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Citations for SouthernBiotech :
51 - 96 of 96 citations for FITC labeled Fibrinogen since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Sections were washed and stained with anti-IgA FITC (clone 11-44-2, Southern Biotech, 1:100) in blocking buffer in the dark for 30min at RT ...
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bioRxiv - Immunology 2021Quote: ... were determined via detection by alkaline phosphatase (AP)-labeled anti-IgM/anti-IgG (SouthernBiotech) by using p-nitro-phenylphosphate (pNPP ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed and HRP-labeled goat anti-mouse IgG (SouthernBiotech, Birmingham, AL) was added ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the cells were incubated with AF488 labeled secondary antibody (SouthernBiotech, 4050-30, 1:500), followed by incubation with DAPI (Roche ...
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bioRxiv - Immunology 2024Quote: ... Slides were washed and stained with Alexa-488 labeled anti-human IgG (Southern Biotech) at rt using supplied buffers ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed with PBS and incubated for 1 h with a secondary goat anti-mouse FITC (SouthernBiotech) at a 20 μg/mL concentration and phalloidin (7 units ...
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bioRxiv - Immunology 2022Quote: ... while biotin-labeled goat anti-mouse IgA Ab (1:5000, Southern Biotech, Cat#1040-08) plus HRP-conjugated streptavidin (1:7000 ...
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bioRxiv - Microbiology 2024Quote: ... cells were labeled with 2 μg/ml PE-conjugated goat anti-human IgG (Southern Biotech) for 30 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... and permeabilized prior to staining with a FITC-conjugated goat anti-rhesus IgG (H+L) polyclonal antibody (Southern Biotech). Data represent the frequency of cells positive for IgG-binding to Env for post-vaccination samples compared to the pre-vaccination sample ...
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bioRxiv - Immunology 2022Quote: ... cryopreserved PBMC were thawed and stained with the following antibodies (monoclonal unless indicated): IgD FITC (goat polyclonal, Southern Biotech), IgM PerCP-Cy5.5 (clone G20-127 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then surface stained with mouse anti-chicken Bu-1-FITC (Clone AV20; Southern Biotech, Birmingham, AL, USA) and mouse anti-chicken monocyte/macrophage-PE (Clone KUL01 ...
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bioRxiv - Immunology 2024Quote: ... with serum diluted 1:10 followed by an incubation with goat anti-mouse IgG-FITC (1:100; Southern Biotech). Images were acquired on an inverted fluorescence microscope (Olympus) ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were stained again for 30 min with 0.5 µg of FITC-conjugated anti-mouse IgA antibody (Southern Biotech) in 100 µl of staining buffer to refresh and harmonize the staining between batches ...
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bioRxiv - Immunology 2023Quote: ... cryopreserved PBMC were thawed and stained with the following antibodies (monoclonal unless indicated): IgD FITC (goat polyclonal, Southern Biotech), IgM PerCP-Cy5.5 (clone G20-127 ...
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bioRxiv - Microbiology 2024Quote: ... Antibodies used for flow cytometry analysis performed in this study were: FITC-conjugated anti-human IgM (Southern Biotech #2020-02), goat anti-human IgG-UNLB (Southern Biotech #2040-01) ...
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bioRxiv - Immunology 2024Quote: ... Samples were resuspended in 100 µL binding buffer supplemented with 5 µL of Annexin V-FITC (Cat#: 10040-02, SouthernBiotech) and stained for 20 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... The yeast cells were washed twice in PBSF (1x PBS, 0.1% [w/v] BSA) before staining with anti-human LC-FITC (Southern Biotech), Streptavidin 633 (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... B cells were stained with anti-chicken Bu1-FITC clone AV20 (2.5 µg/ml) antibody (Southern Biotech, Alabama, United States), and live/dead staining was carried out with the 7-AAD-Viability Staining Solution (5 µg/ml ...
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bioRxiv - Immunology 2022Quote: ... all sourced from BEI Resources) and an Alexa Fluor 647 labeled goat anti-rabbit IgG (Southern Biotech) secondary in addition to Aqua (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... or goat whole IgG containing anti-monkey IgA (Novus) followed by phycoerythrin-labeled neutralite avidin (Southern Biotech). Fluorescence was measured with a Bio-Rad Bioplex 200 ...
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bioRxiv - Microbiology 2023Quote: ... washed then incubated for 1 hour with phycoerythrin labeled anti-human IgG Fc (Southern Biotech, Birmingham AL) detection antibody ...
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bioRxiv - Immunology 2022Quote: ... and biotin-labeled goat anti-mouse IgA (Cat# 1040-08) were obtained from Southern Biotech (Birmingham, AL). HRP-conjugated anti-mouse IgG Fab (Cat# A9917 ...
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bioRxiv - Immunology 2020Quote: ... The following antibody cocktails were used for B cell (FITC anti-mouse CD43, Biolegend, 553270; PE Goat anti-mouse IgM, Southern Biotech, 1020-09 ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were tryprinized and resuspended in binding buffer at 72 hours post-transfection and incubated with Annexin V-FITC (Southern Biotech) and propidium iodide (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... 1×109 bacteria were washed with 1 ml ice-cold PBS and stained for 30 min at 4°C with 4 µg of FITC-conjugated anti-mouse IgA antibody (Southern Biotech) in 100 µl of staining buffer ...
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bioRxiv - Biochemistry 2023Quote: ... incubated with an appropriate peroxidase-labeled secondary antibody (anti-mouse IgG1-HRP #1070-05, Southern Biotech, Birmingham, USA) for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclei were labeled with 4’,6-Diamidino-2-Phenylindole (DAPI) and slides were mounted using Fluoromount mounting medium (SouthernBioTech). Z-stack photomicrographs of dorsal and ventral lower lumbar spinal cord were acquired at 10x magnification using a Nikon Eclipse Ti2 inverted confocal microscope ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed with 1% BSA-PBS and labeled with APC-conjugated goat anti-rat immunoglobulin antibody (Southern Biotech) at 25°C for 20 min ...
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bioRxiv - Microbiology 2023Quote: ... The cell nuclei were labeled with a DAPI stain present in the mounting media (DAPI Fluoromount-G, Southern Biotech). Images were acquired using an upright microscope (Echo Revolve ...
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bioRxiv - Immunology 2023Quote: ... antibody response to individual peptides was detected with different fluorescently labeled secondary antibodies (Goat anti-human IgM-AF488, SouthernBiotech, Cat # 2020-30 ...
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bioRxiv - Immunology 2023Quote: ... The IgG assay contained a goat anti-mouse IgG Fc antibody (SouthernBiotech, 1033-31, labeled with Alexa Fluor 488) and the IgM assay a donkey anti-mouse IgM F(ab’)2 (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2020Quote: ... followed by two rounds of fluorescence-activated cell sorting (FACS) of cells stained with FITC-Conjugated rat anti-mouse IgG1 (Southern Biotech 1144-02), and rounds of soft-agar cloning in SeaPlaque agarose ...
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bioRxiv - Immunology 2021Quote: ... Wells were then washed 3 times with 200µL PBST before incubation for 1 hour with secondary antibodies (HRP-labeled Goat anti-mouse IgG1/IgG2b/IgG2c purchased from SouthernBiotech or HRP-labeled Goat anti-mouse IgG Fcγ purchased from Jackson ImmunoResearch ...
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bioRxiv - Immunology 2023Quote: ... antibody response to individual peptides was detected with different fluorescently labeled secondary antibodies (Goat anti-human IgM-AF488, SouthernBiotech, Cat # 2020-30; Goat anti-human IgG-PE, SouthernBiotech, Cat # 2040-09 ...
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bioRxiv - Neuroscience 2024Quote: ... as the capturing reagent and an Alexa-fluor® 647 labeled anti-human IgG (Cat no. 2049-31; Southern Biotech) as the detection reagent ...
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bioRxiv - Immunology 2024Quote: ... PE-labeled detector antibody (mouse anti- human IgG-Fc; Southern Biotech, Cat#9040–09, clone JDC-10; 1 μg/ml) was added and beads were incubated at room temperature for 1h on a shaker ...
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bioRxiv - Microbiology 2023Quote: ... Pellet was then resuspended in 100 µL ice-cold PBS containing 4 µg of FITC-coupled goat anti-mouse IgA antibody (SouthernBiotech, Imtec Diagnostic, #1040-02). The ratio of 4 µg of the respective antibody to stain 109 bacteria was previously evaluated to be the maximum amount of antibody that can be used without resulting in unspecific staining of non-IgA coated bacteria by using fecal samples from Rag1−/− mice as non IgA-coated negative controls ...
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bioRxiv - Microbiology 2020Quote: ... Antibody binding was detected using directly labeled IgG or Alexa Fluor647-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech). Samples were measured using flow cytometry (BD FACSVerse ...
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bioRxiv - Immunology 2022Quote: ... Bound IgG antibodies were detected with a horseradish peroxidase (HRP)-labeled secondary antibody (cat. no.: 2040-05, SouthernBiotech, Birmingham, AL, USA) and the addition of the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB ...
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bioRxiv - Cell Biology 2024Quote: ... Labeled cells were washed three times with TBS before being covered with Fluoromount mounting medium (Southern Biotech, Biozol Diagnostica, Eching, Germany). Photos were taken using confocal laser scanning microscopy (TCS SPEII ...
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bioRxiv - Biochemistry 2022Quote: ... labeled protein was visualized using the AP substrate 5-bromo-4-chloro-indolyl-phosphate/nitro blue tetrazolium (BCIP/NBT; Southern Biotech).
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bioRxiv - Immunology 2022Quote: ... The microtiter plates were developed with horseradish peroxidase (HRP)-labeled goat anti-human IgG or IgM polyclonal antibodies (SouthernBiotech, Birmingham, AL). The plates were developed with 3,3 ...
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bioRxiv - Bioengineering 2022Quote: ... The blots were washed with PBS and further developed with goat anti-rabbit IgG-horseradish peroxidase labeled (HRP) (Southern Biotech #4050-05) diluted 1:1000 for 1 h at RT ...
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bioRxiv - Immunology 2020Quote: ... plates were subsequently incubated with 0.1 µg/mL HRP-labeled goat anti-mouse κ light chain Ab (Southern Biotech, Birmingham, AL 35260), diluted in assay buffer for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... followed by 1 hr incubation with horseradish peroxidase-labeled goat anti-mouse secondary antibody (GAM-HRP, SouthernBiotech Cat#1010-05, RRID: AB_2728714). After washing ...
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bioRxiv - Genetics 2024Quote: ... The following antibody mixtures were used for B cell (FITC rat anti-mouse CD43, BD Pharmingen 553270; PE goat anti-mouse IgM, Southern Biotech 1020-09; PE-Cy5 anti-Hu/Mo CD45R (B220), eBioscience 15-0452-83 ...