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Citations for SouthernBiotech :
551 - 600 of 834 citations for Recombinant Mouse Frizzled Homolog 1 Drosophila His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and incubated with pleural lavage fluid overnight at 4°C prior to detection of bound IgM with a goat anti-mouse IgM-HRP (1020-05 Southern Biotech) for 1h at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... the wells were washed with the washing buffer twice and incubated with horseradish peroxidase (HRP)-conjugated mouse anti-monkey IgG (Southern Biotech) at room temperature for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... was added to the wells for 1h followed by washing and incubation with horseradish peroxidase-linked goat anti-mouse IgG secondary antibody (Southern Biotech). The avidity index was calculated as ...
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bioRxiv - Immunology 2022Quote: ... NP-specific IgM and IgG3 antibodies were detected using biotinylated anti-mouse IgM- or IgG3-specific immunoglobulins followed by streptavidin-HRP (Southern Biotech) and developed with SIGMAFAST OPD tablets (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... 1×109 bacteria were washed with 1 ml ice-cold PBS and stained for 30 min at 4°C with 4 µg of FITC-conjugated anti-mouse IgA antibody (Southern Biotech) in 100 µl of staining buffer ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed and incubated with a peroxidase-conjugated goat anti-mouse anti-IgM or anti-IgG antibody (Southern Biotech). After washing ...
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bioRxiv - Immunology 2023Quote: ... Bound IL-21 was subsequently detected with mouse anti-human IL-21-biotin (clone 2B2-G20, eBioscience) followed by alkaline phosphatase-conjugated streptavidin (Southern Biotech). The signals were compared to a standard curve generated with recombinant human IL-21 (R&D Systems).
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies including anti-mouse immunoglobulin G (IgG; 1010-05) and anti-rabbit IgG (catalog no. 4010-05) were obtained from Southern biotech. Primary antibodies were used at a 1:1000 dilution (unless otherwise stated) ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were washed with TBS-T 0.1% three times and incubated for one hr at room temperature with goat anti-mouse Ig horseradish peroxidase (HRP)-conjugated antibodies (Southern Biotech, anti-IgM secondary 1:1000 (cat# 1020-05) ...
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bioRxiv - Immunology 2024Quote: ... membranes were incubated with primary antibodies overnight at 4 °C and during 2 hours at room temperature with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit IgG (SouthernBiotech, USA). Membranes were then revealed using G:BOX Chemi XL1.4 Fluorescent & Chemiluminescent Imaging System (Syngene ...
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bioRxiv - Neuroscience 2023Quote: ... anti-sheep (Novus NBP1-73267) or a 1:1 mix of anti-rabbit (Southern Biotech; OB405005) and anti-rabbit (Cell Signaling ...
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bioRxiv - Immunology 2019Quote: ... Murine antibodies were detected using alkaline phosphatase conjugated goat anti-mouse IgG or IgA (Southern Biotech, Cat #1030-04 and 1040-04). Rabbit antibodies were detected using alkaline phosphatase conjugated goat anti-rabbit IgG (Southern Biotech ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed with 0.05% Tween-20 in PBS and further incubated with HRP-conjugated goat antibodies directed against mouse IgG (Southern Biotech, Birmingham, AL) for 1 hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... plates were subsequently incubated with 0.1 µg/mL HRP-labeled goat anti-mouse κ light chain Ab (Southern Biotech, Birmingham, AL 35260), diluted in assay buffer for 1 hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... except constructs were bound directly to the plate and detected with goat anti-mouse IgG HRP conjugate (Southern Biotech, Birmingham, AL, USA).
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bioRxiv - Immunology 2021Quote: ... The secondary antibody used was horseradish-peroxidase (HRP)-conjugated goat polyclonal antibody against mouse IgG1 (1070-05; Southern Biotech, Cambridge, United Kingdom), HRP-conjugated goat polyclonal antibody against rabbit IgG (4050-05 ...
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bioRxiv - Immunology 2020Quote: ... plates were washed with PBS-T and HRP-conjugated goat anti-mouse IgG or anti-rat IgG (Southern Biotech, Birmingham, AL, USA) added for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... plates were washed 3 times and incubated with 50 μl of either 0.25 μg/ml biotinylated anti-mouse IgG1 (Southern Biotech, 1070-08), or biotinylated anti-mouse IgG (H+L ...
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bioRxiv - Immunology 2023Quote: ... Plates were then incubated for 90min at RT with goat anti-mouse IgG conjugated to horseradish peroxidase (HRP) (Southern Biotech, #1030-05) at 1:5000 dilution in blocking solution ...
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bioRxiv - Neuroscience 2021Quote: ... Collagen IV (SouthernBiotech, Goat 1:100), Thbs1 (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... and 1/5,000 HRP-Steptavidin (100 μL/well) was added for 1 h (Southern Biotech, #7105-05). Plates were washed 4 times again with PBS-Tween 20 0.05 % ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... cells were washed with ice cold PBS buffer and then stained with a PE-labelled anti-mouse IgG secondary antibody (Southern Biotech, Birmingham, AL) at 1 μg/ml concentration for 30 min on ice ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were then washed with PBS-BSA and stained with secondary antibodies (5 μM Alexa Fluor® 488 anti-mouse (SouthernBiotech; 1031-30) and 10 μM Alexa Fluor® 647 anti-human (SouthernBiotech ...
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bioRxiv - Immunology 2021Quote: ... plates were washed with PBS-T and HRP-conjugated goat anti-mouse IgG or goat anti-human IgG (Southern Biotech, Birmingham, AL, USA) added for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... followed by two rounds of fluorescence-activated cell sorting (FACS) of cells stained with FITC-Conjugated rat anti-mouse IgG1 (Southern Biotech 1144-02), and rounds of soft-agar cloning in SeaPlaque agarose ...
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bioRxiv - Microbiology 2020Quote: ... plates were washed with PBS-T and HRP-conjugated goat anti-mouse IgG or goat anti-human IgG (Southern Biotech, Birmingham, AL, USA) added for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... IgM or IgA were detected by adding HRP-conjugated goat anti-mouse IgG (4 μg/ml; Southern Biotech, Cat#1030-05; RRID: AB_2619742), goat anti-mouse IgM (4 μg/ml ...
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bioRxiv - Microbiology 2021Quote: ... Goat anti-rabbit IgG-HRP (Cat No. 4030-05) and Goat anti-mouse IgG-HRP (Cat No. 1030-05) was from Southern Biotech (Alabama, USA). Goat anti-rabbit IgG Alexa fluor 488 (Cat no ...
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bioRxiv - Biochemistry 2020Quote: ... the plate was washed extensively (5×) with PBS-T and incubated with a HRP-conjugated mouse anti-human IgG (9040-05, clone JDC-10, Southern Biotech, Birmingham, AL) for 1 h at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Goat anti-rabbit IgG-HRP (Cat No. 4030-05) and Goat anti-mouse IgG- HRP (Cat No. 1030-05) was from Southern Biotech (Alabama, USA). U0126 (Cat no ...
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bioRxiv - Neuroscience 2023Quote: ... 50 µm mouse brain sections were rinsed in PBS for 5 minutes and blocked with PBS/5% normal goat serum (Southern Biotech, 0060-01) for 2 hours at room temperature ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed and incubated with either AP-conjugated goat anti-mouse IgG or IgM antibody (SouthernBiotech; cat # 1030-04, 1020-04) (Supplementary Table 8) ...
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bioRxiv - Microbiology 2024Quote: ... Plates were washed five times with PBS before adding 100 uL of HRP-conjugated goat anti-mouse IgG1 (Southern Biotech, Birmingham, AL, USA) diluted 1:4000 in blocking buffer and were then incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... biotin-conjugated secondary antibodies (1:200, SouthernBiotech) and avidin-biotin-peroxidase complex (Vectastain Elite ...
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bioRxiv - Microbiology 2023Quote: ... and individual IgG subclasses 1-4 (SouthernBiotech) were diluted to manufacturer recommended concentrations and incubated for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... NP (Southern Biotech, 10770-01, 1/1000), M2 (GeneTex ...
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bioRxiv - Immunology 2023Quote: ... or IgG2c (1:2,000 dilution; Southern Biotech). Peroxidase reactions were developed using OptEIA TMB substrate reagent set (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed with water three times before applying 25 μl of secondary antibody (goat anti-human IgG Fc-Southern Biotech, 2048-04; goat anti-mouse IgG Fc-Southern Biotech, 1033-04) at a dilution of 1:4,000 in blocking buffer ...
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bioRxiv - Immunology 2020Quote: ... Horse spleen ferritin-specific IgA levels in the supernatant of the faecal homogenates was determined by enzyme-linked immunosorbent assay in plates coated with horse spleen ferritin followed by detection with horseradish peroxidase-conjugated goat anti-mouse IgA (Southern Biotech, Birmingham, AL, USA) using optEIA (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... 96-well Immulon 4 HBX plates were coated overnight at 4°C with 5 mg/ml polyclonal goat-anti mouse κ (Southern Biotech; 1050-01). Nonspecific binding was blocked with 1% bovine serum albumin in PBS and samples were incubated at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Pellet was then resuspended in 100 µL ice-cold PBS containing 4 µg of FITC-coupled goat anti-mouse IgA antibody (SouthernBiotech, Imtec Diagnostic, #1040-02). The ratio of 4 µg of the respective antibody to stain 109 bacteria was previously evaluated to be the maximum amount of antibody that can be used without resulting in unspecific staining of non-IgA coated bacteria by using fecal samples from Rag1−/− mice as non IgA-coated negative controls ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed three times and then subsequently incubated for 1 h with 0.1 µg ml− 1 of anti-hamster IgG HRP (SouthernBiotech) in casein block at room temperature in the dark ...
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bioRxiv - Microbiology 2022Quote: ... then incubated in 0.1% TBST containing 1:4000 goat anti-rabbit antibody conjugated to HRP for 1 h at RT (SouthernBiotech).
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bioRxiv - Immunology 2021Quote: ... FO B cells and MZ B cells were cultured in RPMI medium + Pen/Strep + L-glutamine + 10% FBS and stimulated with polyclonal goat anti-mouse IgM (Southern Biotech; 0.6-150 ug/mL), or equimolar concentrations of the Fab’2 fragments (Southern Biotech ...
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bioRxiv - Immunology 2020Quote: ... or F(ab)2 polyclonal goat anti-human IgM (Jackson Immuno Research) as capture antibody and detection with HRP-conjugated mouse μ-specific anti-human IgM (Southern Biotech, clone SA-DA4).
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bioRxiv - Immunology 2020Quote: ... 4 single cell clones of DT40 WT or Fam72a−/− were stained with anti-IgM-PE (1:40, clone M-1, SouthernBiotech) and 12 populations of 5×104 IgM− cells were sorted for each clone ...
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bioRxiv - Microbiology 2020Quote: ... Cell lysates and supernatants were subjected to immunoblot analysis for antibodies against cellular LAMP-1 (1:2000, SouthernBiotech, 9835-01), VSV-G (1:2000 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with FACs buffer (1% BSA, 1% Na+azide) and secondary antibody IgG-PE (Southern Biotech, Alabama, USA) was added in a 1:200 dilution ...
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bioRxiv - Microbiology 2023Quote: ... formaldehyde fixed parasites were adhered to glass slides and blocked with 50% FCS in PBS for 45 min before staining with primary anti-VSG (1:10,000) and secondary Alexa Fluor (1:1,000) antibodies and mounting with DAPI Fluoromount-G® (Southern Biotech). Imaging was performed with an Axioscope 2 fluorescence microscope (Zeiss ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were mounted on glass slides in a 1:1 ratio of YC complete:DAPI Fluoromount-G (SouthernBiotech, Cat #0100-20). Yeast were imaged with a Zeiss 880 LSM with fast Airyscan.