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Citations for SouthernBiotech :
501 - 550 of 647 citations for IL 4 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... were coated overnight at 4°C with 2 μg/mL of monkey cross-adsorbed goat anti-human IgG (Southern Biotech #2049-01) diluted in phosphate-buffered saline (PBS) ...
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bioRxiv - Physiology 2020Quote: ... The sections were processed for immunostaining of Pan-CK (--) as described above with 4’,6-diamidino-2-phenylindole (DAPI) containing Fluormount-G (SouthernBiotech, Birmingham, AL) to visualize nuclei.
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bioRxiv - Immunology 2021Quote: ... Plates were then washed three times with PBS-T and secondary goat anti-mouse IgG-HRP antibody (Southern Biotech), diluted 1:4000 in ELISA buffer ...
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bioRxiv - Neuroscience 2021Quote: ... samples were labelled with biotin-coupled antibodies (all antibodies were purchased from Southern Biotech: goat anti mouse (1012-08), goat anti rat (3052-08) ...
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bioRxiv - Microbiology 2021Quote: ... samples were diluted at 1:50 and antigen-coupled microspheres were incubated with secondary antibody diluted at 1:100 each: mouse anti-human IgG1 hinge (0.1 mg/ mL, clone 4E3, SouthernBiotech); IgG2 Fc (0.1 mg/ mL ...
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bioRxiv - Microbiology 2020Quote: ... Plates were again washed with PBST and coated with 50 μL 1:10,000 diluted secondary antibody goat anti-mouse IgG HRP conjugated (1030-05, SouthernBiotech) and incubated 1 hr at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... dilution 1:10,000) and Goat Anti-Mouse IgG (H + L) Human ads-HRP (1031-05, dilution 1:10,000) (Southern Biotech); sheep anti-TGN38 (AHP499G ...
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bioRxiv - Immunology 2022Quote: ... Plates were then washed three times with PBS-T and secondary goat anti-mouse IgG-HRP antibody (Southern Biotech), diluted 1:4000 in ELISA buffer ...
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bioRxiv - Immunology 2021Quote: ... For the three-step protocol sections were stained using a goat anti-mouse IgG1-HRP-conjugated antibody (Southern Biotech) for CXCL12 and goat anti-mouse IgG2b-HRP-conjugated antibody (Southern Biotech ...
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bioRxiv - Immunology 2020Quote: ... HRP-conjugated secondary antibodies were used as follows: goat anti-mouse IgG-HRP (SouthernBiotech, Cat: 1030-05; 1:10,000) or goat anti-mouse IgA-HRP (SouthernBiotech ...
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bioRxiv - Immunology 2019Quote: ... HIV Env-specific IgG was then detected with phycoerythrin (PE)-conjugated mouse anti-human IgG (Southern Biotech, Birmingham, AL) at 2 μg/ml ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then surface stained with mouse anti-chicken Bu-1-FITC (Clone AV20; Southern Biotech, Birmingham, AL, USA) and mouse anti-chicken monocyte/macrophage-PE (Clone KUL01 ...
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bioRxiv - Immunology 2020Quote: ... coated with 100 µL/well of goat anti-mouse IgA (5 µg/mL Bic 0.1M, pH 9.6; Southern Biotech) were incubated with serial 3-fold dilutions of either fecal supernatant (100 µL ...
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bioRxiv - Immunology 2021Quote: ... RBC-depleted splenocytes were incubated with 1 μg/mL of goat F(ab’)2 anti-mouse Igκ-biotin (SouthernBiotech) for 30 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... the plates were incubated with a 1:2,000 dilution of a goat anti-mouse IgG2a-HRP antibody (Southern Biotech) in PBS supplemented with 1% FCS and incubated with gently shaking at RT for one hour ...
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bioRxiv - Immunology 2021Quote: ... Bound Abs were then reacted with HRP-conjugated goat anti-mouse IgG or IgM (Southern Biotech Cat.#1030-05) at 37lJ for 20 min ...
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bioRxiv - Immunology 2024Quote: ... with serum diluted 1:10 followed by an incubation with goat anti-mouse IgG-FITC (1:100; Southern Biotech). Images were acquired on an inverted fluorescence microscope (Olympus) ...
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bioRxiv - Immunology 2023Quote: ... The IgG assay contained a goat anti-mouse IgG Fc antibody (SouthernBiotech, 1033-31, labeled with Alexa Fluor 488) and the IgM assay a donkey anti-mouse IgM F(ab’)2 (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2022Quote: ... 2M7 mAb isolated from rabbit PBMCs was detected with an HRP-conjugated polyclonal mouse anti-rabbit IgG (Southern Biotech) and all the positive controls were detected with an HRP-conjugated polyclonal goat anti-human IgG (Southern Biotech ...
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bioRxiv - Microbiology 2023Quote: ... the plates were washed with washing buffer twice before adding the HRP-conjugated mouse anti-monkey IgG (Southern Biotech) and incubating at room temperature for 1 hour ...
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bioRxiv - Biophysics 2023Quote: ... at a 1:250 dilution and protein was detected with a HRP-conjugated goat anti-mouse antibody (Southern Biotech) using a 1:5000 dilution ...
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bioRxiv - Immunology 2022Quote: ... cells secreting anti-NP antibody (ASC) were visualized with HRP-conjugated anti-mouse IgM or IgG3 antibodies (Southern Biotech) followed by AEC staining Kit (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were stained again for 30 min with 0.5 µg of FITC-conjugated anti-mouse IgA antibody (Southern Biotech) in 100 µl of staining buffer to refresh and harmonize the staining between batches ...
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bioRxiv - Microbiology 2023Quote: ... 20 µl/well of a Phosphatase Alkaline-conjugated goat anti-mouse IgA (Southern Biotech, Imtec diagnostics, Ref: 1040-04), diluted 1/1000 in Dilution Buffer ...
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bioRxiv - Bioengineering 2024Quote: ... plates were washed with PBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG1 antibodies (Southern Biotech) or HRP-conjugated goat anti-mouse IgG2a-HRP antibodies (Southern Biotech ...
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bioRxiv - Bioengineering 2024Quote: ... Secondary antibody was horseradish peroxidase (HRP) conjugated anti-mouse IgG antibody and was procured from Southern Biotech (Birmingham AL). KPL TMB Stop Solution was purchased from Sera Care (Milford ...
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bioRxiv - Biochemistry 2024Quote: ... microspheres were incubated with 100 µL of goat anti-mouse IgG R-phycoerythrin (2 µg/mL, SouthernBiotech #1030-09) for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... samples were further statically incubated for 1 h at 4°C with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech, 1:300) and 6 μM Syto9 (Live/Dead BacLight Bacterial Viability Kit ...
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bioRxiv - Microbiology 2021Quote: ... secondary antibody at RT for 2h and stained with 4’,6-diamidino-2-phenylindole (DAPI) before mounting onto slides with Fluoromount-G (Southern Biotech, Birmingham, AL).
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bioRxiv - Molecular Biology 2020Quote: ... Worms were rinsed in PBS (3×10mins, 3 × 1hr) and equilibriated in mounting media with 4’,6-diamidino-2-phenylindole DAPI (Fluoromount G, Southern Biotech, Birmingham, AL) overnight before mounting and imaging.
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bioRxiv - Molecular Biology 2020Quote: ... and then 4 × 1 hour prior to counterstaining with the nuclear marker (DAPI) (1 ng/ml) and mounting in Fluoromount G (Southern Biotech, Birmingham, AL). The primary antibody used was anti-Schistosoma japonicum Ago2 (clone 650-1-1-KM864-3-11E8 ...
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bioRxiv - Immunology 2022Quote: ... cells were washed twice with FACS buffer and incubated for 30 min at 4°C with 1:1000 diluted PE-conjugated goat F(ab)’2 anti-human IgG (Southern Biotech 2042-09) in the dark ...
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bioRxiv - Immunology 2020Quote: ... The assay was performed as described above except for the secondary antibodies (1:2,000. goat Anti-Mouse IgG2a cross absorbed HRP antibody, Southern biotech, 1083-05 ...
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bioRxiv - Immunology 2021Quote: ... Wells were then washed 3 times with 200µL PBST before incubation for 1 hour with secondary antibodies (HRP-labeled Goat anti-mouse IgG1/IgG2b/IgG2c purchased from SouthernBiotech or HRP-labeled Goat anti-mouse IgG Fcγ purchased from Jackson ImmunoResearch ...
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bioRxiv - Immunology 2021Quote: ... then incubated with horseradish peroxidase (HRP)-conjugated mouse anti-monkey IgG (catalog number 4700-05, Southern Biotech, Birmingham, AL, USA) for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... and incubated with either rabbit anti-mouse IgG-HRP (Jackson Immuno) or goat anti-hamster IgG(H+L)-HRP (SouthernBiotech) diluted in casein for mouse or hamster samples ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed 3X with PBS and 3X with PBS-T and then anti-mouse IgG/IgA-biotin (Southern Biotech) diluted 1:1000 in PBS-T was added and incubated overnight at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed 3x and incubated with a 1:50,000 dilution of anti-mouse total IgG-HRP antibody (Southern Biotech) and incubated for 1h at 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... and then for 1 hour at room temperature with a 1:4000 diluted rabbit anti-mouse HRP secondary antibody (SouthernBiotech Cat# 6170-05 ...
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bioRxiv - Immunology 2021Quote: ... at 1×106 cells/well and stimulated for 48 hours with 5 μg/mL of RBD-His or hCD81-His or media alone (RPMI + 5% FCS) in the presence of anti-mouse CD28 antibody [0,5 μg/ml] (Southern Biotech). As positive control cells were stimulated with anti-mouse CD3 [0,05 μg/ml] (Southern Biotech) ...
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bioRxiv - Immunology 2020Quote: ... The membrane was washed in PBS containing Tween-20 (0.05%) and was incubated for 1 h with horseradish peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (Southern Biotech) diluted 1:20,000 accordingly ...
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bioRxiv - Immunology 2020Quote: ... Antibody was detected using an HRP-conjugated Goat anti-Mouse IgG kappa secondary antibody (Southern Biotech, PA1-86015, 1:1000) and developed with 1-Step Turbo TMB ELISA Substrate (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were stimulated with buffer (1% FBS/HBSS) or 10 μg/mL goat F(ab’)2 anti-mouse Igκ (SouthernBiotech) and Indo-1 fluorescence (violet vs blue ...
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bioRxiv - Immunology 2020Quote: ... washed thrice with PBST and detected with HRP-conjugated mouse anti-human IgG (Fc only) (Southern Biotech, Birmingham, AL, USA) antibody at a 1:2000 dilution ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 5 times with PBS-T and incubated with goat anti-mouse IgG1 antibody conjugated with horseradish peroxidase (SouthernBiotech) for 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Bound IgM monoclonal antibody was detected with PE-labelled goat anti-mouse IgM secondary antibody (Southern Biotech, Birmingham, AL, US) (Halpin et al. ...
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bioRxiv - Immunology 2022Quote: ... A volume of 25 μL of a 1:1,000 dilution of Mouse Anti-Biotin-AP (Southern Biotech, cat. #6404-04) in blocking buffer was added to each of the wells of the HN plates and incubated for 1 hr at RT ...
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bioRxiv - Microbiology 2022Quote: ... the plate was incubated in goat anti-mouse horseradish peroxidase (GAM-HRP) at a 1:5000 ratio (SouthernBiotech, Birmingham, AL). Lastly ...
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bioRxiv - Biochemistry 2024Quote: ... PVDF membranes containing blotted proteins were washed in excess PBS-Tween 20 for 1h at room temperature before application of a 1:2000 dilution of primary mouse Anti-Human IgA1 antibodies conjugated to biotin (SouthernBiotech). Blots were washed 10 min 3 x in PBS followed by treatment with a 1:2000 dilution of ExtrAvidin®−Peroxidase conjugate (Sigma ...
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bioRxiv - Immunology 2024Quote: ... Membranes were incubated in TBST containing 5% (w/v) skimmed milk with goat anti-mouse IgG conjugated to horseradish peroxidase (HRP) (1:2000, 1010-05, SouthernBiotech) for 3h at 4°C in a rotary platform and washed thrice with TBST for 10 min each time ...