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Citations for SouthernBiotech :
951 - 1000 of 1279 citations for Anti MICU2 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... anti-human IgA2 Alexa Fluor 488 (clone: A9604D2, Southern Biotech Cat. No. 9140-30). The samples were incubated for 30 minutes at 4 ° C and directly topped up with 1 mL of a 5 μM Hoechst 33342 solution (Thermo Fischer Scientific Cat ...
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bioRxiv - Microbiology 2020Quote: ... followed by goat anti-human kappa-Alexa Fluor 647 (5 μg/ml, Southern biotech) for another 20 minutes at 4°C to allow discrimination between cell adherent (FITC+/Alexa fluor 647+ ...
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bioRxiv - Neuroscience 2021Quote: ... We then incubated cells with goat-anti-rabbit-APC (Southern Biotech, Cat# 4050-11S), mouse anti-human monoclonal antibodies against p75/NGFR (Becton-Dickinson ...
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bioRxiv - Immunology 2020Quote: ... IgG was detected using HRP-conjugated goat anti-mouse IgG2b (Southern Biotech #1090-05) in PBS/1%BSA (100 µL ...
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bioRxiv - Immunology 2020Quote: ... IgG was detected using HRP-conjugated goat anti-mouse IgG (Southern Biotech #1030-05) in PBS/1%BSA/0.01% Tween-20 (100 µL ...
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bioRxiv - Microbiology 2021Quote: ... and mouse anti-chicken monocyte/macrophage-PE (Clone KUL01, Southern Biotech, Birmingham, AL, USA) antibodies in the dark for 20 min at RT ...
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bioRxiv - Immunology 2020Quote: ... a 1:50,000 dilution of biotinylated mouse anti-monkey IgG was used (Southern Biotech), followed by a 1 h incubation with a 1:3000 dilution of HRP-labeled streptavidin (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... Bound mouse IgG was detected with HRP-conjugated goat anti-mouse IgG (Southern Biotech), and the total IgG concentration was calculated with mouse IgG standards (Jackson ...
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bioRxiv - Immunology 2019Quote: ... Staining was detected using goat anti-mouse IgG FITC at 1:500 (Southern Biotech) ANAs were scored on intensity of nuclear and cytoplasmic staining on a scale of 0–3 and for the presence or absence of mitotic chromatin using wide-field fluorescence microscopy (Olympus IX83 ...
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bioRxiv - Immunology 2021Quote: ... goat anti-mouse IgM (4 μg/ml; Southern Biotech, Cat#1020-05; RRID: AB_2794201) or goat anti-mouse IgA (4 μg/ml ...
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bioRxiv - Immunology 2021Quote: Goat anti-human kappa cross-absorbed against mouse IgG (Southern Biotech catalog # 2061-01) and goat anti-human lambda cross-absorbed against mouse IgG (Southern Biotech catalog # 2071-01 ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight goat anti-mouse IgM (1mg/ml, Southern Biotech, cat# 1021-01) and blocked with coating buffer containing with 1% BSA (w/v ...
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bioRxiv - Genomics 2022Quote: ... and incubated overnight with 1) goat anti-collagen I (1:200; SouthernBiotech; #1310-01) and rabbit anti-MFAP4 (1:100 ...
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bioRxiv - Immunology 2022Quote: ... and horseradish peroxidase-conjugated Goat Anti-Human IgG (Southern Biotech, Cat# 2040-05, RRID:AB_2795644) were used to detect binding ...
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bioRxiv - Immunology 2022Quote: ... followed by incubation with goat anti-mouse IgG1- or IgG2a-adsorbed HRP (Southern Biotech) for 1 hour ...
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bioRxiv - Immunology 2024Quote: ... and Goat anti-Mouse IgG Fc Human ads-PE (Southern Biotech, catalog:1030-09) were added to the beads (6 μg/ml in block buffer ...
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bioRxiv - Immunology 2023Quote: ... plates were washed with wash buffer and anti-monkey IgG HRP (SouthernBiotech, Birmingham AL) was diluted 1:10,000 in blocking buffer and added to each well ...
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bioRxiv - Microbiology 2022Quote: ... horseradish peroxidase (HRP)-conjugated goat anti-cat IgG diluted 1:4,000 (SouthernBioTech, Birmingham, AL) in PBST was added and incubated at RT for 2 h ...
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bioRxiv - Molecular Biology 2023Quote: ... washed in PBS and mounted in anti-fade mounting medium Fluoromount-G (Southern Biotech).
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bioRxiv - Microbiology 2023Quote: ... or goat F(ab’)2 anti-human IgM-PE (5 µg/ml, Southern Biotech) for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... and goat anti-mouse IgG conjugated to HRP (Southern Biotech, 1031-05; 1:10,000). The membrane was again washed three times with TBST and the protein of interest was detected using a chemiluminescence reagent (ECL Prime ...
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bioRxiv - Immunology 2023Quote: ... and 100 µL of anti-human IgG-Fc HRP (clone JDC-10, Southern Biotech), diluted 1:2000 in blocking buffer ...
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bioRxiv - Immunology 2023Quote: ... and ELISAs were detected with HRP-conjugated goat-anti-mouse IgG1-HRP (Southern Biotech). To develop the enzymatic reaction TMB was used as described above ...
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bioRxiv - Immunology 2021Quote: ... and antigen-bound antibodies were detected by using a PE-coupled detection antibody for each subclass and isotype (IgG1, IgG3, IgA1, and IgM; Southern Biotech), and Fcγ-receptors were fluorescently labeled with PE before addition to immune complexes (FcγR2a ...
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bioRxiv - Immunology 2019Quote: ... The arrays were washed 3x 300 μL/well on the plate washer before adding 100 μL/well of a 1/5000 cocktail of biotinylated secondary polyclonal goat antibodies (Southern Biotech) directed to the human kappa and lambda light chains in assay diluent ...
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bioRxiv - Neuroscience 2020Quote: ... slides were incubated at room temperature for 2 hours in secondary antibodies diluted in the blocking solution (at 1:500 for Alexa-coupled antibodies (Life Sciences)) and then mounted using Hoechst-containing Fluoromount-G (Southern Biotech).
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bioRxiv - Cell Biology 2021Quote: ... Samples were rinsed three times with PBS between and after antibody incubations and mounted onto slides using Flouromount-G (Southern Biotech).
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bioRxiv - Immunology 2022Quote: ... and antigen-bound antibodies were detected by using a PE-coupled detection antibody for each subclass and isotype (IgG1, IgG2, IgG3, IgG4, IgA1, and IgM; Southern Biotech), and Fcγ-receptors were fluorescently labeled with PE before addition to immune complexes (FcγR2a ...
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bioRxiv - Microbiology 2021Quote: ... Isotype analysis was perfomed by incubating the immune complexes with secondary goat anti-mouse-PE antibody (IgG1 1070-09, IgG2a 1080-09S, IgG2b 1090-09S, IgG3 1100-09, IgM 1020-09, IgA 1040-09 Southern Biotech) for each isotype ...
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bioRxiv - Immunology 2021Quote: ... and antigen-bound antibodies were detected by using a PE-coupled detection antibody for each subclass and isotype (IgG1, IgG2, IgG3, IgG4, IgA1, and IgM; Southern Biotech), and Fcγ-receptors were fluorescently labeled with PE before addition to immune complexes (FcγR2A ...
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bioRxiv - Cell Biology 2020Quote: ... localization of antibody labeling was detected after a 1 hr incubation with secondary antibody diluted in PBS containing 2% normal goat serum and mounting under a coverslip with DAPI Fluoromount-G (Southern Biotech). PPCs were visualized by fluorescent microscopy and their numbers were assessed qualitatively by visual examination ...
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bioRxiv - Neuroscience 2020Quote: ... After thorough washing fluorescent secondary antibodies were applied at room temperature for 3 hours before counterstaining with DAPI and mounting with FluoroMount G (Southern Biotech). Slides were imaged using a Leica SP8 using 20x (NA 0.75 ...
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bioRxiv - Microbiology 2023Quote: ... Alexa Fluor® Plus 488 secondary antibodies were diluted 1:1,000 in the respective blocking solutions before mounting with 5 µL DAPI Fluoromount-G® (Southern Biotech) and imaging ...
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bioRxiv - Immunology 2023Quote: ... Sections were washed in PBS before incubation with secondary antibodies or labelled streptavidin (Supplementary Table 4) for 1hr at room temperature followed by mounting with DAPI containing fluoromount (Southern Biotech). Small to medium sized airways in lung sections were captured by an investigator blind to sample identity with an EVOS FL imaging system (Thermo) ...
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bioRxiv - Immunology 2023Quote: ... and antigen-bound antibodies were detected by using a PE-coupled detection antibody for each subclass and isotype (IgG1, IgG2, IgG3, IgA1, IgA2 and IgM; Southern Biotech), and Fcγ-receptors were fluorescently labeled with PE before addition to immune complexes (FcγR2A ...
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bioRxiv - Microbiology 2024Quote: ... Concentrations were evaluated by linear regression analysis built by potting OD405 values against a standard curve generated by titration of a human IgG-unlabeled antibody (Southern Biotech).
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bioRxiv - Immunology 2021Quote: ... As positive control cells were stimulated with anti-mouse CD3 [0,05 μg/ml] (Southern Biotech) and anti-mouse CD28 antibody [0,5 μg/ml] (Southern Biotech) ...
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bioRxiv - Immunology 2021Quote: ... washed and detected with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (SouthernBiotech, Birmingham, AL). The ELISA plates were developed using SureBlue 3,3′,5,5′-tetramethylbenzidine (TMB ...
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bioRxiv - Immunology 2020Quote: ... at 1:20,000 dilution or HRP-conjugated polyclonal goat anti-chicken IgA (SouthernBiotech, Birmingham, AL) at 1:10,000 was used as a secondary antibody and plates were incubated for 1 h ...
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FOXO dictate initiation of B cell development and myeloid restriction in common lymphoid progenitorsbioRxiv - Immunology 2022Quote: ... were coated with 10-20µg/ml of anti-mouse immunoglobulin (Ig) (H+L) (Southern Biotech) to capture total Ig in the serum ...
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bioRxiv - Immunology 2019Quote: ... Plates were then washed and incubated with peroxidase-conjugated goat anti-mouse IgM (Southern Biotech). Spots were revealed with AEC (Sigma-Aldrich) ...
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bioRxiv - Immunology 2019Quote: ... Plates were then washed and incubated with peroxidase-conjugated goat anti-mouse IgM (Southern Biotech). Detection was done with TMB Substrate Reagent Set (BD OptEIA ...
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bioRxiv - Immunology 2021Quote: ... and then incubated 90 min with alkaline phosphatase (AP) conjugated anti-human IgG (Southern biotech) diluted at 1:1000 in PBS containing 1% BSA and 0.05% Tween20 ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed and goat anti-mouse IgG Fc-HRP (Southern Biotech, 1013-05) was added to the plate at 1:2000 in PBS ...
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bioRxiv - Systems Biology 2020Quote: ... HRP-conjugated antimouse-IgG (ICN Pharmaceuticals; 55564) or anti-rabbit-IgG (Southern Biotech; 4050-05) were used as secondary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... which were then mounted on slides with Fluoromount-G anti-fade medium without DAPI (SouthernBiotech). For analysis ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed then coincubated with PE-conjugated mouse anti-human IgG Fc (Southern Biotech) diluted to 1:200 for 30 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... 100 µL of goat anti-mouse IgG -HRP (1:4,000, Southern Biotech, Birmingham, AL, USA) or goat anti-hamster IgG-HRP (1:32,000 ...
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bioRxiv - Microbiology 2022Quote: ... or mouse anti-hamster-IgG1-HRP (Southern Biotech, Cat# 1940–05, 1:8000 in PBST) for 2 h at RT ...
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bioRxiv - Bioengineering 2022Quote: ... We added horseradish peroxidase (HRP)-conjugated goat anti-human IgG (1:5000, SouthernBiotech #2016-05) to detect the binding in COVID-19 patients’ serum samples ...