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Citations for SouthernBiotech :
751 - 800 of 848 citations for Solute Carrier Family 12 Member 1 SLC12A1 Antibody APC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Cells were washed with 1x PBS and incubated with 1:4000 anti-human IgG-HRP (Southern Biotech) for 1h at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... followed by addition of 50 µL of 1:1,000 anti-mouse IgG-HRP (Southern Biotech Cat. #1030-05) in PBST or 1:1000 of anti-mouse IgA-HRP in PBSTBA (SouthernBiotech) ...
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bioRxiv - Immunology 2021Quote: ... HRP-conjugated mouse anti-histidine-tag IgG (1:4000) (catalog number 4603-05, Southern Biotech, Birmingham, AL, USA) was added for 1 hour followed by addition of TMB substrate ...
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bioRxiv - Immunology 2020Quote: ... plates were incubated with anti-mouse IgG secondary Ab (Southern Biotech, cat no 1170-05, dilution 1:1000) 1hr at RT ...
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bioRxiv - Bioengineering 2021Quote: ... and Alexa Fluor 488-conjugated goat anti-mouse IgM (1:250 dilution, 1020-30, Southern Biotech, Birmingham, AL) for 30 minutes at 4°C and analyzed in a BD FACSCanto II (BD Biosciences ...
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bioRxiv - Bioengineering 2021Quote: ... for CD4 and goat anti-rabbit (1:50 dilution for 30 minutes, 4010-05, Southern Biotech, Birmingham, AL) for CD8 ...
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bioRxiv - Microbiology 2020Quote: ... followed by addition of 50 µL of 1:2000 anti-mouse IgG-HRP (Southern Biotech Cat. #1030-05) in PBST or 1:10000 of biotinylated anti-mouse IgG ...
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bioRxiv - Immunology 2021Quote: ... and incubated with chicken IgY anti-DMPO (20μg/mL) and rat IgY anti-mouse CD8a (1:250, SouthernBiotech). DMPO adducts were detected with Alexa Fluor 488–conjugated goat anti-chicken IgY secondary antibody (1:400 ...
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bioRxiv - Biochemistry 2020Quote: ... diluted 1:1,000 in TBST followed by 30 min with rabbit anti-goat HRP IgG (SouthernBiotech, #6164-05) at a dilution of 1:2,000 ...
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bioRxiv - Immunology 2020Quote: ... was coated with a 1:500 dilution of unlabeled goat anti-human IgG (Southern Biotech, Birmingham, AL, USA) and incubated at 37°C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... for C5b6 with a 1:5.000 of HRP-conjugated donkey antisera against goat IgG (H+L) (Southern Biotech) and for sMAC with 1:5,000 HRP-conjugated streptavidin (Southern Biotech) ...
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bioRxiv - Microbiology 2023Quote: ... followed by addition of 50 μL of 1:2000 anti-mouse IgG-HRP (Southern Biotech, Cat. #1030-05), or anti-mouse IgA- biotin (Southern Biotech ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed with PBS and incubated for 1 h with a secondary goat anti-mouse FITC (SouthernBiotech) at a 20 μg/mL concentration and phalloidin (7 units ...
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bioRxiv - Genomics 2023Quote: ... Samples were washed twice with 1×PBST and mounted onto clean microscope slides using Fluoromount G (Southern Biotech). Images were acquired with a Nikon A1 RMP confocal using a 60×/1.49 oil immersion objective ...
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bioRxiv - Molecular Biology 2023Quote: ... were diluted 1:1000 in PBS and precomplexed with goat anti-mouse IgM-HRP (#1021-05, Southern Biotech) in a 1:100 dilution on ice for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... a rabbit anti-rat conjugated to horseradish peroxidase was used as a secondary Ab (1:1000; Southern Biotech) and incubated with TBST (5% BSA ...
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bioRxiv - Immunology 2022Quote: ... Plates were then washed and incubated with 50μL of anti-mouse IgA-HRP (SouthernBiotech, 1:1000 in PBT), washed and developed using 50 μL of TMB substrate (BioLegend) ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed and incubated with 50μL of anti-mouse IgA-HRP (1:1000) in assay buffer (SouthernBiotech). Plates were washed and developed using TMB substrate (BioLegend) ...
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bioRxiv - Microbiology 2024Quote: ... followed by addition of 50 μL of 1:2000 anti-mouse IgG-HRP (Southern Biotech Cat. #1030-05) in PBST ...
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bioRxiv - Immunology 2023Quote: ... and incubated for 1 hour at room temperature with horseradish peroxidase–conjugated goat anti–mouse IgG (Southern Biotech) diluted 1:4000 with assay diluent ...
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bioRxiv - Biochemistry 2023Quote: ... plates were coated with 1:1000 dilution each of goat anti-mouse Kappa (Catalog #1050-01, Southern Biotech) and Lambda light chains (Catalog #1060-01 ...
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bioRxiv - Immunology 2023Quote: ... Membranes were then incubated with HRP-conjugated goat anti-rabbit IgG (H+L) (1:4000 dilution; Southern Biotech) at RT for 1 h ...
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bioRxiv - Immunology 2023Quote: ... the plate was incubated with HRP-conjugated goat anti-mouse IgG (1:4000) (Southern Biotech, Birmingham, AL, USA) and color reaction was developed by adding TMB (Invitrogen™ ...
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bioRxiv - Microbiology 2021Quote: ... secondary staining was performed for C3a with 1:5,000 HRP-conjugated polyclonal goat antisera against rabbit IgG (Southern Biotech), for C5b6 with a 1:5.000 of HRP-conjugated donkey antisera against goat IgG (H+L ...
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bioRxiv - Immunology 2022Quote: ... Plate was then washed five times with PBS-T and developed for 1 minute at room temperature using 3,3’,5,5’-tetramethylbenzidine (TMB) substrate (Southern Biotech). Reactions were stopped with 2 M HCl ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then surface stained with mouse anti-chicken Bu-1-FITC (Clone AV20; Southern Biotech, Birmingham, AL, USA) and mouse anti-chicken monocyte/macrophage-PE (Clone KUL01 ...
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bioRxiv - Immunology 2021Quote: ... followed by the addition of horseradish peroxidase (HRP)-conjugated goat anti-Hamster IgG (H+L) (1:2000 dilution) (SouthernBiotech) for an hour at RT ...
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bioRxiv - Immunology 2021Quote: ... RBC-depleted splenocytes were incubated with 1 μg/mL of goat F(ab’)2 anti-mouse Igκ-biotin (SouthernBiotech) for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... a GAM IgG (H + L) conjugated to horseradish peroxidase was used as a secondary Ab (1:1000; Southern Biotech) and incubated with TBST (5% BSA ...
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bioRxiv - Immunology 2021Quote: ... plates were washed and probed at room temperature for 1 hour with 1μg/ml of biotinylated anti-IgG (Southern Biotech) in PBS 2.5%FCS ...
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bioRxiv - Microbiology 2021Quote: ... Secondary staining was performed with a 1:10,000 dilution of HRP-conjugated pooled donkey antisera against goat IgG (H+L) (Southern Biotech) in PBS-T supplemented with 1% ELK for 60 minutes at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... followed by addition of 50 μL of 1:1000 anti-hamster-IgG(H+L)-HRP (Southern Biotech Cat. #6061-05) in PBST or PBSTF or 1:1000 anti-hamster-IgG2/IgG3-HRP in PBST or PBSTF (Southern Biotech Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by detection with a 1:10,000 dilution of goat anti-human IgG-HRP (Southern Biotech 2040-05, Birmingham, AL). Plates were developed for 5 minutes with TMB substrate (VWR 95059-286) ...
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bioRxiv - Microbiology 2020Quote: ... membranes were washed with TBST and imaged (for Strep-Tag II HRP) or incubated with goat anti-rabbit-HRP (1:5,000; Southern Biotech).
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bioRxiv - Biochemistry 2020Quote: ... each well was incubated with 50 μL diluted 1:5,000 in BSA5-PBS-T of anti-rabbit-HRP (SouthernBiotech, USA) or anti-mouse-HRP (Rockland Immunochemicals ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were stimulated with buffer (1% FBS/HBSS) or 10 μg/mL goat F(ab’)2 anti-mouse Igκ (SouthernBiotech) and Indo-1 fluorescence (violet vs blue ...
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bioRxiv - Immunology 2020Quote: ... bound IgG was detected by incubating with 1:1000 polyclonal goat anti human IgG-HRP (Southern Biotech, Birmingham, AL, USA) for 1h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... All coverslips were then washed 3 times in PBS before being mounted on a drop of mounting medium (DAPI fluoromount – G 1:1000; SouthernBiotech)) on labeled glass coverslips ...
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bioRxiv - Microbiology 2022Quote: ... the plate was incubated in goat anti-mouse horseradish peroxidase (GAM-HRP) at a 1:5000 ratio (SouthernBiotech, Birmingham, AL). Lastly ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed 3x with DPBS and coverslips mounted on 1 mm glass slides using Fluoromount-G (SouthernBiotech, #0100-01). Cells were imaged using a Zeiss Axio Observer 7 fitted with a 40x objective using DAPI and Alexa Fluor 488 filters ...
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bioRxiv - Neuroscience 2024Quote: ... Coverslips were washed in 1× PBS and mounted on glass slides with Fluoromount G (Southern Biotech, with or without DAPI).
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bioRxiv - Immunology 2024Quote: ... Membranes were incubated in TBST containing 5% (w/v) skimmed milk with goat anti-mouse IgG conjugated to horseradish peroxidase (HRP) (1:2000, 1010-05, SouthernBiotech) for 3h at 4°C in a rotary platform and washed thrice with TBST for 10 min each time ...
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bioRxiv - Immunology 2024Quote: ... wells were washed six times with PBS supplemented with 0.1 % Tween20 and incubated for 2 h with 1 μg/mL biotinylated goat α-mouse IgM or IgG1 (SouthernBiotech) at 37 °C ...
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bioRxiv - Bioengineering 2023Quote: ... The staining procedure involved the following steps: (1) The sections were blocked in a solution of 10% normal goat serum (SouthernBiotech) and 0.3% Triton-X in PBS for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... 0,1 % BSA and washed three times with 1× PBS before mounting on microscope slides using Fluoromount-G (Cat# 0100-01, SouthernBiotech,). Hoechst 33342 dye (Cat# B2261 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 × 105 cells in 100 μL per well were plated in a 96-well plate pre-coated with anti-mouse CD3ε (1 μg/mL, SouthernBiotech). Tumors were harvested from mice 10 days post inoculation and dissociated using gentleMACS (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... A volume of 25 μL of a 1:1,000 dilution of Mouse Anti-Biotin-AP (Southern Biotech, cat. #6404-04) in blocking buffer was added to each of the wells of the HN plates and incubated for 1 hr at RT ...
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bioRxiv - Immunology 2024Quote: ... 25 μl of undiluted supernatant was added to wells coated with 1 μg/ml goat anti-mouse Ig (Southern Biotech) or HAPR8 and for serum samples 3-fold serial dilutions of serum samples starting at 1/100 were added to wells coated with NP4-BSA (10 μg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... the tissue was allowed to dry on the slides at 4 °C and then briefly re-wet with 1 M PBS before being covered with DAPI Fluoromount-G (SouthernBiotech) and coverslipped ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then stained with iamidino-2-phenylindole (DAPI; 1:10000) to label nuclei and mounted in Fluoromount-G Mounting Medium (SouthernBiotech). Images of whole sections were acquired with a Zeiss Axio ScanZ1 slide scanner using a 20x objective lens and Zen Blue 3.1 software (Carl Zeiss ...