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Citations for SouthernBiotech :
1301 - 1336 of 1336 citations for Human Anti NT5E Recombinant Antibody clone Hu101 28 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Cells were depleted of B cells and partially depleted of AMs and cells that had lost membrane integrity by first incubating with anti-IgD Biotin (Southern Biotech) and anti-Siglec-F Biotin ...
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bioRxiv - Immunology 2024Quote: ... The secondary antibodies used for western blot analysis were goat anti-rabbit conjugated to horseradish peroxidase-conjugated (HRP) (#4050-05, diluted at 1:1000) and anti-mouse-HRP (#1031-05, diluted at 1:1000) from SouthernBiotech (USA).
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bioRxiv - Microbiology 2023Quote: ... the wells were washed with the washing buffer twice and incubated with horseradish peroxidase (HRP)-conjugated mouse anti-monkey IgG (Southern Biotech) at room temperature for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... and incubated with pleural lavage fluid overnight at 4°C prior to detection of bound IgM with a goat anti-mouse IgM-HRP (1020-05 Southern Biotech) for 1h at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... then washed and secondary antibody added at 1:2000 or 1:4000 dilution in blocking buffer (100□μl/well) using either anti-mouse IgG-HRP (Catalog #1030-05, Southern Biotech), or anti-mouse IgA-biotin (Catalog #1040-80 ...
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bioRxiv - Immunology 2024Quote: ... diluted to 1 μg/ml in T-PBS followed by incubation for 1 hour with goat-anti mouse IgG conjugated with HRP (Southern Biotech) diluted 2000 × in T-PBS.
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bioRxiv - Immunology 2024Quote: ... Specific IgG was detected for 1 hour at room temperature with biotin rat anti-mouse IgG (Southern Biotech, Birmingham, AL, USA) biotin rat anti-mouse IgG1 (Southern Biotech) ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed with ELISA wash buffer then incubated for 1 h at RT with an HRP conjugated goat anti-mouse (Southern Biotech). Plates were washed with ELISA wash buffer and developed using 1-Step Ultra TMB-ELISA substrate solution (Thermo Fisher) ...
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bioRxiv - Immunology 2024Quote: ... diluted to 1 μg/ml in T-PBS followed by incubation for 1 hour with goat-anti mouse IgG conjugated with HRP (Southern Biotech) diluted 2000 × in T-PBS ...
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bioRxiv - Immunology 2024Quote: ... samples were incubated with 100 μl of a 1:500 diluted phycoerythrin (PE)-tagged goat-anti mouse IgG-Fc (Southern Biotech) in the dark at RT with shaking at 600 rpm for 30 min ...
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bioRxiv - Immunology 2022Quote: ... plates were washed 4× with ELISA wash buffer and a 1:4000 dilution of goat anti-mouse IgG (SouthernBiotech, catalog #1030-05) in ELISA blocking buffer was added to each well and incubated at 37°C for 1 hour followed by 4 washes with ELISA wash buffer ...
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bioRxiv - Bioengineering 2022Quote: ... The blots were washed with PBS and further developed with goat anti-rabbit IgG-horseradish peroxidase labeled (HRP) (Southern Biotech #4050-05) diluted 1:1000 for 1 h at RT ...
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bioRxiv - Biochemistry 2022Quote: ... and then treated with alkaline phosphatase (AP)-conjugated goat anti-rabbit-IgG (Southern Biotech; 1:8000 in TBST with 2% (w/v) bovine serum albumin) ...
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bioRxiv - Immunology 2020Quote: ... plates were subsequently incubated with 0.1 µg/mL HRP-labeled goat anti-mouse κ light chain Ab (Southern Biotech, Birmingham, AL 35260), diluted in assay buffer for 1 hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... except constructs were bound directly to the plate and detected with goat anti-mouse IgG HRP conjugate (Southern Biotech, Birmingham, AL, USA).
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bioRxiv - Immunology 2023Quote: ... plates were washed 3 times and incubated with 50 μl of either 0.25 μg/ml biotinylated anti-mouse IgG1 (Southern Biotech, 1070-08), or biotinylated anti-mouse IgG (H+L ...
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bioRxiv - Immunology 2023Quote: ... Plates were then incubated for 90min at RT with goat anti-mouse IgG conjugated to horseradish peroxidase (HRP) (Southern Biotech, #1030-05) at 1:5000 dilution in blocking solution ...
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bioRxiv - Immunology 2023Quote: ... Beads were prepared as described for Eα peptide presentation and coated with different ratios of biotinylated anti-IgM (Southern Biotech, #1021-08) and biotinylated ovalbumin (OVA ...
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bioRxiv - Immunology 2024Quote: ... Plates were then washed 3x with wash buffer and incubated with a 1:4,000 dilution (in EZ block) of Goat Anti-Mouse IgG Fc-HRP secondary (Southern Biotech, 1033-05) for 1 hr at RT ...
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bioRxiv - Immunology 2020Quote: ... followed by two rounds of fluorescence-activated cell sorting (FACS) of cells stained with FITC-Conjugated rat anti-mouse IgG1 (Southern Biotech 1144-02), and rounds of soft-agar cloning in SeaPlaque agarose ...
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bioRxiv - Immunology 2021Quote: ... IgM or IgA were detected by adding HRP-conjugated goat anti-mouse IgG (4 μg/ml; Southern Biotech, Cat#1030-05; RRID: AB_2619742), goat anti-mouse IgM (4 μg/ml ...
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bioRxiv - Microbiology 2021Quote: ... Goat anti-rabbit IgG-HRP (Cat No. 4030-05) and Goat anti-mouse IgG-HRP (Cat No. 1030-05) was from Southern Biotech (Alabama, USA). Goat anti-rabbit IgG Alexa fluor 488 (Cat no ...
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bioRxiv - Microbiology 2022Quote: ... Goat anti-rabbit IgG-HRP (Cat No. 4030-05) and Goat anti-mouse IgG- HRP (Cat No. 1030-05) was from Southern Biotech (Alabama, USA). U0126 (Cat no ...
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bioRxiv - Microbiology 2022Quote: ... PrPSc on membranes was detected using mAb PRC5 at a dilution of 1:5000 followed by alkaline phosphatase conjugated goat anti mouse IgG (Southern Biotech, 1030-04) at a dilution of 1:5000 ...
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bioRxiv - Immunology 2022Quote: ... ELISA plates were coated with 1 μg/ml recombinant SARS-CoV-2 S protein (43) and serially diluted serum samples were added and detected via anti-monkey IgG-HRP (Southern Biotech, Birmingham, AL). Plates were developed using a TMB substrate (source ...
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bioRxiv - Microbiology 2024Quote: ... Plates were washed five times with PBS before adding 100 uL of HRP-conjugated goat anti-mouse IgG1 (Southern Biotech, Birmingham, AL, USA) diluted 1:4000 in blocking buffer and were then incubated overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... primary B cells (107/ml) were stained for 5 min on ice with 10 μg/ml of biotinylated anti-IgM (Southern Biotech, 1021-08) and washed with Imaging Buffer ...
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bioRxiv - Immunology 2023Quote: ... sonicated to disrupt bead:bead aggregates (Bioruptor®: HIGH, 5 cycles, 30” on/off) and coated with different ratios of biotinylated anti-IgM (Southern Biotech, #1021-08) and biotinylated Eα52-68 peptide (Biotin-GSGFAKFASFEAQGALANIAVDKA-COOH ...
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bioRxiv - Immunology 2020Quote: ... Horse spleen ferritin-specific IgA levels in the supernatant of the faecal homogenates was determined by enzyme-linked immunosorbent assay in plates coated with horse spleen ferritin followed by detection with horseradish peroxidase-conjugated goat anti-mouse IgA (Southern Biotech, Birmingham, AL, USA) using optEIA (BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: Primary CLL samples were treated with 20 µg/ml anti-IgM F(ab’)2 or control F(ab’)2 (both Southern Biotech, Cambridge Biosciences, UK) for 5 minutes at 37°C/5% CO2 ...
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bioRxiv - Immunology 2020Quote: ... Plates were then incubated with a 1:1000 dilution of mouse serum raised against ZE3-6H followed by goat anti-mouse IgG HRP conjugate (Southern Biotech, Birmingham, AL, USA) for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Then the cells were stained with DAPI solution and washed three times before mounting with Fluoromount-G® Anti-Fade Mounting Medium (Southern Biotech, Birmingham, AL). Images were captured using Zeiss Observer Z1 confocal microscope (Carl Zeiss ...
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bioRxiv - Immunology 2024Quote: ... 2 x 106 sorted CD4+ T cells and 2 x 106 sorted B cells were co-cultured for 4 – 5 days in the presence of Dynabeads, 10 ng/mL CpG-B (ODN 2006) (Fischer Scientific, Cat#: HC4039) and 10 µg/mL anti-IgM (Southern Biotech, Cat#: 1021-01). For rapamycin pretreatment ...
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bioRxiv - Immunology 2021Quote: ... FO B cells and MZ B cells were cultured in RPMI medium + Pen/Strep + L-glutamine + 10% FBS and stimulated with polyclonal goat anti-mouse IgM (Southern Biotech; 0.6-150 ug/mL), or equimolar concentrations of the Fab’2 fragments (Southern Biotech ...
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bioRxiv - Immunology 2021Quote: To detect mouse Ig Nunc MaxiSorp ELISA plates were coated for 1h at room temperature with goat anti-mouse Ig (H+L) (4 μg/ml; Southern Biotech, Cat#1010-01; RRID: AB_2794121). Citrobacter rodentium-specific Ig was quantified by coating Nunc MaxiSorp ELISA plates with 108 bacteria/well heat-inactivated at 60°C for 1 h ...
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bioRxiv - Immunology 2023Quote: ... Membranes were washed with TBST (1x, 5 min., RT) and incubated with goat anti-rabbit IgG- HRP (Southern Biotech #1021-05, 10 mL, 1:5,000, 1 h, RT). Membranes were washed with TBST (3x ...