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Citations for Millipore Sigma :
651 - 700 of 2519 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Expression of genes of interest was measured using primers designed with the NCBI Primer designing tool and custom-synthesized by Sigma-Aldrich. We list primers used in the present study (Table S9) ...
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bioRxiv - Synthetic Biology 2022Quote: ... based plasmid encoding GR1-CFP was generated in a prior study.21 Three forward primers and one reverse primer were synthesized using the 22c-trick (Sigma-Aldrich) to generate each SSM library as previously described (Table S2).21,59 E ...
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bioRxiv - Genetics 2021Quote: All ORF-specific PCR primer sequences used in this validation are shown in Additional file 1: Table S3 and primers were obtained from Millipore Sigma unless mentioned otherwise from Integrated DNA Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... Sections were washed two times with PBS and incubated for two hours at RT with corresponding secondary antibodies and subsequently incubated for 30 min at RT with DAPI (Sigma). Stained sections were washed two times with PBS and mounted with either Fluorsave (Chemicon ...
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bioRxiv - Microbiology 2020Quote: ... RT SigmaFast O-phenylenediamine (OPD) substrate (P9187, Sigma) was added to the wells after six additional washes to develop the colorimetric reaction ...
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bioRxiv - Physiology 2021Quote: ... For the RT-qPCR the Kappa Mix (Sigma) was used and the primers concentration was 10 µM ...
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bioRxiv - Cell Biology 2023Quote: ... permeabilized at RT with 0.25% TritonX100 (Sigma #T9284) for 10 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... and 50 mg Fast Blue RT (Sigma-Aldrich) in 100 ml of 50 mM 2-amino-2-methyl-1,3-propanediol buffer (pH 9.8) ...
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bioRxiv - Cell Biology 2023Quote: ... After fixation cells were placed in hybridization buffer (5% dextran sulfate, 50% formamide, 5× SSC, 100 μg/ml heparin, 100 μg/ml sonicated salmon sperm DNA (Sigma-Aldrich, cat. No. D9156),0.1% Tween-20 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then spun down at 7,000g for 8 minutes and resuspended in 100 μL secondary hybridization buffer (10% Formamide, 2X SSC, 10% Ficoll PM-400 (Sigma-Aldrich F5415-25 mL)) ...
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bioRxiv - Cancer Biology 2023Quote: ... we mounted the slides in an anti-bleach imaging buffer containing 2X SSC/10 mM Tris-HCl/10 mM Trolox (Sigma Aldrich, cat. no. 238813)/37 ng/μL glucose oxidase (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... for 3 min at 95 °C, and then mixed with 50 μL hybridization buffer H (4x SSC, 4 mg/ml acetylated BSA (Sigma Fluka Aldrich, B8894-1.5mL), 20 mM vanadyl ribonuclease complex) ...
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bioRxiv - Cancer Biology 2021Quote: RIP assays were conducted to explore the relationship between lncRNA NEAT1 and miR-1299 via the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, MA, USA). Initially ...
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bioRxiv - Microbiology 2022Quote: ... and oligo(dT) primers (Eurofins Genomics) for detection of mRNA or a fluA uni12 forward primer [92] (Sigma-Aldrich, 5’-AGCAAAAGCAGG-3’) to detect vRNA according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and treated with DNAse (On-Column DNase I Digestion set, Sigma, Inc., USA). cDNA libraries were prepared with the TruSeq RNA sample Preparation v2 kit (Illumina Inc. ...
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bioRxiv - Bioengineering 2022Quote: ... Another set of cleaned coverslips was treated with poly-L-lysine (Sigma Aldrich) for 45 minutes at 37°C in a humid chamber ...
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bioRxiv - Genetics 2022Quote: ... The kidneys were set up for culture on Whatman membranes (Sigma, catalog# WHA110409) in an air-medium interface as described26 ...
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bioRxiv - Cell Biology 2022Quote: ... plus protease inhibitor cocktail Set I-Calbiochem 1:100 (Cat# 539131, Merck Millipore), 1 mM Na3VO4 and 1mM NaF) ...
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bioRxiv - Microbiology 2020Quote: The magnetic extraction set-up consisted of three 3 mL cuvettes (Sigma Aldrich) and a neodymium magnet (60×10×3 mm ...
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bioRxiv - Systems Biology 2020Quote: ... An initial set of cultures were inoculated into LB media (Lennox, Sigma-Aldrich) containing 25 μg/mL chloramphenicol (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... a specific short hairpin RNA (shRNA) construct set was obtained from Sigma-Aldrich, and lentivirus production plasmids psPAX2 and pMD2.G were obtained from Addgene ...
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bioRxiv - Genomics 2024Quote: The 1st and 2nd sets of Cell Barcodes are synthesized by Sigma-Aldrich. The 3rd set of Cell Barcodes are synthesized by IDT.
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bioRxiv - Cancer Biology 2020Quote: Reverse transcription (RT) reactions were carried out using 1 µg of RNA using Transcriptor RT enzyme (Catalog No. 03531287001) from Sigma Aldrich. IR minigene PCRs were performed as previously reported ...
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bioRxiv - Cell Biology 2020Quote: ... Primers used for cDNA amplification were purchased from Sigma and are listed in Supplemental Table 1.
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bioRxiv - Genetics 2021Quote: ... Primers (Supplementary Table 4) were purchased from Sigma Aldrich; the reverse Primers were tagged at their 5’ end with fluorescent tags (HEX ...
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bioRxiv - Molecular Biology 2021Quote: ... All primers were purchased from Sigma (St. Louis, MO). Controls were same-age same-sex mice of the same background (C57BL/6J ...
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bioRxiv - Physiology 2022Quote: ... The oligonucleotide sequences of the synthesized primers (Sigma-Genosys) and the predicted sizes of the PCR products are given below:
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bioRxiv - Developmental Biology 2021Quote: ... Primers were ordered from Sigma-Aldrich (25nmole scale, desalted) and contained Biotin moiety on the 5’ ends for repair donor synthesis ...
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bioRxiv - Biochemistry 2021Quote: ... Primers were ordered from Genosys (Sigma-Aldirch, Burlington, MA) or Integrated DNA Technologies (Coralville ...
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bioRxiv - Neuroscience 2020Quote: ... Primer sequences (purchased from Sigma or Metabion internacional AG) were checked with BLAST (http://blast.ncbi.nlm.nih.gov/Blast.cgi) ...
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bioRxiv - Microbiology 2020Quote: ... and desalted or PAGE-purified oligonucleotide primers (Sigma Aldrich) by following manufacturers’ instructions ...
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bioRxiv - Microbiology 2020Quote: ... The list of qPCR primers (from Sigma-Aldrich, USA) is shown in Table 2 in Supplementary Material.
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bioRxiv - Neuroscience 2020Quote: Primers were ordered from Sigma-Aldrich (Castle Hill, NSW). Optimal primer dilutions and amplification efficiencies had previously been determined by TYP and AKS ...
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bioRxiv - Neuroscience 2022Quote: ... qPCR was performed using primers specific for actin (Sigma) and SRY (Thermofisher) ...
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bioRxiv - Molecular Biology 2022Quote: Primers used in this study were synthesized by Sigma/Merck ...
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Telomerase deficiency in humans is associated with systemic age-related changes in energy metabolismbioRxiv - Cell Biology 2022Quote: ... These standards primers were HPLC purified (Sigma Aldrich, US) and resuspended to a 100μM stock in molecular grade water ...
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bioRxiv - Cancer Biology 2022Quote: ... with random hexamer or oligo(dT) primers (Sigma-Aldrich). The cDNA samples were subjected to qPCR as described previously (41 ...
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bioRxiv - Pathology 2022Quote: ... and 1 μL of 5 μM primer stock (Sigma, see Supplementary table 1 for sequences ...
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bioRxiv - Biochemistry 2021Quote: ... All primers and oligonucleotides were obtained (Sigma, Gillingham UK) as salt free purified lyophilized DNA ...
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bioRxiv - Molecular Biology 2020Quote: dPCR primers and modified dPCR probes (Sigma Aldrich, Australia) were used to detect mutation g428c on a magnetic induction cycler (MIC ...
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bioRxiv - Neuroscience 2021Quote: ... The reverse primer harbors a 3xFLAG sequence (Sigma-Aldrich) to allow the expression analysis ...
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bioRxiv - Cancer Biology 2020Quote: ... forward and reverse primers (Sigma; 100-500 nM each) and 1× PerfeCTa® SYBR® Green FastMix (Quantabio ...
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bioRxiv - Microbiology 2022Quote: The sequences of all primers (Sigma or Eurofins Genomics) used in this study are shown in Table S1.
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bioRxiv - Molecular Biology 2019Quote: The qPCR primers were synthesized by Sigma-Aldrich (USA) and the list of primers used in this study is provided in Supplementary Tables 1 and 2.
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bioRxiv - Genomics 2020Quote: ... Gene-specific primers were designed and purchased from Sigma. Primer sequences are detailed in S10 Table ...
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bioRxiv - Neuroscience 2020Quote: ... Primers and probes were finally synthesized by Sigma-Aldrich. All primers were tested on 100 ng of genomic DNA in order to validate experimentally their specificity ...
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bioRxiv - Plant Biology 2020Quote: ... lycopersicum oligonucleotide primers (Table S4) purchased from Sigma-Aldrich. Gene primers and probes were designed using Universal Probe Library (UPL ...
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bioRxiv - Molecular Biology 2019Quote: ... forward and reverse primer (Sigma; 100-500 nM each) and 25 ng of template were used ...
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Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery systembioRxiv - Genetics 2019Quote: Primers used in the study (from IDT or Sigma). gRNA target sequence underlined.
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bioRxiv - Biochemistry 2019Quote: ... The Gibson assembly primers were purchased from Sigma-Aldrich. Mutated MvaT derivatives were generated using the same approach ...