permeabilized with 0.1% Triton X-100 (Fisher Biotech, Hampton, NH, USA), and blocked with PBS supplemented with goat serum (2%, v/v) (Southern biotech) and 0.1% Tween-20 (Thermo Fisher) ...
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bioRxiv - Immunology 2019Quote: ... The plates were then washed and incubated with peroxidase-conjugated goat anti-mouse IgM Fc-specific (Southern Biotech). In differentiation assays ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed three times followed by addition of goat anti-mouse IgG horseradish peroxidase conjugated (Southern Biotech) diluted in blocking solution (1:5000) ...
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bioRxiv - Immunology 2021Quote: ... Plates were then rinsed with PBST and incubated with 3,3’,5,5’-tetramethylbenzidine (TMB, Southern Biotech, Birmingham, AL, USA). Absorbance was measured at 650 nm using an Infinite M200 pro plate reader (Tecan ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed three times and incubated with HRP-conjugated goat anti-human secondary antibody (ITK Southern Biotech) diluted 1:2000 in blocking buffer for 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed three times followed by addition of goat anti-mouse horseradish peroxidase-conjugated IgG (Southern Biotech) diluted in blocking solution (1:5000) ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed 3x with PBS and treated with an AP conjugated anti-mouse IgG antibody (Southern Biotech) for 1.5 hours ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed three times followed by addition of goat anti-mouse IgG horseradish peroxidase conjugated (Southern Biotech) diluted in blocking solution (1:5000) ...
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bioRxiv - Immunology 2021Quote: ... High affinity binding plates were coated with purified Ab (0.5 μg/well) directed against Ig H+L (Southern Biotech) for IgG classes or IgE (BD Pharmingen ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed three times with PBS-T and secondary goat anti-mouse IgG-HRP antibody (Southern Biotech), diluted 1:4000 in ELISA buffer ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed with PBS-Tween (0.05%) and PBS before developing with BCIP/NBT blue substrate (Southern Biotech). Spots were imaged with an ImmunoSpot Analyzer (Cellular Technologies Ltd. ...
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bioRxiv - Immunology 2022Quote: ... Plates were then washed three times with PBS-T and secondary goat anti-mouse IgG-HRP antibody (Southern Biotech), diluted 1:4000 in ELISA buffer ...
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bioRxiv - Immunology 2021Quote: ... 96 well plates (Costar) were incubated with anti-IgM or anti-IgG capturing antibody at 1ug/ml (Southern Biotech) and incubated at 4°C for 18h ...
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bioRxiv - Biochemistry 2020Quote: ... The plate was washed four times with PBS-T and 100 μL of TMB peroxidase substrate solution (SouthernBiotech, USA) was added to each well and incubated for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... the plates were washed with washing buffer twice before adding the HRP-conjugated mouse anti-monkey IgG (Southern Biotech) and incubating at room temperature for 1 hour ...
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bioRxiv - Bioengineering 2024Quote: ... plates were washed with PBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG1 antibodies (Southern Biotech) or HRP-conjugated goat anti-mouse IgG2a-HRP antibodies (Southern Biotech ...
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bioRxiv - Neuroscience 2024Quote: ... coverslips were detached from the plate and mounted onto glass slides using Fluoromount-G medium (Southern Biotech, #0100-01) and imaged with a confocal microscope (Leica SP5) ...
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bioRxiv - Neuroscience 2021Quote: ... sections were washed three times with PBS containing 0.3% Tween-20 (PBST) for 10 min and treated with 5% normal donkey serum (NDS; Southern Biotech, cat#0030-01) in PBST for 1 h at room temperature for blocking ...
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bioRxiv - Immunology 2022Quote: ... ELISA plates were coated with 1 μg/ml recombinant SARS-CoV-2 S protein (43) and serially diluted serum samples were added and detected via anti-monkey IgG-HRP (Southern Biotech, Birmingham, AL). Plates were developed using a TMB substrate (source ...
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bioRxiv - Neuroscience 2023Quote: ... 50 µm mouse brain sections were rinsed in PBS for 5 minutes and blocked with PBS/5% normal goat serum (Southern Biotech, 0060-01) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... The specimens were subjected to a triple wash with PBS and treated with a 5% normal donkey serum (NDS; Southern Biotech, #0030-01) solution in PBST (PBS with 0.1% Triton X ...
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bioRxiv - Immunology 2021Quote: ... plates were washed with PBS-T and horseradish peroxidase (HRP)-conjugated goat anti-hamster IgG (Southern Biotech, Birmingham, AL, USA) or goat anti-ferret IgG (Abcam ...
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bioRxiv - Immunology 2022Quote: ... Plates were then washed with TBST and incubated with secondary HRP-conjugated goat anti-human IgG (Southern Biotech, Birmingham, AL) at a 1:15,000 dilution for 45 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed 3X with PBS and 3X with PBS-T and then anti-mouse IgG/IgA-biotin (Southern Biotech) diluted 1:1000 in PBS-T was added and incubated overnight at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed three times and incubated with horseradish peroxidase (HRP)-conjugated goat anti-human secondary antibody (ITK Southern Biotech) diluted 1:2000 in blocking buffer for 1 hour at RT ...
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bioRxiv - Microbiology 2023Quote: ... was used except that plates were coated with anti-human kappa and lambda light chain specific mouse antibodies (Southern Biotech) at 1:1 ratio diluted to 1 μg/ml in PBS overnight ...
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bioRxiv - Neuroscience 2024Quote: ... Coverslips were detached from the plates and mounted in glass slides with DAPI Fluoromount G mounting medium (SouthernBiotech, #0100-20). We imaged neurons with an epifluorescence microscope (Olympus BX51 ...
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bioRxiv - Pathology 2022Quote: ... anti-collagen 1 (1:200, Southern Biotech, 1310-01), anti-collagen 4 (1:5000 ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 4X in wash buffer and the secondary antibody Goat anti-Human Ig-HRP (Southern Biotech, Cat# 2010-05), was added and incubated at 37°C for 1 hr ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed with water three times before applying 25 μl of secondary antibody (goat anti-human IgG Fc-Southern Biotech, 2048-04 ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed 4X in wash buffer and the secondary antibody Goat anti-Human Ig-HRP (Southern Biotech, Cat# 2010-05), Peroxidase-conjugated AffiniPure Donkey Anti-Human IgG ...
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bioRxiv - Immunology 2019Quote: ... Plates were washed with PBS–0.1% Tween 20 and incubated with mouse anti-human IgG Fc-HRP (Southern Biotech; 9040-05) for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... plates were washed 4 times with PBS-T and an anti-monkey IgG conjugated to horseradish peroxidase (Southern Biotech 4700-5) 1 hour at 37°C ...
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bioRxiv - Immunology 2020Quote: ... The following day the plates were washed with PBS-T and incubated with goat anti-mouse IgG1-HRP (Southern Biotech, USA) at 1:6000 dilution in blocking buffer and left for 1 hour at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Hp-specific IgG1 was detected as previously described47 using plates coated with Hp extract and detected using rat anti-mouse IgG1-HRP antibody (Southern Biotech). NP-OVA specific IgG1 was detected as previously described48 using plates were coated with TNP(5)-BSA and detected using goat anti-mouse IgG1-HRP (Southern Biotech).
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bioRxiv - Immunology 2022Quote: ... NP-OVA specific IgG1 was detected as previously described48 using plates were coated with TNP(5)-BSA and detected using goat anti-mouse IgG1-HRP (Southern Biotech).
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bioRxiv - Immunology 2022Quote: ... The microtiter plates were developed with horseradish peroxidase (HRP)-labeled goat anti-human IgG or IgM polyclonal antibodies (SouthernBiotech, Birmingham, AL). The plates were developed with 3,3 ...
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bioRxiv - Immunology 2022Quote: ... The plates were then washed three times with water and 200 μl of goat anti-human IgG HRP secondary antibody (Southern Biotech) diluted to a ratio of 1:2000 in block buffer was added and incubated for 1 hr at room temperature followed by an hour of incubation ...
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bioRxiv - Immunology 2022Quote: ... After overnight incubation at 37°C and 5% CO2 plates were washed extensively and incubated with HRP-conjugated goat-anti-mouse IgA or goat-anti-mouse IgG (Southern Biotech). Subsequently ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed and incubated with a peroxidase-conjugated goat anti-mouse anti-IgM or anti-IgG antibody (Southern Biotech). After washing ...
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bioRxiv - Immunology 2020Quote: ... plates were subsequently incubated with 0.1 µg/mL HRP-labeled goat anti-mouse κ light chain Ab (Southern Biotech, Birmingham, AL 35260), diluted in assay buffer for 1 hour at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... The plates were washed a second time and incubated for 2 h with Streptavidin-alkaline phosphatase from Southern Biotech (2 µg/mL). The final wash was followed by the addition of Nitro-blue Tetrazolium Chloride/5-bromo-4-chloro 3 ‘indolyphosphate p-toludine salt (NBT/BCIP chromagen ...
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bioRxiv - Immunology 2020Quote: ... except constructs were bound directly to the plate and detected with goat anti-mouse IgG HRP conjugate (Southern Biotech, Birmingham, AL, USA).
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bioRxiv - Immunology 2020Quote: ... plates were washed with PBS-T and HRP-conjugated goat anti-mouse IgG or anti-rat IgG (Southern Biotech, Birmingham, AL, USA) added for 1 hour ...