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Citations for SouthernBiotech :
551 - 600 of 643 citations for 8 BROMO 4 METHYLTHIO 7 PHENYLPYRAZOLO 1 5 A 1 3 5 TRIAZIN 2 AMINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Plates were washed with PBS + 0.05% Tween20 and binding was detected with anti-kappa HRP antibody (1:5000, Southern Biotech #2060-05) and developed with the TMB substrate (KPL (Mandel ...
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bioRxiv - Immunology 2022Quote: ... the cells were stained with 50μl/well of 1:200 diluted R-phycoerythrin (PE)-conjugated mouse anti-human IgG Fc antibody (SouthernBiotech cat.# 9040-09) and 1:1000 dilution of Zombie-NIR viability dye (BioLegend cat.# 423105 ...
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bioRxiv - Immunology 2023Quote: ... MAB273 was detected by adding a 1:5,000 dilution of monkey cross-adsorbed polyclonal goat anti-human IgG HRP antibody (Southern Biotech, 2049-05) and the signal was developed as described above.
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bioRxiv - Bioengineering 2023Quote: ... followed by 1 hr incubation with horseradish peroxidase-labeled goat anti-mouse secondary antibody (GAM-HRP, SouthernBiotech Cat#1010-05, RRID: AB_2728714). After washing ...
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bioRxiv - Immunology 2024Quote: ... plates were washed four times and 25 µl of a 1:500 dilution of the respective detection antibody (goat anti-mouse IgG(H+L)-alkaline phosphatase (AP) conjugate (Southern Biotech/Biozol) or goat anti-mouse IgG1-AP (Southern Biotech/Biozol ...
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bioRxiv - Neuroscience 2021Quote: ... we incubated the slice with DAPI Fluoromount G overnight at 4°C (Cat# 0100-20; SouthernBiotech, Birmingham, AL) to ensure the whole slice could be visualized under fluorescence microscopy to localize the biocytin-filled ...
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bioRxiv - Immunology 2020Quote: ... additions of either goat F(ab’)2 anti-human IgM LE/AF (Southern Biotech) at a final concentration of 10 µg mL-1 or media were made and the cells incubated for a further 18h at 37°C with 5% CO2.
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bioRxiv - Immunology 2021Quote: ... or equimolar concentrations of the Fab’2 fragments (Southern Biotech; 0.4-100 ug/mL) for 20 hours after which activation was measured using flow cytometry as described above.
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bioRxiv - Cancer Biology 2022Quote: ... Goat F(ab’)2 anti-human IgM was from Southern Biotech (Birmingham, AL, USA). BD Phosflow Fix Buffer I and Perm Buffer III were from BD Biosciences ...
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bioRxiv - Developmental Biology 2022Quote: ... Stained retinas were washed 3 times with PBS for 10 mins at room temperature and mounted using Fluoromount-G slide mounting medium (Southern Biotech). Flat Mount Retina Slides were proceeded to image with confocal microscopy (Zeiss).
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bioRxiv - Immunology 2020Quote: ... were incubated with serial 3-fold dilutions of either fecal supernatant (100 µL) or standard IgA (100 µL - SouthernBiotech, Birmingham, USA) for 1h30 at 37°C ...
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bioRxiv - Immunology 2021Quote: ... The reaction was visualized by subsequent addition of 2,2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) substrate (Southern Biotech, #0202-01).
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bioRxiv - Neuroscience 2020Quote: ... After thorough washing fluorescent secondary antibodies were applied at room temperature for 3 hours before counterstaining with DAPI and mounting with FluoroMount G (Southern Biotech). Slides were imaged using a Leica SP8 using 20x (NA 0.75 ...
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bioRxiv - Cell Biology 2020Quote: ... Then samples were washed three times with 0.1% Triton X-100 in PBS for 5 minutes and were incubated 30 minutes at RT with the following secondary antibody diluted 1:200: goat anti-rabbit Alexa Fluor 488 (Southern Biotech 4050-30), goat anti-mouse Alexa Fluor 488 (Southern Biotech 1030-30 ...
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bioRxiv - Immunology 2021Quote: ... Plates were again washed and then incubated with 1:4000 dilution of horse radish peroxidase (HRP) conjugated antimouse IgG secondary antibody (SouthernBiotech, cat. 1030-05) and incubated for 1h at RT ...
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bioRxiv - Bioengineering 2021Quote: ... The slides were subsequently incubated at room temperature with secondary antibodies: goat anti-rabbit Ig (1:100 dilution for 15 minutes, 4010-05, Southern Biotech, Birmingham, AL) for CD4 and goat anti-rabbit (1:50 dilution for 30 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... Antibodies bound to the thymocytes were detected by Cy5-conjugated goat anti-mouse IgG (1:250 dilution, 1030-15, Southern Biotech, Birmingham, AL) and Alexa Fluor 488-conjugated goat anti-mouse IgM (1:250 dilution ...
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bioRxiv - Immunology 2020Quote: ... the plates were first washed 5 times with PBS-T and then incubated for 1 hour at RT with HRP-conjugated anti-human IgG (Fc-specific, clone JDC-10, Southern Biotech, Birmingham, AL) diluted 3000 × in PBS-T ...
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bioRxiv - Immunology 2020Quote: ... the plates were washed at least 5 times with PBS-T and then incubated for 1 hour at RT with HRP-conjugated anti-human IgG (Fc-specific, clone JDC-10, Southern Biotech, Birmingham, AL) diluted 3000 × in PBS-T ...
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bioRxiv - Immunology 2021Quote: ... For detection of IgG subclasses all serum samples were diluted to 1 total IgG EU and incubated at 37 °C for 1 h prior to detection with Alkaline Phosphatase conjugated anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam) incubated for 1 h at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... all serum samples were diluted to 1 total IgG ELISA unit and then detected with anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam). The results of the IgG subclass ELISA are presented using OD values instead of the ELISA units used for the total IgG ELISA ...
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bioRxiv - Immunology 2022Quote: ... and the cells were stained with 50 μL/well of a 1:1,000 dilution of goat anti-human IgG-PE (Southern Biotech, cat. # 2040-09) secondary antibodies for 30 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... and stained with a 1:1,000 solution of goat anti-human IgG antibodies conjugated to phycoerythrin (PE) (Southern Biotech, Cat# 2040-09; RRID:AB_2795648) at 4°C for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... PrPSc on membranes was detected using mAb PRC5 at a dilution of 1:5000 followed by alkaline phosphatase conjugated goat anti mouse IgG (Southern Biotech, 1030-04) at a dilution of 1:5000 ...
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bioRxiv - Immunology 2023Quote: ... serum samples were applied in a 1:100 dilution and concentrations were determined from an IgG1 or IgG2a standard curve (Southern Biotech, Birmingham, USA). Horseradish peroxidase-linked goat anti-mouse IgG ...
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bioRxiv - Immunology 2023Quote: ... followed by probing the bound antibodies with an anti-monkey IgG horseradish peroxidase (HRP)-conjugated secondary antibody at 1:4000 dilution (Southern Biotech, Birmingham, AL) for 1hr at RT ...
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bioRxiv - Immunology 2023Quote: ... The SOSIP-specific IgG response in the serially diluted RM plasma was measured using an anti-monkey IgG horseradish peroxidase (HRP)-conjugated secondary antibody at 1:4000 dilution (Southern Biotech, Birmingham, AL) and concentration of the CH505-SOSIP-specific IgG response was estimated using a standard curve of CH505 T/F SOSIP IgG detection by Rh HIV-specific monoclonal antibody ...
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bioRxiv - Immunology 2023Quote: ... and the development of ADA in plasma samples were detected using a goat anti-human lambda IgG HRP-conjugated secondary antibody at 1:4000 dilution (Southern Biotech, Birmingham, AL). The plasma ADA levels were reported as endpoint titer ...
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bioRxiv - Neuroscience 2024Quote: ... They were then washed with PBS and incubated for 1 h at room temperature with the appropriate secondary antibody: goat anti-Rabbit IgG-HRP (Southern Biotech, #4030-05) or goat anti-Mouse IgG-HRP (Southern Biotech ...
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bioRxiv - Cell Biology 2021Quote: ... Afterward cells were fixed with 4% FA and mounted on glass slides using Fluoromount-G mounting medium (Southern Biotech). Images of the cells were acquired using confocal microscopy and analyzed with ZEN software (Carl Zeiss).
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bioRxiv - Cell Biology 2021Quote: ... Afterward cells were fixed with 4% FA and mounted on glass slides using Fluoromount-G mounting medium (Southern Biotech). Images of the cells were acquired using confocal microscopy and analyzed with ZEN software (Carl Zeiss).
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bioRxiv - Immunology 2019Quote: ... The sections were incubated with monoclonal antibodies (purchased from Southern Biotech, Cambridge, UK, Table 2) specific for various leukocyte subpopulations ...
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bioRxiv - Immunology 2021Quote: ... then incubated for 2 h at room temperature with Streptavidin-alkaline phosphatase from Southern Biotech at 2 μg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... 6-diamidino-2-phenylindole (DAPI)-containing mounting media (DAPI Fluoromount-G, 0100-20, Southern Biotech).
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bioRxiv - Developmental Biology 2023Quote: FISH-stained embryos and adults were mounted in 2 μL DAPI fluoromount-G (Southern Biotech) with 37.36 μm glass beads (Whitehouse scientific ...
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bioRxiv - Microbiology 2023Quote: ... cells were labeled with 2 μg/ml PE-conjugated goat anti-human IgG (Southern Biotech) for 30 min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... ELISA plates were coated with 2 µg/ml of goat anti-human IgG (Southern Biotech) at 4°C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... Retinas were again washed 3 times for 30 minutes each with PBS before a cover slip was attached with Fluoromount-G (SouthernBiotech, Birmingham, Alabama). For RGC counting ...
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bioRxiv - Microbiology 2020Quote: ... washed 3 times in ice-cold PBSM and then bound antibody detected with goat anti-human kappa-A647 (SouthernBiotech, cat# 2060-31) After washing ...
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bioRxiv - Immunology 2022Quote: ... was added to each well and incubated at RT for 3 minutes and the reaction was terminated with 50 μL of TMB Stop solution (SouthernBiotech, 0412-01). Absorbance at 450 nm (OD450 ...
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bioRxiv - Immunology 2023Quote: ... plates were washed 3 times and incubated with 50 μl of either 0.25 μg/ml biotinylated anti-mouse IgG1 (Southern Biotech, 1070-08), or biotinylated anti-mouse IgG (H+L ...
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bioRxiv - Cancer Biology 2022Quote: ... the coverslips were washed 3 times with PBS and mounted on microscopy slides using FluoromountG solution containing DAPI (Southern Biotech; 0100-01). Slides were imaged using a Zeiss 880 LSM with Airyscan microscope.
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bioRxiv - Neuroscience 2024Quote: ... Retinas were again washed 3 times for 30 minutes each with PBS before a cover slip was attached with Fluoromount-G (SouthernBiotech, Birmingham, Alabama). Images of immunostained wholemounts were acquired with a Keyence epifluorescence microscope (BZ-X800 ...
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bioRxiv - Immunology 2020Quote: ... washed 6 times and then incubated with 1: 6000 dilution of horseradish peroxidase (HRP) conjugated anti-mouse IgG secondary antibody (Southern Biotech; Birmingham, AL, USA) for 1 h at RT ...
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bioRxiv - Immunology 2020Quote: ... Plates were then incubated with a 1:1000 dilution of mouse serum raised against ZE3-6H followed by goat anti-mouse IgG HRP conjugate (Southern Biotech, Birmingham, AL, USA) for 1 h at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were fixed with 4% paraformaldehyde for 30 min at room temperature and stained with DAPI Fluoromount-G (Southern Biotech) to observe intracellular localization using an Olympus BX63 fluorescence microscope ...
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bioRxiv - Cancer Biology 2023Quote: ... the coverslips were fixed in 4% paraformaldehyde for 20 min at RT and mounted in DAPI Fluoromount-G (SouthernBiotech, USA). Slides were subjected to confocal imaging in a Zeiss LSM 700 microscope (Zeiss ...
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bioRxiv - Neuroscience 2022Quote: ... were applied for 2 hours then counterstained with Hoechst and mounted with Fluoromount-G (Southern Biotech). Z-stacks of explants were acquired on an Olympus Spinning Disk confocal microscope with Slidebook software 6.
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bioRxiv - Neuroscience 2024Quote: ... coverslips were mounted onto slides (Fisher 12-544-2) using Fluoromount gold (Southern Biotech 011-01) and dried overnight before storage at -20 °C.
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bioRxiv - Neuroscience 2022Quote: ... Slices were stored at 4°C until mounting on non-gelatin-coated slides followed by cover of DAPI fluoromount (Southern Biotech) and cover-slipped ...