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Citations for SouthernBiotech :
601 - 650 of 683 citations for T Cell Surface Glycoprotein CD3 Gamma Chain CD3G Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Sections were washed in PBS before incubation with secondary antibodies (Table 3) for 1 hr at room temperature followed by mounting with DAPI containing fluoromount (Southern Biotech, Birmingham, AL, USA). Images were captured with an EVOS FL imaging system (ThermoFisher Scientific) ...
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bioRxiv - Physiology 2023Quote: ... The membranes were then washed 3x in TBST before incubation for 1 hour at room temperature with peroxidase-conjugated secondary antibodies in TBST at 1:10,000 (Southern Biotech, #1031-05, #4050-05). Antibody binding was detected using an enhanced chemiluminescence HRP substrate detection kit (Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... Paraffin sections of kidneys were used to stain for collagen type IV using a goat polyclonal collagen IV antibody (Southern Biotech, Birmingham, AL, USA) (Thallas-Bonke et al. ...
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bioRxiv - Microbiology 2023Quote: ... Pellet was then resuspended in 100 µL ice-cold PBS containing 4 µg of FITC-coupled goat anti-mouse IgA antibody (SouthernBiotech, Imtec Diagnostic, #1040-02). The ratio of 4 µg of the respective antibody to stain 109 bacteria was previously evaluated to be the maximum amount of antibody that can be used without resulting in unspecific staining of non-IgA coated bacteria by using fecal samples from Rag1−/− mice as non IgA-coated negative controls ...
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bioRxiv - Biophysics 2021Quote: ... Cell lines were tested for mycoplasma contamination via the Mycoplasma Detection Kit (Southern Biotech). Transient transfections were performed with Lipofectamine 2000 (Life Technologies) ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated with goat anti-human IgG Fc-HRP (Southern Biotech, 2014-05) diluted at 1:1000 in Perm Wash for 1.5 h at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were mounted with DAPI Fluoromount-G media with DAPI nuclear stain (Southern Biotech). Slides were viewed with Olympus microscopy.
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bioRxiv - Neuroscience 2021Quote: ... We then incubated cells with goat-anti-rabbit-APC (Southern Biotech, Cat# 4050-11S), mouse anti-human monoclonal antibodies against p75/NGFR (Becton-Dickinson ...
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bioRxiv - Neuroscience 2021Quote: ... Cell nuclei were stained with DAPI and mounted with Fluoromount-G mounting medium (SouthernBiotech). For β-gal staining ...
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bioRxiv - Neuroscience 2022Quote: ... cells were mounted with DAPI Fluoromount-G® Mounting Media (Southern Biotech, 0100-20). All images were acquired using a confocal laser scanning microscope (LSM 800 with Airyscan ...
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bioRxiv - Biochemistry 2023Quote: ... in PBS for 5 min and mounted the cells with Fluoromount-G (Southern Biotech). For negative control ...
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bioRxiv - Neuroscience 2024Quote: ... Stained cells were cover-slipped in DAPI Fluoromount G mounting medium (SouthernBiotech, 0100-20), allowed to dry ...
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bioRxiv - Cancer Biology 2024Quote: ... Sections or cells were counterstained with DAPI and mounted with Fluoromount-G (Southern Biotech) before being visualized using a Nikon Eclipse Ti microscope ...
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bioRxiv - Immunology 2020Quote: ... or F(ab)2 polyclonal goat anti-human IgM (Jackson Immuno Research) as capture antibody and detection with HRP-conjugated mouse μ-specific anti-human IgM (Southern Biotech, clone SA-DA4).
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bioRxiv - Cell Biology 2023Quote: ... and incubated at RT for 1 h with the following HRP-conjugated secondary antibodies: goat anti-rabbit IgG conjugated to HRP (Southern Biotech, 4010-05; 1:5000), and goat anti-mouse IgG conjugated to HRP (Southern Biotech ...
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bioRxiv - Microbiology 2024Quote: ... Cells were incubated cell side down in 100 µL of Stellaris RNA FISH Hybridization Buffer (BioSearch) containing M1 vRNA probes (1/100) and the primary antibodies of interest (NP, Southern Biotech, 10770-01, 1/100) and incubated in a humid chamber at 37°C in the dark for 16 h ...
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bioRxiv - Cancer Biology 2021Quote: ... No mycoplasma contamination was detected in this cell line throughout the experiments (SouthernBiotech #13100-01). XPA expression plasmids contain full-length human XPA (NM_000380 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed then coincubated with PE-conjugated mouse anti-human IgG Fc (Southern Biotech) diluted to 1:200 for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Fixed cells were mounted on a glass slide with Fluoromount-G mounting media (Southern Biotech). Images were acquired using confocal LSM780 (Zeiss ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed and incubated at 4°C with goat anti-mouse Ig-PE (SouthernBiotech). Cells were washed and mean fluorescence intensity (MFI ...
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bioRxiv - Microbiology 2023Quote: ... cells were labeled with 2 μg/ml PE-conjugated goat anti-human IgG (Southern Biotech) for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed with 1% BSA in PBS twice before incubating with secondary anti-rat IgG antibody conjugated with APC (1:500; Southern Biotech, AL, USA, Catalog no. 3010-11L). Cells were analyzed in BD FACSVerse™ flow cytometer (BD Biosciences ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were then washed x3 with HBSS and slides were mounted using Fluoromount-G (Southern Biotech). For intracellular immunostaining cells were washed x1 with PBS ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed and incubated for 5 min in ice with streptavidin (60 μg/ml, SouthernBiotech) for cross-linking and incubated at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Fluoromount-G used to mount cells for imaging was obtained from Southern Biotech (Cat# 0100-01). AZD1152 (AURKB inhibitor Cat# SML0268 ...
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bioRxiv - Bioengineering 2020Quote: ... cells were washed with PBS three times followed by treatment with DAPI Fluoromount-G (Southern Biotech). Cells were imaged with a Leica SP8 3×STED superresolution microscope.
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bioRxiv - Genomics 2023Quote: ... Cells were checked for mycoplasma contamination using the Mycoplasma detection kit (Southern Biotech, catalog #13100-01) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then washed with PBS and mounted on slides with Fluoromount G (SouthernBiotech #0100-01) for imaging and quantification using the CellInsight CX7 LED Pro High-Content Screening Platform (ThermoFisher Scientific HCSDCX7LEDPRO).
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bioRxiv - Cell Biology 2024Quote: ... Cells were regularly validated as mycoplasma free by a PCR-based mycoplasma detection kit (Southern Biotech).
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed again 3x with PBS before allowing to dry and mounting with Fluoromount-G (SouthernBioTech).
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bioRxiv - Immunology 2022Quote: ... Cells were further incubated with biotinylated goat anti-rabbit IgG (H+L) and HRP-streptavidin (Southern Biotech). The reactions were developed with o-Phenylenediamine di-hydrochloridec (OPD ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were collected and stained on ice with Alexa-fluor 488-coupled anti-IgA Ab (Southern Biotech) and 7AAD (Roche ...
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bioRxiv - Cell Biology 2020Quote: The apoptosis level of PC12 cells was determined by ApoScreen Annexin V kit (SouthernBiotech, Birmingham, AL, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed three times with 1x PBST and mounted with DAPI Fluoromount-G® (SouthernBiotech, AL).
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bioRxiv - Immunology 2023Quote: ... Cells were then activated with 20 µg/mL of F(ab’)2 anti-human IgM (Southern Biotech) or left resting ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then washed and mounted on the slide glass using fluromount-G® (SouthernBiotech, 011-01).
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bioRxiv - Molecular Biology 2024Quote: ... Glass slides with the cells were washed with PBS and mounted with DAPI Fluoromount-G (SouthernBiotech, USA). LSM 510 META inverted confocal microscope at a 40× magnification (Carl Zeiss Micro Imaging ...
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bioRxiv - Microbiology 2024Quote: ... Cells were routinely confirmed to be mycoplasma free by conducting PCRs using a mycoplasma detection kit (SouthernBiotech).
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bioRxiv - Microbiology 2019Quote: ... This cell line was routinely tested for mycoplasma contamination using a Mycoplasma Detection Kit (SouthernBiotech, Cat# 13100-01).
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bioRxiv - Cell Biology 2021Quote: ... and coverslips were mounted on top of the cells using Fluoromount-G mounting solution containing DAPI (Southern Biotech). Single slice images were taken on a Zeiss 880 microscope using the confocal settings.
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bioRxiv - Microbiology 2020Quote: ... Cells were washed a further three times before mounting with Fluoromount G with DAPI (Cambridge Bioscience, Southern Biotech). Imaging was performed with an Axioscope 2 fluorescence microscope (Zeiss ...
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bioRxiv - Neuroscience 2021Quote: ... cell nuclei were stained using DAPI during the first round of HCR-FISH staining (DAPI Fluoromount-G; SouthernBiotech).
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bioRxiv - Immunology 2023Quote: ... Cells were either left unstimulated or 20 µg/mL of F(ab’)2 anti-human IgM (Southern Biotech) was added directly to the well ...
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bioRxiv - Immunology 2023Quote: ... cells were either left unstimulated or 20 µg/mL of F(ab’)2 anti-human IgM (Southern Biotech) was added directly to the well ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were routinely screened and confirmed negative for mycoplasma contamination at least every three months (SouthernBiotech #13100-01).
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bioRxiv - Microbiology 2023Quote: ... Cells were washed with PBS and incubated for 1 h with a secondary goat anti-mouse FITC (SouthernBiotech) at a 20 μg/mL concentration and phalloidin (7 units ...
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bioRxiv - Immunology 2023Quote: ... Cells were either left unstimulated or 20 µg/mL of F(ab’)2 anti-human IgM (Southern Biotech) was added directly to the well ...
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bioRxiv - Immunology 2023Quote: ... cover slips with stained cells were mounted with DAPI Fluoromount-G (Southern Biotech, AL, USA, Cat. 0100-20) on microscope slides and analyzed by an AxioObserver Z.1 Microscope with 40x or 63x objectives (Zeiss ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed 3 times with PBST and mounted on slides with Fluoromount-G (SouthernBiotech #0100-01) or ProLong™ Glass Antifade Mountant (Invitrogen P36984) ...
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bioRxiv - Immunology 2024Quote: ... Human B cell stimulation used 10ng/mL Goat F(ab’)2 Anti-human IgM (Southern Biotech #2022-01), 2ug/mL of recombinant human sCD40 (invitrogen PHP0025) ...