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Citations for SouthernBiotech :
651 - 700 of 719 citations for Goat Interleukin 6 IL6 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... then incubated in fresh dilution solution with secondary antibody (goat anti-mouse IgG2a AlexaFluor 488, 1:250, Southern Biotech, cat# 1080-30) overnight at 4°C with gentle shaking ...
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bioRxiv - Immunology 2019Quote: ... 4 × 106 primary B cells in 0.1 ml of imaging medium were stimulated with 20 µg/ml soluble goat anti-Igκ antibodies (Southern Biotech, #1050-01). Cells were lysed in RIPA buffer (30 mM Tris-HCl ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed with 0.05% Tween-20 in PBS and further incubated with HRP-conjugated goat antibodies directed against mouse IgG (Southern Biotech, Birmingham, AL) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were washed in phosphate buffered saline containing 0.05% Tween 20 (PBST) and incubated with 10% rabbitserum and then goat anti-type V collagen antibody (Southern Biotech, Birmingham, AL) at 1:1000 for 1.5 hours ...
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bioRxiv - Microbiology 2020Quote: ... washed 3 times in ice-cold PBSM and then bound antibody detected with goat anti-human kappa-A647 (SouthernBiotech, cat# 2060-31) After washing ...
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bioRxiv - Immunology 2020Quote: ... plates were coated with 1:2000 (for capture of polyclonal macaque IgG) or 1:1000 (for capture of polyclonal human IgG) of goat anti-Human IgG λ and K (Southern Biotech). The next day ...
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bioRxiv - Immunology 2020Quote: ... plates were subsequently incubated with 0.1 µg/mL HRP-labeled goat anti-mouse κ light chain Ab (Southern Biotech, Birmingham, AL 35260), diluted in assay buffer for 1 hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... except constructs were bound directly to the plate and detected with goat anti-mouse IgG HRP conjugate (Southern Biotech, Birmingham, AL, USA).
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bioRxiv - Immunology 2021Quote: ... The secondary antibody used was horseradish-peroxidase (HRP)-conjugated goat polyclonal antibody against mouse IgG1 (1070-05; Southern Biotech, Cambridge, United Kingdom), HRP-conjugated goat polyclonal antibody against rabbit IgG (4050-05 ...
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bioRxiv - Immunology 2020Quote: ... plates were washed with PBS-T and HRP-conjugated goat anti-mouse IgG or anti-rat IgG (Southern Biotech, Birmingham, AL, USA) added for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... The next day slides were washed and incubated for 1 hr in a secondary biotin-conjugated anti-mouse antibody (1:200, goat anti mouse, SouthernBiotech, Birmingham, AL) mixed in blocking solution ...
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bioRxiv - Immunology 2023Quote: ... Plates were then incubated for 90min at RT with goat anti-mouse IgG conjugated to horseradish peroxidase (HRP) (Southern Biotech, #1030-05) at 1:5000 dilution in blocking solution ...
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bioRxiv - Immunology 2023Quote: ... MAB273 was detected by adding a 1:5,000 dilution of monkey cross-adsorbed polyclonal goat anti-human IgG HRP antibody (Southern Biotech, 2049-05) and the signal was developed as described above.
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bioRxiv - Bioengineering 2023Quote: ... followed by 1 hr incubation with horseradish peroxidase-labeled goat anti-mouse secondary antibody (GAM-HRP, SouthernBiotech Cat#1010-05, RRID: AB_2728714). After washing ...
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bioRxiv - Immunology 2024Quote: ... plates were washed four times and 25 µl of a 1:500 dilution of the respective detection antibody (goat anti-mouse IgG(H+L)-alkaline phosphatase (AP) conjugate (Southern Biotech/Biozol) or goat anti-mouse IgG1-AP (Southern Biotech/Biozol ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 5 times with PBS/0.05% Tween20 and IgG antibodies were detected using horseradish peroxidase (HRP)-conjugated goat anti-human IgG detection antibody (Southern Biotech, Birmingham, AL, USA) at a 1:4,000 dilution and incubated for 90 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Then samples were washed three times with 0.1% Triton X-100 in PBS for 5 minutes and were incubated 30 minutes at RT with the following secondary antibody diluted 1:200: goat anti-rabbit Alexa Fluor 488 (Southern Biotech 4050-30), goat anti-mouse Alexa Fluor 488 (Southern Biotech 1030-30 ...
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bioRxiv - Immunology 2021Quote: ... Unbound antibodies were washed away with PBST and bound antibodies detected with 0.5 μg/mL HRP-conjugated goat anti-human constant kappa (2060-05, Southern Biotech, Birmingham, AL, USA) diluted in PBSTB ...
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bioRxiv - Microbiology 2021Quote: ... samples were further statically incubated for 1 h at 4°C with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech, 1:300) and 6 μM Syto9 (Live/Dead BacLight Bacterial Viability Kit ...
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bioRxiv - Bioengineering 2021Quote: ... The slides were subsequently incubated at room temperature with secondary antibodies: goat anti-rabbit Ig (1:100 dilution for 15 minutes, 4010-05, Southern Biotech, Birmingham, AL) for CD4 and goat anti-rabbit (1:50 dilution for 30 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... Antibodies bound to the thymocytes were detected by Cy5-conjugated goat anti-mouse IgG (1:250 dilution, 1030-15, Southern Biotech, Birmingham, AL) and Alexa Fluor 488-conjugated goat anti-mouse IgM (1:250 dilution ...
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bioRxiv - Immunology 2020Quote: ... with excess of PBS-T before being incubated for one hour at RT with an alkaline phosphatase-conjugated goat anti-human IgG (Southern Biotech, Birmingham, AL), diluted 3000 × in PBS-T ...
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bioRxiv - Immunology 2021Quote: ... IgM or IgA were detected by adding HRP-conjugated goat anti-mouse IgG (4 μg/ml; Southern Biotech, Cat#1030-05; RRID: AB_2619742), goat anti-mouse IgM (4 μg/ml ...
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bioRxiv - Microbiology 2021Quote: ... Goat anti-rabbit IgG-HRP (Cat No. 4030-05) and Goat anti-mouse IgG-HRP (Cat No. 1030-05) was from Southern Biotech (Alabama, USA). Goat anti-rabbit IgG Alexa fluor 488 (Cat no ...
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bioRxiv - Immunology 2022Quote: ... and the cells were stained with 50 μL/well of a 1:1,000 dilution of goat anti-human IgG-PE (Southern Biotech, cat. # 2040-09) secondary antibodies for 30 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... and stained with a 1:1,000 solution of goat anti-human IgG antibodies conjugated to phycoerythrin (PE) (Southern Biotech, Cat# 2040-09; RRID:AB_2795648) at 4°C for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... Goat anti-rabbit IgG-HRP (Cat No. 4030-05) and Goat anti-mouse IgG- HRP (Cat No. 1030-05) was from Southern Biotech (Alabama, USA). U0126 (Cat no ...
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bioRxiv - Microbiology 2022Quote: ... PrPSc on membranes was detected using mAb PRC5 at a dilution of 1:5000 followed by alkaline phosphatase conjugated goat anti mouse IgG (Southern Biotech, 1030-04) at a dilution of 1:5000 ...
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bioRxiv - Immunology 2022Quote: ... cells were washed twice with FACS buffer and incubated for 30 min at 4°C with 1:1000 diluted PE-conjugated goat F(ab)’2 anti-human IgG (Southern Biotech 2042-09) in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... 50 µm mouse brain sections were rinsed in PBS for 5 minutes and blocked with PBS/5% normal goat serum (Southern Biotech, 0060-01) for 2 hours at room temperature ...
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bioRxiv - Immunology 2023Quote: ... the plates were incubated with HRP-conjugated goat anti-human IgG and IgA secondary Abs (Southern Biotech, IgG: 2044-05, IgA: 2053-05) and developed by the TMB substrate (ThermoFisher) ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed and incubated with either AP-conjugated goat anti-mouse IgG or IgM antibody (SouthernBiotech; cat # 1030-04, 1020-04) (Supplementary Table 8) ...
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bioRxiv - Immunology 2023Quote: ... and the development of ADA in plasma samples were detected using a goat anti-human lambda IgG HRP-conjugated secondary antibody at 1:4000 dilution (Southern Biotech, Birmingham, AL). The plasma ADA levels were reported as endpoint titer ...
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bioRxiv - Microbiology 2024Quote: ... Plates were washed five times with PBS before adding 100 uL of HRP-conjugated goat anti-mouse IgG1 (Southern Biotech, Birmingham, AL, USA) diluted 1:4000 in blocking buffer and were then incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... They were then washed with PBS and incubated for 1 h at room temperature with the appropriate secondary antibody: goat anti-Rabbit IgG-HRP (Southern Biotech, #4030-05) or goat anti-Mouse IgG-HRP (Southern Biotech ...
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bioRxiv - Genetics 2021Quote: ... Wells were washed as described and biotin conjugated goat anti-human IgG (Jackson AffiniPure #109-065-098; 1:30,000 or Southern Biotech 2049-08; 1:1,000) in PBS/1.0% BSA detection antibody was added to the wells at 100ul/well and incubated for 2 hours at room temperature with shaking ...
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bioRxiv - Immunology 2020Quote: ... Horse spleen ferritin-specific IgA levels in the supernatant of the faecal homogenates was determined by enzyme-linked immunosorbent assay in plates coated with horse spleen ferritin followed by detection with horseradish peroxidase-conjugated goat anti-mouse IgA (Southern Biotech, Birmingham, AL, USA) using optEIA (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... 96-well Immulon 4 HBX plates were coated overnight at 4°C with 5 mg/ml polyclonal goat-anti mouse κ (Southern Biotech; 1050-01). Nonspecific binding was blocked with 1% bovine serum albumin in PBS and samples were incubated at 37°C ...
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bioRxiv - Immunology 2020Quote: ... Plates were then incubated with a 1:1000 dilution of mouse serum raised against ZE3-6H followed by goat anti-mouse IgG HRP conjugate (Southern Biotech, Birmingham, AL, USA) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Paraffin sections of kidneys were used to stain for collagen type IV using a goat polyclonal collagen IV antibody (Southern Biotech, Birmingham, AL, USA) (Thallas-Bonke et al. ...
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bioRxiv - Microbiology 2023Quote: ... Pellet was then resuspended in 100 µL ice-cold PBS containing 4 µg of FITC-coupled goat anti-mouse IgA antibody (SouthernBiotech, Imtec Diagnostic, #1040-02). The ratio of 4 µg of the respective antibody to stain 109 bacteria was previously evaluated to be the maximum amount of antibody that can be used without resulting in unspecific staining of non-IgA coated bacteria by using fecal samples from Rag1−/− mice as non IgA-coated negative controls ...
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bioRxiv - Neuroscience 2023Quote: ... They were washed six times in washing buffer (PBS with 0.1% Triton X-100) for 20 min and blocked for 1 h in washing buffer containing 5% heat-inactivated goat serum (SouthernBiotech, Cat. No. 0060-01), followed by incubation with the primary antibodies (rabbit anti–GFP ...
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bioRxiv - Immunology 2021Quote: ... FO B cells and MZ B cells were cultured in RPMI medium + Pen/Strep + L-glutamine + 10% FBS and stimulated with polyclonal goat anti-mouse IgM (Southern Biotech; 0.6-150 ug/mL), or equimolar concentrations of the Fab’2 fragments (Southern Biotech ...
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bioRxiv - Microbiology 2021Quote: For the detection of antibody titers in sera and fecal supernatants 96-well plates were coated with goat anti-human Ig (H+L chain) antibody (Southern Biotech, Cat. No. 2010-01) or goat anti-human IgA Fab (Southern Biotech ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed twice with Fc stain buffer and stained with Alexa Fluor 647 goat anti-human IgG (Southern Biotech [Birmingham, AL], cat. 2016-31) in FACS buffer for 30 minutes at 4°C followed by 2 wash steps with FC stain buffer and resuspended in Fluorofix (BioLegend ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated at RT for 1 h with the following HRP-conjugated secondary antibodies: goat anti-rabbit IgG conjugated to HRP (Southern Biotech, 4010-05; 1:5000), and goat anti-mouse IgG conjugated to HRP (Southern Biotech ...
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bioRxiv - Cancer Biology 2019Quote: ApoScreen Annexin V-FITC kit from Southern Biotech was used and protocol was followed according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: To detect mouse Ig Nunc MaxiSorp ELISA plates were coated for 1h at room temperature with goat anti-mouse Ig (H+L) (4 μg/ml; Southern Biotech, Cat#1010-01; RRID: AB_2794121). Citrobacter rodentium-specific Ig was quantified by coating Nunc MaxiSorp ELISA plates with 108 bacteria/well heat-inactivated at 60°C for 1 h ...
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bioRxiv - Immunology 2023Quote: ... Membranes were washed with TBST (1x, 5 min., RT) and incubated with goat anti-rabbit IgG- HRP (Southern Biotech #1021-05, 10 mL, 1:5,000, 1 h, RT). Membranes were washed with TBST (3x ...
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bioRxiv - Immunology 2022Quote: ... Antibody isotyping kit was purchased from Southern Biotech (Cat#5300-05). FITC-conjugated streptavidin was purchased from BD Biosciences (Cat#554060) ...