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Citations for SouthernBiotech :
601 - 650 of 699 citations for Mouse Vang Like Protein 2 VANGL2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... was added to the wells for 1h followed by washing and incubation with horseradish peroxidase-linked goat anti-mouse IgG secondary antibody (Southern Biotech). The avidity index was calculated as ...
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bioRxiv - Microbiology 2023Quote: ... the wells were washed with the washing buffer twice and incubated with horseradish peroxidase (HRP)-conjugated mouse anti-monkey IgG (Southern Biotech) at room temperature for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... and incubated with pleural lavage fluid overnight at 4°C prior to detection of bound IgM with a goat anti-mouse IgM-HRP (1020-05 Southern Biotech) for 1h at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... then washed and secondary antibody added at 1:2000 or 1:4000 dilution in blocking buffer (100□μl/well) using either anti-mouse IgG-HRP (Catalog #1030-05, Southern Biotech), or anti-mouse IgA-biotin (Catalog #1040-80 ...
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bioRxiv - Cell Biology 2023Quote: ... Labeling of primary antibodies was visualized with anti-mouse PE and anti-rat PE (both Southern Biotech, USA, 1:1000 diluted). Actin in living cells was visualized with SiR actin (Spirochrome ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies conjugated with HRP used in the study are: Goat Anti-mouse IgG1-HRP (1:10000, Southern Biotech, 1071-05), Goat Anti-rabbit IgG-HRP (1:10000 ...
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bioRxiv - Immunology 2022Quote: ... NP-specific IgM and IgG3 antibodies were detected using biotinylated anti-mouse IgM- or IgG3-specific immunoglobulins followed by streptavidin-HRP (Southern Biotech) and developed with SIGMAFAST OPD tablets (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... Precomplexation of the anti-sialyl-LewisX antibody was performed with a 1:50 dilution of goat anti-mouse IgM-HRP (#1021-05, Southern Biotech) and 0.65 µg donkey anti-goat Alexa Fluor 555 (A21432 ...
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bioRxiv - Microbiology 2023Quote: ... 1×109 bacteria were washed with 1 ml ice-cold PBS and stained for 30 min at 4°C with 4 µg of FITC-conjugated anti-mouse IgA antibody (Southern Biotech) in 100 µl of staining buffer ...
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bioRxiv - Immunology 2023Quote: ... Bound IL-21 was subsequently detected with mouse anti-human IL-21-biotin (clone 2B2-G20, eBioscience) followed by alkaline phosphatase-conjugated streptavidin (Southern Biotech). The signals were compared to a standard curve generated with recombinant human IL-21 (R&D Systems).
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bioRxiv - Immunology 2023Quote: ... The plates were washed and incubated with a peroxidase-conjugated goat anti-mouse anti-IgM or anti-IgG antibody (Southern Biotech). After washing ...
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bioRxiv - Immunology 2024Quote: ... The secondary antibodies used for western blot analysis were goat anti-rabbit conjugated to horseradish peroxidase-conjugated (HRP) (#4050-05, diluted at 1:1000) and anti-mouse-HRP (#1031-05, diluted at 1:1000) from SouthernBiotech (USA).
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies including anti-mouse immunoglobulin G (IgG; 1010-05) and anti-rabbit IgG (catalog no. 4010-05) were obtained from Southern biotech. Primary antibodies were used at a 1:1000 dilution (unless otherwise stated) ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were washed with TBS-T 0.1% three times and incubated for one hr at room temperature with goat anti-mouse Ig horseradish peroxidase (HRP)-conjugated antibodies (Southern Biotech, anti-IgM secondary 1:1000 (cat# 1020-05) ...
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bioRxiv - Cell Biology 2023Quote: ... The presence of the sLAP proteins was verified by western blotting according to standard protocols and by staining with anti-human HRP antibodies (Southern Biotech, USA). Concentration of sLAP proteins was quantified by dot blotting and using purified human Fc-IgG (SigmaAldrich ...
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The antibiotic bedaquiline activates host macrophage innate immune resistance to bacterial infectionbioRxiv - Immunology 2019Quote: ... coverslips were set in Fluoromount G medium containing 1 µg/ml 4′,6-diamidino-2-phenylindole (DAPI) (SouthernBiotech, Birmingham, Alabama) on microscope slides ...
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bioRxiv - Immunology 2021Quote: ... Isolated neutrophils were incubated for 30 minutes at room temperature with 20 μg/ml control F(ab’)2 (Southern Biotech) or mouse-anti-SIRL-1 F(ab’)2 (own production) ...
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bioRxiv - Immunology 2023Quote: Biotinylated Goat F(ab)2 anti-human isotype antibodies (anti-human IgM, SouthernBiotech, Cat # 2022-01; anti-human IgA, SouthernBiotech, Cat # 2052-01 ...
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bioRxiv - Neuroscience 2023Quote: ... Coverslips were washed with PBS and mounted with Fluoromount-G with DAPI (4’,6’-diamidino-2-phenylindole dihydrochloride) (cat. no. 0100-20, SouthernBiotech) for nuclear staining ...
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bioRxiv - Immunology 2022Quote: ... Antibody isotyping kit was purchased from Southern Biotech (Cat#5300-05). FITC-conjugated streptavidin was purchased from BD Biosciences (Cat#554060) ...
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bioRxiv - Immunology 2019Quote: ... Murine antibodies were detected using alkaline phosphatase conjugated goat anti-mouse IgG or IgA (Southern Biotech, Cat #1030-04 and 1040-04). Rabbit antibodies were detected using alkaline phosphatase conjugated goat anti-rabbit IgG (Southern Biotech ...
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bioRxiv - Microbiology 2021Quote: ... The primary antibodies were detected by secondary antibody anti-mouse or anti-rabbit conjugated to horseradish peroxidase (Southern Biotech, 1:10,000 dilution) for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... then incubated in fresh dilution solution with secondary antibody (goat anti-mouse IgG2a AlexaFluor 488, 1:250, Southern Biotech, cat# 1080-30) overnight at 4°C with gentle shaking ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed with 0.05% Tween-20 in PBS and further incubated with HRP-conjugated goat antibodies directed against mouse IgG (Southern Biotech, Birmingham, AL) for 1 hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed twice in FACS buffer and stained with 50 μl/well of 1:200 dilution of R-phycoerythrin (PE)-conjugated mouse anti-human IgG Fc antibody (SouthernBiotech #9040-09) and 1:1000 dilution of Zombie-NIR viability dye (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... plates were subsequently incubated with 0.1 µg/mL HRP-labeled goat anti-mouse κ light chain Ab (Southern Biotech, Birmingham, AL 35260), diluted in assay buffer for 1 hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... except constructs were bound directly to the plate and detected with goat anti-mouse IgG HRP conjugate (Southern Biotech, Birmingham, AL, USA).
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bioRxiv - Immunology 2021Quote: ... The secondary antibody used was horseradish-peroxidase (HRP)-conjugated goat polyclonal antibody against mouse IgG1 (1070-05; Southern Biotech, Cambridge, United Kingdom), HRP-conjugated goat polyclonal antibody against rabbit IgG (4050-05 ...
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bioRxiv - Immunology 2020Quote: ... plates were washed with PBS-T and HRP-conjugated goat anti-mouse IgG or anti-rat IgG (Southern Biotech, Birmingham, AL, USA) added for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... the cells were stained with 50μl/well of 1:200 diluted R-phycoerythrin (PE)-conjugated mouse anti-human IgG Fc antibody (SouthernBiotech cat.# 9040-09) and 1:1000 dilution of Zombie-NIR viability dye (BioLegend cat.# 423105 ...
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bioRxiv - Immunology 2023Quote: ... IgG3 was measured following 6 washes and a 1-h incubation with 100 μL/well 1:5,000 mouse anti-human biotin-conjugated IgG3 hinge (SouthernBiotech, Birmingham, Alabama, USA) then 6 washes and 1-h incubation with 100 μL/well 1:40,000 HRP-conjugated streptavidin (Jackson ImmunoResearch Labs) ...
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bioRxiv - Immunology 2023Quote: ... plates were washed 3 times and incubated with 50 μl of either 0.25 μg/ml biotinylated anti-mouse IgG1 (Southern Biotech, 1070-08), or biotinylated anti-mouse IgG (H+L ...
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bioRxiv - Immunology 2023Quote: ... Plates were then incubated for 90min at RT with goat anti-mouse IgG conjugated to horseradish peroxidase (HRP) (Southern Biotech, #1030-05) at 1:5000 dilution in blocking solution ...
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bioRxiv - Bioengineering 2023Quote: ... followed by 1 hr incubation with horseradish peroxidase-labeled goat anti-mouse secondary antibody (GAM-HRP, SouthernBiotech Cat#1010-05, RRID: AB_2728714). After washing ...
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bioRxiv - Immunology 2024Quote: ... plates were washed four times and 25 µl of a 1:500 dilution of the respective detection antibody (goat anti-mouse IgG(H+L)-alkaline phosphatase (AP) conjugate (Southern Biotech/Biozol) or goat anti-mouse IgG1-AP (Southern Biotech/Biozol ...
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bioRxiv - Microbiology 2020Quote: ... IgG-bound DNP-beads were next incubated with 1 µg/mL Alexa Fluor647-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech) in PBS-TH.
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bioRxiv - Microbiology 2020Quote: ... Antibody binding was detected using directly labeled IgG or Alexa Fluor647-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech). Samples were measured using flow cytometry (BD FACSVerse ...
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in calcium-containing HBSS and incubated at room temperature for 5 minutes before activation by anti-IgM F(ab′)2 (Southern Biotech) or VLPs ...
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bioRxiv - Developmental Biology 2022Quote: ... They were washed in blocking buffer 2X for 2 hours and imaged in situ or mounted on microscope slides using Fluoromount-G (Southern Biotech). Immunostaining Analysis ...
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bioRxiv - Microbiology 2022Quote: ... bacteria were incubated with a Goat F(ab’)2 anti-human Kappa-Alexa Fluor 647 (pre-diluted in PBS 0.1%BSA; 2.5 μg/mL, Southern Biotech #2062-31) to detect bound Fab fragments ...
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bioRxiv - Immunology 2019Quote: ... the membrane was re-blocked with 5% BSA in 1x TBST for 2 hours at room temperature before probing with 1:5000 dilution of anti-human IgG-HRP (Southern Biotech) in 5% BSA/1x TBST for 1 hour at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Fab fragments were detected with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech, 1:500). After washing ...
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bioRxiv - Neuroscience 2020Quote: ... Nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) with the use of DAPI Fluoromount-G (0100-20; Southern Biotech). Sections were also stained for β-galactosidase (β-gal ...
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bioRxiv - Immunology 2021Quote: ... B cells were then cultured for a further 2 days in RPMI (as described above) or supplemented with 500ng/ml Igκ (Southern Biotech). Where indicated the following reagents were added into in vitro cultures ...
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bioRxiv - Microbiology 2020Quote: ... Guts were washed 5 times in PBS-T and then mounted on slides in Flouromount plus 4’,6-diamidino-2-phenylindole nuclear stain (DAPI, Southern Biotech) and sealed with a cover slip ...
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bioRxiv - Cell Biology 2020Quote: ... localization of antibody labeling was detected after a 1 hr incubation with secondary antibody diluted in PBS containing 2% normal goat serum and mounting under a coverslip with DAPI Fluoromount-G (Southern Biotech). PPCs were visualized by fluorescent microscopy and their numbers were assessed qualitatively by visual examination ...
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bioRxiv - Biochemistry 2020Quote: ... The immunoreactivity was detected by incubation with Rabbit F(ab’)2 Anti-Goat IgG(H+L)-HRP (6020-05, Southern Biotech) diluted 3000 × in PBS-T.
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed again and the nuclei were stained with DAPI (4’,6-diamidino-2-phenylindole) before mounting with Fluoromount-G (Southern Biotech). Primary antibodies against histone H3 methylation on lysine 4 ...
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bioRxiv - Cancer Biology 2024Quote: ... permeabilized with 0.1% Triton X-100 (Fisher Biotech, Hampton, NH, USA), and blocked with PBS supplemented with goat serum (2%, v/v) (Southern biotech) and 0.1% Tween-20 (Thermo Fisher) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... cells were washed with ice cold PBS buffer and then stained with a PE-labelled anti-mouse IgG secondary antibody (Southern Biotech, Birmingham, AL) at 1 μg/ml concentration for 30 min on ice ...