Labshake search
Citations for SouthernBiotech :
601 - 633 of 633 citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... IgG3 was measured following 6 washes and a 1-h incubation with 100 μL/well 1:5,000 mouse anti-human biotin-conjugated IgG3 hinge (SouthernBiotech, Birmingham, Alabama, USA) then 6 washes and 1-h incubation with 100 μL/well 1:40,000 HRP-conjugated streptavidin (Jackson ImmunoResearch Labs) ...
-
bioRxiv - Immunology 2023Quote: ... plates were washed 3 times and incubated with 50 μl of either 0.25 μg/ml biotinylated anti-mouse IgG1 (Southern Biotech, 1070-08), or biotinylated anti-mouse IgG (H+L ...
-
bioRxiv - Immunology 2023Quote: ... Plates were then incubated for 90min at RT with goat anti-mouse IgG conjugated to horseradish peroxidase (HRP) (Southern Biotech, #1030-05) at 1:5000 dilution in blocking solution ...
-
bioRxiv - Bioengineering 2023Quote: ... followed by 1 hr incubation with horseradish peroxidase-labeled goat anti-mouse secondary antibody (GAM-HRP, SouthernBiotech Cat#1010-05, RRID: AB_2728714). After washing ...
-
bioRxiv - Immunology 2024Quote: ... Cells were incubated with neoepitope-targeting mAbs and subsequently detected with a mouse anti-human IgG Fc-PE secondary antibody (SouthernBiotech 9040-09). Detection of the rabbit pAb cocktail was conducted with a goat anti-rabbit IgG (H+L ...
-
bioRxiv - Immunology 2024Quote: ... Plates were then washed 3x with wash buffer and incubated with a 1:4,000 dilution (in EZ block) of Goat Anti-Mouse IgG Fc-HRP secondary (Southern Biotech, 1033-05) for 1 hr at RT ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells were then washed with PBS-BSA and stained with secondary antibodies (5 μM Alexa Fluor® 488 anti-mouse (SouthernBiotech; 1031-30) and 10 μM Alexa Fluor® 647 anti-human (SouthernBiotech ...
-
bioRxiv - Bioengineering 2021Quote: ... Antibodies bound to the thymocytes were detected by Cy5-conjugated goat anti-mouse IgG (1:250 dilution, 1030-15, Southern Biotech, Birmingham, AL) and Alexa Fluor 488-conjugated goat anti-mouse IgM (1:250 dilution ...
-
bioRxiv - Immunology 2021Quote: ... For detection of IgG subclasses all serum samples were diluted to 1 total IgG EU and incubated at 37 °C for 1 h prior to detection with Alkaline Phosphatase conjugated anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam) incubated for 1 h at 37 °C ...
-
bioRxiv - Immunology 2021Quote: ... plates were washed with PBS-T and HRP-conjugated goat anti-mouse IgG or goat anti-human IgG (Southern Biotech, Birmingham, AL, USA) added for 1 hour ...
-
bioRxiv - Immunology 2020Quote: ... followed by two rounds of fluorescence-activated cell sorting (FACS) of cells stained with FITC-Conjugated rat anti-mouse IgG1 (Southern Biotech 1144-02), and rounds of soft-agar cloning in SeaPlaque agarose ...
-
bioRxiv - Microbiology 2020Quote: ... plates were washed with PBS-T and HRP-conjugated goat anti-mouse IgG or goat anti-human IgG (Southern Biotech, Birmingham, AL, USA) added for 1 hour ...
-
bioRxiv - Immunology 2021Quote: ... IgM or IgA were detected by adding HRP-conjugated goat anti-mouse IgG (4 μg/ml; Southern Biotech, Cat#1030-05; RRID: AB_2619742), goat anti-mouse IgM (4 μg/ml ...
-
bioRxiv - Microbiology 2021Quote: ... Goat anti-rabbit IgG-HRP (Cat No. 4030-05) and Goat anti-mouse IgG-HRP (Cat No. 1030-05) was from Southern Biotech (Alabama, USA). Goat anti-rabbit IgG Alexa fluor 488 (Cat no ...
-
bioRxiv - Immunology 2020Quote: ... all serum samples were diluted to 1 total IgG ELISA unit and then detected with anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam). The results of the IgG subclass ELISA are presented using OD values instead of the ELISA units used for the total IgG ELISA ...
-
bioRxiv - Microbiology 2022Quote: ... Goat anti-rabbit IgG-HRP (Cat No. 4030-05) and Goat anti-mouse IgG- HRP (Cat No. 1030-05) was from Southern Biotech (Alabama, USA). U0126 (Cat no ...
-
bioRxiv - Microbiology 2022Quote: ... PrPSc on membranes was detected using mAb PRC5 at a dilution of 1:5000 followed by alkaline phosphatase conjugated goat anti mouse IgG (Southern Biotech, 1030-04) at a dilution of 1:5000 ...
-
bioRxiv - Microbiology 2024Quote: ... Plates were washed five times with PBS before adding 100 uL of HRP-conjugated goat anti-mouse IgG1 (Southern Biotech, Birmingham, AL, USA) diluted 1:4000 in blocking buffer and were then incubated overnight at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... 50 µm mouse brain sections were rinsed in PBS for 5 minutes and blocked with PBS/5% normal goat serum (Southern Biotech, 0060-01) for 2 hours at room temperature ...
-
bioRxiv - Immunology 2023Quote: ... The plates were washed and incubated with either AP-conjugated goat anti-mouse IgG or IgM antibody (SouthernBiotech; cat # 1030-04, 1020-04) (Supplementary Table 8) ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The secondary antibodies were goat anti-rabbit (#4010-05, 1:5000) and anti-mouse (#1030-05, 1:5000, both from Southern Biotech, Birmingham AL) coupled to horseradish peroxidase ...
-
bioRxiv - Immunology 2024Quote: ... Antibody secreting cells were detected with alkaline phosphatase (AP)-conjugated antibodies goat anti-mouse IgM (0.5 µg/mL; SouthernBiotech Cat#1020-04, RRID:AB_2794200) or goat anti-mouse IgG (0.5 µg/mL ...
-
bioRxiv - Immunology 2024Quote: ... membranes were incubated with indicated primary antibodies overnight at 4 °C and with horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (SouthernBiotech, Birmingham, AL, USA) for 2 hours at room temperature ...
-
bioRxiv - Immunology 2024Quote: Fifty microliters of appropriately diluted serum samples were added to 96-well plates precoated with anti-mouse Igκ and Igλ antibodies (2 mg/ml; Southern Biotech, Birmingham, AL) in 0.1 M carbonate buffer (pH 9.0) ...
-
bioRxiv - Immunology 2022Quote: ... Plates were washed with water three times before applying 25 μl of secondary antibody (goat anti-human IgG Fc-Southern Biotech, 2048-04; goat anti-mouse IgG Fc-Southern Biotech, 1033-04) at a dilution of 1:4,000 in blocking buffer ...
-
bioRxiv - Immunology 2020Quote: ... Horse spleen ferritin-specific IgA levels in the supernatant of the faecal homogenates was determined by enzyme-linked immunosorbent assay in plates coated with horse spleen ferritin followed by detection with horseradish peroxidase-conjugated goat anti-mouse IgA (Southern Biotech, Birmingham, AL, USA) using optEIA (BD Biosciences ...
-
bioRxiv - Immunology 2020Quote: ... washed 6 times and then incubated with 1: 6000 dilution of horseradish peroxidase (HRP) conjugated anti-mouse IgG secondary antibody (Southern Biotech; Birmingham, AL, USA) for 1 h at RT ...
-
bioRxiv - Microbiology 2023Quote: ... Pellet was then resuspended in 100 µL ice-cold PBS containing 4 µg of FITC-coupled goat anti-mouse IgA antibody (SouthernBiotech, Imtec Diagnostic, #1040-02). The ratio of 4 µg of the respective antibody to stain 109 bacteria was previously evaluated to be the maximum amount of antibody that can be used without resulting in unspecific staining of non-IgA coated bacteria by using fecal samples from Rag1−/− mice as non IgA-coated negative controls ...
-
bioRxiv - Immunology 2024Quote: ... The plates were washed before the addition of goat anti-mouse IgG secondary antibody conjugated with alkaline phosphatase (AP) (Southern Biotech, Birmingham, AL, USA) for 2 hr ...
-
bioRxiv - Immunology 2021Quote: ... FO B cells and MZ B cells were cultured in RPMI medium + Pen/Strep + L-glutamine + 10% FBS and stimulated with polyclonal goat anti-mouse IgM (Southern Biotech; 0.6-150 ug/mL), or equimolar concentrations of the Fab’2 fragments (Southern Biotech ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Plates were incubated with serially diluted serum or gut content (50 μL, PBS 1% BSA, wt/vol, 2 h, RT) and standard mouse IgA (Southern Biotech #0106-01, prepared in duplicates) and washed (5x ...
-
bioRxiv - Immunology 2021Quote: To detect mouse Ig Nunc MaxiSorp ELISA plates were coated for 1h at room temperature with goat anti-mouse Ig (H+L) (4 μg/ml; Southern Biotech, Cat#1010-01; RRID: AB_2794121). Citrobacter rodentium-specific Ig was quantified by coating Nunc MaxiSorp ELISA plates with 108 bacteria/well heat-inactivated at 60°C for 1 h ...
-
bioRxiv - Cell Biology 2024Quote: ... Plates were washed three times with wash buffer and incubated with 100 µL per well of HRP-conjugated α-mouse IgG1 (Southern Biotech, 1070-05, 1:5000 in 1 % BSA in PBS) or HRP-conjugated α-mouse IgM (Southern Biotech ...