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Citations for SouthernBiotech :
801 - 850 of 1199 citations for Goat anti guinea pig IgG 490 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The SOSIP-specific IgG response in the serially diluted RM plasma was measured using an anti-monkey IgG horseradish peroxidase (HRP)-conjugated secondary antibody at 1:4000 dilution (Southern Biotech, Birmingham, AL) and concentration of the CH505-SOSIP-specific IgG response was estimated using a standard curve of CH505 T/F SOSIP IgG detection by Rh HIV-specific monoclonal antibody ...
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bioRxiv - Cell Biology 2020Quote: ... samples were incubated for 30 minutes with the following secondary antibodies diluted in PBS 2.5% BSA: donkey anti-goat Alexa Fluor 488 1:200 (Southern Biotech 3425-30) and donkey anti-rabbit Alexa Fluor 555 1:200 (Thermo fisher scientific A32794) ...
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bioRxiv - Microbiology 2022Quote: ... the beads were incubated with 1 µg/mL Alexa Fluor647-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech, 2062-31) in PBS-TH for 30 min at 4 °C with shaking ...
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bioRxiv - Developmental Biology 2022Quote: ... then incubated in fresh dilution solution with secondary antibody (goat anti-mouse IgG2a AlexaFluor 488, 1:250, Southern Biotech, cat# 1080-30) overnight at 4°C with gentle shaking ...
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bioRxiv - Immunology 2019Quote: ... 4 × 106 primary B cells in 0.1 ml of imaging medium were stimulated with 20 µg/ml soluble goat anti-Igκ antibodies (Southern Biotech, #1050-01). Cells were lysed in RIPA buffer (30 mM Tris-HCl ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were washed in phosphate buffered saline containing 0.05% Tween 20 (PBST) and incubated with 10% rabbitserum and then goat anti-type V collagen antibody (Southern Biotech, Birmingham, AL) at 1:1000 for 1.5 hours ...
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bioRxiv - Microbiology 2020Quote: ... washed 3 times in ice-cold PBSM and then bound antibody detected with goat anti-human kappa-A647 (SouthernBiotech, cat# 2060-31) After washing ...
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bioRxiv - Immunology 2020Quote: ... plates were subsequently incubated with 0.1 µg/mL HRP-labeled goat anti-mouse κ light chain Ab (Southern Biotech, Birmingham, AL 35260), diluted in assay buffer for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... followed by 1 hr incubation with horseradish peroxidase-labeled goat anti-mouse secondary antibody (GAM-HRP, SouthernBiotech Cat#1010-05, RRID: AB_2728714). After washing ...
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bioRxiv - Immunology 2020Quote: ... washed 6 times and then incubated with 1: 6000 dilution of horseradish peroxidase (HRP) conjugated anti-mouse IgG secondary antibody (Southern Biotech; Birmingham, AL, USA) for 1 h at RT ...
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bioRxiv - Microbiology 2022Quote: ... Normal rabbit IgG (SouthernBiotech, Cat# 0111-01, 1:100) was used as negative controls ...
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bioRxiv - Immunology 2021Quote: ... HRP- conjugated IgG or IgM (Southern Biotech, Birmingham, AL) was incubated on the plate for 1 h at 37°C ...
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bioRxiv - Immunology 2020Quote: ... IgG and/or IgM (Southern Biotech; Birmingham, AL, USA), was added at 3 μg/mL ...
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bioRxiv - Microbiology 2020Quote: ... IgG and/or IgM (Southern Biotech; Birmingham, AL, USA). After 30 minutes of incubation at 800 rpm in the dark ...
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bioRxiv - Microbiology 2022Quote: ... Normal rabbit IgG (SouthernBiotech, Cat# 0111-01, 1:100) was used as negative controls ...
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bioRxiv - Immunology 2020Quote: ... Biotinylated rabbit control IgG (Southern Biotech, Birmingham, AL, USA) was used as a negative control ...
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bioRxiv - Microbiology 2023Quote: ... Normal rabbit IgG (SouthernBiotech, Cat# 0111-01, 1:100) was used as negative control ...
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bioRxiv - Synthetic Biology 2023Quote: ... an IgG isotype control (Southern Biotech Catalog# 0109-30) to correct the MFI of AF488 for non-specific staining.
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bioRxiv - Cell Biology 2020Quote: ... Then samples were washed three times with 0.1% Triton X-100 in PBS for 5 minutes and were incubated 30 minutes at RT with the following secondary antibody diluted 1:200: goat anti-rabbit Alexa Fluor 488 (Southern Biotech 4050-30), goat anti-mouse Alexa Fluor 488 (Southern Biotech 1030-30 ...
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bioRxiv - Immunology 2021Quote: ... Unbound antibodies were washed away with PBST and bound antibodies detected with 0.5 μg/mL HRP-conjugated goat anti-human constant kappa (2060-05, Southern Biotech, Birmingham, AL, USA) diluted in PBSTB ...
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bioRxiv - Microbiology 2021Quote: ... samples were further statically incubated for 1 h at 4°C with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech, 1:300) and 6 μM Syto9 (Live/Dead BacLight Bacterial Viability Kit ...
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bioRxiv - Bioengineering 2021Quote: ... The slides were subsequently incubated at room temperature with secondary antibodies: goat anti-rabbit Ig (1:100 dilution for 15 minutes, 4010-05, Southern Biotech, Birmingham, AL) for CD4 and goat anti-rabbit (1:50 dilution for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Plates were washed five times with PBS before adding 100 uL of HRP-conjugated goat anti-mouse IgG1 (Southern Biotech, Birmingham, AL, USA) diluted 1:4000 in blocking buffer and were then incubated overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... standards (IgM and IgG from Southern Biotech; IgE from Biolegend) and samples were applied to the coated plate for 1 hr at room temperature (RT) ...
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bioRxiv - Microbiology 2022Quote: ... Normal rabbit IgG (Southern Biotech, Cat# 0111-01, 1:100) was used as a negative control ...
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bioRxiv - Microbiology 2022Quote: ... Normal rabbit IgG (Southern Biotech, Cat# 0111-01, 1:100) was used as a negative control ...
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bioRxiv - Microbiology 2023Quote: ... Normal rabbit IgG (Southern Biotech, Cat# 0111-01, 1:100) was used as a negative control ...
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bioRxiv - Microbiology 2023Quote: ... Normal rabbit IgG (Southern Biotech, Cat# 0111-01, 1:100) was used as a negative control ...
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bioRxiv - Immunology 2020Quote: ... Horse spleen ferritin-specific IgA levels in the supernatant of the faecal homogenates was determined by enzyme-linked immunosorbent assay in plates coated with horse spleen ferritin followed by detection with horseradish peroxidase-conjugated goat anti-mouse IgA (Southern Biotech, Birmingham, AL, USA) using optEIA (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... 96-well Immulon 4 HBX plates were coated overnight at 4°C with 5 mg/ml polyclonal goat-anti mouse κ (Southern Biotech; 1050-01). Nonspecific binding was blocked with 1% bovine serum albumin in PBS and samples were incubated at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Pellet was then resuspended in 100 µL ice-cold PBS containing 4 µg of FITC-coupled goat anti-mouse IgA antibody (SouthernBiotech, Imtec Diagnostic, #1040-02). The ratio of 4 µg of the respective antibody to stain 109 bacteria was previously evaluated to be the maximum amount of antibody that can be used without resulting in unspecific staining of non-IgA coated bacteria by using fecal samples from Rag1−/− mice as non IgA-coated negative controls ...
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bioRxiv - Molecular Biology 2020Quote: ... and goat α-rabbit-HRP (Southern Biotech, 1:5000).
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bioRxiv - Immunology 2019Quote: ... A secondary polyclonal goat antibody (Southern Biotech, Birmingham, AL) directed to the human kappa light chain and conjugated with horseradish peroxidase (HRP ...
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bioRxiv - Microbiology 2020Quote: ... 1:250 ColIV (polyclonal goat, 1340-01, Southern Biotech), 1:250 CD31 (monoclonal mouse ...
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bioRxiv - Immunology 2023Quote: ... After blocking with 10% goat serum (SouthernBiotech; Birmingham, AL), samples were incubated with mouse anti-PI(3,4)P2 mAB or mouse anti-PI(3,4,5)P3 mAB conjugated with FITC for 1 hour at 37°C [24] ...
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bioRxiv - Bioengineering 2023Quote: ... (2) block sections in 5% normal goat serum (SouthernBiotech) and 0.3% Triton-X solution in PBS for 1h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... FO B cells and MZ B cells were cultured in RPMI medium + Pen/Strep + L-glutamine + 10% FBS and stimulated with polyclonal goat anti-mouse IgM (Southern Biotech; 0.6-150 ug/mL), or equimolar concentrations of the Fab’2 fragments (Southern Biotech ...
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bioRxiv - Microbiology 2021Quote: For the detection of antibody titers in sera and fecal supernatants 96-well plates were coated with goat anti-human Ig (H+L chain) antibody (Southern Biotech, Cat. No. 2010-01) or goat anti-human IgA Fab (Southern Biotech ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed with 1% BSA in PBS twice before incubating with secondary anti-rat IgG antibody conjugated with APC (1:500; Southern Biotech, AL, USA, Catalog no. 3010-11L). Cells were analyzed in BD FACSVerse™ flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: Antigen-specific IgG (Cat. No. 1015-05, Southern Biotech, AL, USA), IgA (Cat ...
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bioRxiv - Bioengineering 2021Quote: ... Wells were incubated with 100μL IgG-biotin HU adsorbed (Southern Biotech) for 2hr at RT ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and blocked with unlabeled mouse IgG (Southern Biotech) prior to subsequent staining and analysis.
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bioRxiv - Immunology 2021Quote: To detect mouse Ig Nunc MaxiSorp ELISA plates were coated for 1h at room temperature with goat anti-mouse Ig (H+L) (4 μg/ml; Southern Biotech, Cat#1010-01; RRID: AB_2794121). Citrobacter rodentium-specific Ig was quantified by coating Nunc MaxiSorp ELISA plates with 108 bacteria/well heat-inactivated at 60°C for 1 h ...
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bioRxiv - Bioengineering 2023Quote: ... The slices were blocked in 10% normal goat serum (SouthernBiotech) and 0.3% Triton-X solution in PBS for 1h at room temperature and then incubated with a primary rabbit anti-RLuc antibody (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation with HRP- conjugated IgM or IgG Ab (Southern Biotech). SureBlue substrate (KPL ...
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bioRxiv - Bioengineering 2021Quote: ... Wells were incubated with 100 µL IgG-biotin HU adsorbed (Southern Biotech) for 2 h at RT ...
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bioRxiv - Immunology 2022Quote: ... Standard curves were generated using unlabeled mouse IgG (Southern Biotech #0107-01), and detection was performed with anti-mouse IgG-HRP (Southern Biotech) ...
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bioRxiv - Microbiology 2023Quote: ... followed by incubation with HRP-conjugated IgM or IgG Ab (Southern Biotech). SureBlue substrate (KPL ...
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bioRxiv - Microbiology 2023Quote: ... horseradish peroxide (HRP) conjugated secondary antibodies for measurement of total IgG (SouthernBiotech) and individual IgG subclasses 1-4 (SouthernBiotech ...
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bioRxiv - Bioengineering 2023Quote: ... Wells were incubated with 100 µL IgG-biotin HU adsorbed (Southern Biotech) for 2 hr at RT ...