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Citations for SouthernBiotech :
101 - 150 of 644 citations for 7 Chloro 3 methyl 3H 1 2 3 triazolo 4 5 d pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Membranes were incubated in TBST containing 5% (w/v) skimmed milk with goat anti-mouse IgG conjugated to horseradish peroxidase (HRP) (1:2000, 1010-05, SouthernBiotech) for 3h at 4°C in a rotary platform and washed thrice with TBST for 10 min each time ...
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bioRxiv - Immunology 2023Quote: ... Membranes were washed with TBST (1x, 5 min., RT) and incubated with goat anti-rabbit IgG- HRP (Southern Biotech #1021-05, 10 mL, 1:5,000, 1 h, RT). Membranes were washed with TBST (3x ...
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bioRxiv - Immunology 2021Quote: ... RBC-depleted splenocytes were incubated with 1 μg/mL of goat F(ab’)2 anti-mouse Igκ-biotin (SouthernBiotech) for 30 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... plates were washed 4× with ELISA wash buffer and a 1:4000 dilution of goat anti-mouse IgG (SouthernBiotech, catalog #1030-05) in ELISA blocking buffer was added to each well and incubated at 37°C for 1 hour followed by 4 washes with ELISA wash buffer ...
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bioRxiv - Physiology 2023Quote: ... Non-specific antibody binding was blocked for 1 hour at room temperature with a blocking solution containing: 5% normal goat serum (006001, SouthernBiotech™), 0.3% Triton™ X-100 (X100 ...
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bioRxiv - Microbiology 2023Quote: ... Alexa Fluor® Plus 488 secondary antibodies were diluted 1:1,000 in the respective blocking solutions before mounting with 5 µL DAPI Fluoromount-G® (Southern Biotech) and imaging ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were stimulated with buffer (1% FBS/HBSS) or 10 μg/mL goat F(ab’)2 anti-mouse Igκ (SouthernBiotech) and Indo-1 fluorescence (violet vs blue ...
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bioRxiv - Immunology 2024Quote: ... wells were washed six times with PBS supplemented with 0.1 % Tween20 and incubated for 2 h with 1 μg/mL biotinylated goat α-mouse IgM or IgG1 (SouthernBiotech) at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... 1×109 bacteria were washed with 1 ml ice-cold PBS and stained for 30 min at 4°C with 4 µg of FITC-conjugated anti-mouse IgA antibody (Southern Biotech) in 100 µl of staining buffer ...
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bioRxiv - Biochemistry 2020Quote: ... for 1 min, washed with PBS again (3X, 5 min) and slides were mounted with Fluoromount-G mounting medium (SouthernBiotech, Birmingham, AL). Images were acquired on a Leica SP5 confocal microscope and analyzed by Fiji ImageJ.
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bioRxiv - Microbiology 2020Quote: ... IgG-bound DNP-beads were next incubated with 1 µg/mL Alexa Fluor647-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech) in PBS-TH.
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bioRxiv - Immunology 2022Quote: ... a standard curve for mouse isotype was established for each plate by coating 2 columns with 50 μL goat anti-mouse Ig (1 μg/mL in PBS; Southern Biotech) overnight at 4°C ...
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bioRxiv - Immunology 2019Quote: ... the membrane was re-blocked with 5% BSA in 1x TBST for 2 hours at room temperature before probing with 1:5000 dilution of anti-human IgG-HRP (Southern Biotech) in 5% BSA/1x TBST for 1 hour at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Fab fragments were detected with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech, 1:500). After washing ...
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bioRxiv - Microbiology 2020Quote: ... the wells were incubated for 2 h at 37°C with HRP-mouse anti-human IgG antibody (dilution 1:2,000) (Southern Biotech, Birmingham, AL) followed by the peroxidase substrate ...
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bioRxiv - Microbiology 2022Quote: ... the beads were incubated with 1 µg/mL Alexa Fluor647-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech, 2062-31) in PBS-TH for 30 min at 4 °C with shaking ...
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bioRxiv - Biochemistry 2022Quote: ... and then treated with alkaline phosphatase (AP)-conjugated goat anti-rabbit-IgG (Southern Biotech; 1:8000 in TBST with 2% (w/v) bovine serum albumin) ...
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bioRxiv - Neuroscience 2023Quote: ... They were washed six times in washing buffer (PBS with 0.1% Triton X-100) for 20 min and blocked for 1 h in washing buffer containing 5% heat-inactivated goat serum (SouthernBiotech, Cat. No. 0060-01), followed by incubation with the primary antibodies (rabbit anti–GFP ...
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bioRxiv - Immunology 2021Quote: ... IgA (Clone 11-44-2, Southern Biotech), and IgM (Clone RMM-1 ...
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bioRxiv - Bioengineering 2022Quote: ... 5 µL of blocking antibody (anti-IgG, Southern Biotech) was added to the 50 µL cell solution to yield a final concentration of 25 µg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... 50 µm mouse brain sections were rinsed in PBS for 5 minutes and blocked with PBS/5% normal goat serum (Southern Biotech, 0060-01) for 2 hours at room temperature ...
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bioRxiv - Genomics 2024Quote: ... incubating overnight at 4°C in DAPI fluoromount-G (Southern Biotech) and then mounting and imaging on a confocal laser microscope (Leica Sp8).
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bioRxiv - Biophysics 2023Quote: ... we washed the samples 5 times for 5 min with PBS and added one drop of fluoromount-G mounting medium (SouthernBiotech, Cat. No.: 0100-01) to each well prior to applying a cover slip.
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bioRxiv - Cell Biology 2023Quote: ... 5 min each and mounted in Fluoromount-G (Southern Biotech). Confocal analysis was performed on a Zeiss LSM 780 inverted microscope ...
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bioRxiv - Immunology 2019Quote: ... were coated overnight at 4°C with goat anti-mouse Ig (SouthernBiotech). After blocking with 2% BSA ...
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bioRxiv - Cell Biology 2020Quote: ... coverslips were mounted with 4–6μL of Fluoromount G (SouthernBiotech, 0100-01) on slides (KNITTEL Starfrost ...
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bioRxiv - Biochemistry 2019Quote: ... coverslips were mounted with 4-6 µL Fluoromount G (SouthernBiotech, 0100-01) on slides (KNITTEL Starfrost ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Plates were incubated with serially diluted serum or gut content (50 μL, PBS 1% BSA, wt/vol, 2 h, RT) and standard mouse IgA (Southern Biotech #0106-01, prepared in duplicates) and washed (5x ...
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bioRxiv - Immunology 2020Quote: ... Plates were incubated with serially diluted mouse sera (50 µL, PBS 1% BSA, wt/vol, 2 h, RT) and standard mouse IgG (Southern Biotech #0107-10, prepared in duplicates) and washed (5x ...
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bioRxiv - Microbiology 2021Quote: ... The plates were washed a second time and incubated for 2 h with Streptavidin-alkaline phosphatase from Southern Biotech (2 µg/mL). The final wash was followed by the addition of Nitro-blue Tetrazolium Chloride/5-bromo-4-chloro 3 ‘indolyphosphate p-toludine salt (NBT/BCIP chromagen ...
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bioRxiv - Immunology 2024Quote: ... goat F(ab′)2 anti-human kappa FITC and goat F(ab′)2 anti-human lambda FITC (SouthernBiotech; to detect antibody expression), and PI (Invitrogen ...
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bioRxiv - Immunology 2021Quote: Goat F(ab’)2 anti-mouse Igκ (Southern Biotech) was biotinylated with 20-fold molar excess of NHS-LC-LC-biotin (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... goat F(ab’)2 anti-mouse IgM (αIgM, SouthernBiotech) or rat anti-mouse CD40 (αCD40 ...
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bioRxiv - Developmental Biology 2023Quote: ... post-fixed in 4% paraformaldehyde and coverslipped with DAPI-Fluoromount® G (SouthernBiotech). Third-generation mRNA ISH by chain reaction (HCR ...
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bioRxiv - Developmental Biology 2023Quote: ... post-fixed in 4% paraformaldehyde and coverslipped with DAPI-Fluoromount® G (SouthernBiotech), while wholemounts were rinsed ...
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bioRxiv - Cancer Biology 2020Quote: Primary CLL samples were treated with 20 µg/ml anti-IgM F(ab’)2 or control F(ab’)2 (both Southern Biotech, Cambridge Biosciences, UK) for 5 minutes at 37°C/5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... F(ab’)2 fragments or human IgG1 (control) (Southern Biotech) for 1hr at 37°C in a 96-well plate ...
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bioRxiv - Biochemistry 2020Quote: ... 6-diamidino-2-phenylindole (DAPI; Southern Biotech, Birmingham, AL, USA). Images were made using the Zeiss LSM880 (Oberkochen ...
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bioRxiv - Systems Biology 2023Quote: ... followed by 5 min Hoechst incubation and mounting with Fluoromount (Southern Biotech). Images were taken using an Olympus BX63 microscope and analyzed in ImageJ.
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bioRxiv - Immunology 2023Quote: ... or 5 µg/mL of goat anti-mouse IgG/IgM (Southern Biotech) overnight at 4℃ ...
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bioRxiv - Immunology 2021Quote: ... goat anti-mouse IgM (4 μg/ml; Southern Biotech, Cat#1020-05; RRID: AB_2794201) or goat anti-mouse IgA (4 μg/ml ...
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bioRxiv - Microbiology 2022Quote: ... α-IgA (mAb 11-44-2, FITC, SouthernBiotech catalog no. 1165-02). Cells were stained with primary antibodies (1:50 dilution ...
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bioRxiv - Bioengineering 2019Quote: ... for 2 hr and mounted with Fluoromount-G (SouthernBiotech; 0100-20). Imaging was performed on a Nikon A1Rsi confocal microscope.
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bioRxiv - Immunology 2021Quote: ... or goat anti-mouse IgA (4 μg/ml; Southern Biotech, Cat#1040-05; RRID: AB_2714213). The reaction was visualized by subsequent addition of 2,2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed and incubated at 4°C with goat anti-mouse Ig-PE (SouthernBiotech). Cells were washed and mean fluorescence intensity (MFI ...
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bioRxiv - Pathology 2024Quote: ... or anti-type I collagen at 4 µg/ml (Southern Biotech 1310-01, Birmingham, AL) using heat-induced epitope retrieval at basic pH ...
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bioRxiv - Cancer Biology 2021Quote: ... before counterstaining with DAPI (5 min) and mounting using Fluoromount G (SouthernBiotech, Cambridge, UK). Slides were imaged with an Axioscan.Z1 (Zeiss ...
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bioRxiv - Microbiology 2020Quote: ... followed by goat anti-human kappa-Alexa Fluor 647 (5 μg/ml, Southern biotech) for another 20 minutes at 4°C to allow discrimination between cell adherent (FITC+/Alexa fluor 647+ ...
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bioRxiv - Cell Biology 2021Quote: ... wells were washed then incubated for 5 mins in DAPI Fluoromount G (Southern Biotech) to stain nuclear and kinetoplastid DNA ...
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bioRxiv - Biochemistry 2023Quote: ... in PBS for 5 min and mounted the cells with Fluoromount-G (Southern Biotech). For negative control ...