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Citations for SouthernBiotech :
51 - 100 of 850 citations for Vitamin K epoxide reductase complex subunit 1 VKORC1 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The primary antibodies were detected by secondary antibody anti-mouse or anti-rabbit conjugated to horseradish peroxidase (Southern Biotech, 1:10,000 dilution) for 1 hour at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed 3X with PBS and 3X with PBS-T and then anti-mouse IgG/IgA-biotin (Southern Biotech) diluted 1:1000 in PBS-T was added and incubated overnight at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... followed by probing the bound antibodies with an anti-monkey IgG horseradish peroxidase (HRP)-conjugated secondary antibody at 1:4000 dilution (Southern Biotech, Birmingham, AL) for 1hr at RT ...
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bioRxiv - Microbiology 2020Quote: ... Cell lysates and supernatants were subjected to immunoblot analysis for antibodies against cellular LAMP-1 (1:2000, SouthernBiotech, 9835-01), VSV-G (1:2000 ...
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bioRxiv - Bioengineering 2021Quote: ... and then for 1 hour at room temperature with a 1:4000 diluted rabbit anti-mouse HRP secondary antibody (SouthernBiotech Cat# 6170-05 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with FACs buffer (1% BSA, 1% Na+azide) and secondary antibody IgG-PE (Southern Biotech, Alabama, USA) was added in a 1:200 dilution ...
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bioRxiv - Microbiology 2023Quote: ... formaldehyde fixed parasites were adhered to glass slides and blocked with 50% FCS in PBS for 45 min before staining with primary anti-VSG (1:10,000) and secondary Alexa Fluor (1:1,000) antibodies and mounting with DAPI Fluoromount-G® (Southern Biotech). Imaging was performed with an Axioscope 2 fluorescence microscope (Zeiss ...
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bioRxiv - Microbiology 2023Quote: ... formaldehyde fixed parasites were adhered to glass slides and blocked with 50% FCS in PBS for 45 min before staining with primary anti-VSG (1:10,000) and secondary Alexa Fluor (1:1,000) antibodies and mounting with DAPI Fluoromount-G® (Southern Biotech). Imaging was performed with an Axioscope 2 widefield fluorescence microscope (Zeiss ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the cells were incubated with AF488 labeled secondary antibody (SouthernBiotech, 4050-30, 1:500), followed by incubation with DAPI (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... The HRP–conjugated secondary antibodies were purchased from SouthernBiotech (mouse, WB 1:20 000) and Protein A HRP–conjugated were purchased from Invitrogen (WB 1 ...
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bioRxiv - Microbiology 2022Quote: ... The secondary antibody Goat anti-rabbit IgG-HRP (1:5000) (Southern Biotech #4030–05) was diluted in blocking buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Secondary antibodies used are goat anti-rabbit HRP-conjugated (1:5,000, SouthernBiotech, 4010-05) and goat anti-mouse HRP-conjugated (1:5,000 ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... slides were then incubated with biotinylated secondary antibodies (Southern Biotech, Birmingham, AL; 1:200), and the markers were visualized with the avidin-biotin complex (ABC ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated in Goat-Anti-Mouse HRP-conjugated secondary antibody (SouthernBiotech; 1:2000) for 1 h at room temperature and detected by ECL (PerkinElmer) ...
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bioRxiv - Immunology 2023Quote: ... plates were coated with capture antibody (goat anti-mouse IgG1, 1 μg/ml, SouthernBiotech) or cetuximab (20 µg/ml ...
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bioRxiv - Immunology 2022Quote: ... Cells were depleted of B cells and partially depleted of AMs and cells that had lost membrane integrity by first incubating with anti-IgD Biotin (Southern Biotech) and anti-Siglec-F Biotin ...
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bioRxiv - Immunology 2023Quote: ... Bound IL-21 was subsequently detected with mouse anti-human IL-21-biotin (clone 2B2-G20, eBioscience) followed by alkaline phosphatase-conjugated streptavidin (Southern Biotech). The signals were compared to a standard curve generated with recombinant human IL-21 (R&D Systems).
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bioRxiv - Immunology 2024Quote: ... AP-conjugated goat anti-mouse immunoglobulin antibodies were added to detect IgM (1 in 2000) or IgG (1 in 1000) (Southern Biotech). Signal was detected with Sigma FAST P-nitrophenyl phosphate (pNPP ...
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bioRxiv - Immunology 2024Quote: ... Horseradish peroxidase-labelled goat anti-human IgG (IgG-HRP, diluted 1:5000) or IgA (IgA-HRP, diluted 1:2000) secondary antibodies (Southern Biotech) were added at 50 µL/well for one hour at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... or a 1:1 mixture of anti-mouse kappa and anti-mouse lambda capture antibodies at 5 µg/mL (for standards; Southern Biotech) in PBS at 4 °C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... The secondary antibodies used were the following: goat α-mouse-HRP (Southern Biotech, 1:5000) and goat α-rabbit-HRP (Southern Biotech ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by incubation with secondary antibody (anti-rat IgG HRP-conjugated, Southern Biotech, 1:10,000) for 1 h at room temperature ...
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bioRxiv - Genetics 2020Quote: ... followed by incubation with Alexa-488 anti-rabbit secondary antibody (1:1000, #6441-30 SouthernBiotech) for 2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Fibrotic area was detected using anti-Collagen I antibody (1310-01, Southern Biotech, 1:500). CSA and fluorescence intensity were quantified using ImageJ software for statistical analyses.
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bioRxiv - Immunology 2024Quote: ... followed by HRP-conjugated goat anti-rabbit secondary antibody (SouthernBiotech; 1:2,000 in Wash Buffer). Plates were washed and 100 μL TMB was added for 5-10 min followed by 100 µL Stop Solution ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies used were: Goat Anti-rabbit IgG-HRP (1:10000, Southern Biotech, 4030-05) and Goat Anti-mouse IgG2b-HRP (1:10000 ...
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bioRxiv - Biochemistry 2024Quote: ... and an HRP-conjugated rabbit anti-goat antibody (Southern Biotech, 6160-05, 1:5000 dilution). Membranes were washed three times for 5-minutes each using PBST between all the described steps.
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bioRxiv - Microbiology 2021Quote: ... samples were further statically incubated for 1 h at 4°C with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech, 1:300) and 6 μM Syto9 (Live/Dead BacLight Bacterial Viability Kit ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The secondary antibodies were goat anti-rabbit (#4010-05, 1:5000) and anti-mouse (#1030-05, 1:5000, both from Southern Biotech, Birmingham AL) coupled to horseradish peroxidase ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were transferred to nitrocellulose or PVDF membrane and probed with rat anti-FLAG (clone 9H1, produced in-house; 1:1000) primary antibody and anti-rat HRP (Southern Biotech; 1:5000) secondary.
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were detected by incubating membranes with 1:4000 dilution of HRP-conjugated (Southern Biotech) or 1:5000 dilution of IRDye-conjugated (LI-COR ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were detected by incubating membranes with 1:5000 dilution of HRP-conjugated (Southern Biotech) secondary anti-mouse and anti-rabbit antibodies for 1 h at room temperature ...
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bioRxiv - Immunology 2020Quote: ... the plates were washed and developed with streptavidin-HRP antibody (1:1000; SouthernBiotech, Birmingham, AL, USA) and 3,3′,5,5′-tetramethylbenzidine (TMB ...
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bioRxiv - Microbiology 2020Quote: ... followed by addition of rabbit anti-human HRP secondary antibody diluted to 1:5000 (Southern Biotech). Primary and secondary antibodies were incubated in the presence of 3% BSA blocking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... incubated with primary antibody overnight and then anti-mouse IgG1 (Southern Biotech 1071-08, 1:1000) in 20% Superblock (Fisher PI-37535 ...
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bioRxiv - Physiology 2023Quote: ... The membranes were then washed 3x in TBST before incubation for 1 hour at room temperature with peroxidase-conjugated secondary antibodies in TBST at 1:10,000 (Southern Biotech, #1031-05, #4050-05). Antibody binding was detected using an enhanced chemiluminescence HRP substrate detection kit (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... and then incubated with 1:100000 secondary antibody Anti-Mouse Kappa conjugated to Horseradish Peroxidase (Southern Biotech) for 2 h before washing again ...
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bioRxiv - Cell Biology 2020Quote: ... secondary antibodies were incubated for 1 hour at room temperature before mounting in Fluoromount-G medium (SouthernBiotech) for immunochemistry ...
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bioRxiv - Bioengineering 2021Quote: ... and a secondary antibody of goat anti-rabbit IgG-HRP (1:3,000 dilution) (Southern Biotech, Birmingham, AL) for detection of VIN CP-FcAL ...
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bioRxiv - Neuroscience 2021Quote: ... They were then incubated with secondary antibodies (Table 1) and mounted in Fluoromount (SouthernBiotech, Birmingham, AL, USA) for observation.
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bioRxiv - Cell Biology 2022Quote: ... Antibody binding was visualized with peroxidase-conjugated sheep anti-rabbit immunoglobulin (Southern Biotech; 4010-05; 1:15000), or sheep anti-mouse immunoglobulin (GE Healthcare ...
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bioRxiv - Plant Biology 2024Quote: ... Washed membranes were incubated in a 1:6000 dilution of goat anti-rabbit secondary antibody (Southern Biotech) conjugated to alkaline phosphatase in 3% (w/v ...
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bioRxiv - Microbiology 2024Quote: ... The following secondary antibodies were used: rabbit-anti mouse IgG-horseradish peroxidase (HRP; SouthernBiotech; dilution 1:10‘000) and goat-anti rabbit IgG-HRP (SouthernBiotech ...
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bioRxiv - Cell Biology 2022Quote: ... Antibody binding was visualized with peroxidase-conjugated sheep anti-rabbit immunoglobulin (Southern Biotech; 4010-05; 1:15000), sheep anti-mouse immunoglobulin (GE Healthcare ...
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bioRxiv - Plant Biology 2024Quote: ... washed membranes were incubated in a 1:6000 dilution of goat anti-rabbit secondary antibody (Southern Biotech) conjugated to alkaline phosphatase in 3% (w/v ...
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bioRxiv - Microbiology 2024Quote: ... Cells were incubated cell side down in 100 µL of Stellaris RNA FISH Hybridization Buffer (BioSearch) containing M1 vRNA probes (1/100) and the primary antibodies of interest (NP, Southern Biotech, 10770-01, 1/100) and incubated in a humid chamber at 37°C in the dark for 16 h ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated at RT for 1 h with the following HRP-conjugated secondary antibodies: goat anti-rabbit IgG conjugated to HRP (Southern Biotech, 4010-05; 1:5000), and goat anti-mouse IgG conjugated to HRP (Southern Biotech ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies used include the following: Goat Anti-Mouse IgG (H+L; Southern Biotech, 1031-05, 1:2,000) and Goat Anti-Rabbit IgG (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... incubated with a horseradish peroxidase-conjugated goat anti-mouse IgG secondary antibody (SouthernBiotech, 1030-05, 1:2000 dilution) at RT for 1 h ...
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bioRxiv - Immunology 2021Quote: ... 25 μl of 1:5000 diluted horse radish peroxidase (HRP) conjugated anti-Human IgG secondary antibody (Southern Biotech) was added and incubated for 1h at RT ...