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Citations for SouthernBiotech :
1051 - 1100 of 1171 citations for Rat Anti Muellerian hormone type 2 receptor AMHR2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... PVDF membranes containing blotted proteins were washed in excess PBS-Tween 20 for 1h at room temperature before application of a 1:2000 dilution of primary mouse Anti-Human IgA1 antibodies conjugated to biotin (SouthernBiotech). Blots were washed 10 min 3 x in PBS followed by treatment with a 1:2000 dilution of ExtrAvidin®−Peroxidase conjugate (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... A baseline of 30 s was recorded before stimulation with 1 µg/ml anti-λ antibody (Southern Biotech, Birmingham, AL, USA). Flow cytometry standard files were analyzed using FlowjoTM software for Windows ...
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bioRxiv - Immunology 2021Quote: ... washing with wash buffer (1xPBS+0.05% Tween) and incubation with secondary antibody - Mouse-anti-monkey IgG-HRP (Southern Biotech, 4700-05) for 1h ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 4X in wash buffer and the secondary antibody Goat anti-Human Ig-HRP (Southern Biotech, Cat# 2010-05), was added and incubated at 37°C for 1 hr ...
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bioRxiv - Immunology 2021Quote: ... This will be followed by a 1 h incubation with biotinylated HRP conjugated goat anti-mouse IgG subtype antibodies (Southern Biotech). 3,3’,5,5’ tetramethylbenzidine (TMB ...
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bioRxiv - Immunology 2019Quote: ... Sera were incubated 2 h at 37°C on wells pre-coated with 5 μg/mL of NP15-BSA or goat-anti-mouse IgM (Southern Biotech) and saturated with PBS 2% BSA ...
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bioRxiv - Biochemistry 2020Quote: ... which was produced by the conjugation reaction between anti-fluorescein antibody (600101096; Rockland Immunochemicals, PA or 6400-01; Southern Biotech, AL) and NHS-activated Sepharose 4 Fast Flow (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2020Quote: ... and then incubation for 30 mins at room temperature with anti-human IgG HRP at 1:4000 (Southern Biotech #2040-05). 5 washes of TBST were done followed by development using 1-Step Ultra TMB-ELISA Substrate Solution (ThermoFisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... before adding 50 μL/well of a 1:5000 dilution of HRP-conjugated goat anti-mouse IgG (Southern Biotech, #1030-05) in TBSF+MnCl2 ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed with water three times before applying 25 μl of secondary antibody (goat anti-human IgG Fc-Southern Biotech, 2048-04 ...
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bioRxiv - Microbiology 2022Quote: ... the beads were incubated with 1 µg/mL Alexa Fluor647-conjugated goat F(ab’)₂ anti-human kappa (Southern Biotech, 2062-31) in PBS-TH for 30 min at 4 °C with shaking ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed three times and incubated with the goat anti-human-IgG Fc secondary antibody conjugated with alkaline phosphatase (AP, Southern Biotech) at a 1:1000 dilution in blocking buffer for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Plates were coated with 1.25µg/mL soluble RBD (produced previously13) and mouse serum Ig was detected using a goat anti mouse Ig coupled to HRP (Southern Biotech) diluted at 1/6000.
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bioRxiv - Immunology 2020Quote: ... 3 columns on each plate were coated with 1:1000 dilution each of goat anti-mouse Kappa and Lambda light chains (Southern Biotech). After overnight incubation at 4ºC ...
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bioRxiv - Microbiology 2020Quote: ... followed by the addition of horseradish peroxidase-conjugated goat anti-mouse-IgG Fc secondary antibody (1:2000) (Southern Biotech, 1033-05). After incubation for 2 hours at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... in PBST or PBSTF or 1:1000 anti-hamster-IgG2/IgG3-HRP in PBST or PBSTF (Southern Biotech Cat. #1935-05). Plates were incubated at room temperature for 1-2 h ...
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bioRxiv - Systems Biology 2022Quote: ... for capture and biotinylated anti-human insulin antibody (Eli Lilly and Company) with horseradish peroxidase conjugated streptavidin (7100-05, Southern Biotech) for detection ...
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bioRxiv - Immunology 2022Quote: ... The plates were then washed with PBS containing 0.1% Tween-20 (PBS-T), and Alkaline Phosphatase-conjugated Goat Anti-Human IgG (dilution 1:500, catalog no. 2040-04) from Southern Biotech was added and incubated for 45 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Bound serum antibodies were then detected with a goat anti-mouse IgG-HRP secondary antibody (Southern Biotech 1030-05, Birmingham, AL) at 1:10,000 for 1 hour at room temperature ...
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bioRxiv - Immunology 2019Quote: ... washed 6x 300μl PBST and a 1/5000 cocktail of anti-human kappa-HRP and lambda-HRP antibodies (SouthernBiotech, Birmingham, AL) added for 1 hour in assay diluent ...
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bioRxiv - Immunology 2019Quote: ... Plates were washed with PBS/0.1% Tween 20 and wells were incubated with alkaline phosphatase-conjugated anti-human Fc (Southern Biotech; P7998) followed by 2 hour incubation ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed 4X in wash buffer and the secondary antibody Goat anti-Human Ig-HRP (Southern Biotech, Cat# 2010-05), Peroxidase-conjugated AffiniPure Donkey Anti-Human IgG ...
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bioRxiv - Immunology 2019Quote: ... Cells were washed with PBS–0.1% Tween 20 and incubated with mouse anti-human IgG Fc-HRP (Southern Biotech; 9040-05) for 1 hour ...
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bioRxiv - Immunology 2019Quote: ... Plates were washed with PBS–0.1% Tween 20 and incubated with mouse anti-human IgG Fc-HRP (Southern Biotech; 9040-05) for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... plates were washed 4 times with PBS-T and an anti-monkey IgG conjugated to horseradish peroxidase (Southern Biotech 4700-5) 1 hour at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Antibody binding to the spike proteins was determined using a 1:2000 diluted HRP conjugated goat anti-human IgG (ITK Southern Biotech) for 1 h at RT and tetramethylbenzidine substrate (BioFX) ...
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bioRxiv - Microbiology 2020Quote: ... the peptide arrays were incubated with a 1/1000 dilution of an appropriate antibody peroxidase conjugate (goat anti-human HRP conjugate, Southern Biotech, cat ...
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bioRxiv - Immunology 2021Quote: ... followed by washing 3 times with PBST and then a 1:20000 dilution of goat anti-rabbit IgG-HRP (Southern Biotech). After washing 3 times with PBST ...
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bioRxiv - Immunology 2021Quote: ... buffer (PBS with 0.1% bovine serum albumin and 0.1% sodium azide) containing the PE-labeled tetrameric pBF2*1501 complex and fluorescein isothiocyanate (FITC)-labeled anti-CD8 monoclonal antibody (SouthernBiotech, USA) for 30 min at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The following day the plates were washed with PBS-T and incubated with goat anti-mouse IgG1-HRP (Southern Biotech, USA) at 1:6000 dilution in blocking buffer and left for 1 hour at 37°C ...
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bioRxiv - Immunology 2021Quote: ... incubated for 1 hour at room temperature with mouse anti-human IgG conjugated to horse radish peroxidase (Southern Biotech, Birmingham, USA) diluted 1:5000 in blocking solution ...
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bioRxiv - Immunology 2020Quote: ... followed by washing 3 times with PBST and then a 1:20000 dilution of goat anti-rabbit IgG-HRP (Southern Biotech). After washing 3 times with PBST ...
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bioRxiv - Immunology 2020Quote: ... followed by addition of HRP-conjugated goat anti-mouse IgG second antibody (catalog number 1030-05, Southern Biotech, Birmingham, AL, USA). TMB substrate was added and the plates read at OD 450 nm with a plate reader ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse/Human ads-HRP (4050-05, dilution 1:10000) and Goat Anti-Mouse IgG(H+L) Human ads-HRP (1031-05, dilution 1:10000) (Southern Biotech); sheep anti-TGN38 (AHR499G ...
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bioRxiv - Immunology 2022Quote: ... The cells were then stained with 50 μL of the secondary mix (goat anti-human IgG-AF488 (SouthernBiotech, cat. #2040-30) and goat anti-mouse Alexa Fluor 488 IgG (H + L ...
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bioRxiv - Immunology 2022Quote: ... NP-OVA specific IgG1 was detected as previously described48 using plates were coated with TNP(5)-BSA and detected using goat anti-mouse IgG1-HRP (Southern Biotech).
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bioRxiv - Immunology 2022Quote: ... at 37 °C for 1 hr followed by the multi-species SP ads-HRP-conjugated goat anti-mouse IgG (H+L) antiserum (Southern Biotech) diluted at 1:20.000 and incubated at 37 °C for 1 hr ...
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bioRxiv - Immunology 2022Quote: ... The microtiter plates were developed with horseradish peroxidase (HRP)-labeled goat anti-human IgG or IgM polyclonal antibodies (SouthernBiotech, Birmingham, AL). The plates were developed with 3,3 ...
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bioRxiv - Immunology 2022Quote: ... The plates were then washed three times with water and 200 μl of goat anti-human IgG HRP secondary antibody (Southern Biotech) diluted to a ratio of 1:2000 in block buffer was added and incubated for 1 hr at room temperature followed by an hour of incubation ...
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bioRxiv - Immunology 2022Quote: ... Cells were depleted of B cells and partially depleted of AMs and cells that had lost membrane integrity by first incubating with anti-IgD Biotin (Southern Biotech) and anti-Siglec-F Biotin ...
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bioRxiv - Immunology 2023Quote: ... was added to the wells for 1h followed by washing and incubation with horseradish peroxidase-linked goat anti-mouse IgG secondary antibody (Southern Biotech). The avidity index was calculated as ...
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bioRxiv - Cell Biology 2023Quote: ... as well as FITC- and Texas Red-conjugated goat polyclonal anti-human lambda-light chain (cat. 2070-02 and 2070-07) were obtained from Southern Biotech and were used for immunofluorescent microscopy ...
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bioRxiv - Microbiology 2023Quote: ... the wells were washed with the washing buffer twice and incubated with horseradish peroxidase (HRP)-conjugated mouse anti-monkey IgG (Southern Biotech) at room temperature for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... and incubated with pleural lavage fluid overnight at 4°C prior to detection of bound IgM with a goat anti-mouse IgM-HRP (1020-05 Southern Biotech) for 1h at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... then washed and secondary antibody added at 1:2000 or 1:4000 dilution in blocking buffer (100□μl/well) using either anti-mouse IgG-HRP (Catalog #1030-05, Southern Biotech), or anti-mouse IgA-biotin (Catalog #1040-80 ...
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bioRxiv - Cell Biology 2023Quote: ... Surface-bound sLAP was detected with anti-human PE antibodies that bound directly to the Fc-IgG part of the chimeric sLAP proteins (SouthernBiotech, USA). PE signals were analyzed with FlowJo software (BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies conjugated with HRP used in the study are: Goat Anti-mouse IgG1-HRP (1:10000, Southern Biotech, 1071-05), Goat Anti-rabbit IgG-HRP (1:10000 ...
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bioRxiv - Immunology 2022Quote: ... NP-specific IgM and IgG3 antibodies were detected using biotinylated anti-mouse IgM- or IgG3-specific immunoglobulins followed by streptavidin-HRP (Southern Biotech) and developed with SIGMAFAST OPD tablets (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and stained with AF647-conjugated goat anti-human IgG or IgA as appropriate (2050-31 and 2040-31, Southern Biotech) and analyzed by flow cytometry as described above.
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bioRxiv - Immunology 2023Quote: ... bead bound antigen-specific antibodies were detected by using PE-coupled detection antibody (anti-IgG, IgA1, IgM, IgG1, IgG2, IgG3 and IgG4 from Southern Biotech) (1µg/mL) ...