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Citations for SouthernBiotech :
551 - 600 of 877 citations for Mouse Lymphoid Enhancer Binding Factor 1 LEF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Bound IgM monoclonal antibody was detected with PE-labelled goat anti-mouse IgM secondary antibody (Southern Biotech, Birmingham, AL, US) (Halpin et al. ...
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bioRxiv - Immunology 2022Quote: ... The bound rabbit plasma IgG was detected with a horseradish peroxidase (HRP)-conjugated polyclonal mouse anti-rabbit IgG (Southern Biotech) and the CytoGam was detected with an HRP-conjugated polyclonal goat anti-human IgG (Southern Biotech ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed and the samples were incubated with 50 μl of streptavidin-HRP diluted in blocking solution and standards with 50 μl of 0.175 μg/ml rat anti-mouse IgE-HRP (1130-05, Southern Biotech) diluted in blocking solution for 1 hour at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... the arrays were incubated with a mixture of goat anti-mouse IgM HRP (10 µg/mL; #1021-05 Southern Biotech) and donkey anti-goat antibody labeled with AlexaFluor555 (5 µg/mL ...
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bioRxiv - Microbiology 2023Quote: ... Bound antibodies were detected by using goat anti-mouse IgG conjugated to horseradish peroxidase (HRP; Southern Biotech, Birmingham, AL, USA) diluted 1:2000 in PBST and developed using 3,3’,5,5’-tetramethylbenzidine substrate (Thermo Scientific Pierce ...
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bioRxiv - Microbiology 2023Quote: ... was used except that plates were coated with anti-human kappa and lambda light chain specific mouse antibodies (Southern Biotech) at 1:1 ratio diluted to 1 μg/ml in PBS overnight ...
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bioRxiv - Microbiology 2023Quote: ... bound S8V1-157 was detected by incubating the microspheres with 2 μg/ml PE-conjugated rat anti-mouse IgG1 (SB77e, SouthernBiotech) for 1 hr at room temperature ...
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bioRxiv - Genomics 2022Quote: ... and incubated overnight with 1) goat anti-collagen I (1:200; SouthernBiotech; #1310-01) and rabbit anti-MFAP4 (1:100 ...
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bioRxiv - Immunology 2024Quote: ... IgG1 (1:5000, Southern Biotech), IgG2c (1:5000 ...
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bioRxiv - Immunology 2024Quote: ... IgG2c (1:5000, Southern Biotech) Ab for 1 hour at 30C ...
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bioRxiv - Cell Biology 2020Quote: ... and probed using the indicated primary antibodies (Table SX) and anti-mouse or anti-rabbit IgG-linked HRP secondary antibodies (Southern Biotech) followed by ECL (Luminata Crescendo or Forte ...
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bioRxiv - Immunology 2021Quote: ... washing with wash buffer (1xPBS+0.05% Tween) and incubation with secondary antibody - Mouse-anti-monkey IgG-HRP (Southern Biotech, 4700-05) for 1h ...
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bioRxiv - Immunology 2019Quote: ... Sera were incubated 2 h at 37°C on wells pre-coated with 5 μg/mL of NP15-BSA or goat-anti-mouse IgM (Southern Biotech) and saturated with PBS 2% BSA ...
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bioRxiv - Immunology 2019Quote: ... at 450 nm (595 nm reference wavelength) and then compared to a standard curve created with a mouse IgM standard (Southern Biotech) of known concentration.
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bioRxiv - Immunology 2019Quote: ... culture supernatants were incubated 2 h at 37°C on wells pre-coated with 5 μg/mL of goat-anti-mouse IgM (Southern Biotech) and saturated with PBS 2% BSA ...
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bioRxiv - Immunology 2020Quote: ... plates were coated with 2 µg/mL of goat anti-human kappa or goat anti-human lambda antibodies cross-absorbed against mouse IgG (Southern Biotech) at 4°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... Secondary antibodies used include goat anti-mouse IgG and goat anti rabbit-IgG both conjugated to horseradish peroxidase (HRP) (Southern Biotech). Forte Western HRP substrate (Millipore ...
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bioRxiv - Immunology 2022Quote: ... the hACE2 receptor bound to the S protein was then detected using a mouse anti-His-Tag horseradish peroxidase conjugate (Southern Biotech) and a colorimetric signal generated by the addition of 3,3′,5,5′-TMB substrate ...
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bioRxiv - Cancer Biology 2022Quote: ... Bound serum antibodies were then detected with a goat anti-mouse IgG-HRP secondary antibody (Southern Biotech 1030-05, Birmingham, AL) at 1:10,000 for 1 hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... 500 ng of F(ab’)2 fragments of α-μ (clone JDC-15; Dako [α-human]; OB1022; Southern Biotech [α-mouse]) and α-CD19 (clone HD37 ...
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bioRxiv - Immunology 2019Quote: ... Cells were washed with PBS–0.1% Tween 20 and incubated with mouse anti-human IgG Fc-HRP (Southern Biotech; 9040-05) for 1 hour ...
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bioRxiv - Immunology 2019Quote: ... Plates were washed with PBS–0.1% Tween 20 and incubated with mouse anti-human IgG Fc-HRP (Southern Biotech; 9040-05) for 1 hour ...
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bioRxiv - Bioengineering 2021Quote: ... arrays were incubated for an additional 2 h at RT and 60 rpm with goat anti-mouse IgG conjugated to HRP (Southern Biotech) diluted 1:5000 in blocking solution ...
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bioRxiv - Immunology 2021Quote: ... Bound IgA or IgM antibody was detected by incubation with anti-IgA-alkaline phosphatase (goat anti-mouse IgA (α chain-specific) (SouthernBiotech) or anti-IgM-alkaline phosphatase goat anti-mouse IgM (μ chain-specific ...
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bioRxiv - Immunology 2020Quote: ... The following day the plates were washed with PBS-T and incubated with goat anti-mouse IgG1-HRP (Southern Biotech, USA) at 1:6000 dilution in blocking buffer and left for 1 hour at 37°C ...
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bioRxiv - Immunology 2020Quote: An ELISA to detect anti-CCHFV Ig was performed as previously described (Hawman et al., 2019) with an anti-mouse Ig detection antibody (Southern Biotech) to detect all immunoglobulin isotypes or anti-mouse IgG or IgM (Southern Biotech ...
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bioRxiv - Immunology 2020Quote: ... followed by addition of HRP-conjugated goat anti-mouse IgG second antibody (catalog number 1030-05, Southern Biotech, Birmingham, AL, USA). TMB substrate was added and the plates read at OD 450 nm with a plate reader ...
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bioRxiv - Immunology 2022Quote: ... Hp-specific IgG1 was detected as previously described47 using plates coated with Hp extract and detected using rat anti-mouse IgG1-HRP antibody (Southern Biotech). NP-OVA specific IgG1 was detected as previously described48 using plates were coated with TNP(5)-BSA and detected using goat anti-mouse IgG1-HRP (Southern Biotech).
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bioRxiv - Immunology 2022Quote: ... NP-OVA specific IgG1 was detected as previously described48 using plates were coated with TNP(5)-BSA and detected using goat anti-mouse IgG1-HRP (Southern Biotech).
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bioRxiv - Immunology 2023Quote: ... and incubated with pleural lavage fluid overnight at 4°C prior to detection of bound IgM with a goat anti-mouse IgM-HRP (1020-05 Southern Biotech) for 1h at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... the wells were washed with the washing buffer twice and incubated with horseradish peroxidase (HRP)-conjugated mouse anti-monkey IgG (Southern Biotech) at room temperature for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... was added to the wells for 1h followed by washing and incubation with horseradish peroxidase-linked goat anti-mouse IgG secondary antibody (Southern Biotech). The avidity index was calculated as ...
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bioRxiv - Immunology 2022Quote: ... NP-specific IgM and IgG3 antibodies were detected using biotinylated anti-mouse IgM- or IgG3-specific immunoglobulins followed by streptavidin-HRP (Southern Biotech) and developed with SIGMAFAST OPD tablets (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... 1×109 bacteria were washed with 1 ml ice-cold PBS and stained for 30 min at 4°C with 4 µg of FITC-conjugated anti-mouse IgA antibody (Southern Biotech) in 100 µl of staining buffer ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed and incubated with a peroxidase-conjugated goat anti-mouse anti-IgM or anti-IgG antibody (Southern Biotech). After washing ...
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bioRxiv - Immunology 2023Quote: ... Bound IL-21 was subsequently detected with mouse anti-human IL-21-biotin (clone 2B2-G20, eBioscience) followed by alkaline phosphatase-conjugated streptavidin (Southern Biotech). The signals were compared to a standard curve generated with recombinant human IL-21 (R&D Systems).
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies including anti-mouse immunoglobulin G (IgG; 1010-05) and anti-rabbit IgG (catalog no. 4010-05) were obtained from Southern biotech. Primary antibodies were used at a 1:1000 dilution (unless otherwise stated) ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were washed with TBS-T 0.1% three times and incubated for one hr at room temperature with goat anti-mouse Ig horseradish peroxidase (HRP)-conjugated antibodies (Southern Biotech, anti-IgM secondary 1:1000 (cat# 1020-05) ...
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bioRxiv - Immunology 2024Quote: ... membranes were incubated with primary antibodies overnight at 4 °C and during 2 hours at room temperature with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit IgG (SouthernBiotech, USA). Membranes were then revealed using G:BOX Chemi XL1.4 Fluorescent & Chemiluminescent Imaging System (Syngene ...
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bioRxiv - Immunology 2022Quote: ... Antibody isotyping kit was purchased from Southern Biotech (Cat#5300-05). FITC-conjugated streptavidin was purchased from BD Biosciences (Cat#554060) ...
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bioRxiv - Neuroscience 2023Quote: ... anti-sheep (Novus NBP1-73267) or a 1:1 mix of anti-rabbit (Southern Biotech; OB405005) and anti-rabbit (Cell Signaling ...
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bioRxiv - Immunology 2019Quote: ... Murine antibodies were detected using alkaline phosphatase conjugated goat anti-mouse IgG or IgA (Southern Biotech, Cat #1030-04 and 1040-04). Rabbit antibodies were detected using alkaline phosphatase conjugated goat anti-rabbit IgG (Southern Biotech ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed with 0.05% Tween-20 in PBS and further incubated with HRP-conjugated goat antibodies directed against mouse IgG (Southern Biotech, Birmingham, AL) for 1 hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... plates were subsequently incubated with 0.1 µg/mL HRP-labeled goat anti-mouse κ light chain Ab (Southern Biotech, Birmingham, AL 35260), diluted in assay buffer for 1 hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... except constructs were bound directly to the plate and detected with goat anti-mouse IgG HRP conjugate (Southern Biotech, Birmingham, AL, USA).
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bioRxiv - Immunology 2021Quote: ... The secondary antibody used was horseradish-peroxidase (HRP)-conjugated goat polyclonal antibody against mouse IgG1 (1070-05; Southern Biotech, Cambridge, United Kingdom), HRP-conjugated goat polyclonal antibody against rabbit IgG (4050-05 ...
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bioRxiv - Immunology 2020Quote: ... plates were washed with PBS-T and HRP-conjugated goat anti-mouse IgG or anti-rat IgG (Southern Biotech, Birmingham, AL, USA) added for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... plates were washed 3 times and incubated with 50 μl of either 0.25 μg/ml biotinylated anti-mouse IgG1 (Southern Biotech, 1070-08), or biotinylated anti-mouse IgG (H+L ...
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bioRxiv - Immunology 2023Quote: ... Plates were then incubated for 90min at RT with goat anti-mouse IgG conjugated to horseradish peroxidase (HRP) (Southern Biotech, #1030-05) at 1:5000 dilution in blocking solution ...
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bioRxiv - Neuroscience 2021Quote: ... Collagen IV (SouthernBiotech, Goat 1:100), Thbs1 (Invitrogen ...