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Citations for SouthernBiotech :
751 - 800 of 1163 citations for Mouse Anti Human Ig kappa chain Alexa Fluor 488 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... human/bovine/horse SP ads antibody (Southern Biotech) at a concentration of 1:4000 ...
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bioRxiv - Immunology 2021Quote: ... human/bovine/horse SP ads antibody (Southern Biotech) was applied at a concentration of 1:4000 and a volume of 150 μL as a secondary antibody ...
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bioRxiv - Immunology 2020Quote: ... purified human IgG (Southern Biotech, Birmingham, AL, USA) and purified IgG-ZE3 fusions were added at 0.1 mg/ml with 10-fold serial dilutions and incubated for 1.5 hours at 37°C ...
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bioRxiv - Genetics 2021Quote: ... Wells were washed as described and biotin conjugated goat anti-human IgG (Jackson AffiniPure #109-065-098; 1:30,000 or Southern Biotech 2049-08; 1:1,000) in PBS/1.0% BSA detection antibody was added to the wells at 100ul/well and incubated for 2 hours at room temperature with shaking ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed three times with PBS-T and secondary goat anti-mouse IgG-HRP antibody (Southern Biotech), diluted 1:4000 in ELISA buffer ...
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bioRxiv - Neuroscience 2021Quote: ... samples were labelled with biotin-coupled antibodies (all antibodies were purchased from Southern Biotech: goat anti mouse (1012-08), goat anti rat (3052-08) ...
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bioRxiv - Microbiology 2020Quote: ... Plates were again washed with PBST and coated with 50 μL 1:10,000 diluted secondary antibody goat anti-mouse IgG HRP conjugated (1030-05, SouthernBiotech) and incubated 1 hr at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Plates were then washed three times with PBS-T and secondary goat anti-mouse IgG-HRP antibody (Southern Biotech), diluted 1:4000 in ELISA buffer ...
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bioRxiv - Immunology 2021Quote: ... For the three-step protocol sections were stained using a goat anti-mouse IgG1-HRP-conjugated antibody (Southern Biotech) for CXCL12 and goat anti-mouse IgG2b-HRP-conjugated antibody (Southern Biotech ...
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bioRxiv - Immunology 2020Quote: ... HRP-conjugated secondary antibodies were used as follows: goat anti-mouse IgG-HRP (SouthernBiotech, Cat: 1030-05; 1:10,000) or goat anti-mouse IgA-HRP (SouthernBiotech ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then surface stained with mouse anti-chicken Bu-1-FITC (Clone AV20; Southern Biotech, Birmingham, AL, USA) and mouse anti-chicken monocyte/macrophage-PE (Clone KUL01 ...
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bioRxiv - Immunology 2020Quote: ... coated with 100 µL/well of goat anti-mouse IgA (5 µg/mL Bic 0.1M, pH 9.6; Southern Biotech) were incubated with serial 3-fold dilutions of either fecal supernatant (100 µL ...
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bioRxiv - Immunology 2021Quote: ... RBC-depleted splenocytes were incubated with 1 μg/mL of goat F(ab’)2 anti-mouse Igκ-biotin (SouthernBiotech) for 30 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... the plates were incubated with a 1:2,000 dilution of a goat anti-mouse IgG2a-HRP antibody (Southern Biotech) in PBS supplemented with 1% FCS and incubated with gently shaking at RT for one hour ...
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bioRxiv - Immunology 2021Quote: ... Bound Abs were then reacted with HRP-conjugated goat anti-mouse IgG or IgM (Southern Biotech Cat.#1030-05) at 37lJ for 20 min ...
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bioRxiv - Immunology 2022Quote: ... 2M7 mAb isolated from rabbit PBMCs was detected with an HRP-conjugated polyclonal mouse anti-rabbit IgG (Southern Biotech) and all the positive controls were detected with an HRP-conjugated polyclonal goat anti-human IgG (Southern Biotech ...
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bioRxiv - Microbiology 2023Quote: ... the plates were washed with washing buffer twice before adding the HRP-conjugated mouse anti-monkey IgG (Southern Biotech) and incubating at room temperature for 1 hour ...
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bioRxiv - Biophysics 2023Quote: ... at a 1:250 dilution and protein was detected with a HRP-conjugated goat anti-mouse antibody (Southern Biotech) using a 1:5000 dilution ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were stained again for 30 min with 0.5 µg of FITC-conjugated anti-mouse IgA antibody (Southern Biotech) in 100 µl of staining buffer to refresh and harmonize the staining between batches ...
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bioRxiv - Microbiology 2023Quote: ... 20 µl/well of a Phosphatase Alkaline-conjugated goat anti-mouse IgA (Southern Biotech, Imtec diagnostics, Ref: 1040-04), diluted 1/1000 in Dilution Buffer ...
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bioRxiv - Bioengineering 2024Quote: ... plates were washed with PBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG1 antibodies (Southern Biotech) or HRP-conjugated goat anti-mouse IgG2a-HRP antibodies (Southern Biotech ...
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bioRxiv - Bioengineering 2024Quote: ... Secondary antibody was horseradish peroxidase (HRP) conjugated anti-mouse IgG antibody and was procured from Southern Biotech (Birmingham AL). KPL TMB Stop Solution was purchased from Sera Care (Milford ...
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bioRxiv - Immunology 2024Quote: ... with serum diluted 1:10 followed by an incubation with goat anti-mouse IgG-FITC (1:100; Southern Biotech). Images were acquired on an inverted fluorescence microscope (Olympus) ...
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bioRxiv - Cancer Biology 2023Quote: ... Goat ani-human IgG HRP (1:5000, Southern Biotech) was used as secondary antibody ...
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bioRxiv - Immunology 2020Quote: ... The assay was performed as described above except for the secondary antibodies (1:2,000. goat Anti-Mouse IgG2a cross absorbed HRP antibody, Southern biotech, 1083-05 ...
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bioRxiv - Immunology 2021Quote: ... Wells were then washed 3 times with 200µL PBST before incubation for 1 hour with secondary antibodies (HRP-labeled Goat anti-mouse IgG1/IgG2b/IgG2c purchased from SouthernBiotech or HRP-labeled Goat anti-mouse IgG Fcγ purchased from Jackson ImmunoResearch ...
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bioRxiv - Immunology 2021Quote: ... then incubated with horseradish peroxidase (HRP)-conjugated mouse anti-monkey IgG (catalog number 4700-05, Southern Biotech, Birmingham, AL, USA) for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed 3X with PBS and 3X with PBS-T and then anti-mouse IgG/IgA-biotin (Southern Biotech) diluted 1:1000 in PBS-T was added and incubated overnight at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed 3x and incubated with a 1:50,000 dilution of anti-mouse total IgG-HRP antibody (Southern Biotech) and incubated for 1h at 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... and then for 1 hour at room temperature with a 1:4000 diluted rabbit anti-mouse HRP secondary antibody (SouthernBiotech Cat# 6170-05 ...
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bioRxiv - Immunology 2021Quote: ... at 1×106 cells/well and stimulated for 48 hours with 5 μg/mL of RBD-His or hCD81-His or media alone (RPMI + 5% FCS) in the presence of anti-mouse CD28 antibody [0,5 μg/ml] (Southern Biotech). As positive control cells were stimulated with anti-mouse CD3 [0,05 μg/ml] (Southern Biotech) ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were stimulated with buffer (1% FBS/HBSS) or 10 μg/mL goat F(ab’)2 anti-mouse Igκ (SouthernBiotech) and Indo-1 fluorescence (violet vs blue ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 5 times with PBS-T and incubated with goat anti-mouse IgG1 antibody conjugated with horseradish peroxidase (SouthernBiotech) for 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Bound IgM monoclonal antibody was detected with PE-labelled goat anti-mouse IgM secondary antibody (Southern Biotech, Birmingham, AL, US) (Halpin et al. ...
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bioRxiv - Immunology 2022Quote: ... A volume of 25 μL of a 1:1,000 dilution of Mouse Anti-Biotin-AP (Southern Biotech, cat. #6404-04) in blocking buffer was added to each of the wells of the HN plates and incubated for 1 hr at RT ...
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bioRxiv - Microbiology 2022Quote: ... the plate was incubated in goat anti-mouse horseradish peroxidase (GAM-HRP) at a 1:5000 ratio (SouthernBiotech, Birmingham, AL). Lastly ...
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bioRxiv - Immunology 2022Quote: ... The bound rabbit plasma IgG was detected with a horseradish peroxidase (HRP)-conjugated polyclonal mouse anti-rabbit IgG (Southern Biotech) and the CytoGam was detected with an HRP-conjugated polyclonal goat anti-human IgG (Southern Biotech ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed and the samples were incubated with 50 μl of streptavidin-HRP diluted in blocking solution and standards with 50 μl of 0.175 μg/ml rat anti-mouse IgE-HRP (1130-05, Southern Biotech) diluted in blocking solution for 1 hour at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... the arrays were incubated with a mixture of goat anti-mouse IgM HRP (10 µg/mL; #1021-05 Southern Biotech) and donkey anti-goat antibody labeled with AlexaFluor555 (5 µg/mL ...
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bioRxiv - Microbiology 2023Quote: ... Bound antibodies were detected by using goat anti-mouse IgG conjugated to horseradish peroxidase (HRP; Southern Biotech, Birmingham, AL, USA) diluted 1:2000 in PBST and developed using 3,3’,5,5’-tetramethylbenzidine substrate (Thermo Scientific Pierce ...
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bioRxiv - Microbiology 2023Quote: ... bound S8V1-157 was detected by incubating the microspheres with 2 μg/ml PE-conjugated rat anti-mouse IgG1 (SB77e, SouthernBiotech) for 1 hr at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 × 105 cells in 100 μL per well were plated in a 96-well plate pre-coated with anti-mouse CD3ε (1 μg/mL, SouthernBiotech). Tumors were harvested from mice 10 days post inoculation and dissociated using gentleMACS (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... Membranes were incubated in TBST containing 5% (w/v) skimmed milk with goat anti-mouse IgG conjugated to horseradish peroxidase (HRP) (1:2000, 1010-05, SouthernBiotech) for 3h at 4°C in a rotary platform and washed thrice with TBST for 10 min each time ...
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bioRxiv - Microbiology 2021Quote: ... F(ab’)2 fragments or human IgG1 (control) (Southern Biotech) for 1hr at 37°C in a 96-well plate ...
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bioRxiv - Immunology 2023Quote: ... Human ads-AP (Southern Biotech; Cat#1070-04; RRID: AB_2794411) PE-Cyanine7 Rat Anti-Mouse IgM (Clone II/41 ...
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bioRxiv - Immunology 2023Quote: ... Human ads-AP (Southern Biotech; Cat#1020-04; RRID: AB_2794200) Mouse Anti-HEL (R ...
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bioRxiv - Immunology 2021Quote: ... washing with wash buffer (1xPBS+0.05% Tween) and incubation with secondary antibody - Mouse-anti-monkey IgG-HRP (Southern Biotech, 4700-05) for 1h ...
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bioRxiv - Immunology 2021Quote: ... This will be followed by a 1 h incubation with biotinylated HRP conjugated goat anti-mouse IgG subtype antibodies (Southern Biotech). 3,3’,5,5’ tetramethylbenzidine (TMB ...
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bioRxiv - Immunology 2019Quote: ... Sera were incubated 2 h at 37°C on wells pre-coated with 5 μg/mL of NP15-BSA or goat-anti-mouse IgM (Southern Biotech) and saturated with PBS 2% BSA ...
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bioRxiv - Immunology 2019Quote: ... culture supernatants were incubated 2 h at 37°C on wells pre-coated with 5 μg/mL of goat-anti-mouse IgM (Southern Biotech) and saturated with PBS 2% BSA ...