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Citations for SouthernBiotech :
951 - 1000 of 1089 citations for Sheep Anti Chikungunya Virus since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... and then incubation for 30 mins at room temperature with anti-human IgG HRP at 1:4000 (Southern Biotech #2040-05). 5 washes of TBST were done followed by development using 1-Step Ultra TMB-ELISA Substrate Solution (ThermoFisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... before adding 50 μL/well of a 1:5000 dilution of HRP-conjugated goat anti-mouse IgG (Southern Biotech, #1030-05) in TBSF+MnCl2 ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed with water three times before applying 25 μl of secondary antibody (goat anti-human IgG Fc-Southern Biotech, 2048-04 ...
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bioRxiv - Microbiology 2022Quote: ... the beads were incubated with 1 µg/mL Alexa Fluor647-conjugated goat F(ab’)₂ anti-human kappa (Southern Biotech, 2062-31) in PBS-TH for 30 min at 4 °C with shaking ...
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bioRxiv - Immunology 2022Quote: ... IgG bound to the rSARS-CoV-2 S protein was detected using a horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody (Southern Biotech) incubated for 1 hour ± 10 minutes at 24°C ± 2°C ...
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bioRxiv - Immunology 2022Quote: ... a standard curve for mouse isotype was established for each plate by coating 2 columns with 50 μL goat anti-mouse Ig (1 μg/mL in PBS; Southern Biotech) overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed three times and incubated with the goat anti-human-IgG Fc secondary antibody conjugated with alkaline phosphatase (AP, Southern Biotech) at a 1:1000 dilution in blocking buffer for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Plates were coated with 1.25µg/mL soluble RBD (produced previously13) and mouse serum Ig was detected using a goat anti mouse Ig coupled to HRP (Southern Biotech) diluted at 1/6000.
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bioRxiv - Immunology 2020Quote: ... 3 columns on each plate were coated with 1:1000 dilution each of goat anti-mouse Kappa and Lambda light chains (Southern Biotech). After overnight incubation at 4ºC ...
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bioRxiv - Microbiology 2020Quote: ... followed by the addition of horseradish peroxidase-conjugated goat anti-mouse-IgG Fc secondary antibody (1:2000) (Southern Biotech, 1033-05). After incubation for 2 hours at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... in PBST or PBSTF or 1:1000 anti-hamster-IgG2/IgG3-HRP in PBST or PBSTF (Southern Biotech Cat. #1935-05). Plates were incubated at room temperature for 1-2 h ...
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bioRxiv - Microbiology 2022Quote: ... bacteria were incubated with a Goat F(ab’)2 anti-human Kappa-Alexa Fluor 647 (pre-diluted in PBS 0.1%BSA; 2.5 μg/mL, Southern Biotech #2062-31) to detect bound Fab fragments ...
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bioRxiv - Systems Biology 2022Quote: ... for capture and biotinylated anti-human insulin antibody (Eli Lilly and Company) with horseradish peroxidase conjugated streptavidin (7100-05, Southern Biotech) for detection ...
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bioRxiv - Immunology 2022Quote: ... the hACE2 receptor bound to the S protein was then detected using a mouse anti-His-Tag horseradish peroxidase conjugate (Southern Biotech) and a colorimetric signal generated by the addition of 3,3′,5,5′-TMB substrate ...
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bioRxiv - Immunology 2022Quote: ... The plates were then washed with PBS containing 0.1% Tween-20 (PBS-T), and Alkaline Phosphatase-conjugated Goat Anti-Human IgG (dilution 1:500, catalog no. 2040-04) from Southern Biotech was added and incubated for 45 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Bound serum antibodies were then detected with a goat anti-mouse IgG-HRP secondary antibody (Southern Biotech 1030-05, Birmingham, AL) at 1:10,000 for 1 hour at room temperature ...
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bioRxiv - Immunology 2019Quote: ... washed 6x 300μl PBST and a 1/5000 cocktail of anti-human kappa-HRP and lambda-HRP antibodies (SouthernBiotech, Birmingham, AL) added for 1 hour in assay diluent ...
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bioRxiv - Immunology 2019Quote: ... Plates were washed with PBS/0.1% Tween 20 and wells were incubated with alkaline phosphatase-conjugated anti-human Fc (Southern Biotech; P7998) followed by 2 hour incubation ...
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bioRxiv - Immunology 2019Quote: ... the membrane was re-blocked with 5% BSA in 1x TBST for 2 hours at room temperature before probing with 1:5000 dilution of anti-human IgG-HRP (Southern Biotech) in 5% BSA/1x TBST for 1 hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... or IgG purified from Ggta1+/+ or Ggta1-/- mouse serum (60 µg/mL, PBS 2% BSA, wt/vol, 30 min.) followed by anti-mouse IgG-PE (Southern Biotech #1030-09 ...
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bioRxiv - Immunology 2020Quote: ... for detection of αGal and IgG purified from Ggta1+/+ or Ggta1-/- mouse serum (60 µg/mL, PBS 2% BSA, wt/vol, 30 min.) followed by anti-mouse IgG-PE (Southern Biotech #1030-09 ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed 4X in wash buffer and the secondary antibody Goat anti-Human Ig-HRP (Southern Biotech, Cat# 2010-05), Peroxidase-conjugated AffiniPure Donkey Anti-Human IgG ...
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bioRxiv - Immunology 2019Quote: ... Cells were washed with PBS–0.1% Tween 20 and incubated with mouse anti-human IgG Fc-HRP (Southern Biotech; 9040-05) for 1 hour ...
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bioRxiv - Immunology 2019Quote: ... Plates were washed with PBS–0.1% Tween 20 and incubated with mouse anti-human IgG Fc-HRP (Southern Biotech; 9040-05) for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... plates were washed 4 times with PBS-T and an anti-monkey IgG conjugated to horseradish peroxidase (Southern Biotech 4700-5) 1 hour at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Antibody binding to the spike proteins was determined using a 1:2000 diluted HRP conjugated goat anti-human IgG (ITK Southern Biotech) for 1 h at RT and tetramethylbenzidine substrate (BioFX) ...
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bioRxiv - Microbiology 2020Quote: ... the peptide arrays were incubated with a 1/1000 dilution of an appropriate antibody peroxidase conjugate (goat anti-human HRP conjugate, Southern Biotech, cat ...
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bioRxiv - Microbiology 2021Quote: ... Fab fragments were detected with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech, 1:500). After washing ...
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bioRxiv - Bioengineering 2021Quote: ... arrays were incubated for an additional 2 h at RT and 60 rpm with goat anti-mouse IgG conjugated to HRP (Southern Biotech) diluted 1:5000 in blocking solution ...
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bioRxiv - Immunology 2021Quote: ... followed by washing 3 times with PBST and then a 1:20000 dilution of goat anti-rabbit IgG-HRP (Southern Biotech). After washing 3 times with PBST ...
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bioRxiv - Immunology 2021Quote: ... buffer (PBS with 0.1% bovine serum albumin and 0.1% sodium azide) containing the PE-labeled tetrameric pBF2*1501 complex and fluorescein isothiocyanate (FITC)-labeled anti-CD8 monoclonal antibody (SouthernBiotech, USA) for 30 min at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The following day the plates were washed with PBS-T and incubated with goat anti-mouse IgG1-HRP (Southern Biotech, USA) at 1:6000 dilution in blocking buffer and left for 1 hour at 37°C ...
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bioRxiv - Immunology 2021Quote: ... incubated for 1 hour at room temperature with mouse anti-human IgG conjugated to horse radish peroxidase (Southern Biotech, Birmingham, USA) diluted 1:5000 in blocking solution ...
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bioRxiv - Immunology 2020Quote: ... followed by washing 3 times with PBST and then a 1:20000 dilution of goat anti-rabbit IgG-HRP (Southern Biotech). After washing 3 times with PBST ...
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bioRxiv - Immunology 2020Quote: ... followed by addition of HRP-conjugated goat anti-mouse IgG second antibody (catalog number 1030-05, Southern Biotech, Birmingham, AL, USA). TMB substrate was added and the plates read at OD 450 nm with a plate reader ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse/Human ads-HRP (4050-05, dilution 1:10000) and Goat Anti-Mouse IgG(H+L) Human ads-HRP (1031-05, dilution 1:10000) (Southern Biotech); sheep anti-TGN38 (AHR499G ...
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bioRxiv - Biochemistry 2020Quote: ... The immunoreactivity was detected by incubation with Rabbit F(ab’)2 Anti-Goat IgG(H+L)-HRP (6020-05, Southern Biotech) diluted 3000 × in PBS-T.
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bioRxiv - Immunology 2022Quote: ... Hp-specific IgG1 was detected as previously described47 using plates coated with Hp extract and detected using rat anti-mouse IgG1-HRP antibody (Southern Biotech). NP-OVA specific IgG1 was detected as previously described48 using plates were coated with TNP(5)-BSA and detected using goat anti-mouse IgG1-HRP (Southern Biotech).
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bioRxiv - Immunology 2022Quote: ... The cells were then stained with 50 μL of the secondary mix (goat anti-human IgG-AF488 (SouthernBiotech, cat. #2040-30) and goat anti-mouse Alexa Fluor 488 IgG (H + L ...
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bioRxiv - Immunology 2022Quote: ... NP-OVA specific IgG1 was detected as previously described48 using plates were coated with TNP(5)-BSA and detected using goat anti-mouse IgG1-HRP (Southern Biotech).
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bioRxiv - Immunology 2022Quote: ... at 37 °C for 1 hr followed by the multi-species SP ads-HRP-conjugated goat anti-mouse IgG (H+L) antiserum (Southern Biotech) diluted at 1:20.000 and incubated at 37 °C for 1 hr ...
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bioRxiv - Immunology 2022Quote: ... The microtiter plates were developed with horseradish peroxidase (HRP)-labeled goat anti-human IgG or IgM polyclonal antibodies (SouthernBiotech, Birmingham, AL). The plates were developed with 3,3 ...
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bioRxiv - Immunology 2022Quote: ... The plates were then washed three times with water and 200 μl of goat anti-human IgG HRP secondary antibody (Southern Biotech) diluted to a ratio of 1:2000 in block buffer was added and incubated for 1 hr at room temperature followed by an hour of incubation ...
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bioRxiv - Immunology 2022Quote: ... Cells were depleted of B cells and partially depleted of AMs and cells that had lost membrane integrity by first incubating with anti-IgD Biotin (Southern Biotech) and anti-Siglec-F Biotin ...
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bioRxiv - Cell Biology 2023Quote: ... as well as FITC- and Texas Red-conjugated goat polyclonal anti-human lambda-light chain (cat. 2070-02 and 2070-07) were obtained from Southern Biotech and were used for immunofluorescent microscopy ...
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bioRxiv - Immunology 2023Quote: ... and incubated with pleural lavage fluid overnight at 4°C prior to detection of bound IgM with a goat anti-mouse IgM-HRP (1020-05 Southern Biotech) for 1h at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... the wells were washed with the washing buffer twice and incubated with horseradish peroxidase (HRP)-conjugated mouse anti-monkey IgG (Southern Biotech) at room temperature for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... was added to the wells for 1h followed by washing and incubation with horseradish peroxidase-linked goat anti-mouse IgG secondary antibody (Southern Biotech). The avidity index was calculated as ...
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bioRxiv - Biochemistry 2023Quote: ... then washed and secondary antibody added at 1:2000 or 1:4000 dilution in blocking buffer (100□μl/well) using either anti-mouse IgG-HRP (Catalog #1030-05, Southern Biotech), or anti-mouse IgA-biotin (Catalog #1040-80 ...
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bioRxiv - Cell Biology 2023Quote: ... Surface-bound sLAP was detected with anti-human PE antibodies that bound directly to the Fc-IgG part of the chimeric sLAP proteins (SouthernBiotech, USA). PE signals were analyzed with FlowJo software (BD Biosciences ...