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Citations for SouthernBiotech :
501 - 548 of 548 citations for Eukaryotic Peptide Chain Release Factor GTP Binding Subunit ERF3A GSPT1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Samples were then incubated with AlexaFluor™-conjugated secondary antibodies for two hours at room temperature and briefly rinsed with PBSTr and mounted with DAPI (Southern Biotech).
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bioRxiv - Immunology 2024Quote: ... goat F(ab′)2 anti-human kappa FITC and goat F(ab′)2 anti-human lambda FITC (SouthernBiotech; to detect antibody expression), and PI (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... plates were washed four times and 25 µl of a 1:500 dilution of the respective detection antibody (goat anti-mouse IgG(H+L)-alkaline phosphatase (AP) conjugate (Southern Biotech/Biozol) or goat anti-mouse IgG1-AP (Southern Biotech/Biozol ...
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bioRxiv - Cell Biology 2023Quote: ... The presence of the sLAP proteins was verified by western blotting according to standard protocols and by staining with anti-human HRP antibodies (Southern Biotech, USA). Concentration of sLAP proteins was quantified by dot blotting and using purified human Fc-IgG (SigmaAldrich ...
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bioRxiv - Immunology 2023Quote: ... MAB273 was detected by adding a 1:5,000 dilution of monkey cross-adsorbed polyclonal goat anti-human IgG HRP antibody (Southern Biotech, 2049-05) and the signal was developed as described above.
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bioRxiv - Bioengineering 2023Quote: ... followed by 1 hr incubation with horseradish peroxidase-labeled goat anti-mouse secondary antibody (GAM-HRP, SouthernBiotech Cat#1010-05, RRID: AB_2728714). After washing ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated with neoepitope-targeting mAbs and subsequently detected with a mouse anti-human IgG Fc-PE secondary antibody (SouthernBiotech 9040-09). Detection of the rabbit pAb cocktail was conducted with a goat anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: ... Then samples were washed three times with 0.1% Triton X-100 in PBS for 5 minutes and were incubated 30 minutes at RT with the following secondary antibody diluted 1:200: goat anti-rabbit Alexa Fluor 488 (Southern Biotech 4050-30), goat anti-mouse Alexa Fluor 488 (Southern Biotech 1030-30 ...
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bioRxiv - Immunology 2021Quote: ... Unbound antibodies were washed away with PBST and bound antibodies detected with 0.5 μg/mL HRP-conjugated goat anti-human constant kappa (2060-05, Southern Biotech, Birmingham, AL, USA) diluted in PBSTB ...
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bioRxiv - Immunology 2021Quote: ... Plates were again washed and then incubated with 1:4000 dilution of horse radish peroxidase (HRP) conjugated antimouse IgG secondary antibody (SouthernBiotech, cat. 1030-05) and incubated for 1h at RT ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were then washed with PBS-BSA and stained with secondary antibodies (5 μM Alexa Fluor® 488 anti-mouse (SouthernBiotech; 1031-30) and 10 μM Alexa Fluor® 647 anti-human (SouthernBiotech ...
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bioRxiv - Microbiology 2021Quote: ... samples were further statically incubated for 1 h at 4°C with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech, 1:300) and 6 μM Syto9 (Live/Dead BacLight Bacterial Viability Kit ...
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bioRxiv - Bioengineering 2021Quote: ... The slides were subsequently incubated at room temperature with secondary antibodies: goat anti-rabbit Ig (1:100 dilution for 15 minutes, 4010-05, Southern Biotech, Birmingham, AL) for CD4 and goat anti-rabbit (1:50 dilution for 30 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... Antibodies bound to the thymocytes were detected by Cy5-conjugated goat anti-mouse IgG (1:250 dilution, 1030-15, Southern Biotech, Birmingham, AL) and Alexa Fluor 488-conjugated goat anti-mouse IgM (1:250 dilution ...
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bioRxiv - Immunology 2021Quote: ... For detection of IgG subclasses all serum samples were diluted to 1 total IgG EU and incubated at 37 °C for 1 h prior to detection with Alkaline Phosphatase conjugated anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam) incubated for 1 h at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... all serum samples were diluted to 1 total IgG ELISA unit and then detected with anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam). The results of the IgG subclass ELISA are presented using OD values instead of the ELISA units used for the total IgG ELISA ...
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bioRxiv - Immunology 2022Quote: ... and stained with a 1:1,000 solution of goat anti-human IgG antibodies conjugated to phycoerythrin (PE) (Southern Biotech, Cat# 2040-09; RRID:AB_2795648) at 4°C for 30 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Staining was performed using anti-PrP antibody (clone ICSM18; D-Gen Ltd) followed by detection with alkaline-phosphatase-linked anti-IgG1 antiserum (Southern Biotech; 1070-04). Spots were visualised with alkaline phosphatase conjugate substrate (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... They were then washed with PBS and incubated for 1 h at room temperature with the appropriate secondary antibody: goat anti-Rabbit IgG-HRP (Southern Biotech, #4030-05) or goat anti-Mouse IgG-HRP (Southern Biotech ...
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bioRxiv - Immunology 2023Quote: Biotinylated Goat F(ab)2 anti-human isotype antibodies (anti-human IgM, SouthernBiotech, Cat # 2022-01; anti-human IgA, SouthernBiotech, Cat # 2052-01, and anti-human IgG, SouthernBiotech, Cat # 2042-01) were conjugated on to selected peaks of Streptavidin coated 4um polystyrene capture beads ...
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bioRxiv - Immunology 2023Quote: ... and incubated with 0.75x serial dilutions of purified polyclonal human antibodies (IgG, SouthernBiotech, Cat # 0150-01; IgM, SouthernBiotech, Cat # 0158L-01; IgA, SouthernBiotech, Cat # 0155K-01) at known concentrations ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed and incubated with either AP-conjugated goat anti-mouse IgG or IgM antibody (SouthernBiotech; cat # 1030-04, 1020-04) (Supplementary Table 8) ...
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bioRxiv - Immunology 2023Quote: ... and the development of ADA in plasma samples were detected using a goat anti-human lambda IgG HRP-conjugated secondary antibody at 1:4000 dilution (Southern Biotech, Birmingham, AL). The plasma ADA levels were reported as endpoint titer ...
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bioRxiv - Immunology 2023Quote: ... The SOSIP-specific IgG response in the serially diluted RM plasma was measured using an anti-monkey IgG horseradish peroxidase (HRP)-conjugated secondary antibody at 1:4000 dilution (Southern Biotech, Birmingham, AL) and concentration of the CH505-SOSIP-specific IgG response was estimated using a standard curve of CH505 T/F SOSIP IgG detection by Rh HIV-specific monoclonal antibody ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated for 1 hour at room temperature in TBSX with 1:1000 donkey fluorescent secondary antibody and mounted on slides using Fluoromount-G (Southern Biotech 0100-01) before coverslipping ...
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bioRxiv - Immunology 2024Quote: ... Blocking and primary sample incubations were one hour at 37°C and secondary antibody incubations with a 1:6,000 dilution of mouse anti-monkey HRP conjugate (clone SB108a, Southern Biotech, cat. 4700-05) were 45 min at 37°C ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed in TBS-T and incubated with horseradish peroxidase (HRP)-conjugated goat anti-human Ig Fc antibody (Southern Biotech, 2047-050) at a dilution of 1:4000 in TBS-TMS for 30 min at RT ...
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bioRxiv - Cell Biology 2024Quote: ... the membrane was washed five times in PBS-T before it was incubated with the secondary antibody goat α-rat IgG (H&L)-HRP (1:10,000, Southern Biotech #3050-05) or goat α-mouse IgG (H&L)-HRP (1:10,000 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The secondary antibodies were goat anti-rabbit (#4010-05, 1:5000) and anti-mouse (#1030-05, 1:5000, both from Southern Biotech, Birmingham AL) coupled to horseradish peroxidase ...
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bioRxiv - Immunology 2024Quote: ... 100 μl per well of 1 μg/l alkaline phosphatase (AP)-conjugated goat anti-human antibodies (Goat Anti-Human kappa-AP, SouthernBiotech, ref 2060-04) was incubated in PBS with 0.05% Tween® 20 for 2 h at 37°C ...
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bioRxiv - Immunology 2024Quote: ... coated overnight at 4°C (100 μl per well) with 2 μg/ml Igκ antibodies (Goat Anti-Human kappa-UNLB, SouthernBiotech, ref 2060-01) in PBS ...
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bioRxiv - Immunology 2024Quote: ... membranes were incubated with indicated primary antibodies overnight at 4 °C and with horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (SouthernBiotech, Birmingham, AL, USA) for 2 hours at room temperature ...
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bioRxiv - Immunology 2024Quote: Fifty microliters of appropriately diluted serum samples were added to 96-well plates precoated with anti-mouse Igκ and Igλ antibodies (2 mg/ml; Southern Biotech, Birmingham, AL) in 0.1 M carbonate buffer (pH 9.0) ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were transferred to nitrocellulose or PVDF membrane and probed with rat anti-FLAG (clone 9H1, produced in-house; 1:1000) primary antibody and anti-rat HRP (Southern Biotech; 1:5000) secondary.
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bioRxiv - Cell Biology 2024Quote: ... The secondary antibodies (1:10,000 working concentration) for immunoblot were horseradish peroxidase (HRP)-conjugated goat anti-rat immunoglobulin (Ig) (Southern BioTech Cat#3010-05), HRP-conjugated goat anti-rabbit Ig (Southern BioTech Cat#4010-05) ...
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bioRxiv - Biochemistry 2020Quote: ... the culture media were tested for their ACE2-Fc contents using a custom ELISA (anti-ACE2 antibody [Abcam, Cambridge, MA] for capturing and anti-human IgG-Fc-HRP [SouthernBiotech, Birmingham, AL] for detection). Clones with the highest expression of recombinant ACE2-Fc variants were selected ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed with water three times before applying 25 μl of secondary antibody (goat anti-human IgG Fc-Southern Biotech, 2048-04; goat anti-mouse IgG Fc-Southern Biotech, 1033-04) at a dilution of 1:4,000 in blocking buffer ...
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bioRxiv - Immunology 2020Quote: ... washed 6 times and then incubated with 1: 6000 dilution of horseradish peroxidase (HRP) conjugated anti-mouse IgG secondary antibody (Southern Biotech; Birmingham, AL, USA) for 1 h at RT ...
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bioRxiv - Immunology 2021Quote: ... Sections were washed in PBS before incubation with secondary antibodies (Table 3) for 1 hr at room temperature followed by mounting with DAPI containing fluoromount (Southern Biotech, Birmingham, AL, USA). Images were captured with an EVOS FL imaging system (ThermoFisher Scientific) ...
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bioRxiv - Physiology 2023Quote: ... The membranes were then washed 3x in TBST before incubation for 1 hour at room temperature with peroxidase-conjugated secondary antibodies in TBST at 1:10,000 (Southern Biotech, #1031-05, #4050-05). Antibody binding was detected using an enhanced chemiluminescence HRP substrate detection kit (Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... Paraffin sections of kidneys were used to stain for collagen type IV using a goat polyclonal collagen IV antibody (Southern Biotech, Birmingham, AL, USA) (Thallas-Bonke et al. ...
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bioRxiv - Microbiology 2023Quote: ... Pellet was then resuspended in 100 µL ice-cold PBS containing 4 µg of FITC-coupled goat anti-mouse IgA antibody (SouthernBiotech, Imtec Diagnostic, #1040-02). The ratio of 4 µg of the respective antibody to stain 109 bacteria was previously evaluated to be the maximum amount of antibody that can be used without resulting in unspecific staining of non-IgA coated bacteria by using fecal samples from Rag1−/− mice as non IgA-coated negative controls ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated in the secondary antibody at room temperature and washed with TBS prior to slide-mounting with DAPI Fluoromount-G® mounting medium (SouthernBiotech, catalog #0100-20). Primary antibodies included rabbit anti-DsRed (Takara Bio ...
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bioRxiv - Immunology 2024Quote: ... The plates were washed before the addition of goat anti-mouse IgG secondary antibody conjugated with alkaline phosphatase (AP) (Southern Biotech, Birmingham, AL, USA) for 2 hr ...
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bioRxiv - Immunology 2020Quote: ... or F(ab)2 polyclonal goat anti-human IgM (Jackson Immuno Research) as capture antibody and detection with HRP-conjugated mouse μ-specific anti-human IgM (Southern Biotech, clone SA-DA4).
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bioRxiv - Microbiology 2024Quote: ... Cells were incubated cell side down in 100 µL of Stellaris RNA FISH Hybridization Buffer (BioSearch) containing M1 vRNA probes (1/100) and the primary antibodies of interest (NP, Southern Biotech, 10770-01, 1/100) and incubated in a humid chamber at 37°C in the dark for 16 h ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated at RT for 1 h with the following HRP-conjugated secondary antibodies: goat anti-rabbit IgG conjugated to HRP (Southern Biotech, 4010-05; 1:5000), and goat anti-mouse IgG conjugated to HRP (Southern Biotech ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed with 1% BSA in PBS twice before incubating with secondary anti-rat IgG antibody conjugated with APC (1:500; Southern Biotech, AL, USA, Catalog no. 3010-11L). Cells were analyzed in BD FACSVerse™ flow cytometer (BD Biosciences ...