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Citations for SouthernBiotech :
451 - 500 of 524 citations for 1 3 Methoxyphenyl 6 7 dimethyl 6 azoniabicyclo 3.2.1 octane bromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... followed by the addition of horseradish peroxidase-conjugated goat anti-mouse-IgG Fc secondary antibody (1:2000) (Southern Biotech, 1033-05). After incubation for 2 hours at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... in PBST or PBSTF or 1:1000 anti-hamster-IgG2/IgG3-HRP in PBST or PBSTF (Southern Biotech Cat. #1935-05). Plates were incubated at room temperature for 1-2 h ...
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bioRxiv - Immunology 2022Quote: ... The plates were then washed with PBS containing 0.1% Tween-20 (PBS-T), and Alkaline Phosphatase-conjugated Goat Anti-Human IgG (dilution 1:500, catalog no. 2040-04) from Southern Biotech was added and incubated for 45 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Antibody binding to the spike proteins was determined using a 1:2000 diluted HRP conjugated goat anti-human IgG (ITK Southern Biotech) for 1 h at RT and tetramethylbenzidine substrate (BioFX) ...
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bioRxiv - Microbiology 2020Quote: ... the peptide arrays were incubated with a 1/1000 dilution of an appropriate antibody peroxidase conjugate (goat anti-human HRP conjugate, Southern Biotech, cat ...
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bioRxiv - Microbiology 2021Quote: ... Fab fragments were detected with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech, 1:500). After washing ...
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bioRxiv - Immunology 2021Quote: ... membranes were incubated with secondary antibodies conjugated to alkaline phosphatase anti-IgM or anti-IgG diluted 1:2000 (rabbit anti-mouse IgM, Jackson ImmunoResearch, goat anti-mouse IgG, SouthernBiotech). Substrate NBT/BCIP (Promega ...
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bioRxiv - Immunology 2021Quote: ... incubated for 1 hour at room temperature with mouse anti-human IgG conjugated to horse radish peroxidase (Southern Biotech, Birmingham, USA) diluted 1:5000 in blocking solution ...
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bioRxiv - Microbiology 2023Quote: ... Alexa Fluor® Plus 488 secondary antibodies were diluted 1:1,000 in the respective blocking solutions before mounting with 5 µL DAPI Fluoromount-G® (Southern Biotech) and imaging ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed in PBS and incubated in secondary antibody solution for 1 hour before being mounted on Superfrost Plus slides and coverslipped with DAPI-containing Fluoromount-G (0100-20, Southern Biotech).
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bioRxiv - Microbiology 2023Quote: ... anti-IgG2 Fc-Alexa Fluor 488 and mouse anti-IgG3 hinge-Alexa Fluor 647 (1 µg/ml, all from Southern biotech) for an additional 20 minutes at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Precomplexation of the anti-sialyl-LewisX antibody was performed with a 1:50 dilution of goat anti-mouse IgM-HRP (#1021-05, Southern Biotech) and 0.65 µg donkey anti-goat Alexa Fluor 555 (A21432 ...
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bioRxiv - Molecular Biology 2023Quote: ... and primary antibodies were added in 1 : 10000 dilution (anti-IgG Fc cat. 31789, Thermo Fisher; anti-His-Tag cat. 4603-08, SouthernBiotech) followed by 1 h incubation at RT ...
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bioRxiv - Neuroscience 2023Quote: ... diluted 1:1000 in PBST in order to detect the primary antibody for 1 h at room temperature before being mounted on glass slides and coverslipped with Fluoromount-G (0100-01, Southern Biotech). Images of cresyl violet stained sections were obtained using an Axio Observer Carl Zeiss light microscope whereas fluorescent images from immunolabelled sections were collected using an Axio Observer Carl Zeiss fluorescent microscope ...
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bioRxiv - Microbiology 2024Quote: ... Following additional washings and incubation at 37°C for 1 h with AP-conjugated anti-goat IgG secondary antibody (Southern Biotech), absorbance was read by addition of PNPP ...
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bioRxiv - Immunology 2022Quote: ... at 37 °C for 1 hr followed by the multi-species SP ads-HRP-conjugated goat anti-mouse IgG (H+L) antiserum (Southern Biotech) diluted at 1:20.000 and incubated at 37 °C for 1 hr ...
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bioRxiv - Physiology 2023Quote: ... Non-specific antibody binding was blocked for 1 hour at room temperature with a blocking solution containing: 5% normal goat serum (006001, SouthernBiotech™), 0.3% Triton™ X-100 (X100 ...
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bioRxiv - Biochemistry 2023Quote: ... then washed and secondary antibody added at 1:2000 or 1:4000 dilution in blocking buffer (100□μl/well) using either anti-mouse IgG-HRP (Catalog #1030-05, Southern Biotech), or anti-mouse IgA-biotin (Catalog #1040-80 ...
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bioRxiv - Cell Biology 2023Quote: ... Labeling of primary antibodies was visualized with anti-mouse PE and anti-rat PE (both Southern Biotech, USA, 1:1000 diluted). Actin in living cells was visualized with SiR actin (Spirochrome ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies conjugated with HRP used in the study are: Goat Anti-mouse IgG1-HRP (1:10000, Southern Biotech, 1071-05), Goat Anti-rabbit IgG-HRP (1:10000 ...
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bioRxiv - Immunology 2024Quote: ... diluted to 1 μg/ml in T-PBS followed by incubation for 1 hour with goat-anti mouse IgG conjugated with HRP (Southern Biotech) diluted 2000 × in T-PBS.
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bioRxiv - Immunology 2024Quote: ... diluted to 1 μg/ml in T-PBS followed by incubation for 1 hour with goat-anti mouse IgG conjugated with HRP (Southern Biotech) diluted 2000 × in T-PBS ...
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bioRxiv - Immunology 2024Quote: ... Specific IgG was detected for 1 hour at room temperature with biotin rat anti-mouse IgG (Southern Biotech, Birmingham, AL, USA) biotin rat anti-mouse IgG1 (Southern Biotech) ...
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bioRxiv - Immunology 2024Quote: ... samples were incubated with 100 μl of a 1:500 diluted phycoerythrin (PE)-tagged goat-anti mouse IgG-Fc (Southern Biotech) in the dark at RT with shaking at 600 rpm for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... Wells then were incubated for 1 hour with horseradish peroxidase (HRP)-conjugated goat anti-human IgG (monkey pre-adsorbed) (Southern Biotech) at a 1:4,000 dilution ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed with ELISA wash buffer then incubated for 1 h at RT with an HRP conjugated goat anti-mouse (Southern Biotech). Plates were washed with ELISA wash buffer and developed using 1-Step Ultra TMB-ELISA substrate solution (Thermo Fisher) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed 3x with DPBS and coverslips were mounted on 1 mm glass slides using Fluoromount-G (SouthernBiotech, 0100-01).
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bioRxiv - Biochemistry 2021Quote: ... 1×107 cells were starved for 30 min at 37°C in 1 ml FCS-free RPMI and then treated with 10 µg/ml anti-λ antibody (Southern Biotech) under shaking at 750 rpm for the indicated time points ...
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bioRxiv - Cell Biology 2020Quote: ... samples were incubated for 30 minutes with the following secondary antibodies diluted in PBS 2.5% BSA: donkey anti-goat Alexa Fluor 488 1:200 (Southern Biotech 3425-30) and donkey anti-rabbit Alexa Fluor 555 1:200 (Thermo fisher scientific A32794) ...
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bioRxiv - Microbiology 2021Quote: ... The primary antibodies were detected by secondary antibody anti-mouse or anti-rabbit conjugated to horseradish peroxidase (Southern Biotech, 1:10,000 dilution) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... the wells were incubated for 2 h at 37°C with HRP-mouse anti-human IgG antibody (dilution 1:2,000) (Southern Biotech, Birmingham, AL) followed by the peroxidase substrate ...
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bioRxiv - Molecular Biology 2022Quote: ... once with 0.01X Wash Buffer B for 1 min at room temperature and coverslips were mounted on the slide with DAPI Fluoromount-G mounting medium (SouthernBiotech, Birmingham, USA).
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bioRxiv - Immunology 2022Quote: ... plates were washed 4× with ELISA wash buffer and a 1:4000 dilution of goat anti-mouse IgG (SouthernBiotech, catalog #1030-05) in ELISA blocking buffer was added to each well and incubated at 37°C for 1 hour followed by 4 washes with ELISA wash buffer ...
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bioRxiv - Microbiology 2022Quote: ... the beads were incubated with 1 µg/mL Alexa Fluor647-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech, 2062-31) in PBS-TH for 30 min at 4 °C with shaking ...
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bioRxiv - Biochemistry 2022Quote: ... and then treated with alkaline phosphatase (AP)-conjugated goat anti-rabbit-IgG (Southern Biotech; 1:8000 in TBST with 2% (w/v) bovine serum albumin) ...
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bioRxiv - Developmental Biology 2022Quote: ... then incubated in fresh dilution solution with secondary antibody (goat anti-mouse IgG2a AlexaFluor 488, 1:250, Southern Biotech, cat# 1080-30) overnight at 4°C with gentle shaking ...
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bioRxiv - Immunology 2020Quote: ... plates were coated with 1:2000 (for capture of polyclonal macaque IgG) or 1:1000 (for capture of polyclonal human IgG) of goat anti-Human IgG λ and K (Southern Biotech). The next day ...
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bioRxiv - Biochemistry 2020Quote: ... for 1 min, washed with PBS again (3X, 5 min) and slides were mounted with Fluoromount-G mounting medium (SouthernBiotech, Birmingham, AL). Images were acquired on a Leica SP5 confocal microscope and analyzed by Fiji ImageJ.
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bioRxiv - Immunology 2020Quote: ... The plates were washed twice in FACS buffer and stained with 50 μl/well of 1:200 dilution of R-phycoerythrin (PE)-conjugated mouse anti-human IgG Fc antibody (SouthernBiotech #9040-09) and 1:1000 dilution of Zombie-NIR viability dye (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... plates were washed four times and 25 µl of a 1:500 dilution of the respective detection antibody (goat anti-mouse IgG(H+L)-alkaline phosphatase (AP) conjugate (Southern Biotech/Biozol) or goat anti-mouse IgG1-AP (Southern Biotech/Biozol ...
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bioRxiv - Bioengineering 2023Quote: ... followed by 1 hr incubation with horseradish peroxidase-labeled goat anti-mouse secondary antibody (GAM-HRP, SouthernBiotech Cat#1010-05, RRID: AB_2728714). After washing ...
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bioRxiv - Cancer Biology 2022Quote: ... Plates were washed with PBS + 0.05% Tween20 and binding was detected with anti-kappa HRP antibody (1:5000, Southern Biotech #2060-05) and developed with the TMB substrate (KPL (Mandel ...
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bioRxiv - Immunology 2022Quote: ... the cells were stained with 50μl/well of 1:200 diluted R-phycoerythrin (PE)-conjugated mouse anti-human IgG Fc antibody (SouthernBiotech cat.# 9040-09) and 1:1000 dilution of Zombie-NIR viability dye (BioLegend cat.# 423105 ...
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bioRxiv - Immunology 2023Quote: ... MAB273 was detected by adding a 1:5,000 dilution of monkey cross-adsorbed polyclonal goat anti-human IgG HRP antibody (Southern Biotech, 2049-05) and the signal was developed as described above.
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bioRxiv - Immunology 2024Quote: ... Plates were then washed 3x with wash buffer and incubated with a 1:4,000 dilution (in EZ block) of Goat Anti-Mouse IgG Fc-HRP secondary (Southern Biotech, 1033-05) for 1 hr at RT ...
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bioRxiv - Immunology 2024Quote: ... Plates were subsequently washed three times prior to the addition of streptavidin-alkaline phosphatase (1:2000, Southern Biotech, Birmingham, AL, United States) to all wells for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Then samples were washed three times with 0.1% Triton X-100 in PBS for 5 minutes and were incubated 30 minutes at RT with the following secondary antibody diluted 1:200: goat anti-rabbit Alexa Fluor 488 (Southern Biotech 4050-30), goat anti-mouse Alexa Fluor 488 (Southern Biotech 1030-30 ...
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bioRxiv - Immunology 2021Quote: ... Plates were again washed and then incubated with 1:4000 dilution of horse radish peroxidase (HRP) conjugated antimouse IgG secondary antibody (SouthernBiotech, cat. 1030-05) and incubated for 1h at RT ...
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bioRxiv - Bioengineering 2021Quote: ... The slides were subsequently incubated at room temperature with secondary antibodies: goat anti-rabbit Ig (1:100 dilution for 15 minutes, 4010-05, Southern Biotech, Birmingham, AL) for CD4 and goat anti-rabbit (1:50 dilution for 30 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... Antibodies bound to the thymocytes were detected by Cy5-conjugated goat anti-mouse IgG (1:250 dilution, 1030-15, Southern Biotech, Birmingham, AL) and Alexa Fluor 488-conjugated goat anti-mouse IgM (1:250 dilution ...