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Citations for SouthernBiotech :
851 - 877 of 877 citations for Tetratricopeptide repeat protein 14 TTC14 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Cells were then washed with PBS-BSA and stained with secondary antibodies (5 μM Alexa Fluor® 488 anti-mouse (SouthernBiotech; 1031-30) and 10 μM Alexa Fluor® 647 anti-human (SouthernBiotech ...
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bioRxiv - Microbiology 2021Quote: ... samples were further statically incubated for 1 h at 4°C with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech, 1:300) and 6 μM Syto9 (Live/Dead BacLight Bacterial Viability Kit ...
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bioRxiv - Bioengineering 2021Quote: ... The slides were subsequently incubated at room temperature with secondary antibodies: goat anti-rabbit Ig (1:100 dilution for 15 minutes, 4010-05, Southern Biotech, Birmingham, AL) for CD4 and goat anti-rabbit (1:50 dilution for 30 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... Antibodies bound to the thymocytes were detected by Cy5-conjugated goat anti-mouse IgG (1:250 dilution, 1030-15, Southern Biotech, Birmingham, AL) and Alexa Fluor 488-conjugated goat anti-mouse IgM (1:250 dilution ...
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bioRxiv - Immunology 2021Quote: ... For detection of IgG subclasses all serum samples were diluted to 1 total IgG EU and incubated at 37 °C for 1 h prior to detection with Alkaline Phosphatase conjugated anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam) incubated for 1 h at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... all serum samples were diluted to 1 total IgG ELISA unit and then detected with anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam). The results of the IgG subclass ELISA are presented using OD values instead of the ELISA units used for the total IgG ELISA ...
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bioRxiv - Immunology 2022Quote: ... and stained with a 1:1,000 solution of goat anti-human IgG antibodies conjugated to phycoerythrin (PE) (Southern Biotech, Cat# 2040-09; RRID:AB_2795648) at 4°C for 30 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Staining was performed using anti-PrP antibody (clone ICSM18; D-Gen Ltd) followed by detection with alkaline-phosphatase-linked anti-IgG1 antiserum (Southern Biotech; 1070-04). Spots were visualised with alkaline phosphatase conjugate substrate (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: Biotinylated Goat F(ab)2 anti-human isotype antibodies (anti-human IgM, SouthernBiotech, Cat # 2022-01; anti-human IgA, SouthernBiotech, Cat # 2052-01, and anti-human IgG, SouthernBiotech, Cat # 2042-01) were conjugated on to selected peaks of Streptavidin coated 4um polystyrene capture beads ...
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bioRxiv - Immunology 2023Quote: ... and incubated with 0.75x serial dilutions of purified polyclonal human antibodies (IgG, SouthernBiotech, Cat # 0150-01; IgM, SouthernBiotech, Cat # 0158L-01; IgA, SouthernBiotech, Cat # 0155K-01) at known concentrations ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed and incubated with either AP-conjugated goat anti-mouse IgG or IgM antibody (SouthernBiotech; cat # 1030-04, 1020-04) (Supplementary Table 8) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated for 1 hour at room temperature in TBSX with 1:1000 donkey fluorescent secondary antibody and mounted on slides using Fluoromount-G (Southern Biotech 0100-01) before coverslipping ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The secondary antibodies were goat anti-rabbit (#4010-05, 1:5000) and anti-mouse (#1030-05, 1:5000, both from Southern Biotech, Birmingham AL) coupled to horseradish peroxidase ...
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bioRxiv - Immunology 2024Quote: ... 100 μl per well of 1 μg/l alkaline phosphatase (AP)-conjugated goat anti-human antibodies (Goat Anti-Human kappa-AP, SouthernBiotech, ref 2060-04) was incubated in PBS with 0.05% Tween® 20 for 2 h at 37°C ...
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bioRxiv - Immunology 2024Quote: ... coated overnight at 4°C (100 μl per well) with 2 μg/ml Igκ antibodies (Goat Anti-Human kappa-UNLB, SouthernBiotech, ref 2060-01) in PBS ...
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bioRxiv - Immunology 2024Quote: ... membranes were incubated with indicated primary antibodies overnight at 4 °C and with horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (SouthernBiotech, Birmingham, AL, USA) for 2 hours at room temperature ...
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bioRxiv - Immunology 2024Quote: Fifty microliters of appropriately diluted serum samples were added to 96-well plates precoated with anti-mouse Igκ and Igλ antibodies (2 mg/ml; Southern Biotech, Birmingham, AL) in 0.1 M carbonate buffer (pH 9.0) ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed with water three times before applying 25 μl of secondary antibody (goat anti-human IgG Fc-Southern Biotech, 2048-04; goat anti-mouse IgG Fc-Southern Biotech, 1033-04) at a dilution of 1:4,000 in blocking buffer ...
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bioRxiv - Immunology 2021Quote: ... Sections were washed in PBS before incubation with secondary antibodies (Table 3) for 1 hr at room temperature followed by mounting with DAPI containing fluoromount (Southern Biotech, Birmingham, AL, USA). Images were captured with an EVOS FL imaging system (ThermoFisher Scientific) ...
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bioRxiv - Physiology 2023Quote: ... The membranes were then washed 3x in TBST before incubation for 1 hour at room temperature with peroxidase-conjugated secondary antibodies in TBST at 1:10,000 (Southern Biotech, #1031-05, #4050-05). Antibody binding was detected using an enhanced chemiluminescence HRP substrate detection kit (Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... Paraffin sections of kidneys were used to stain for collagen type IV using a goat polyclonal collagen IV antibody (Southern Biotech, Birmingham, AL, USA) (Thallas-Bonke et al. ...
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bioRxiv - Microbiology 2023Quote: ... Pellet was then resuspended in 100 µL ice-cold PBS containing 4 µg of FITC-coupled goat anti-mouse IgA antibody (SouthernBiotech, Imtec Diagnostic, #1040-02). The ratio of 4 µg of the respective antibody to stain 109 bacteria was previously evaluated to be the maximum amount of antibody that can be used without resulting in unspecific staining of non-IgA coated bacteria by using fecal samples from Rag1−/− mice as non IgA-coated negative controls ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated in the secondary antibody at room temperature and washed with TBS prior to slide-mounting with DAPI Fluoromount-G® mounting medium (SouthernBiotech, catalog #0100-20). Primary antibodies included rabbit anti-DsRed (Takara Bio ...
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bioRxiv - Immunology 2024Quote: ... The plates were washed before the addition of goat anti-mouse IgG secondary antibody conjugated with alkaline phosphatase (AP) (Southern Biotech, Birmingham, AL, USA) for 2 hr ...
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bioRxiv - Microbiology 2021Quote: For the detection of antibody titers in sera and fecal supernatants 96-well plates were coated with goat anti-human Ig (H+L chain) antibody (Southern Biotech, Cat. No. 2010-01) or goat anti-human IgA Fab (Southern Biotech ...
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bioRxiv - Microbiology 2024Quote: ... Cells were incubated cell side down in 100 µL of Stellaris RNA FISH Hybridization Buffer (BioSearch) containing M1 vRNA probes (1/100) and the primary antibodies of interest (NP, Southern Biotech, 10770-01, 1/100) and incubated in a humid chamber at 37°C in the dark for 16 h ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed with 1% BSA in PBS twice before incubating with secondary anti-rat IgG antibody conjugated with APC (1:500; Southern Biotech, AL, USA, Catalog no. 3010-11L). Cells were analyzed in BD FACSVerse™ flow cytometer (BD Biosciences ...